################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI Alw44I ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspI AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraIII EarI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HpaI Ksp632I KspI MaeI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp509I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 392 GGTACC G^GTACC AflIII 336 ACACGT A^C[GA][CT]GT AhdI 1091 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 1138 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 888 CCTCAGG CC^T[ACGT]AGG Aor51HI 902 AGCGCT AGC^GCT Asp718I 392 GGTACC G^GTACC AspEI 1091 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 658 GAGCTC G[AT]GC[AT]^C AvaI 171 CCCGGG C^[CT]CG[GA]G BalI 587 TGGCCA TGG^CCA BglI 90 GCCCAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiHKAI 658 GAGCTC G[AT]GC[AT]^C BsoBI 171 CCCGGG C^[CT]CG[GA]G BspMI 6 ACCTGC ACCTGC(4/8) BsrBI 1207 CCGCTC CCGCTC(-3/-3) BsrDI 540 CATTGC GCAATG(2/0) BsrI 981 ACTGG ACTGG(1/-1) BstYI 930 GGATCT [GA]^GATC[CT] Bsu36I 888 CCTCAGG CC^T[ACGT]AGG DraI 969 TTTAAA TTT^AAA DrdI 355 GACCAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 138 CGGCCG C^GGCCG Eam1105I 1091 GACAGGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 138 CGGCCG C^GGCCG Eco47III 902 AGCGCT AGC^GCT Eco52I 138 CGGCCG C^GGCCG Eco81I 888 CCTCAGG CC^T[ACGT]AGG HgaI 1168 GACGC GACGC(5/10) KpnI 392 GGTACC GGTAC^C MaeIII 610 GTGAC ^GT[ACGT]AC MflI 930 GGATCT [GA]^GATC[CT] MluNI 587 TGGCCA TGG^CCA MscI 587 TGGCCA TGG^CCA MseI 970 TTAA T^TAA MslI 480 CACGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 95 GCCGGC GCC^GGC NgoMIV 95 GCCGGC G^CCGGC NlaIII 74 CATG CATG^ PleI 954 GACTC GAGTC(4/5) PpuMI 467 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 1138 CAGCTG CAG^CTG SacI 658 GAGCTC GAGCT^C SmaI 171 CCCGGG CCC^GGG TaqI 899 TCGA T^CGA TfiI 692 GATTC G^A[AT]TC Tru9I 970 TTAA T^TAA Tsp45I 610 GTGAC ^GT[GC]AC TthHB8I 899 TCGA T^CGA XhoII 930 GGATCT [GA]^GATC[CT] XmaCI 171 CCCGGG C^CCGGG XmaI 171 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanII 283 GAGCCC G[GA]GC[CT]^C 658 GAGCTC BbeI 448 GGCGCC GGCGC^C 853 GGCGCC BsiEI 134 CGGTCG CG[GA][CT]^CG 138 CGGCCG BsrFI 95 GCCGGC [GA]^CCGG[CT] 1120 ACCGGC BstF5I 462 CATCC GGATG(2/0) 834 CATCC Cfr10I 95 GCCGGC [GA]^CCGG[CT] 1120 ACCGGC EcoNI 307 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 884 CCTTCCTCAGG FokI 462 CATCC GGATG(9/13) 834 CATCC HinfI 692 GATTC G^A[ACGT]TC 954 GACTC HphI 486 GGTGA GGTGA(8/7) 767 TCACC KasI 448 GGCGCC G^GCGCC 853 GGCGCC MaeII 338 ACGT A^CGT 785 ACGT MspA1I 109 CAGCGG C[AC]G^C[GT]G 1138 CAGCTG NarI 448 GGCGCC GG^CGCC 853 GGCGCC SfaNI 159 GCATC GCATC(5/9) 842 GCATC SfoI 448 GGCGCC GGC^GCC 853 GGCGCC SmlI 661 CTCAAG C^T[CT][GA]AG 956 CTCAAG XcmI 396 CCAGGGTTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 740 CCAGACCCGCCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AvaII BanI BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HincII HindII HpaII ItaI MboI MboII MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI TseI TspRI