################################## # NO CUT ENZYMES ( 84 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BbvCI BciVI BfrI BlnI BlpI Bpu1102I BsiEI BsiWI BsmBI BsmI Bsp1407I BspHI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII CpoI DraI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV EcoT22I FseI HindIII HpaI KpnI MluI NdeI NheI NotI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 832 GTAGAC GT^[AC][GT]AC AccIII 1813 TCCGGA T^CCGGA AcyI 42 GGCGCC G[GA]^CG[CT]C AflIII 755 ACATGT A^C[GA][CT]GT Aor51HI 1157 AGCGCT AGC^GCT AspI 1791 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 628 TGCGCA TGC^GCA BbeI 42 GGCGCC GGCGC^C BbrPI 241 CACGTG CAC^GTG BcgI 1034 CGAGCGTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1085 TGATCA T^GATCA BglI 428 GCCCTGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 1995 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 42 GGCGCC G[GA]^CG[CT]C BsaI 1857 GGTCTC GGTCTC(1/5) BseAI 1813 TCCGGA T^CCGGA BspDI 730 ATCGAT AT^CGAT BspEI 1813 TCCGGA T^CCGGA BspLU11I 755 ACATGT A^CATGT BsrDI 1931 GCAATG GCAATG(2/0) BssSI 1277 CACGAG CACGAG(-5/-1) ClaI 730 ATCGAT AT^CGAT DrdI 301 GACACCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1157 AGCGCT AGC^GCT EcoNI 1440 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1288 GAATTC G^AATTC FbaI 1085 TGATCA T^GATCA FspI 628 TGCGCA TGC^GCA Hin1I 42 GGCGCC G[GA]^CG[CT]C HincII 768 GTTGAC GT[CT]^[GA]AC HindII 768 GTTGAC GT[CT]^[GA]AC KasI 42 GGCGCC G^GCGCC KspI 83 CCGCGG CCGC^GG MamI 1995 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1813 TCCGGA T^CCGGA MspA1I 83 CCGCGG C[AC]G^C[GT]G NarI 42 GGCGCC GG^CGCC NcoI 1550 CCATGG C^CATGG NruI 1698 TCGCGA TCG^CGA PflFI 1791 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 241 CACGTG CAC^GTG PmlI 241 CACGTG CAC^GTG PpuMI 1579 AGGACCC [GA]G^G[AT]CC[CT] SacII 83 CCGCGG CCGC^GG SexAI 410 ACCTGGT A^CC[AT]GGT SfoI 42 GGCGCC GGC^GCC SmaI 1800 CCCGGG CCC^GGG Tth111I 1791 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1940 CCATGCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1800 CCCGGG C^CCGGG XmaI 1800 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AfaI 819 GTAC GT^AC 1230 GTAC AocI 807 CCTGAGG CC^T[ACGT]AGG 1400 CCTCAGG ApaI 1235 GGGCCC GGGCC^C 1621 GGGCCC AspHI 523 GAGCAC G[AT]GC[AT]^C 1096 GTGCTC BalI 263 TGGCCA TGG^CCA 1124 TGGCCA BanI 42 GGCGCC G^G[CT][GA]CC 874 GGTGCC BbsI 586 GAAGAC GAAGAC(2/6) 1651 GAAGAC BfaI 580 CTAG C^TAG 2089 CTAG BpuAI 586 GAAGAC GAAGAC(2/6) 1651 GAAGAC BsaAI 241 CACGTG [CT]AC^GT[GA] 1708 CACGTA BseRI 475 GAGGAG GAGGAG(10/8) 1897 GAGGAG BsiHKAI 523 GAGCAC G[AT]GC[AT]^C 1096 GTGCTC BsrBI 123 GAGCGG CCGCTC(-3/-3) 139 GAGCGG BstXI 1361 CCAAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1529 CCAATTGTGTGG Bsu36I 807 CCTGAGG CC^T[ACGT]AGG 1400 CCTCAGG DraIII 663 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 665 CACAGTGTG DsaI 83 CCGCGG C^C[GA][CT]GG 1550 CCATGG EarI 285 GAAGAG CTCTTC(1/4) 918 GAAGAG Eco81I 807 CCTGAGG CC^T[ACGT]AGG 1400 CCTCAGG Ksp632I 285 GAAGAG CTCTTC(1/4) 918 GAAGAG MaeI 580 CTAG C^TAG 2089 CTAG MfeI 1530 CAATTG C^AATTG 1962 CAATTG MluNI 263 TGGCCA TGG^CCA 1124 TGGCCA MscI 263 TGGCCA TGG^CCA 1124 TGGCCA MslI 744 CATAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1560 CATCCCCGTG MunI 1530 CAATTG C^AATTG 1962 CAATTG NaeI 3 GCCGGC GCC^GGC 36 GCCGGC NgoMIV 3 GCCGGC G^CCGGC 36 GCCGGC NspI 755 ACATGT [GA]CATG^[CT] 1820 GCATGT PflMI 822 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1116 CCACATCCTGG RsaI 819 GTAC GT^AC 1230 GTAC SspI 928 AATATT AAT^ATT 1504 AATATT StuI 1256 AGGCCT AGG^CCT 1457 AGGCCT Tsp45I 310 GTCAC ^GT[GC]AC 994 GTCAC Van91I 822 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1116 CCACATCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcsI AluI AlwI AlwNI ApoI AvaI AvaII BamHI BanII BbvI BcnI BglII BmyI BpmI BsaJI BsaWI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII