################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BamHI BanI BbeI BbrPI BcgI BciVI BfrI BglI BglII BlnI BsaBI BseAI BsiEI BsiWI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FseI FspI HincII HindII HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SalI SbfI ScaI SfcI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcyI 100 GACGCC G[GA]^CG[CT]C AlwNI 575 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 279 AGCGCT AGC^GCT Asp700I 1257 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 1249 GAAGAC GAAGAC(2/6) BbvCI 515 CCTCAGC CCTCAGC(-5/-2) BfaI 1246 CTAG C^TAG BlpI 1332 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1332 GCTGAGC GC^T[ACGT]AGC BpuAI 1249 GAAGAC GAAGAC(2/6) BsaAI 1510 TACGTG [CT]AC^GT[GA] BsaHI 100 GACGCC G[GA]^CG[CT]C BsaWI 1253 ACCGGA [AT]^CCGG[AT] BsgI 889 CTGCAC GTGCAG(16/14) BsmBI 433 GAGACG CGTCTC(1/5) BsmFI 481 GGGAC GGGAC(10/14) Bsp1407I 402 TGTACA T^GTACA BspMI 1344 ACCTGC ACCTGC(4/8) BsrGI 402 TGTACA T^GTACA BssSI 31 CTCGTG CACGAG(-5/-1) BstAPI 368 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 170 TTCGAA TT^CGAA BstXI 653 CCAGACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1332 GCTGAGC GC^T[ACGT]AGC DraII 390 AGGACCC [GA]G^G[ACGT]CC[CT] Eco47III 279 AGCGCT AGC^GCT EcoO109I 390 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT22I 1640 ATGCAT ATGCA^T Hin1I 100 GACGCC G[GA]^CG[CT]C KspI 95 CCGCGG CCGC^GG MaeI 1246 CTAG C^TAG MslI 1184 CACGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 298 GCCGGC GCC^GGC NgoMIV 298 GCCGGC G^CCGGC NsiI 1640 ATGCAT ATGCA^T NspI 1638 ACATGC [GA]CATG^[CT] NspV 170 TTCGAA TT^CGAA PpuMI 390 AGGACCC [GA]G^G[AT]CC[CT] PvuII 518 CAGCTG CAG^CTG SacII 95 CCGCGG CCGC^GG SapI 1354 GAAGAGC GCTCTTC(1/4) SexAI 488 ACCAGGT A^CC[AT]GGT SfuI 170 TTCGAA TT^CGAA SspBI 402 TGTACA T^GTACA XcmI 615 CCAAGCCTTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1257 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcsI 1104 AAATTC [GA]^AATT[CT] 1487 AAATTC ApoI 1104 AAATTC [GA]^AATT[CT] 1487 AAATTC AspI 270 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 487 GACCAGGTC BclI 1097 TGATCA T^GATCA 1392 TGATCA DrdI 58 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1563 GACGAGACTGTC EcoT14I 470 CCTTGG C^C[AT][AT]GG 716 CCAAGG FauI 39 GCGGG CCCGC(4/6) 1002 GCGGG FbaI 1097 TGATCA T^GATCA 1392 TGATCA HindIII 1318 AAGCTT A^AGCTT 1444 AAGCTT MaeIII 56 GTGAC ^GT[ACGT]AC 745 GTCAC MspA1I 95 CCGCGG C[AC]G^C[GT]G 518 CAGCTG NlaIII 780 CATG CATG^ 1639 CATG PflFI 270 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 487 GACCAGGTC StyI 470 CCTTGG C^C[AT][AT]GG 716 CCAAGG Tsp45I 56 GTGAC ^GT[GC]AC 745 GTCAC Tth111I 270 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC 487 GACCAGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 82 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaII BalI BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MboI MboII MflI MluNI MnlI MscI MseI MspI MvaI MvnI MwoI NciI NdeII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII