(Linear) (Six-base) MAPSORT of: KIEE4154.seq Check: 9918 from: 1 to: 1767 KIEE4154.orf With 182 enzymes: SgfI * March 22, 2012 17:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 56 843 1767 Size: 56 787 924 Fragments arranged by size: 924 787 56 AatII G_ACGT'C Cuts at: 0 98 199 907 1767 Size: 98 101 708 860 Fragments arranged by size: 860 708 101 98 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1168 1767 Size: 1168 599 AleI CACnn'nnGTG Cuts at: 0 1096 1767 Size: 1096 671 ApaI G_GGCC'C Cuts at: 0 1051 1209 1535 1598 1767 Size: 1051 158 326 63 169 Fragments arranged by size: 1051 326 169 158 63 ApaLI G'TGCA_C Cuts at: 0 1500 1767 Size: 1500 267 BanI G'GyrC_C Cuts at: 0 44 439 885 1487 1767 Size: 44 395 446 602 280 Fragments arranged by size: 602 446 395 280 44 BanII G_rGCy'C Cuts at: 0 62 608 998 1051 1209 1535 1567 1598 Size: 62 546 390 53 158 326 32 31 Cuts at: 1598 1767 Size: 169 Fragments arranged by size: 546 390 326 169 158 62 53 32 31 BbeI G_GCGC'C Cuts at: 0 443 1491 1767 Size: 443 1048 276 Fragments arranged by size: 1048 443 276 BbsI GAAGACnn'nnnn_ Cuts at: 0 114 199 1437 1712 1767 Size: 114 85 1238 275 55 Fragments arranged by size: 1238 275 114 85 55 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 982 1016 1767 Size: 982 34 751 Fragments arranged by size: 982 751 34 BclI T'GATC_A Cuts at: 0 1238 1767 Size: 1238 529 BglI GCCn_nnn'nGGC Cuts at: 0 484 648 1767 Size: 484 164 1119 Fragments arranged by size: 1119 484 164 BglII A'GATC_T Cuts at: 0 1346 1767 Size: 1346 421 Bme1580I G_kGCm'C Cuts at: 0 1051 1209 1504 1535 1598 1767 Size: 1051 158 295 31 63 169 Fragments arranged by size: 1051 295 169 158 63 31 BmgBI CAC'GTC Cuts at: 0 660 1158 1767 Size: 660 498 609 Fragments arranged by size: 660 609 498 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1725 1767 Size: 1725 42 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 674 1467 1553 1624 1767 Size: 674 793 86 71 143 Fragments arranged by size: 793 674 143 86 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1282 1767 Size: 1282 485 BsaI GGTCTCn'nnnn_ Cuts at: 0 823 1024 1767 Size: 823 201 743 Fragments arranged by size: 823 743 201 BsaHI Gr'CG_yC Cuts at: 0 83 95 196 407 440 904 1076 1488 Size: 83 12 101 211 33 464 172 412 Cuts at: 1488 1767 Size: 279 Fragments arranged by size: 464 412 279 211 172 101 83 33 12 BsaWI w'CCGG_w Cuts at: 0 541 1767 Size: 541 1226 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 507 995 1027 1043 1651 1767 Size: 507 488 32 16 608 116 Fragments arranged by size: 608 507 488 116 32 16 BseYI C'CCAG_C Cuts at: 0 147 1161 1641 1767 Size: 147 1014 480 126 Fragments arranged by size: 1014 480 147 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 767 1455 1767 Size: 208 559 688 312 Fragments arranged by size: 688 559 312 208 BsiEI CG_ry'CG Cuts at: 0 479 1720 1767 Size: 479 1241 47 Fragments arranged by size: 1241 479 47 BsiHKAI G_wGCw'C Cuts at: 0 62 998 1379 1504 1767 Size: 62 936 381 125 263 Fragments arranged by size: 936 381 263 125 62 BsmBI CGTCTCn'nnnn_ Cuts at: 0 650 710 1767 Size: 650 60 1057 Fragments arranged by size: 1057 650 60 Bsp1286I G_dGCh'C Cuts at: 0 62 608 998 1051 1209 1379 1504 1535 Size: 62 546 390 53 158 170 125 31 Cuts at: 1535 1567 1598 1767 Size: 32 31 169 Fragments arranged by size: 546 390 170 169 158 125 62 53 32 31 31 BspEI T'CCGG_A Cuts at: 0 541 1767 Size: 541 1226 BspHI T'CATG_A Cuts at: 0 1121 1767 Size: 1121 646 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 56 684 843 1115 1202 1767 Size: 56 628 159 272 87 565 Fragments arranged by size: 628 565 272 159 87 56 BsrBI CCG'CTC Cuts at: 0 1329 1767 Size: 1329 438 BsrFI r'CCGG_y Cuts at: 0 41 1767 Size: 41 1726 BssHII G'CGCG_C Cuts at: 0 1110 1767 Size: 1110 657 BssSI C'ACGA_G Cuts at: 0 1622 1767 Size: 1622 145 BstAPI GCAn_nnn'nTGC Cuts at: 0 51 1433 1767 Size: 51 1382 334 Fragments arranged by size: 1382 334 51 BstXI CCAn_nnnn'nTGG Cuts at: 0 1044 1098 1767 Size: 1044 54 669 Fragments arranged by size: 1044 669 54 BstYI r'GATC_y Cuts at: 0 1346 1767 Size: 1346 421 Bsu36I CC'TnA_GG Cuts at: 0 1613 1670 1767 Size: 1613 57 97 Fragments arranged by size: 1613 97 57 BtgI C'CryG_G Cuts at: 0 209 505 797 1094 1491 1518 1542 1714 Size: 209 296 292 297 397 27 24 172 Cuts at: 1714 1767 Size: 53 Fragments arranged by size: 397 297 296 292 209 172 53 27 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 540 1767 Size: 540 1227 DraIII CAC_nnn'GTG Cuts at: 0 1497 1767 Size: 1497 270 DrdI GACnn_nn'nnGTC Cuts at: 0 949 1767 Size: 949 818 EaeI y'GGCC_r Cuts at: 0 476 485 551 913 1100 1277 1515 1526 Size: 476 9 66 362 187 177 238 11 Cuts at: 1526 1717 1767 Size: 191 50 Fragments arranged by size: 476 362 238 191 187 177 66 50 11 9 EagI C'GGCC_G Cuts at: 0 476 1717 1767 Size: 476 1241 50 Fragments arranged by size: 1241 476 50 EarI CTCTTCn'nnn_ Cuts at: 0 683 1767 Size: 683 1084 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1443 1565 1648 1702 1767 Size: 1443 122 83 54 65 Fragments arranged by size: 1443 122 83 65 54 EcoICRI GAG'CTC Cuts at: 0 60 996 1767 Size: 60 936 771 Fragments arranged by size: 936 771 60 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 674 1168 1467 1553 1624 1767 Size: 674 494 299 86 71 143 Fragments arranged by size: 674 494 299 143 86 71 EcoNI CCTnn'n_nnAGG Cuts at: 0 1084 1767 Size: 1084 683 EcoO109I rG'GnC_Cy Cuts at: 0 1048 1205 1532 1595 1608 1617 1659 1738 Size: 1048 157 327 63 13 9 42 79 Cuts at: 1738 1767 Size: 29 Fragments arranged by size: 1048 327 157 79 63 42 29 13 9 FspI TGC'GCA Cuts at: 0 679 1767 Size: 679 1088 HaeII r_GCGC'y Cuts at: 0 289 385 443 1120 1389 1491 1579 1767 Size: 289 96 58 677 269 102 88 188 Fragments arranged by size: 677 289 269 188 102 96 88 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 301 333 1159 1191 1767 Size: 301 32 826 32 576 Fragments arranged by size: 826 576 301 32 32 KasI G'GCGC_C Cuts at: 0 439 1487 1767 Size: 439 1048 280 Fragments arranged by size: 1048 439 280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 179 383 995 1767 Size: 179 204 612 772 Fragments arranged by size: 772 612 204 179 MscI TGG'CCA Cuts at: 0 1102 1767 Size: 1102 665 MslI CAynn'nnrTG Cuts at: 0 310 1096 1767 Size: 310 786 671 Fragments arranged by size: 786 671 310 MspA1I CmG'CkG Cuts at: 0 563 1096 1520 1716 1767 Size: 563 533 424 196 51 Fragments arranged by size: 563 533 424 196 51 NarI GG'CG_CC Cuts at: 0 440 1488 1767 Size: 440 1048 279 Fragments arranged by size: 1048 440 279 NcoI C'CATG_G Cuts at: 0 1542 1767 Size: 1542 225 NotI GC'GGCC_GC Cuts at: 0 1717 1767 Size: 1717 50 NspI r_CATG'y Cuts at: 0 1311 1767 Size: 1311 456 PasI CC'CwG_GG Cuts at: 0 240 1767 Size: 240 1527 PflMI CCAn_nnn'nTGG Cuts at: 0 1395 1767 Size: 1395 372 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 748 780 1564 1596 1767 Size: 748 32 784 32 171 Fragments arranged by size: 784 748 171 32 32 PpuMI rG'GwC_Cy Cuts at: 0 1608 1617 1767 Size: 1608 9 150 Fragments arranged by size: 1608 150 9 PspOMI G'GGCC_C Cuts at: 0 1047 1205 1531 1594 1767 Size: 1047 158 326 63 173 Fragments arranged by size: 1047 326 173 158 63 PstI C_TGCA'G Cuts at: 0 338 1270 1767 Size: 338 932 497 Fragments arranged by size: 932 497 338 PvuII CAG'CTG Cuts at: 0 563 1767 Size: 563 1204 SacI G_AGCT'C Cuts at: 0 62 998 1767 Size: 62 936 769 Fragments arranged by size: 936 769 62 SacII CC_GC'GG Cuts at: 0 1097 1521 1717 1767 Size: 1097 424 196 50 Fragments arranged by size: 1097 424 196 50 SapI GCTCTTCn'nnn_ Cuts at: 0 683 1767 Size: 683 1084 SfcI C'TryA_G Cuts at: 0 334 1266 1767 Size: 334 932 501 Fragments arranged by size: 932 501 334 SfiI GGCCn_nnn'nGGCC Cuts at: 0 484 1767 Size: 484 1283 SfoI GGC'GCC Cuts at: 0 441 1489 1767 Size: 441 1048 278 Fragments arranged by size: 1048 441 278 SmlI C'TyrA_G Cuts at: 0 1297 1767 Size: 1297 470 StyI C'CwwG_G Cuts at: 0 608 737 1542 1767 Size: 608 129 805 225 Fragments arranged by size: 805 608 225 129 TatI w'GTAC_w Cuts at: 0 269 750 1767 Size: 269 481 1017 Fragments arranged by size: 1017 481 269 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1201 1233 1767 Size: 1201 32 534 Fragments arranged by size: 1201 534 32 Tth111I GACn'n_nGTC Cuts at: 0 229 1767 Size: 229 1538 XmnI GAAnn'nnTTC Cuts at: 0 760 1228 1249 1360 1767 Size: 760 468 21 111 407 Fragments arranged by size: 760 468 407 111 21 ZraI GAC'GTC Cuts at: 0 96 197 905 1767 Size: 96 101 708 862 Fragments arranged by size: 862 708 101 96 Enzymes that do cut: AarI AatII AcuI AleI ApaI ApaLI BanI BanII BbeI BbsI BcgI BclI BglI BglII Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI NotI NspI PasI PflMI PpiI PpuMI PspOMI PstI PvuII SacI SacII SapI SfcI SfiI SfoI SmlI StyI TatI TstI Tth111I XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI AlwNI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbvCI BciVI BfrBI BlpI BmrI BmtI Bpu10I BsaAI BsaBI BsaXI BsiWI BsmI BsrDI BsrGI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NruI NsiI PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XbaI XcmI XhoI XmaI