Restriction map for
pFN21AE3235
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AhdI
AlwNI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BamHI BbeI BbvCI BcgI
BciVI BfaI BfrI BlnI BsaBI
BsaHI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
Eam1105I Eco47III EcoNI EcoO65I EcoRI
EcoRV FseI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MroI
MseI MunI NarI NdeI NheI
NspV PacI PaeR7I PmeI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SmaI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tru9I Tsp509I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AgeI 1183 ACCGGT A^CCGGT
AviII 1250 TGCGCA TGC^GCA
BbsI 330 GAAGAC GAAGAC(2/6)
BclI 350 TGATCA T^GATCA
BglI 1384 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 969 GCTGAGC GC^T[ACGT]AGC
BpmI 898 CTGGAG CTGGAG(16/14)
Bpu1102I 969 GCTGAGC GC^T[ACGT]AGC
BpuAI 330 GAAGAC GAAGAC(2/6)
BsaWI 1183 ACCGGT [AT]^CCGG[AT]
BspMI 993 GCAGGT ACCTGC(4/8)
BsrBI 846 GAGCGG CCGCTC(-3/-3)
BsrDI 116 GCAATG GCAATG(2/0)
BstAPI 879 GCAGCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 969 GCTGAGC GC^T[ACGT]AGC
DraII 359 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 359 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1487 ATGCAT ATGCA^T
FbaI 350 TGATCA T^GATCA
FspI 1250 TGCGCA TGC^GCA
HaeII 161 GGCGCT [GA]GCGC^[CT]
MaeIII 1616 GTGAC ^GT[ACGT]AC
NaeI 74 GCCGGC GCC^GGC
NgoMIV 74 GCCGGC G^CCGGC
NotI 249 GCGGCCGC GC^GGCCGC
NruI 1038 TCGCGA TCG^CGA
NsiI 1487 ATGCAT ATGCA^T
NspI 1004 GCATGC [GA]CATG^[CT]
PflMI 1558 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1183 ACCGGT A^CCGGT
PvuII 895 CAGCTG CAG^CTG
SapI 981 GAAGAGC GCTCTTC(1/4)
SmlI 505 CTCAAG C^T[CT][GA]AG
SphI 1004 GCATGC GCATG^C
StuI 290 AGGCCT AGG^CCT
TfiI 1505 GAATC G^A[AT]TC
Tsp45I 1616 GTGAC ^GT[GC]AC
Van91I 1558 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 809 CCACCATGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
Alw44I 886 GTGCAC G^TGCAC
1471 GTGCAC
AlwI 31 GATCC GGATC(4/5)
1347 GATCC
AocI 1311 CCTGAGG CC^T[ACGT]AGG
1423 CCTGAGG
ApaLI 886 GTGCAC G^TGCAC
1471 GTGCAC
AspI 1117 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1597 GACGCGGTC
AvaI 59 CCCGAG C^[CT]CG[GA]G
1297 CCCGAG
BalI 341 TGGCCA TGG^CCA
860 TGGCCA
BanI 537 GGTGCC G^G[CT][GA]CC
1258 GGCACC
BbrPI 883 CACGTG CAC^GTG
1342 CACGTG
BglII 668 AGATCT A^GATCT
1433 AGATCT
BsaAI 883 CACGTG [CT]AC^GT[GA]
1342 CACGTG
BsaI 94 GAGACC GGTCTC(1/5)
1030 GAGACC
BseRI 901 GAGGAG GAGGAG(10/8)
1621 GAGGAG
BsgI 1359 CTGCAC GTGCAG(16/14)
877 GTGCAG
BsoBI 59 CCCGAG C^[CT]CG[GA]G
1297 CCCGAG
BssHII 1200 GCGCGC G^CGCGC
1230 GCGCGC
BstYI 668 AGATCT [GA]^GATC[CT]
1433 AGATCT
Bsu36I 1311 CCTGAGG CC^T[ACGT]AGG
1423 CCTGAGG
DraIII 1414 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
1474 CACTCAGTG
DrdI 826 GACATCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1525 GACAAGCGGGTC
EagI 250 CGGCCG C^GGCCG
735 CGGCCG
EarI 525 GAAGAG CTCTTC(1/4)
981 GAAGAG
EclXI 250 CGGCCG C^GGCCG
735 CGGCCG
Eco52I 250 CGGCCG C^GGCCG
735 CGGCCG
Eco81I 1311 CCTGAGG CC^T[ACGT]AGG
1423 CCTGAGG
Ksp632I 525 GAAGAG CTCTTC(1/4)
981 GAAGAG
MflI 668 AGATCT [GA]^GATC[CT]
1433 AGATCT
MluNI 341 TGGCCA TGG^CCA
860 TGGCCA
MscI 341 TGGCCA TGG^CCA
860 TGGCCA
MspA1I 895 CAGCTG C[AC]G^C[GT]G
955 CCGCTG
NcoI 392 CCATGG C^CATGG
1214 CCATGG
PflFI 1117 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1597 GACGCGGTC
PmaCI 883 CACGTG CAC^GTG
1342 CACGTG
PmlI 883 CACGTG CAC^GTG
1342 CACGTG
SacI 652 GAGCTC GAGCT^C
1300 GAGCTC
SgrAI 1182 CACCGGTG C[GA]^CCGG[CT]G
1203 CGCCGGTG
Tth111I 1117 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1597 GACGCGGTC
XhoII 668 AGATCT [GA]^GATC[CT]
1433 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MboI MboII
MnlI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI TspRI
TthHB8I