(Circular) (Six-base) MAPSORT of: pFN21AB8983.seq Check: 2160 from: 1 to: 5857 pFN21AB8983 5857 bp With 182 enzymes: SgfI * December 8, 2014 13:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3667 278 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3200 1937 Size: 1263 4594 Acc65I G'GTAC_C Cuts at: 3178 3178 Size: 5857 AclI AA'CG_TT Cuts at: 1563 3984 4357 1563 Size: 2421 373 3063 Fragments arranged by size: 3063 2421 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3920 5304 5572 1632 Size: 2288 1384 268 1917 Fragments arranged by size: 2288 1917 1384 268 AfeI AGC'GCT Cuts at: 2654 2654 Size: 5857 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5660 AflIII A'CryG_T Cuts at: 1203 4756 5733 1203 Size: 3553 977 1327 Fragments arranged by size: 3553 1327 977 AgeI A'CCGG_T Cuts at: 4688 4688 Size: 5857 AhdI GACnn_n'nnGTC Cuts at: 4586 4586 Size: 5857 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5857 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5857 AlwNI CAG_nnn'CTG Cuts at: 3600 5172 3600 Size: 1572 4285 ApaI G_GGCC'C Cuts at: 2074 2946 3065 2074 Size: 872 119 4866 Fragments arranged by size: 4866 872 119 ApaLI G'TGCA_C Cuts at: 3914 5070 3914 Size: 1156 4701 ApoI r'AATT_y Cuts at: 1493 3166 3392 3544 5653 1493 Size: 1673 226 152 2109 1697 Fragments arranged by size: 2109 1697 1673 226 152 AseI AT'TA_AT Cuts at: 160 1032 4411 160 Size: 872 3379 1606 Fragments arranged by size: 3379 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 AvaI C'yCGr_G Cuts at: 1870 1943 2074 2189 2527 2555 2648 2714 2906 Size: 73 131 115 338 28 93 66 192 Cuts at: 2906 3026 3182 1870 Size: 120 156 4545 Fragments arranged by size: 4545 338 192 156 131 120 115 93 73 66 28 BamHI G'GATC_C Cuts at: 3187 3554 3187 Size: 367 5490 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2615 2710 2833 2858 3178 Size: 325 217 627 828 95 123 25 320 Cuts at: 3178 4633 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 828 627 325 320 217 123 95 25 BanII G_rGCy'C Cuts at: 1391 2074 2514 2679 2797 2946 3033 3065 3176 Size: 683 440 165 118 149 87 32 111 Cuts at: 3176 1391 Size: 4072 Fragments arranged by size: 4072 683 440 165 149 118 111 87 32 BbeI G_GCGC'C Cuts at: 2619 2714 2837 2619 Size: 95 123 5639 Fragments arranged by size: 5639 123 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4886 BbvCI CC'TCA_GC Cuts at: 2210 2210 Size: 5857 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2232 2266 4048 4082 1903 Size: 34 50 34 211 34 1782 34 3678 Fragments arranged by size: 3678 1782 211 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3749 4959 3749 Size: 1210 4647 BclI T'GATC_A Cuts at: 1548 1548 Size: 5857 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2220 2941 4468 Size: 107 122 71 631 605 548 721 1527 Cuts at: 4468 136 Size: 1525 Fragments arranged by size: 1527 1525 721 631 605 548 122 107 71 BglII A'GATC_T Cuts at: 5852 5852 Size: 5857 BlpI GC'TnA_GC Cuts at: 2316 2348 3274 2316 Size: 32 926 4899 Fragments arranged by size: 4899 926 32 Bme1580I G_kGCm'C Cuts at: 1790 2074 2870 2946 3065 3918 5074 1790 Size: 284 796 76 119 853 1156 2573 Fragments arranged by size: 2573 1156 853 796 284 119 76 BmgBI CAC'GTC Cuts at: 1318 2968 1318 Size: 1650 4207 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3068 3588 4546 Size: 416 53 83 399 24 1651 520 958 Cuts at: 4546 442 Size: 1753 Fragments arranged by size: 1753 1651 958 520 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5857 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2528 2693 2942 4517 1239 Size: 1289 165 249 1575 2579 Fragments arranged by size: 2579 1575 1289 249 165 Bpu10I CC'TnA_GC Cuts at: 2210 2813 2210 Size: 603 5254 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3982 4847 5145 3982 Size: 865 298 4694 Fragments arranged by size: 4694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4520 882 Size: 617 3021 2219 Fragments arranged by size: 3021 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3043 3648 493 Size: 711 1839 605 2702 Fragments arranged by size: 2702 1839 711 605 BsaBI GATnn'nnATC Cuts at: 5851 5851 Size: 5857 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2573 2616 2711 2834 Size: 53 83 186 936 1040 43 95 123 Cuts at: 2834 3021 3117 3664 4046 4681 275 Size: 187 96 547 382 635 1451 Fragments arranged by size: 1451 1040 936 635 547 382 187 186 123 96 95 83 53 43 BsaWI w'CCGG_w Cuts at: 4290 4688 4962 5109 5520 4290 Size: 398 274 147 411 4627 Fragments arranged by size: 4627 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5632 Fragments arranged by size: 5632 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3101 1186 Size: 1915 3942 BseYI C'CCAG_C Cuts at: 2159 2359 2611 2938 5060 5556 2159 Size: 200 252 327 2122 496 2460 Fragments arranged by size: 2460 2122 496 327 252 200 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2318 2453 1163 Size: 573 582 135 4567 Fragments arranged by size: 4567 582 573 135 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2220 2224 2471 3307 4068 5096 Size: 275 186 219 4 247 836 761 1028 Cuts at: 5096 1540 Size: 2301 Fragments arranged by size: 2301 1028 836 761 275 247 219 186 4 BsiHKAI G_wGCw'C Cuts at: 2349 3918 4003 5074 2349 Size: 1569 85 1071 3132 Fragments arranged by size: 3132 1569 1071 85 BsmI GAATG_Cn' Cuts at: 3368 3461 3368 Size: 93 5764 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2349 2514 2679 2797 2870 2946 Size: 399 284 275 165 165 118 73 76 Cuts at: 2946 3033 3065 3176 3918 4003 5074 1391 Size: 87 32 111 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 284 275 165 165 118 111 87 85 76 73 32 BspEI T'CCGG_A Cuts at: 5520 5520 Size: 5857 BspHI T'CATG_A Cuts at: 3746 5476 3746 Size: 1730 4127 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2340 3212 844 Size: 1496 872 3489 Fragments arranged by size: 3489 1496 872 BsrBI CCG'CTC Cuts at: 1394 1823 2839 3744 1394 Size: 429 1016 905 3507 Fragments arranged by size: 3507 1016 905 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4352 4526 65 Size: 1183 3104 174 1396 Fragments arranged by size: 3104 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2114 2219 2466 4501 4688 1700 Size: 110 304 105 247 2035 187 2869 Fragments arranged by size: 2869 2035 304 247 187 110 105 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5857 BssHII G'CGCG_C Cuts at: 1925 2489 2724 2742 3067 1925 Size: 564 235 18 325 4715 Fragments arranged by size: 4715 564 325 235 18 BssSI C'ACGA_G Cuts at: 3917 4929 3917 Size: 1012 4845 BstAPI GCAn_nnn'nTGC Cuts at: 794 2440 794 Size: 1646 4211 BstBI TT'CG_AA Cuts at: 4661 4661 Size: 5857 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5857 BstYI r'GATC_y Cuts at: 1972 3187 3554 3939 3956 5408 5494 5506 5852 Size: 1215 367 385 17 1452 86 12 346 Cuts at: 5852 1972 Size: 1977 Fragments arranged by size: 1977 1452 1215 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2224 2568 2706 2829 513 Size: 552 1159 344 138 123 3541 Fragments arranged by size: 3541 1159 552 344 138 123 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5118 Fragments arranged by size: 5118 683 56 BtsI GCAGTG_nn' Cuts at: 3373 4167 4187 3373 Size: 794 20 5043 Fragments arranged by size: 5043 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5857 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5822 DraI TTT'AAA Cuts at: 3161 3161 Size: 5857 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4864 809 Size: 507 3548 1802 Fragments arranged by size: 3548 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2217 2221 2468 Size: 54 1425 50 275 18 387 4 247 Cuts at: 2468 3051 3304 4193 5822 8 Size: 583 253 889 1629 43 Fragments arranged by size: 1629 1425 889 583 387 275 253 247 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 2217 2221 2468 3304 1537 Size: 275 405 4 247 836 4090 Fragments arranged by size: 4090 836 405 275 247 4 EarI CTCTTCn'nnn_ Cuts at: 1353 3787 1353 Size: 2434 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2405 2819 4444 4818 4964 387 Size: 1064 138 816 414 1625 374 146 1280 Fragments arranged by size: 1625 1280 1064 816 414 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2528 2693 2942 3920 4517 5304 5572 Size: 393 896 165 249 978 597 787 268 Cuts at: 5572 1239 Size: 1524 Fragments arranged by size: 1524 978 896 787 597 393 268 249 165 EcoNI CCTnn'n_nnAGG Cuts at: 2804 2804 Size: 5857 EcoO109I rG'GnC_Cy Cuts at: 2071 2773 2911 2942 2071 Size: 702 138 31 4986 Fragments arranged by size: 4986 702 138 31 EcoRI G'AATT_C Cuts at: 3166 3544 3166 Size: 378 5479 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2419 2419 Size: 5857 FseI GG_CCGG'CC Cuts at: 2223 2223 Size: 5857 FspI TGC'GCA Cuts at: 4363 4363 Size: 5857 HaeII r_GCGC'y Cuts at: 1678 2203 2540 2619 2656 2714 2813 2837 2931 Size: 525 337 79 37 58 99 24 94 Cuts at: 2931 5004 1678 Size: 2073 2531 Fragments arranged by size: 2531 2073 525 337 99 94 79 58 37 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4498 4530 4572 4604 1373 Size: 32 135 32 2926 32 42 32 2626 Fragments arranged by size: 2926 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3201 3447 669 Size: 988 281 1263 246 3079 Fragments arranged by size: 3079 1263 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5857 HpaI GTT'AAC Cuts at: 3447 3447 Size: 5857 KasI G'GCGC_C Cuts at: 2615 2710 2833 2615 Size: 95 123 5639 Fragments arranged by size: 5639 123 95 KpnI G_GTAC'C Cuts at: 3182 3182 Size: 5857 MfeI C'AATT_G Cuts at: 3456 3456 Size: 5857 MluI A'CGCG_T Cuts at: 5733 5733 Size: 5857 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3093 4971 5155 1305 Size: 1788 1878 184 2007 Fragments arranged by size: 2007 1878 1788 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3053 10 Size: 54 1425 343 1221 2814 Fragments arranged by size: 2814 1425 1221 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3815 4174 4333 518 Size: 648 2649 359 159 2042 Fragments arranged by size: 2649 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2093 2226 2570 2611 2708 2831 2893 2920 Size: 487 133 344 41 97 123 62 27 Cuts at: 2920 2938 3273 3950 5098 5343 1606 Size: 18 335 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 487 344 335 245 133 123 97 62 41 27 18 NaeI GCC'GGC Cuts at: 2116 2221 2116 Size: 105 5752 NarI GG'CG_CC Cuts at: 2616 2711 2834 2616 Size: 95 123 5639 Fragments arranged by size: 5639 123 95 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5305 NdeI CA'TA_TG Cuts at: 387 387 Size: 5857 NgoMIV G'CCGG_C Cuts at: 2114 2219 2114 Size: 105 5752 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5857 NotI GC'GGCC_GC Cuts at: 3304 3304 Size: 5857 NspI r_CATG'y Cuts at: 1132 1229 3215 4760 1132 Size: 97 1986 1545 2229 Fragments arranged by size: 2229 1986 1545 97 PasI CC'CwG_GG Cuts at: 1348 2863 1348 Size: 1515 4342 PciI A'CATG_T Cuts at: 4756 4756 Size: 5857 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5857 PmeI GTTT'AAAC Cuts at: 3161 3161 Size: 5857 PmlI CAC'GTG Cuts at: 3043 3043 Size: 5857 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3926 3958 5439 5471 3926 Size: 32 1481 32 4312 Fragments arranged by size: 4312 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2773 2773 Size: 5857 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5857 PsiI TTA'TAA Cuts at: 3427 3427 Size: 5857 PspOMI G'GGCC_C Cuts at: 2070 2942 3061 2070 Size: 872 119 4866 Fragments arranged by size: 4866 872 119 PstI C_TGCA'G Cuts at: 830 3005 3209 830 Size: 2175 204 3478 Fragments arranged by size: 3478 2175 204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5857 SacII CC_GC'GG Cuts at: 2227 2571 2709 2832 2227 Size: 344 138 123 5252 Fragments arranged by size: 5252 344 138 123 SalI G'TCGA_C Cuts at: 1936 3199 1936 Size: 1263 4594 SanDI GG'GwC_CC Cuts at: 2773 2773 Size: 5857 SbfI CC_TGCA'GG Cuts at: 3209 3209 Size: 5857 ScaI AGT'ACT Cuts at: 4105 5817 4105 Size: 1712 4145 SfcI C'TryA_G Cuts at: 826 1045 3001 3205 4340 5021 5212 826 Size: 219 1956 204 1135 681 191 1471 Fragments arranged by size: 1956 1471 1135 681 219 204 191 SfoI GGC'GCC Cuts at: 2617 2712 2835 2617 Size: 95 123 5639 Fragments arranged by size: 5639 123 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5857 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5857 SmaI CCC'GGG Cuts at: 1872 2076 2529 3028 3184 1872 Size: 204 453 499 156 4545 Fragments arranged by size: 4545 499 453 204 156 SmlI C'TyrA_G Cuts at: 820 1017 1943 3961 4862 5124 820 Size: 197 926 2018 901 262 1553 Fragments arranged by size: 2018 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3648 493 Size: 3155 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5857 SphI G_CATG'C Cuts at: 1132 3215 1132 Size: 2083 3774 SspI AAT'ATT Cuts at: 5 52 3781 5 Size: 47 3729 2081 Fragments arranged by size: 3729 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5857 StyI C'CwwG_G Cuts at: 513 1065 3296 513 Size: 552 2231 3074 Fragments arranged by size: 3074 2231 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3884 3901 4054 4239 4684 325 Size: 1255 2304 17 153 185 445 1498 Fragments arranged by size: 2304 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2850 4103 5815 Size: 274 80 33 51 1443 873 1253 1712 Cuts at: 5815 96 Size: 138 Fragments arranged by size: 1712 1443 1253 873 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5817 5849 5817 Size: 32 5825 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5857 XbaI T'CTAG_A Cuts at: 3193 3193 Size: 5857 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5857 XmaI C'CCGG_G Cuts at: 1870 2074 2527 3026 3182 1870 Size: 204 453 499 156 4545 Fragments arranged by size: 4545 499 453 204 156 XmnI GAAnn'nnTTC Cuts at: 2276 3986 2276 Size: 1710 4147 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3665 276 Size: 53 83 186 936 2131 2468 Fragments arranged by size: 2468 2131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FspAI NruI NsiI PacI PfoI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SrfI SwaI XcmI