(Circular) (Six-base) MAPSORT of: pFN21AE3235.seq Check: 3302 from: 1 to: 6349 pFN21AE3235 6349 bp With 182 enzymes: SgfI * March 28, 2012 12:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4159 278 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3692 1937 Size: 1755 4594 Acc65I G'GTAC_C Cuts at: 3670 3670 Size: 6349 AclI AA'CG_TT Cuts at: 1563 4476 4849 1563 Size: 2913 373 3063 Fragments arranged by size: 3063 2913 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2540 3005 4412 5796 6064 1632 Size: 908 465 1407 1384 268 1917 Fragments arranged by size: 1917 1407 1384 908 465 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6152 AflIII A'CryG_T Cuts at: 1203 5248 6225 1203 Size: 4045 977 1327 Fragments arranged by size: 4045 1327 977 AgeI A'CCGG_T Cuts at: 3188 5180 3188 Size: 1992 4357 AhdI GACnn_n'nnGTC Cuts at: 5078 5078 Size: 6349 AleI CACnn'nnGTG Cuts at: 1166 2950 3191 3524 1166 Size: 1784 241 333 3991 Fragments arranged by size: 3991 1784 333 241 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2968 3171 3269 1184 Size: 1784 203 98 4264 Fragments arranged by size: 4264 1784 203 98 AlwNI CAG_nnn'CTG Cuts at: 4092 5664 4092 Size: 1572 4777 ApaLI G'TGCA_C Cuts at: 2891 3476 4406 5562 2891 Size: 585 930 1156 3678 Fragments arranged by size: 3678 1156 930 585 ApoI r'AATT_y Cuts at: 1493 3658 3884 4036 6145 1493 Size: 2165 226 152 2109 1697 Fragments arranged by size: 2165 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4903 160 Size: 872 3871 1606 Fragments arranged by size: 3871 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 AvaI C'yCGr_G Cuts at: 1870 1943 2064 3302 3674 1870 Size: 73 121 1238 372 4545 Fragments arranged by size: 4545 1238 372 121 73 BamHI G'GATC_C Cuts at: 3679 4046 3679 Size: 367 5982 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2542 3263 3670 5125 618 Size: 325 217 627 755 721 407 1455 1842 Fragments arranged by size: 1842 1455 755 721 627 407 325 217 BanII G_rGCy'C Cuts at: 1391 2661 3228 3303 3309 3668 1391 Size: 1270 567 75 6 359 4072 Fragments arranged by size: 4072 1270 567 359 75 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2342 928 Size: 971 443 4935 Fragments arranged by size: 4935 971 443 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4540 4574 1903 Size: 34 2603 34 3678 Fragments arranged by size: 3678 2603 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4241 5451 4241 Size: 1210 5139 BclI T'GATC_A Cuts at: 1548 2355 1548 Size: 807 5542 BfrBI ATG'CAT Cuts at: 3494 3494 Size: 6349 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3395 4960 136 Size: 107 122 71 631 605 1723 1565 1525 Fragments arranged by size: 1723 1565 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2673 3438 6344 2673 Size: 765 2906 2678 Fragments arranged by size: 2906 2678 765 BlpI GC'TnA_GC Cuts at: 2975 3766 2975 Size: 791 5558 Bme1580I G_kGCm'C Cuts at: 1790 2162 2379 2547 2895 3480 4410 5566 1790 Size: 372 217 168 348 585 930 1156 2573 Fragments arranged by size: 2573 1156 930 585 372 348 217 168 BmgBI CAC'GTC Cuts at: 1318 2049 3253 3424 1318 Size: 731 1204 171 4243 Fragments arranged by size: 4243 1204 731 171 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2172 4080 5038 Size: 416 53 83 399 24 755 1908 958 Cuts at: 5038 442 Size: 1753 Fragments arranged by size: 1908 1753 958 755 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6349 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2924 5009 1239 Size: 1685 2085 2579 Fragments arranged by size: 2579 2085 1685 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2495 4474 5339 5637 2495 Size: 1979 865 298 3207 Fragments arranged by size: 3207 1979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2093 3029 5012 882 Size: 617 594 936 1983 2219 Fragments arranged by size: 2219 1983 936 617 594 BsaAI yAC'GTr Cuts at: 493 1204 2890 3349 4140 493 Size: 711 1686 459 791 2702 Fragments arranged by size: 2702 1686 791 711 459 BsaBI GATnn'nnATC Cuts at: 6343 6343 Size: 6349 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4156 4538 5173 275 Size: 53 83 186 936 2623 382 635 1451 Fragments arranged by size: 2623 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3188 4782 5180 5454 5601 6012 3188 Size: 1594 398 274 147 411 3525 Fragments arranged by size: 3525 1594 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2675 2705 3086 3116 1178 Size: 30 165 30 1272 30 381 30 4411 Fragments arranged by size: 4411 1272 381 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2921 3641 1186 Size: 1735 720 3894 Fragments arranged by size: 3894 1735 720 BseYI C'CCAG_C Cuts at: 5552 6048 5552 Size: 496 5853 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2903 3349 1163 Size: 573 1167 446 4163 Fragments arranged by size: 4163 1167 573 446 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2258 2743 3586 3799 4560 5588 Size: 275 186 257 485 843 213 761 1028 Cuts at: 5588 1540 Size: 2301 Fragments arranged by size: 2301 1028 843 761 485 275 257 213 186 BsiHKAI G_wGCw'C Cuts at: 2661 2895 2993 3096 3309 3411 3480 4410 4495 Size: 234 98 103 213 102 69 930 85 Cuts at: 4495 5566 2661 Size: 1071 3444 Fragments arranged by size: 3444 1071 930 234 213 103 102 98 85 69 BsmI GAATG_Cn' Cuts at: 3860 3953 3860 Size: 93 6256 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2162 2379 2547 2661 2895 2993 3096 Size: 399 372 217 168 114 234 98 103 Cuts at: 3096 3228 3303 3309 3411 3480 3668 4410 4495 Size: 132 75 6 102 69 188 742 85 Cuts at: 4495 5566 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 372 234 217 188 168 132 114 103 102 98 85 75 69 6 BspEI T'CCGG_A Cuts at: 6012 6012 Size: 6349 BspHI T'CATG_A Cuts at: 4238 5968 4238 Size: 1730 4619 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2989 3704 844 Size: 2145 715 3489 Fragments arranged by size: 3489 2145 715 BsrBI CCG'CTC Cuts at: 1394 1823 2853 4236 1394 Size: 429 1030 1383 3507 Fragments arranged by size: 3507 1383 1030 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2128 4844 5018 65 Size: 1183 880 2716 174 1396 Fragments arranged by size: 2716 1396 1183 880 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2079 3188 3209 4993 5180 1700 Size: 110 269 1109 21 1784 187 2869 Fragments arranged by size: 2869 1784 1109 269 187 110 21 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6349 BssHII G'CGCG_C Cuts at: 1925 3205 3235 1925 Size: 1280 30 5039 Fragments arranged by size: 5039 1280 30 BssSI C'ACGA_G Cuts at: 4409 5421 4409 Size: 1012 5337 BstAPI GCAn_nnn'nTGC Cuts at: 794 2890 794 Size: 2096 4253 BstBI TT'CG_AA Cuts at: 5153 5153 Size: 6349 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6349 BstXI CCAn_nnnn'nTGG Cuts at: 2311 2824 3526 2311 Size: 513 702 5134 Fragments arranged by size: 5134 702 513 BstYI r'GATC_y Cuts at: 1972 2673 3438 3679 4046 4431 4448 5900 5986 Size: 701 765 241 367 385 17 1452 86 Cuts at: 5986 5998 6344 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 765 701 385 367 346 241 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3317 3429 3317 Size: 112 6237 BtgI C'CryG_G Cuts at: 513 1065 2004 2397 3219 3375 3519 513 Size: 552 939 393 822 156 144 3343 Fragments arranged by size: 3343 939 822 552 393 156 144 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3354 487 Size: 683 56 2128 3482 Fragments arranged by size: 3482 2128 683 56 BtsI GCAGTG_nn' Cuts at: 2329 3405 3625 3865 4659 4679 2329 Size: 1076 220 240 794 20 3999 Fragments arranged by size: 3999 1076 794 240 220 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6314 DraI TTT'AAA Cuts at: 3653 3653 Size: 6349 DraIII CAC_nnn'GTG Cuts at: 3424 3484 3424 Size: 60 6289 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2837 3536 5356 809 Size: 507 1521 699 1820 1802 Fragments arranged by size: 1820 1802 1521 699 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2255 2346 2740 Size: 54 1425 50 275 18 425 91 394 Cuts at: 2740 2865 3387 3796 4685 6314 8 Size: 125 522 409 889 1629 43 Fragments arranged by size: 1629 1425 889 522 425 409 394 275 125 91 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2255 2740 3796 1537 Size: 275 443 485 1056 4090 Fragments arranged by size: 4090 1056 485 443 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2525 2981 4279 1353 Size: 1172 456 1298 3423 Fragments arranged by size: 3423 1298 1172 456 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2690 4936 5310 5456 387 Size: 1064 138 1101 2246 374 146 1280 Fragments arranged by size: 2246 1280 1101 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2659 3307 2659 Size: 648 5701 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2540 2924 3005 4412 5009 5796 6064 Size: 393 908 384 81 1407 597 787 268 Cuts at: 6064 1239 Size: 1524 Fragments arranged by size: 1524 1407 908 787 597 393 384 268 81 EcoO109I rG'GnC_Cy Cuts at: 2365 2365 Size: 6349 EcoRI G'AATT_C Cuts at: 3658 4036 3658 Size: 378 5971 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2809 2809 Size: 6349 FspI TGC'GCA Cuts at: 3257 4855 3257 Size: 1598 4751 FspAI rTGC'GCAy Cuts at: 3257 3257 Size: 6349 HaeII r_GCGC'y Cuts at: 1678 2170 5496 1678 Size: 492 3326 2531 Fragments arranged by size: 3326 2531 492 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2675 2707 3495 3527 4990 Size: 32 135 32 1103 32 788 32 1463 Cuts at: 4990 5022 5064 5096 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1463 1103 788 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3693 3939 669 Size: 988 281 1755 246 3079 Fragments arranged by size: 3079 1755 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6349 HpaI GTT'AAC Cuts at: 3939 3939 Size: 6349 KpnI G_GTAC'C Cuts at: 3674 3674 Size: 6349 MfeI C'AATT_G Cuts at: 3948 3948 Size: 6349 MluI A'CGCG_T Cuts at: 6225 6225 Size: 6349 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2634 2718 5463 5647 1305 Size: 1329 84 2745 184 2007 Fragments arranged by size: 2745 2007 1329 184 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 2348 2867 10 Size: 54 1425 343 516 519 3492 Fragments arranged by size: 3492 1425 519 516 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2475 2950 3191 3524 4307 4666 4825 Size: 648 1309 475 241 333 783 359 159 Cuts at: 4825 518 Size: 2042 Fragments arranged by size: 2042 1309 783 648 475 359 333 241 159 MspA1I CmG'CkG Cuts at: 1606 2902 2962 3765 4442 5590 5835 1606 Size: 1296 60 803 677 1148 245 2120 Fragments arranged by size: 2120 1296 1148 803 677 245 60 NaeI GCC'GGC Cuts at: 2081 2081 Size: 6349 NcoI C'CATG_G Cuts at: 513 1065 2004 2397 3219 513 Size: 552 939 393 822 3643 Fragments arranged by size: 3643 939 822 552 393 NdeI CA'TA_TG Cuts at: 387 387 Size: 6349 NgoMIV G'CCGG_C Cuts at: 2079 2079 Size: 6349 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6349 NotI GC'GGCC_GC Cuts at: 2255 3796 2255 Size: 1541 4808 NruI TCG'CGA Cuts at: 3045 3045 Size: 6349 NsiI A_TGCA'T Cuts at: 3496 3496 Size: 6349 NspI r_CATG'y Cuts at: 1132 1229 3013 3707 5252 1132 Size: 97 1784 694 1545 2229 Fragments arranged by size: 2229 1784 1545 694 97 PasI CC'CwG_GG Cuts at: 1348 2379 1348 Size: 1031 5318 PciI A'CATG_T Cuts at: 5248 5248 Size: 6349 PflMI CCAn_nnn'nTGG Cuts at: 1108 3569 1108 Size: 2461 3888 PfoI T'CCnGG_A Cuts at: 2661 3411 2661 Size: 750 5599 PmeI GTTT'AAAC Cuts at: 3653 3653 Size: 6349 PmlI CAC'GTG Cuts at: 2890 3349 2890 Size: 459 5890 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4418 4450 5931 5963 4418 Size: 32 1481 32 4804 Fragments arranged by size: 4804 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6349 PsiI TTA'TAA Cuts at: 3919 3919 Size: 6349 PstI C_TGCA'G Cuts at: 830 3701 830 Size: 2871 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6349 PvuII CAG'CTG Cuts at: 2902 2902 Size: 6349 SacI G_AGCT'C Cuts at: 2661 3309 2661 Size: 648 5701 SalI G'TCGA_C Cuts at: 1936 3691 1936 Size: 1755 4594 SapI GCTCTTCn'nnn_ Cuts at: 2981 2981 Size: 6349 SbfI CC_TGCA'GG Cuts at: 3701 3701 Size: 6349 ScaI AGT'ACT Cuts at: 4597 6309 4597 Size: 1712 4637 SfcI C'TryA_G Cuts at: 826 1045 3697 4832 5513 5704 826 Size: 219 2652 1135 681 191 1471 Fragments arranged by size: 2652 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6349 SgrAI Cr'CCGG_yG Cuts at: 1700 3188 3209 1700 Size: 1488 21 4840 Fragments arranged by size: 4840 1488 21 SmaI CCC'GGG Cuts at: 1872 3676 1872 Size: 1804 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2510 4453 5354 5616 820 Size: 197 926 567 1943 901 262 1553 Fragments arranged by size: 1943 1553 926 901 567 262 197 SnaBI TAC'GTA Cuts at: 493 4140 493 Size: 3647 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6349 SphI G_CATG'C Cuts at: 1132 3013 3707 1132 Size: 1881 694 3774 Fragments arranged by size: 3774 1881 694 SspI AAT'ATT Cuts at: 5 52 4273 5 Size: 47 4221 2081 Fragments arranged by size: 4221 2081 47 StuI AGG'CCT Cuts at: 1517 2297 1517 Size: 780 5569 StyI C'CwwG_G Cuts at: 513 1065 2004 2397 3219 3327 3788 513 Size: 552 939 393 822 108 461 3074 Fragments arranged by size: 3074 939 822 552 461 393 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2371 2487 4376 4393 4546 4731 5176 Size: 1255 791 116 1889 17 153 185 445 Cuts at: 5176 325 Size: 1498 Fragments arranged by size: 1889 1498 1255 791 445 185 153 116 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2769 2802 4595 Size: 274 80 33 51 1443 792 33 1793 Cuts at: 4595 6307 96 Size: 1712 138 Fragments arranged by size: 1793 1712 1443 792 274 138 80 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3086 3118 6309 6341 3086 Size: 32 3191 32 3094 Fragments arranged by size: 3191 3094 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3125 3605 1316 Size: 1809 480 4060 Fragments arranged by size: 4060 1809 480 XbaI T'CTAG_A Cuts at: 3685 3685 Size: 6349 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2821 2821 Size: 6349 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6349 XmaI C'CCGG_G Cuts at: 1870 3674 1870 Size: 1804 4545 XmnI GAAnn'nnTTC Cuts at: 4478 4478 Size: 6349 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4157 276 Size: 53 83 186 936 2623 2468 Fragments arranged by size: 2623 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FseI KasI NarI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI