(Circular) (Six-base) MAPSORT of: pFN21AE2599.seq Check: 6328 from: 1 to: 5923 pFN21AE2599 5923 bp With 182 enzymes: SgfI * January 6, 2016 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2020 2020 Size: 5923 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 2397 3244 2397 Size: 847 5076 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2111 2801 3075 3179 3986 5370 5638 1632 Size: 479 690 274 104 807 1384 268 1917 Fragments arranged by size: 1917 1384 807 690 479 274 268 104 AfeI AGC'GCT Cuts at: 2909 2909 Size: 5923 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 2341 4822 5799 1203 Size: 1138 2481 977 1327 Fragments arranged by size: 2481 1327 1138 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 3101 4652 3101 Size: 1551 4372 AleI CACnn'nnGTG Cuts at: 1166 2489 1166 Size: 1323 4600 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AlwNI CAG_nnn'CTG Cuts at: 3148 3666 5238 3148 Size: 518 1572 3833 Fragments arranged by size: 3833 1572 518 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 3232 3458 3610 5719 1493 Size: 1739 226 152 2109 1697 Fragments arranged by size: 2109 1739 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2176 3248 1870 Size: 73 233 1072 4545 Fragments arranged by size: 4545 1072 233 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2389 2422 2389 Size: 33 5890 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2397 2453 2858 3244 Size: 325 217 627 221 389 56 405 386 Cuts at: 3244 4699 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 405 389 386 325 221 217 56 BanII G_rGCy'C Cuts at: 1391 2292 2667 3242 1391 Size: 901 375 575 4072 Fragments arranged by size: 4072 901 575 375 BbeI G_GCGC'C Cuts at: 2457 2862 2457 Size: 405 5518 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4952 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2101 4534 136 Size: 107 122 71 631 605 429 2433 1525 Fragments arranged by size: 2433 1525 631 605 429 122 107 71 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 3340 3340 Size: 5923 Bme1580I G_kGCm'C Cuts at: 1790 2486 2611 3984 5140 1790 Size: 696 125 1373 1156 2573 Fragments arranged by size: 2573 1373 1156 696 125 BmgBI CAC'GTC Cuts at: 1318 2344 2791 1318 Size: 1026 447 4450 Fragments arranged by size: 4450 1026 447 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3654 4612 442 Size: 416 53 83 399 24 2237 958 1753 Fragments arranged by size: 2237 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2681 3008 3158 4583 1239 Size: 1442 327 150 1425 2579 Fragments arranged by size: 2579 1442 1425 327 150 Bpu10I CC'TnA_GC Cuts at: 2949 3215 2949 Size: 266 5657 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2651 2946 4048 4913 5211 2651 Size: 295 1102 865 298 3363 Fragments arranged by size: 3363 1102 865 298 295 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4586 882 Size: 617 3087 2219 Fragments arranged by size: 3087 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2454 2859 3174 3730 Size: 53 83 186 936 921 405 315 556 Cuts at: 3730 4112 4747 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 921 635 556 405 382 315 186 83 53 BsaWI w'CCGG_w Cuts at: 4356 4754 5028 5175 5586 4356 Size: 398 274 147 411 4693 Fragments arranged by size: 4693 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2493 2523 1178 Size: 30 165 30 1090 30 4578 Fragments arranged by size: 4578 1090 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5923 BseYI C'CCAG_C Cuts at: 2096 2264 2554 3075 3193 5126 5622 2096 Size: 168 290 521 118 1933 496 2397 Fragments arranged by size: 2397 1933 521 496 290 168 118 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5350 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2142 2146 3373 4134 5162 1540 Size: 275 186 141 4 1227 761 1028 2301 Fragments arranged by size: 2301 1227 1028 761 275 186 141 4 BsiHKAI G_wGCw'C Cuts at: 2667 3984 4069 5140 2667 Size: 1317 85 1071 3450 Fragments arranged by size: 3450 1317 1071 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2292 2486 2611 2667 3242 3984 4069 Size: 399 502 194 125 56 575 742 85 Cuts at: 4069 5140 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 575 502 399 194 125 85 56 BspEI T'CCGG_A Cuts at: 5586 5586 Size: 5923 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2020 3278 844 Size: 1176 1258 3489 Fragments arranged by size: 3489 1258 1176 BsrBI CCG'CTC Cuts at: 1394 1823 3214 3810 1394 Size: 429 1391 596 3507 Fragments arranged by size: 3507 1391 596 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2544 4418 4592 65 Size: 1183 1296 1874 174 1396 Fragments arranged by size: 1874 1396 1296 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2100 3125 4567 4754 1700 Size: 110 290 1025 1442 187 2869 Fragments arranged by size: 2869 1442 1025 290 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5923 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstYI r'GATC_y Cuts at: 1972 2935 3253 3620 4005 4022 5474 5560 5572 Size: 963 318 367 385 17 1452 86 12 Cuts at: 5572 5918 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 963 385 367 346 318 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2894 2894 Size: 5923 BtgI C'CryG_G Cuts at: 513 1065 2004 2478 2754 2954 513 Size: 552 939 474 276 200 3482 Fragments arranged by size: 3482 939 552 474 276 200 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 2455 2720 2835 3439 4233 4253 2455 Size: 265 115 604 794 20 4125 Fragments arranged by size: 4125 794 604 265 115 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 2976 3227 2976 Size: 251 5672 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2366 4930 809 Size: 507 1050 2564 1802 Fragments arranged by size: 2564 1802 1050 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2143 2514 2592 Size: 54 1425 50 275 18 313 371 78 Cuts at: 2592 2832 3370 4259 5888 8 Size: 240 538 889 1629 43 Fragments arranged by size: 1629 1425 889 538 371 313 275 240 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2143 3370 1537 Size: 275 331 1227 4090 Fragments arranged by size: 4090 1227 331 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3853 1353 Size: 2500 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2875 4510 4884 5030 387 Size: 1064 138 1286 1635 374 146 1280 Fragments arranged by size: 1635 1286 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2665 2665 Size: 5923 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2681 2801 3008 3075 3158 3179 Size: 393 479 570 120 207 67 83 21 Cuts at: 3179 3986 4583 5370 5638 1239 Size: 807 597 787 268 1524 Fragments arranged by size: 1524 807 787 597 570 479 393 268 207 120 83 67 21 EcoNI CCTnn'n_nnAGG Cuts at: 2316 2893 2316 Size: 577 5346 EcoO109I rG'GnC_Cy Cuts at: 2309 2473 3004 3018 2309 Size: 164 531 14 5214 Fragments arranged by size: 5214 531 164 14 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FspI TGC'GCA Cuts at: 4429 4429 Size: 5923 HaeII r_GCGC'y Cuts at: 1678 2265 2457 2862 2911 5070 1678 Size: 587 192 405 49 2159 2531 Fragments arranged by size: 2531 2159 587 405 192 49 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2226 2258 3087 3119 4564 Size: 32 135 32 654 32 829 32 1445 Cuts at: 4564 4596 4638 4670 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1445 829 654 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2359 2968 3123 3267 3513 669 Size: 988 281 421 609 155 144 246 3079 Fragments arranged by size: 3079 988 609 421 281 246 155 144 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KasI G'GCGC_C Cuts at: 2453 2858 2453 Size: 405 5518 KpnI G_GTAC'C Cuts at: 2401 3248 2401 Size: 847 5076 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5037 5221 1305 Size: 3732 184 2007 Fragments arranged by size: 3732 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2594 10 Size: 54 1425 343 762 3339 Fragments arranged by size: 3339 1425 762 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2489 3881 4240 4399 518 Size: 648 1323 1392 359 159 2042 Fragments arranged by size: 2042 1392 1323 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2116 3145 3339 4016 5164 5409 1606 Size: 510 1029 194 677 1148 245 2120 Fragments arranged by size: 2120 1148 1029 677 510 245 194 NaeI GCC'GGC Cuts at: 2102 2102 Size: 5923 NarI GG'CG_CC Cuts at: 2454 2859 2454 Size: 405 5518 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4432 Fragments arranged by size: 4432 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NgoMIV G'CCGG_C Cuts at: 2100 2100 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 3370 3370 Size: 5923 NspI r_CATG'y Cuts at: 1132 1229 3281 4826 1132 Size: 97 2052 1545 2229 Fragments arranged by size: 2229 2052 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5923 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5923 PfoI T'CCnGG_A Cuts at: 3081 3081 Size: 5923 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3992 4024 5505 5537 3992 Size: 32 1481 32 4378 Fragments arranged by size: 4378 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2473 2473 Size: 5923 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 3145 3145 Size: 5923 SacI G_AGCT'C Cuts at: 2667 2667 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SanDI GG'GwC_CC Cuts at: 2473 2473 Size: 5923 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 4171 5883 4171 Size: 1712 4211 SfcI C'TryA_G Cuts at: 826 1045 3271 4406 5087 5278 826 Size: 219 2226 1135 681 191 1471 Fragments arranged by size: 2226 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2455 2860 2455 Size: 405 5518 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 2178 3250 1872 Size: 306 1072 4545 Fragments arranged by size: 4545 1072 306 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 2961 4027 4928 5190 820 Size: 197 926 723 295 1066 901 262 1553 Fragments arranged by size: 1553 1066 926 901 723 295 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2004 3362 513 Size: 552 939 1358 3074 Fragments arranged by size: 3074 1358 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2128 2374 3950 3967 4120 4305 4750 Size: 1255 548 246 1576 17 153 185 445 Cuts at: 4750 325 Size: 1498 Fragments arranged by size: 1576 1498 1255 548 445 246 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2541 4169 5881 Size: 274 80 33 51 1443 564 1628 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 1712 1628 1443 564 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5883 5915 5883 Size: 32 5891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2408 2752 2408 Size: 344 5579 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 2176 3248 1870 Size: 306 1072 4545 Fragments arranged by size: 4545 1072 306 XmnI GAAnn'nnTTC Cuts at: 4052 4052 Size: 5923 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI