(Circular) (Six-base) MAPSORT of: pFN21AE1983.seq Check: 8272 from: 1 to: 6382 pFN21AE1983 6382 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4192 278 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3725 1937 Size: 1788 4594 Acc65I G'GTAC_C Cuts at: 3703 3703 Size: 6382 AclI AA'CG_TT Cuts at: 1563 4509 4882 1563 Size: 2946 373 3063 Fragments arranged by size: 3063 2946 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2288 2433 4445 5829 6097 1632 Size: 656 145 2012 1384 268 1917 Fragments arranged by size: 2012 1917 1384 656 268 145 AfeI AGC'GCT Cuts at: 2286 2286 Size: 6382 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6185 AflIII A'CryG_T Cuts at: 1203 5281 6258 1203 Size: 4078 977 1327 Fragments arranged by size: 4078 1327 977 AgeI A'CCGG_T Cuts at: 5213 5213 Size: 6382 AhdI GACnn_n'nnGTC Cuts at: 5111 5111 Size: 6382 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6382 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2612 1184 Size: 1428 4954 AlwNI CAG_nnn'CTG Cuts at: 2585 4125 5697 2585 Size: 1540 1572 3270 Fragments arranged by size: 3270 1572 1540 ApaLI G'TGCA_C Cuts at: 4439 5595 4439 Size: 1156 5226 ApoI r'AATT_y Cuts at: 1493 3109 3492 3691 3917 4069 6178 1493 Size: 1616 383 199 226 152 2109 1697 Fragments arranged by size: 2109 1697 1616 383 226 199 152 AseI AT'TA_AT Cuts at: 160 1032 4936 160 Size: 872 3904 1606 Fragments arranged by size: 3904 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 AvaI C'yCGr_G Cuts at: 1870 1943 3707 1870 Size: 73 1764 4545 Fragments arranged by size: 4545 1764 73 BamHI G'GATC_C Cuts at: 3712 4079 3712 Size: 367 6015 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3703 5158 618 Size: 325 217 627 1916 1455 1842 Fragments arranged by size: 1916 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2328 2616 3210 3701 1391 Size: 937 288 594 491 4072 Fragments arranged by size: 4072 937 594 491 288 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3261 928 Size: 971 1362 4049 Fragments arranged by size: 4049 1362 971 BbvCI CC'TCA_GC Cuts at: 2521 2521 Size: 6382 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4573 4607 1903 Size: 34 2636 34 3678 Fragments arranged by size: 3678 2636 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4274 5484 4274 Size: 1210 5172 BclI T'GATC_A Cuts at: 1548 3102 3397 1548 Size: 1554 295 4533 Fragments arranged by size: 4533 1554 295 BfrBI ATG'CAT Cuts at: 3647 3647 Size: 6382 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4993 136 Size: 107 122 71 631 605 3321 1525 Fragments arranged by size: 3321 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6377 6377 Size: 6382 BlpI GC'TnA_GC Cuts at: 3338 3799 3338 Size: 461 5921 Bme1580I G_kGCm'C Cuts at: 1790 4443 5599 1790 Size: 2653 1156 2573 Fragments arranged by size: 2653 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6382 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2493 4113 5071 Size: 416 53 83 399 24 1076 1620 958 Cuts at: 5071 442 Size: 1753 Fragments arranged by size: 1753 1620 1076 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6382 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2342 2342 Size: 6382 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2309 2366 2687 2764 3386 5042 1239 Size: 1070 57 321 77 622 1656 2579 Fragments arranged by size: 2579 1656 1070 622 321 77 57 Bpu10I CC'TnA_GC Cuts at: 2521 2626 3072 3224 2521 Size: 105 446 152 5679 Fragments arranged by size: 5679 446 152 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4507 5372 5670 4507 Size: 865 298 5219 Fragments arranged by size: 5219 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2170 2503 2841 2961 3454 5045 882 Size: 617 671 333 338 120 493 1591 2219 Fragments arranged by size: 2219 1591 671 617 493 338 333 120 BsaAI yAC'GTr Cuts at: 493 1204 3517 4173 493 Size: 711 2313 656 2702 Fragments arranged by size: 2702 2313 711 656 BsaBI GATnn'nnATC Cuts at: 6376 6376 Size: 6382 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2106 4189 4571 5206 Size: 53 83 186 936 573 2083 382 635 Cuts at: 5206 275 Size: 1451 Fragments arranged by size: 2083 1451 936 635 573 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3258 4815 5213 5487 5634 6045 3258 Size: 1557 398 274 147 411 3595 Fragments arranged by size: 3595 1557 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2483 2513 2821 2851 1178 Size: 30 165 30 1080 30 308 30 4709 Fragments arranged by size: 4709 1080 308 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2074 2086 2206 2336 2903 3038 3098 3164 Size: 888 12 120 130 567 135 60 66 Cuts at: 3164 1186 Size: 4404 Fragments arranged by size: 4404 888 567 135 130 120 66 60 12 BseYI C'CCAG_C Cuts at: 2066 2460 2630 2690 5585 6081 2066 Size: 394 170 60 2895 496 2367 Fragments arranged by size: 2895 2367 496 394 170 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2879 1163 Size: 573 1143 4666 Fragments arranged by size: 4666 1143 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3832 4593 5621 1540 Size: 275 186 1831 761 1028 2301 Fragments arranged by size: 2301 1831 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 2616 3210 4443 4528 5599 2328 Size: 288 594 1233 85 1071 3111 Fragments arranged by size: 3111 1233 1071 594 288 85 BsmI GAATG_Cn' Cuts at: 3893 3986 3893 Size: 93 6289 BsmBI CGTCTCn'nnnn_ Cuts at: 2432 2432 Size: 6382 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2616 3210 3701 4443 4528 5599 Size: 399 538 288 594 491 742 85 1071 Cuts at: 5599 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 594 538 491 399 288 85 BspEI T'CCGG_A Cuts at: 6045 6045 Size: 6382 BspHI T'CATG_A Cuts at: 4271 6001 4271 Size: 1730 4652 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3358 3737 844 Size: 2514 379 3489 Fragments arranged by size: 3489 2514 379 BsrBI CCG'CTC Cuts at: 1394 1823 2028 2052 3007 4269 1394 Size: 429 205 24 955 1262 3507 Fragments arranged by size: 3507 1262 955 429 205 24 BsrDI GCAATG_nn' Cuts at: 65 1248 4877 5051 65 Size: 1183 3629 174 1396 Fragments arranged by size: 3629 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2303 2859 3231 5026 5213 1700 Size: 110 493 556 372 1795 187 2869 Fragments arranged by size: 2869 1795 556 493 372 187 110 BsrGI T'GTAC_A Cuts at: 96 2407 96 Size: 2311 4071 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6382 BssSI C'ACGA_G Cuts at: 2036 4442 5454 2036 Size: 2406 1012 2964 Fragments arranged by size: 2964 2406 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2379 794 Size: 1585 4797 BstBI TT'CG_AA Cuts at: 2176 5186 2176 Size: 3010 3372 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6382 BstXI CCAn_nnnn'nTGG Cuts at: 2665 2665 Size: 6382 BstYI r'GATC_y Cuts at: 1972 2223 2672 3300 3712 4079 4464 4481 5933 Size: 251 449 628 412 367 385 17 1452 Cuts at: 5933 6019 6031 6377 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 628 449 412 385 367 346 251 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2100 2676 2829 2850 3188 3354 Size: 552 939 96 576 153 21 338 166 Cuts at: 3354 513 Size: 3541 Fragments arranged by size: 3541 939 576 552 338 166 153 96 21 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5643 Fragments arranged by size: 5643 683 56 BtsI GCAGTG_nn' Cuts at: 2699 3182 3898 4692 4712 2699 Size: 483 716 794 20 4369 Fragments arranged by size: 4369 794 716 483 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6382 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6347 DraI TTT'AAA Cuts at: 3686 3686 Size: 6382 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2069 3574 5389 809 Size: 507 753 1505 1815 1802 Fragments arranged by size: 1815 1802 1505 753 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2088 2208 Size: 54 1425 50 275 18 177 81 120 Cuts at: 2208 2301 2478 2907 3213 3233 3829 4718 6347 Size: 93 177 429 306 20 596 889 1629 Cuts at: 6347 8 Size: 43 Fragments arranged by size: 1629 1425 889 596 429 306 275 177 177 120 93 81 54 50 43 20 18 EagI C'GGCC_G Cuts at: 1537 1812 3829 1537 Size: 275 2017 4090 Fragments arranged by size: 4090 2017 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2774 2783 2871 2935 3354 3415 4312 1353 Size: 1421 9 88 64 419 61 897 3423 Fragments arranged by size: 3423 1421 897 419 88 64 61 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2703 4969 5343 5489 387 Size: 1064 138 1114 2266 374 146 1280 Fragments arranged by size: 2266 1280 1114 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2326 2614 3208 2326 Size: 288 594 5500 Fragments arranged by size: 5500 594 288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2288 2309 2366 2433 2687 2764 3386 Size: 393 656 21 57 67 254 77 622 Cuts at: 3386 4445 5042 5829 6097 1239 Size: 1059 597 787 268 1524 Fragments arranged by size: 1524 1059 787 656 622 597 393 268 254 77 67 57 21 EcoO109I rG'GnC_Cy Cuts at: 2396 2396 Size: 6382 EcoRI G'AATT_C Cuts at: 3691 4069 3691 Size: 378 6004 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2394 2394 Size: 6382 FspI TGC'GCA Cuts at: 4888 4888 Size: 6382 HaeII r_GCGC'y Cuts at: 1678 2288 2906 3158 5529 1678 Size: 610 618 252 2371 2531 Fragments arranged by size: 2531 2371 618 610 252 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2164 2196 2483 2515 3285 Size: 32 135 32 592 32 287 32 770 Cuts at: 3285 3317 5023 5055 5097 5129 1373 Size: 32 1706 32 42 32 2626 Fragments arranged by size: 2626 1706 770 592 287 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3726 3972 669 Size: 988 281 1788 246 3079 Fragments arranged by size: 3079 1788 988 281 246 HindIII A'AGCT_T Cuts at: 748 3323 3449 748 Size: 2575 126 3681 Fragments arranged by size: 3681 2575 126 HpaI GTT'AAC Cuts at: 3972 3972 Size: 6382 KpnI G_GTAC'C Cuts at: 3707 3707 Size: 6382 MfeI C'AATT_G Cuts at: 3981 3981 Size: 6382 MluI A'CGCG_T Cuts at: 6258 6258 Size: 6382 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1995 2415 5496 5680 1305 Size: 690 420 3081 184 2007 Fragments arranged by size: 3081 2007 690 420 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 2480 2909 10 Size: 54 1425 343 378 270 429 3483 Fragments arranged by size: 3483 1425 429 378 343 270 54 MslI CAynn'nnrTG Cuts at: 518 1166 3193 4340 4699 4858 518 Size: 648 2027 1147 359 159 2042 Fragments arranged by size: 2042 2027 1147 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2102 2525 3798 4475 5623 5868 1606 Size: 496 423 1273 677 1148 245 2120 Fragments arranged by size: 2120 1273 1148 677 496 423 245 NaeI GCC'GGC Cuts at: 2305 2305 Size: 6382 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4891 Fragments arranged by size: 4891 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6382 NgoMIV G'CCGG_C Cuts at: 2303 2303 Size: 6382 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6382 NotI GC'GGCC_GC Cuts at: 3829 3829 Size: 6382 NsiI A_TGCA'T Cuts at: 3649 3649 Size: 6382 NspI r_CATG'y Cuts at: 1132 1229 3647 3740 5285 1132 Size: 97 2418 93 1545 2229 Fragments arranged by size: 2418 2229 1545 97 93 PasI CC'CwG_GG Cuts at: 1348 2215 1348 Size: 867 5515 PciI A'CATG_T Cuts at: 5281 5281 Size: 6382 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6382 PmeI GTTT'AAAC Cuts at: 3686 3686 Size: 6382 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4451 4483 5964 5996 4451 Size: 32 1481 32 4837 Fragments arranged by size: 4837 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2396 2396 Size: 6382 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6382 PsiI TTA'TAA Cuts at: 3952 3952 Size: 6382 PstI C_TGCA'G Cuts at: 830 3734 830 Size: 2904 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6382 PvuII CAG'CTG Cuts at: 2525 2525 Size: 6382 SacI G_AGCT'C Cuts at: 2328 2616 3210 2328 Size: 288 594 5500 Fragments arranged by size: 5500 594 288 SacII CC_GC'GG Cuts at: 2103 2103 Size: 6382 SalI G'TCGA_C Cuts at: 1936 3724 1936 Size: 1788 4594 SapI GCTCTTCn'nnn_ Cuts at: 3354 3354 Size: 6382 SbfI CC_TGCA'GG Cuts at: 3734 3734 Size: 6382 ScaI AGT'ACT Cuts at: 4630 6342 4630 Size: 1712 4670 SexAI A'CCwGG_T Cuts at: 2493 2493 Size: 6382 SfcI C'TryA_G Cuts at: 826 1045 3730 4865 5546 5737 826 Size: 219 2685 1135 681 191 1471 Fragments arranged by size: 2685 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6382 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6382 SmaI CCC'GGG Cuts at: 1872 3709 1872 Size: 1837 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4486 5387 5649 820 Size: 197 926 2543 901 262 1553 Fragments arranged by size: 2543 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4173 493 Size: 3680 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6382 SphI G_CATG'C Cuts at: 1132 3740 1132 Size: 2608 3774 SspI AAT'ATT Cuts at: 5 52 4306 5 Size: 47 4254 2081 Fragments arranged by size: 4254 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6382 StyI C'CwwG_G Cuts at: 513 1065 2004 2475 2721 3821 513 Size: 552 939 471 246 1100 3074 Fragments arranged by size: 3074 1100 939 552 471 246 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2437 2734 2842 4409 4426 4579 4764 Size: 1255 857 297 108 1567 17 153 185 Cuts at: 4764 5209 325 Size: 445 1498 Fragments arranged by size: 1567 1498 1255 857 445 297 185 153 108 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2407 3618 4628 Size: 274 80 33 51 1443 430 1211 1010 Cuts at: 4628 6340 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1211 1010 430 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2821 2853 6342 6374 2821 Size: 32 3489 32 2829 Fragments arranged by size: 3489 2829 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2278 2495 1316 Size: 962 217 5203 Fragments arranged by size: 5203 962 217 XbaI T'CTAG_A Cuts at: 3718 3718 Size: 6382 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2627 2627 Size: 6382 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6382 XmaI C'CCGG_G Cuts at: 1870 3707 1870 Size: 1837 4545 XmnI GAAnn'nnTTC Cuts at: 3266 4511 3266 Size: 1245 5137 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4190 276 Size: 53 83 186 936 2656 2468 Fragments arranged by size: 2656 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI