################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI AlwNI Aor51HI ApoI AscI AseI AsnI Asp718I AspEI AvaI AvrII BamHI BbrPI BbvCI BciVI BfaI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsiWI BsmI BsoBI Bsp1407I BspDI BspLU11I BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MseI MunI NaeI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 541 TCCGGA T^CCGGA Alw44I 1500 GTGCAC G^TGCAC AlwI 597 GGATC GGATC(4/5) ApaLI 1500 GTGCAC G^TGCAC AspI 226 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 677 TGCGCA TGC^GCA BalI 1100 TGGCCA TGG^CCA BcgI 993 CGAGCTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1238 TGATCA T^GATCA BglII 1346 AGATCT A^GATCT BsaWI 541 TCCGGA [AT]^CCGG[AT] BseAI 541 TCCGGA T^CCGGA BspEI 541 TCCGGA T^CCGGA BspHI 1121 TCATGA T^CATGA BsrBI 1327 GAGCGG CCGCTC(-3/-3) BsrFI 41 ACCGGC [GA]^CCGG[CT] BsrI 1287 CCAGT ACTGG(1/-1) BssHII 1110 GCGCGC G^CGCGC BssSI 1622 CACGAG CACGAG(-5/-1) BstYI 1346 AGATCT [GA]^GATC[CT] Cfr10I 41 ACCGGC [GA]^CCGG[CT] DraIII 1492 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 943 GACCTGTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 688 GAAGAG CTCTTC(1/4) EcoNI 1080 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1238 TGATCA T^GATCA FspI 677 TGCGCA TGC^GCA Ksp632I 688 GAAGAG CTCTTC(1/4) MflI 1346 AGATCT [GA]^GATC[CT] MluNI 1100 TGGCCA TGG^CCA MroI 541 TCCGGA T^CCGGA MscI 1100 TGGCCA TGG^CCA NcoI 1542 CCATGG C^CATGG NotI 1716 GCGGCCGC GC^GGCCGC NspI 1307 ACATGC [GA]CATG^[CT] PflFI 226 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1389 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 561 CAGCTG CAG^CTG RcaI 1121 TCATGA T^CATGA SapI 688 GAAGAGC GCTCTTC(1/4) SfiI 477 GGCCGTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 1297 CTCAAG C^T[CT][GA]AG TfiI 813 GAATC G^A[AT]TC Tsp509I 535 AATT ^AATT TspRI 1288 CAGTG CA[GC]TG[ACGT][ACGT]^ Tth111I 226 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1389 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1346 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AocI 1612 CCTGAGG CC^T[ACGT]AGG 1669 CCTGAGG BbeI 439 GGCGCC GGCGC^C 1487 GGCGCC BglI 478 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 642 GCCTCTATGGC BsaI 829 GAGACC GGTCTC(1/5) 1018 GGTCTC BsiEI 476 CGGCCG CG[GA][CT]^CG 1717 CGGCCG BsmBI 704 CGTCTC CGTCTC(1/5) 656 GAGACG BsmFI 33 GTCCC GGGAC(10/14) 1218 GTCCC BstAPI 45 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1427 GCAAGGTGTGC BstXI 1037 CCAGCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1091 CCACCGCGGTGG Bsu36I 1612 CCTGAGG CC^T[ACGT]AGG 1669 CCTGAGG EagI 476 CGGCCG C^GGCCG 1717 CGGCCG EclXI 476 CGGCCG C^GGCCG 1717 CGGCCG Eco52I 476 CGGCCG C^GGCCG 1717 CGGCCG Eco81I 1612 CCTGAGG CC^T[ACGT]AGG 1669 CCTGAGG FauI 54 GCGGG CCCGC(4/6) 1352 GCGGG KasI 439 GGCGCC G^GCGCC 1487 GGCGCC MslI 306 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1092 CACCGCGGTG NarI 439 GGCGCC GG^CGCC 1487 GGCGCC PpuMI 1607 AGGACCC [GA]G^G[AT]CC[CT] 1616 AGGACCC PstI 334 CTGCAG CTGCA^G 1266 CTGCAG SacI 58 GAGCTC GAGCT^C 994 GAGCTC SfcI 334 CTGCAG C^T[GA][CT]AG 1266 CTGCAG SfoI 439 GGCGCC GGC^GCC 1487 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AatII AccII AciI AcyI AfaI AluI ApaI Asp700I AspHI AvaII BanI BanII BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KspI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI RsaI SacII Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TthHB8I XmnI