(Circular) (Six-base) MAPSORT of: pFN21AE4154.seq Check: 1814 from: 1 to: 6472 pFN21AE4154 6472 bp With 182 enzymes: SgfI * March 28, 2012 13:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2061 2848 2061 Size: 787 5685 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2103 2204 2912 4282 Size: 53 83 186 936 567 101 708 1370 Cuts at: 4282 278 Size: 2468 Fragments arranged by size: 2468 1370 936 708 567 186 101 83 53 AccI GT'mk_AC Cuts at: 1937 3815 1937 Size: 1878 4594 Acc65I G'GTAC_C Cuts at: 3793 3793 Size: 6472 AclI AA'CG_TT Cuts at: 1563 4599 4972 1563 Size: 3036 373 3063 Fragments arranged by size: 3063 3036 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3173 4535 5919 6187 1632 Size: 1541 1362 1384 268 1917 Fragments arranged by size: 1917 1541 1384 1362 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6275 AflIII A'CryG_T Cuts at: 1203 5371 6348 1203 Size: 4168 977 1327 Fragments arranged by size: 4168 1327 977 AgeI A'CCGG_T Cuts at: 5303 5303 Size: 6472 AhdI GACnn_n'nnGTC Cuts at: 5201 5201 Size: 6472 AleI CACnn'nnGTG Cuts at: 1166 3101 1166 Size: 1935 4537 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6472 AlwNI CAG_nnn'CTG Cuts at: 4215 5787 4215 Size: 1572 4900 ApaI G_GGCC'C Cuts at: 3056 3214 3540 3603 3056 Size: 158 326 63 5925 Fragments arranged by size: 5925 326 158 63 ApaLI G'TGCA_C Cuts at: 3505 4529 5685 3505 Size: 1024 1156 4292 Fragments arranged by size: 4292 1156 1024 ApoI r'AATT_y Cuts at: 1493 3781 4007 4159 6268 1493 Size: 2288 226 152 2109 1697 Fragments arranged by size: 2288 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5026 160 Size: 872 3994 1606 Fragments arranged by size: 3994 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 AvaI C'yCGr_G Cuts at: 1870 1943 3797 1870 Size: 73 1854 4545 Fragments arranged by size: 4545 1854 73 BamHI G'GATC_C Cuts at: 3802 4169 3802 Size: 367 6105 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2049 2444 2890 3492 3793 Size: 325 217 627 262 395 446 602 301 Cuts at: 3793 5248 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 602 446 395 325 301 262 217 BanII G_rGCy'C Cuts at: 1391 2067 2613 3003 3056 3214 3540 3572 3603 Size: 676 546 390 53 158 326 32 31 Cuts at: 3603 3791 1391 Size: 188 4072 Fragments arranged by size: 4072 676 546 390 326 188 158 53 32 31 BbeI G_GCGC'C Cuts at: 2448 3496 2448 Size: 1048 5424 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2119 2204 3442 3717 928 Size: 971 220 85 1238 275 3683 Fragments arranged by size: 3683 1238 971 275 220 85 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2987 3021 4663 4697 1903 Size: 34 1050 34 1642 34 3678 Fragments arranged by size: 3678 1642 1050 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4364 5574 4364 Size: 1210 5262 BclI T'GATC_A Cuts at: 1548 3243 1548 Size: 1695 4777 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2489 2653 5083 Size: 107 122 71 631 605 817 164 2430 Cuts at: 5083 136 Size: 1525 Fragments arranged by size: 2430 1525 817 631 605 164 122 107 71 BglII A'GATC_T Cuts at: 3351 6467 3351 Size: 3116 3356 BlpI GC'TnA_GC Cuts at: 3889 3889 Size: 6472 Bme1580I G_kGCm'C Cuts at: 1790 3056 3214 3509 3540 3603 4533 5689 1790 Size: 1266 158 295 31 63 930 1156 2573 Fragments arranged by size: 2573 1266 1156 930 295 158 63 31 BmgBI CAC'GTC Cuts at: 1318 2665 3163 1318 Size: 1347 498 4627 Fragments arranged by size: 4627 1347 498 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4203 5161 442 Size: 416 53 83 399 24 2786 958 1753 Fragments arranged by size: 2786 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6472 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3730 3730 Size: 6472 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2679 3472 3558 3629 5132 1239 Size: 1440 793 86 71 1503 2579 Fragments arranged by size: 2579 1503 1440 793 86 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3287 4597 5462 5760 3287 Size: 1310 865 298 3999 Fragments arranged by size: 3999 1310 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2828 3029 5135 882 Size: 617 1329 201 2106 2219 Fragments arranged by size: 2219 2106 1329 617 201 BsaAI yAC'GTr Cuts at: 493 1204 4263 493 Size: 711 3059 2702 Fragments arranged by size: 3059 2702 711 BsaBI GATnn'nnATC Cuts at: 6466 6466 Size: 6472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2088 2100 2201 2412 Size: 53 83 186 936 555 12 101 211 Cuts at: 2412 2445 2909 3081 3493 4279 4661 5296 275 Size: 33 464 172 412 786 382 635 1451 Fragments arranged by size: 1451 936 786 635 555 464 412 382 211 186 172 101 83 53 33 12 BsaWI w'CCGG_w Cuts at: 2546 4905 5303 5577 5724 6135 2546 Size: 2359 398 274 147 411 2883 Fragments arranged by size: 2883 2359 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6247 Fragments arranged by size: 6247 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2512 3000 3032 3048 3656 1186 Size: 1326 488 32 16 608 4002 Fragments arranged by size: 4002 1326 608 488 32 16 BseYI C'CCAG_C Cuts at: 2152 3166 3646 5675 6171 2152 Size: 1014 480 2029 496 2453 Fragments arranged by size: 2453 2029 1014 496 480 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2213 2772 3460 1163 Size: 573 477 559 688 4175 Fragments arranged by size: 4175 688 573 559 477 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2484 3725 3922 4683 5711 1540 Size: 275 186 483 1241 197 761 1028 2301 Fragments arranged by size: 2301 1241 1028 761 483 275 197 186 BsiHKAI G_wGCw'C Cuts at: 2067 3003 3384 3509 4533 4618 5689 2067 Size: 936 381 125 1024 85 1071 2850 Fragments arranged by size: 2850 1071 1024 936 381 125 85 BsmI GAATG_Cn' Cuts at: 3983 4076 3983 Size: 93 6379 BsmBI CGTCTCn'nnnn_ Cuts at: 2655 2715 2655 Size: 60 6412 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2613 3003 3056 3214 3384 3509 Size: 399 277 546 390 53 158 170 125 Cuts at: 3509 3540 3572 3603 3791 4533 4618 5689 1391 Size: 31 32 31 188 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 546 399 390 277 188 170 158 125 85 53 32 31 31 BspEI T'CCGG_A Cuts at: 2546 6135 2546 Size: 3589 2883 BspHI T'CATG_A Cuts at: 3126 4361 6091 3126 Size: 1235 1730 3507 Fragments arranged by size: 3507 1730 1235 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2061 2689 2848 3120 3207 3827 844 Size: 1217 628 159 272 87 620 3489 Fragments arranged by size: 3489 1217 628 620 272 159 87 BsrBI CCG'CTC Cuts at: 1394 1823 3334 4359 1394 Size: 429 1511 1025 3507 Fragments arranged by size: 3507 1511 1025 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4967 5141 65 Size: 1183 3719 174 1396 Fragments arranged by size: 3719 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2046 5116 5303 1700 Size: 110 236 3070 187 2869 Fragments arranged by size: 3070 2869 236 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6472 BssHII G'CGCG_C Cuts at: 1925 3115 1925 Size: 1190 5282 BssSI C'ACGA_G Cuts at: 3627 4532 5544 3627 Size: 905 1012 4555 Fragments arranged by size: 4555 1012 905 BstAPI GCAn_nnn'nTGC Cuts at: 794 2056 3438 794 Size: 1262 1382 3828 Fragments arranged by size: 3828 1382 1262 BstBI TT'CG_AA Cuts at: 5276 5276 Size: 6472 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6472 BstXI CCAn_nnnn'nTGG Cuts at: 3049 3103 3049 Size: 54 6418 BstYI r'GATC_y Cuts at: 1972 3351 3802 4169 4554 4571 6023 6109 6121 Size: 1379 451 367 385 17 1452 86 12 Cuts at: 6121 6467 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1379 451 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3618 3675 3618 Size: 57 6415 BtgI C'CryG_G Cuts at: 513 1065 2214 2510 2802 3099 3496 3523 3547 Size: 552 1149 296 292 297 397 27 24 Cuts at: 3547 3719 513 Size: 172 3266 Fragments arranged by size: 3266 1149 552 397 297 296 292 172 27 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2545 487 Size: 683 56 1319 4414 Fragments arranged by size: 4414 1319 683 56 BtsI GCAGTG_nn' Cuts at: 3988 4782 4802 3988 Size: 794 20 5658 Fragments arranged by size: 5658 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6472 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6437 DraI TTT'AAA Cuts at: 3776 3776 Size: 6472 DraIII CAC_nnn'GTG Cuts at: 3502 3502 Size: 6472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2954 5479 809 Size: 507 1638 2525 1802 Fragments arranged by size: 2525 1802 1638 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2481 2490 2556 Size: 54 1425 50 275 18 651 9 66 Cuts at: 2556 2918 3105 3282 3520 3531 3722 3919 4808 Size: 362 187 177 238 11 191 197 889 Cuts at: 4808 6437 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 651 362 275 238 197 191 187 177 66 54 50 43 18 11 9 EagI C'GGCC_G Cuts at: 1537 1812 2481 3722 3919 1537 Size: 275 669 1241 197 4090 Fragments arranged by size: 4090 1241 669 275 197 EarI CTCTTCn'nnn_ Cuts at: 1353 2688 4402 1353 Size: 1335 1714 3423 Fragments arranged by size: 3423 1714 1335 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3448 3570 3653 3707 5059 5433 Size: 1064 138 1859 122 83 54 1352 374 Cuts at: 5433 5579 387 Size: 146 1280 Fragments arranged by size: 1859 1352 1280 1064 374 146 138 122 83 54 EcoICRI GAG'CTC Cuts at: 2065 3001 2065 Size: 936 5536 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2679 3173 3472 3558 3629 4535 5132 Size: 393 1047 494 299 86 71 906 597 Cuts at: 5132 5919 6187 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1047 906 787 597 494 393 299 268 86 71 EcoNI CCTnn'n_nnAGG Cuts at: 3089 3089 Size: 6472 EcoO109I rG'GnC_Cy Cuts at: 3053 3210 3537 3600 3613 3622 3664 3743 3053 Size: 157 327 63 13 9 42 79 5782 Fragments arranged by size: 5782 327 157 79 63 42 13 9 EcoRI G'AATT_C Cuts at: 3781 4159 3781 Size: 378 6094 FspI TGC'GCA Cuts at: 2684 4978 2684 Size: 2294 4178 HaeII r_GCGC'y Cuts at: 1678 2294 2390 2448 3125 3394 3496 3584 5619 Size: 616 96 58 677 269 102 88 2035 Cuts at: 5619 1678 Size: 2531 Fragments arranged by size: 2531 2035 677 616 269 102 96 88 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2306 2338 3164 3196 5113 Size: 32 135 32 734 32 826 32 1917 Cuts at: 5113 5145 5187 5219 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1917 826 734 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3816 4062 669 Size: 988 281 1878 246 3079 Fragments arranged by size: 3079 1878 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6472 HpaI GTT'AAC Cuts at: 4062 4062 Size: 6472 KasI G'GCGC_C Cuts at: 2444 3492 2444 Size: 1048 5424 KpnI G_GTAC'C Cuts at: 3797 3797 Size: 6472 MfeI C'AATT_G Cuts at: 4071 4071 Size: 6472 MluI A'CGCG_T Cuts at: 6348 6348 Size: 6472 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2184 2388 3000 5586 5770 1305 Size: 879 204 612 2586 184 2007 Fragments arranged by size: 2586 2007 879 612 204 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3107 10 Size: 54 1425 343 1275 3375 Fragments arranged by size: 3375 1425 1275 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 3101 4430 4789 4948 518 Size: 648 1149 786 1329 359 159 2042 Fragments arranged by size: 2042 1329 1149 786 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2568 3101 3525 3721 3888 4565 5713 5958 Size: 962 533 424 196 167 677 1148 245 Cuts at: 5958 1606 Size: 2120 Fragments arranged by size: 2120 1148 962 677 533 424 245 196 167 NarI GG'CG_CC Cuts at: 2445 3493 2445 Size: 1048 5424 NcoI C'CATG_G Cuts at: 513 1065 3547 513 Size: 552 2482 3438 Fragments arranged by size: 3438 2482 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6472 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6472 NotI GC'GGCC_GC Cuts at: 3722 3919 3722 Size: 197 6275 NspI r_CATG'y Cuts at: 1132 1229 3316 3830 5375 1132 Size: 97 2087 514 1545 2229 Fragments arranged by size: 2229 2087 1545 514 97 PasI CC'CwG_GG Cuts at: 1348 2245 1348 Size: 897 5575 PciI A'CATG_T Cuts at: 5371 5371 Size: 6472 PflMI CCAn_nnn'nTGG Cuts at: 1108 3400 1108 Size: 2292 4180 PmeI GTTT'AAAC Cuts at: 3776 3776 Size: 6472 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2753 2785 3569 3601 4541 4573 6054 6086 2753 Size: 32 784 32 940 32 1481 32 3139 Fragments arranged by size: 3139 1481 940 784 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3613 3622 3613 Size: 9 6463 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6472 PsiI TTA'TAA Cuts at: 4042 4042 Size: 6472 PspOMI G'GGCC_C Cuts at: 3052 3210 3536 3599 3052 Size: 158 326 63 5925 Fragments arranged by size: 5925 326 158 63 PstI C_TGCA'G Cuts at: 830 2343 3275 3824 830 Size: 1513 932 549 3478 Fragments arranged by size: 3478 1513 932 549 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6472 PvuII CAG'CTG Cuts at: 2568 2568 Size: 6472 SacI G_AGCT'C Cuts at: 2067 3003 2067 Size: 936 5536 SacII CC_GC'GG Cuts at: 3102 3526 3722 3102 Size: 424 196 5852 Fragments arranged by size: 5852 424 196 SalI G'TCGA_C Cuts at: 1936 3814 1936 Size: 1878 4594 SapI GCTCTTCn'nnn_ Cuts at: 2688 2688 Size: 6472 SbfI CC_TGCA'GG Cuts at: 3824 3824 Size: 6472 ScaI AGT'ACT Cuts at: 4720 6432 4720 Size: 1712 4760 SfcI C'TryA_G Cuts at: 826 1045 2339 3271 3820 4955 5636 5827 826 Size: 219 1294 932 549 1135 681 191 1471 Fragments arranged by size: 1471 1294 1135 932 681 549 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2489 2489 Size: 6472 SfoI GGC'GCC Cuts at: 2446 3494 2446 Size: 1048 5424 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6472 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6472 SmaI CCC'GGG Cuts at: 1872 3799 1872 Size: 1927 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3302 4576 5477 5739 820 Size: 197 926 1359 1274 901 262 1553 Fragments arranged by size: 1553 1359 1274 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4263 493 Size: 3770 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6472 SphI G_CATG'C Cuts at: 1132 3830 1132 Size: 2698 3774 SspI AAT'ATT Cuts at: 5 52 4396 5 Size: 47 4344 2081 Fragments arranged by size: 4344 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6472 StyI C'CwwG_G Cuts at: 513 1065 2613 2742 3547 3911 513 Size: 552 1548 129 805 364 3074 Fragments arranged by size: 3074 1548 805 552 364 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4499 4516 4669 4854 5299 325 Size: 1255 2919 17 153 185 445 1498 Fragments arranged by size: 2919 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2274 2755 4718 Size: 274 80 33 51 1443 297 481 1963 Cuts at: 4718 6430 96 Size: 1712 138 Fragments arranged by size: 1963 1712 1443 481 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3206 3238 6432 6464 3206 Size: 32 3194 32 3214 Fragments arranged by size: 3214 3194 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2234 1316 Size: 918 5554 XbaI T'CTAG_A Cuts at: 3808 3808 Size: 6472 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6472 XmaI C'CCGG_G Cuts at: 1870 3797 1870 Size: 1927 4545 XmnI GAAnn'nnTTC Cuts at: 2765 3233 3254 3365 4601 2765 Size: 468 21 111 1236 4636 Fragments arranged by size: 4636 1236 468 111 21 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2101 2202 2910 4280 Size: 53 83 186 936 567 101 708 1370 Cuts at: 4280 276 Size: 2468 Fragments arranged by size: 2468 1370 936 708 567 186 101 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SanDI SexAI SrfI SwaI XcmI