##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbsI        BciVI       BclI        BfaI        BfrI        
BglII       BlnI        BpuAI       BsaBI       BsaI        
BsaWI       BseAI       BsiWI       BsmAI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EarI        Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     EcoT22I     
FbaI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     MaeI        MaeIII      
MamI        MfeI        MflI        MluI        MroI        
MseI        MslI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tsp45I      Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

Aor51HI       647  AGCGCT               AGC^GCT
Asp700I       267  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         340  GTGCTC               G[AT]GC[AT]^C
BalI         1046  TGGCCA               TGG^CCA
BbrPI        1036  CACGTG               CAC^GTG
BbvCI         204  CCTCAGC              CCTCAGC(-5/-2)
BcgI          238  GCAGACGAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI        1036  CACGTG               [CT]AC^GT[GA]
BseRI        1081  GAGGAG               GAGGAG(10/8)
BsiHKAI       340  GTGCTC               G[AT]GC[AT]^C
BspMI         326  ACCTGC               ACCTGC(4/8)
BsrBI         832  CCGCTC               CCGCTC(-3/-3)
BstAPI        429  GCAGCACTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        252  CATCC                GGATG(2/0)
Eco47III      647  AGCGCT               AGC^GCT
EcoNI         795  CCTGGCGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          252  CATCC                GGATG(9/13)
FseI          213  GGCCGGCC             GGCCGG^CC
HphI          971  TCACC                GGTGA(8/7)
MboII         689  TCTTC                GAAGA(8/7)
MluNI        1046  TGGCCA               TGG^CCA
MscI         1046  TGGCCA               TGG^CCA
PmaCI        1036  CACGTG               CAC^GTG
PmlI         1036  CACGTG               CAC^GTG
PpuMI         767  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          996  CTGCAG               CTGCA^G
SfcI          996  CTGCAG               C^T[GA][CT]AG
TspRI        1043  CACTG                CA[GC]TG[ACGT][ACGT]^
XmnI          267  GAACGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AluI          374  AGCT                 AG^CT
              596  AGCT                
BglI          209  GCCCGGCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              930  GCCGCTGGGGC         
BlpI          310  GCTCAGC              GC^T[ACGT]AGC
              342  GCTCAGC             
Bpu1102I      310  GCTCAGC              GC^T[ACGT]AGC
              342  GCTCAGC             
BsgI          328  CTGCAC               GTGCAG(16/14)
              427  GTGCAG              
CelII         310  GCTCAGC              GC^T[ACGT]AGC
              342  GCTCAGC             
HinfI         244  GAGTC                G^A[ACGT]TC
             1005  GACTC               
MaeII         962  ACGT                 A^CGT
             1037  ACGT                
NaeI          109  GCCGGC               GCC^GGC
              214  GCCGGC              
NgoMIV        109  GCCGGC               G^CCGGC
              214  GCCGGC              
PleI         1005  GACTC                GAGTC(4/5)
              244  GAGTC               
TaqI          620  TCGA                 T^CGA
              980  TCGA                
TthHB8I       620  TCGA                 T^CGA
              980  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        ApaI        
AvaI        AvaII       BanI        BanII       BbeI        
BbvI        BcnI        BmyI        BpmI        BsaHI       
BsaJI       BsiEI       BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrFI       BsrI        BssHII      
BssKI       BstNI       BstUI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EagI        EclXI       Eco52I      
EcoO109I    EcoRII      FauI        Fnu4HI      HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HpaII       ItaI        KasI        KspI        
MboI        MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NarI        NciI        NdeII       
NlaIII      NlaIV       RsaI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfoI        SmaI        
TseI        XmaCI       XmaI