Restriction map for
pFN21AE3282
(Circular) (Six-base) MAPSORT of: pFN21AE3282.seq Check: 8680 from: 1 to: 6847
pFN21AE3282 6847 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2541 2541
Size: 6847
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4657 278
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2838 4190 1937
Size: 901 1352 4594
Fragments arranged by size:
4594 1352 901
Acc65I G'GTAC_C
Cuts at: 4168 4168
Size: 6847
AclI AA'CG_TT
Cuts at: 1563 4974 5347 1563
Size: 3411 373 3063
Fragments arranged by size:
3411 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3470 4910 6294 6562 1632
Size: 1838 1440 1384 268 1917
Fragments arranged by size:
1917 1838 1440 1384 268
AfeI AGC'GCT
Cuts at: 3164 3164
Size: 6847
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6650
AflIII A'CryG_T
Cuts at: 1203 2760 5746 6723 1203
Size: 1557 2986 977 1327
Fragments arranged by size:
2986 1557 1327 977
AgeI A'CCGG_T
Cuts at: 5678 5678
Size: 6847
AhdI GACnn_n'nnGTC
Cuts at: 5576 5576
Size: 6847
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6847
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2465 4065 1184
Size: 1281 1600 3966
Fragments arranged by size:
3966 1600 1281
AlwNI CAG_nnn'CTG
Cuts at: 2947 3201 3955 4039 4590 6162 2947
Size: 254 754 84 551 1572 3632
Fragments arranged by size:
3632 1572 754 551 254 84
ApaI G_GGCC'C
Cuts at: 3244 3630 3244
Size: 386 6461
ApaLI G'TGCA_C
Cuts at: 4904 6060 4904
Size: 1156 5691
ApoI r'AATT_y
Cuts at: 1493 2575 3293 3781 4156 4382 4534 6643 1493
Size: 1082 718 488 375 226 152 2109 1697
Fragments arranged by size:
2109 1697 1082 718 488 375 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5401 160
Size: 872 4369 1606
Fragments arranged by size:
4369 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
AvaI C'yCGr_G
Cuts at: 1870 1943 3037 3589 3805 4172 1870
Size: 73 1094 552 216 367 4545
Fragments arranged by size:
4545 1094 552 367 216 73
BamHI G'GATC_C
Cuts at: 2640 2656 2914 4177 4544 2640
Size: 16 258 1263 367 4943
Fragments arranged by size:
4943 1263 367 258 16
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 2879 4168 5623 618
Size: 325 217 627 260 832 1289 1455 1842
Fragments arranged by size:
1842 1455 1289 832 627 325 260 217
BanII G_rGCy'C
Cuts at: 1391 2031 2219 3038 3244 3615 3630 3965 4166
Size: 640 188 819 206 371 15 335 201
Cuts at: 4166 1391
Size: 4072
Fragments arranged by size:
4072 819 640 371 335 206 201 188
15
BbeI G_GCGC'C
Cuts at: 2051 2051
Size: 6847
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2598 3663 928
Size: 971 699 1065 4112
Fragments arranged by size:
4112 1065 971 699
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3028 3062 5038 5072 1903
Size: 34 50 34 1007 34 1976 34 3678
Fragments arranged by size:
3678 1976 1007 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4739 5949 4739
Size: 1210 5637
BclI T'GATC_A
Cuts at: 1548 3090 1548
Size: 1542 5305
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2439 5458
Size: 107 122 71 631 605 337 430 3019
Cuts at: 5458 136
Size: 1525
Fragments arranged by size:
3019 1525 631 605 430 337 122 107
71
BglII A'GATC_T
Cuts at: 2457 3028 3286 6842 2457
Size: 571 258 3556 2462
Fragments arranged by size:
3556 2462 571 258
BlpI GC'TnA_GC
Cuts at: 4264 4264
Size: 6847
Bme1580I G_kGCm'C
Cuts at: 1790 2139 2435 2991 3244 3630 4908 6064 1790
Size: 349 296 556 253 386 1278 1156 2573
Fragments arranged by size:
2573 1278 1156 556 386 349 296 253
BmgBI CAC'GTC
Cuts at: 1318 3088 1318
Size: 1770 5077
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4135 4578 5536
Size: 416 53 83 399 24 2718 443 958
Cuts at: 5536 442
Size: 1753
Fragments arranged by size:
2718 1753 958 443 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2498 3179 4085 5507 1239
Size: 1259 681 906 1422 2579
Fragments arranged by size:
2579 1422 1259 906 681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4972 5837 6135 4972
Size: 865 298 5684
Fragments arranged by size:
5684 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3868 5510 882
Size: 617 2369 1642 2219
Fragments arranged by size:
2369 2219 1642 617
BsaAI yAC'GTr
Cuts at: 493 1204 2248 3715 4638 493
Size: 711 1044 1467 923 2702
Fragments arranged by size:
2702 1467 1044 923 711
BsaBI GATnn'nnATC
Cuts at: 4004 6841 4004
Size: 2837 4010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2048 4654 5036 5671
Size: 53 83 186 936 515 2606 382 635
Cuts at: 5671 275
Size: 1451
Fragments arranged by size:
2606 1451 936 635 515 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2911 3456 3607 3818 3925 5280 5678 5952 6099
Size: 545 151 211 107 1355 398 274 147
Cuts at: 6099 6510 2911
Size: 411 3248
Fragments arranged by size:
3248 1355 545 411 398 274 211 151
147 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3896 3926 1178
Size: 30 165 30 2493 30 4099
Fragments arranged by size:
4099 2493 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2495 3917 1186
Size: 1309 1422 4116
Fragments arranged by size:
4116 1422 1309
BseYI C'CCAG_C
Cuts at: 2206 3067 3932 6050 6546 2206
Size: 861 865 2118 496 2507
Fragments arranged by size:
2507 2118 865 861 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 2519 2679 3035 3326 1163
Size: 573 492 291 160 356 291 4684
Fragments arranged by size:
4684 573 492 356 291 291 160
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4297 5058 6086 1540
Size: 275 186 2296 761 1028 2301
Fragments arranged by size:
2301 2296 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2532 3105 4908 4993 6064 2532
Size: 573 1803 85 1071 3315
Fragments arranged by size:
3315 1803 1071 573 85
BsmI GAATG_Cn'
Cuts at: 4358 4451 4358
Size: 93 6754
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2139 2219 2435 2532 2991 3038
Size: 399 241 108 80 216 97 459 47
Cuts at: 3038 3105 3244 3615 3630 3965 4166 4908 4993
Size: 67 139 371 15 335 201 742 85
Cuts at: 4993 6064 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 459 399 371 335 241
216 201 139 108 97 85 80 67
47 15
BspEI T'CCGG_A
Cuts at: 3818 6510 3818
Size: 2692 4155
BspHI T'CATG_A
Cuts at: 4736 6466 4736
Size: 1730 5117
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2334 2541 3393 4202 844
Size: 1490 207 852 809 3489
Fragments arranged by size:
3489 1490 852 809 207
BsrBI CCG'CTC
Cuts at: 1394 1823 2130 2146 4734 1394
Size: 429 307 16 2588 3507
Fragments arranged by size:
3507 2588 429 307 16
BsrDI GCAATG_nn'
Cuts at: 65 1248 3943 5342 5516 65
Size: 1183 2695 1399 174 1396
Fragments arranged by size:
2695 1399 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2008 2041 3987 5491 5678 1700
Size: 110 198 33 1946 1504 187 2869
Fragments arranged by size:
2869 1946 1504 198 187 110 33
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6847
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6847
BssSI C'ACGA_G
Cuts at: 3282 4907 5919 3282
Size: 1625 1012 4210
Fragments arranged by size:
4210 1625 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6847
BstBI TT'CG_AA
Cuts at: 5651 5651
Size: 6847
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6847
BstXI CCAn_nnnn'nTGG
Cuts at: 3373 3541 3373
Size: 168 6679
BstYI r'GATC_y
Cuts at: 1972 2343 2457 2640 2656 2914 3028 3286 4177
Size: 371 114 183 16 258 114 258 891
Cuts at: 4177 4544 4929 4946 6398 6484 6496 6842 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 891 385 371 367 346 258
258 183 114 114 86 17 16 12
Bsu36I CC'TnA_GG
Cuts at: 2813 3406 2813
Size: 593 6254
BtgI C'CryG_G
Cuts at: 513 1065 2088 3555 513
Size: 552 1023 1467 3805
Fragments arranged by size:
3805 1467 1023 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6108
Fragments arranged by size:
6108 683 56
BtsI GCAGTG_nn'
Cuts at: 4082 4363 5157 5177 4082
Size: 281 794 20 5752
Fragments arranged by size:
5752 794 281 20
ClaI AT'CG_AT
Cuts at: 1554 2736 1554
Size: 1182 5665
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6812
DraI TTT'AAA
Cuts at: 4151 4151
Size: 6847
DraIII CAC_nnn'GTG
Cuts at: 2673 2675 2673
Size: 2 6845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2312 5854 809
Size: 507 996 3542 1802
Fragments arranged by size:
3542 1802 996 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2268 3129
Size: 54 1425 50 275 18 249 189 861
Cuts at: 3129 4122 4294 5183 6812 8
Size: 993 172 889 1629 43
Fragments arranged by size:
1629 1425 993 889 861 275 249 189
172 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4294 1537
Size: 275 2482 4090
Fragments arranged by size:
4090 2482 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2285 2918 4777 1353
Size: 932 633 1859 3423
Fragments arranged by size:
3423 1859 932 633
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2348 5434 5808 5954 387
Size: 1064 138 759 3086 374 146 1280
Fragments arranged by size:
3086 1280 1064 759 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2498 3179 3470 4085 4910 5507 6294
Size: 393 866 681 291 615 825 597 787
Cuts at: 6294 6562 1239
Size: 268 1524
Fragments arranged by size:
1524 866 825 787 681 615 597 393
291 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3449 3449
Size: 6847
EcoO109I rG'GnC_Cy
Cuts at: 3240 3241 3585 3626 3907 4059 3240
Size: 1 344 41 281 152 6028
Fragments arranged by size:
6028 344 281 152 41 1
EcoRI G'AATT_C
Cuts at: 3293 4156 4534 3293
Size: 863 378 5606
Fragments arranged by size:
5606 863 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3493 3493
Size: 6847
FspI TGC'GCA
Cuts at: 2635 5353 2635
Size: 2718 4129
HaeII r_GCGC'y
Cuts at: 1678 2051 3166 4030 5994 1678
Size: 373 1115 864 1964 2531
Fragments arranged by size:
2531 1964 1115 864 373
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2499 2531 2729 2761 3271
Size: 32 135 32 927 32 198 32 510
Cuts at: 3271 3303 5488 5520 5562 5594 1373
Size: 32 2185 32 42 32 2626
Fragments arranged by size:
2626 2185 927 510 198 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2775 4191 4437 669
Size: 988 281 837 1416 246 3079
Fragments arranged by size:
3079 1416 988 837 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6847
HpaI GTT'AAC
Cuts at: 4437 4437
Size: 6847
KasI G'GCGC_C
Cuts at: 2047 2047
Size: 6847
KpnI G_GTAC'C
Cuts at: 4172 4172
Size: 6847
MfeI C'AATT_G
Cuts at: 3535 3967 4446 3535
Size: 432 479 5936
Fragments arranged by size:
5936 479 432
MluI A'CGCG_T
Cuts at: 6723 6723
Size: 6847
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2530 3132 3354 3878 5961 6145 1305
Size: 1225 602 222 524 2083 184 2007
Fragments arranged by size:
2083 2007 1225 602 524 222 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2270 3131 10
Size: 54 1425 343 438 861 3726
Fragments arranged by size:
3726 1425 861 438 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2753 3569 4805 5164 5323 518
Size: 648 1587 816 1236 359 159 2042
Fragments arranged by size:
2042 1587 1236 816 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2090 4263 4940 6088 6333 1606
Size: 484 2173 677 1148 245 2120
Fragments arranged by size:
2173 2120 1148 677 484 245
NaeI GCC'GGC
Cuts at: 2010 2043 2010
Size: 33 6814
NarI GG'CG_CC
Cuts at: 2048 2048
Size: 6847
NcoI C'CATG_G
Cuts at: 513 1065 3555 513
Size: 552 2490 3805
Fragments arranged by size:
3805 2490 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6847
NgoMIV G'CCGG_C
Cuts at: 2008 2041 2008
Size: 33 6814
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6847
NotI GC'GGCC_GC
Cuts at: 4294 4294
Size: 6847
NruI TCG'CGA
Cuts at: 3705 3705
Size: 6847
NspI r_CATG'y
Cuts at: 1132 1229 2764 3829 4205 5750 1132
Size: 97 1535 1065 376 1545 2229
Fragments arranged by size:
2229 1545 1535 1065 376 97
PasI CC'CwG_GG
Cuts at: 1348 2435 1348
Size: 1087 5760
PciI A'CATG_T
Cuts at: 2760 5746 2760
Size: 2986 3861
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2833 3127 1108
Size: 1725 294 4828
Fragments arranged by size:
4828 1725 294
PfoI T'CCnGG_A
Cuts at: 2904 3189 2904
Size: 285 6562
PmeI GTTT'AAAC
Cuts at: 4151 4151
Size: 6847
PmlI CAC'GTG
Cuts at: 2248 2248
Size: 6847
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3374 3406 4916 4948 6429 6461 3374
Size: 32 1510 32 1481 32 3760
Fragments arranged by size:
3760 1510 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3585 3585
Size: 6847
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6847
PsiI TTA'TAA
Cuts at: 4417 4417
Size: 6847
PspOMI G'GGCC_C
Cuts at: 3240 3626 3240
Size: 386 6461
PstI C_TGCA'G
Cuts at: 830 4044 4054 4089 4199 830
Size: 3214 10 35 110 3478
Fragments arranged by size:
3478 3214 110 35 10
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6847
SacII CC_GC'GG
Cuts at: 2091 2091
Size: 6847
SalI G'TCGA_C
Cuts at: 1936 4189 1936
Size: 2253 4594
SbfI CC_TGCA'GG
Cuts at: 4199 4199
Size: 6847
ScaI AGT'ACT
Cuts at: 5095 6807 5095
Size: 1712 5135
SexAI A'CCwGG_T
Cuts at: 2415 2415
Size: 6847
SfcI C'TryA_G
Cuts at: 826 1045 3742 4040 4050 4085 4195 5330 6011
Size: 219 2697 298 10 35 110 1135 681
Cuts at: 6011 6202 826
Size: 191 1471
Fragments arranged by size:
2697 1471 1135 681 298 219 191 110
35 10
SfoI GGC'GCC
Cuts at: 2049 2049
Size: 6847
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6847
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6847
SmaI CCC'GGG
Cuts at: 1872 3807 4174 1872
Size: 1935 367 4545
Fragments arranged by size:
4545 1935 367
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4951 5852 6114 820
Size: 197 926 3008 901 262 1553
Fragments arranged by size:
3008 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4638 493
Size: 4145 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6847
SphI G_CATG'C
Cuts at: 1132 4205 1132
Size: 3073 3774
SspI AAT'ATT
Cuts at: 5 52 2935 3511 4771 5
Size: 47 2883 576 1260 2081
Fragments arranged by size:
2883 2081 1260 576 47
StuI AGG'CCT
Cuts at: 1517 3263 3464 1517
Size: 1746 201 4900
Fragments arranged by size:
4900 1746 201
StyI C'CwwG_G
Cuts at: 513 1065 2226 3246 3555 4286 513
Size: 552 1161 1020 309 731 3074
Fragments arranged by size:
3074 1161 1020 731 552 309
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2113 3074 4874 4891 5044 5229 5674
Size: 1255 533 961 1800 17 153 185 445
Cuts at: 5674 325
Size: 1498
Fragments arranged by size:
1800 1498 1255 961 533 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3234 5093 6805
Size: 274 80 33 51 1443 1257 1859 1712
Cuts at: 6805 96
Size: 138
Fragments arranged by size:
1859 1712 1443 1257 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3896 3928 6807 6839 3896
Size: 32 2879 32 3904
Fragments arranged by size:
3904 2879 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3799 1316
Size: 2483 4364
XbaI T'CTAG_A
Cuts at: 4183 4183
Size: 6847
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3952 3952
Size: 6847
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6847
XmaI C'CCGG_G
Cuts at: 1870 3805 4172 1870
Size: 1935 367 4545
Fragments arranged by size:
4545 1935 367
XmnI GAAnn'nnTTC
Cuts at: 4976 4976
Size: 6847
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4655 276
Size: 53 83 186 936 3121 2468
Fragments arranged by size:
3121 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI NsiI
PacI PspXI PsrI PvuII RsrII SacI SanDI SapI
SfiI SrfI SwaI