(Linear) (Six-base) MAPSORT of: KIEE1983.seq Check: 1513 from: 1 to: 1677 KIEE1983.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 428 1677 Size: 283 145 1249 Fragments arranged by size: 1249 283 145 AfeI AGC'GCT Cuts at: 0 281 1677 Size: 281 1396 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 607 1677 Size: 607 1070 AlwNI CAG_nnn'CTG Cuts at: 0 580 1677 Size: 580 1097 ApoI r'AATT_y Cuts at: 0 1104 1487 1677 Size: 1104 383 190 Fragments arranged by size: 1104 383 190 BanII G_rGCy'C Cuts at: 0 323 611 1205 1677 Size: 323 288 594 472 Fragments arranged by size: 594 472 323 288 BbsI GAAGACnn'nnnn_ Cuts at: 0 1256 1677 Size: 1256 421 BbvCI CC'TCA_GC Cuts at: 0 516 1677 Size: 516 1161 BclI T'GATC_A Cuts at: 0 1097 1392 1677 Size: 1097 295 285 Fragments arranged by size: 1097 295 285 BfrBI ATG'CAT Cuts at: 0 1642 1677 Size: 1642 35 BlpI GC'TnA_GC Cuts at: 0 1333 1677 Size: 1333 344 BmrI ACTGGGnnnn_n' Cuts at: 0 488 1677 Size: 488 1189 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 337 1677 Size: 337 1340 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 361 682 759 1381 1677 Size: 304 57 321 77 622 296 Fragments arranged by size: 622 321 304 296 77 57 Bpu10I CC'TnA_GC Cuts at: 0 516 621 1067 1219 1677 Size: 516 105 446 152 458 Fragments arranged by size: 516 458 446 152 105 BsaI GGTCTCn'nnnn_ Cuts at: 0 165 498 836 956 1449 1677 Size: 165 333 338 120 493 228 Fragments arranged by size: 493 338 333 228 165 120 BsaAI yAC'GTr Cuts at: 0 1512 1677 Size: 1512 165 BsaHI Gr'CG_yC Cuts at: 0 101 1677 Size: 101 1576 BsaWI w'CCGG_w Cuts at: 0 1253 1677 Size: 1253 424 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 478 508 816 846 1677 Size: 478 30 308 30 831 Fragments arranged by size: 831 478 308 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 69 81 201 331 898 1033 1093 1159 Size: 69 12 120 130 567 135 60 66 Cuts at: 1159 1677 Size: 518 Fragments arranged by size: 567 518 135 130 120 69 66 60 12 BseYI C'CCAG_C Cuts at: 0 61 455 625 685 1677 Size: 61 394 170 60 992 Fragments arranged by size: 992 394 170 61 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 874 1677 Size: 874 803 BsiHKAI G_wGCw'C Cuts at: 0 323 611 1205 1677 Size: 323 288 594 472 Fragments arranged by size: 594 472 323 288 BsmBI CGTCTCn'nnnn_ Cuts at: 0 427 1677 Size: 427 1250 Bsp1286I G_dGCh'C Cuts at: 0 323 611 1205 1677 Size: 323 288 594 472 Fragments arranged by size: 594 472 323 288 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1353 1677 Size: 1353 324 BsrBI CCG'CTC Cuts at: 0 23 47 1002 1677 Size: 23 24 955 675 Fragments arranged by size: 955 675 24 23 BsrFI r'CCGG_y Cuts at: 0 298 854 1226 1677 Size: 298 556 372 451 Fragments arranged by size: 556 451 372 298 BsrGI T'GTAC_A Cuts at: 0 402 1677 Size: 402 1275 BssSI C'ACGA_G Cuts at: 0 31 1677 Size: 31 1646 BstAPI GCAn_nnn'nTGC Cuts at: 0 374 1677 Size: 374 1303 BstBI TT'CG_AA Cuts at: 0 171 1677 Size: 171 1506 BstXI CCAn_nnnn'nTGG Cuts at: 0 660 1677 Size: 660 1017 BstYI r'GATC_y Cuts at: 0 218 667 1295 1677 Size: 218 449 628 382 Fragments arranged by size: 628 449 382 218 BtgI C'CryG_G Cuts at: 0 95 671 824 845 1183 1349 1677 Size: 95 576 153 21 338 166 328 Fragments arranged by size: 576 338 328 166 153 95 21 BtsI GCAGTG_nn' Cuts at: 0 694 1177 1677 Size: 694 483 500 Fragments arranged by size: 694 500 483 DrdI GACnn_nn'nnGTC Cuts at: 0 64 1569 1677 Size: 64 1505 108 Fragments arranged by size: 1505 108 64 EaeI y'GGCC_r Cuts at: 0 2 83 203 296 473 902 1208 1228 Size: 2 81 120 93 177 429 306 20 Cuts at: 1228 1677 Size: 449 Fragments arranged by size: 449 429 306 177 120 93 81 20 2 EarI CTCTTCn'nnn_ Cuts at: 0 769 778 866 930 1349 1410 1677 Size: 769 9 88 64 419 61 267 Fragments arranged by size: 769 419 267 88 64 61 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 698 1677 Size: 698 979 EcoICRI GAG'CTC Cuts at: 0 321 609 1203 1677 Size: 321 288 594 474 Fragments arranged by size: 594 474 321 288 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 283 304 361 428 682 759 1381 1677 Size: 283 21 57 67 254 77 622 296 Fragments arranged by size: 622 296 283 254 77 67 57 21 EcoO109I rG'GnC_Cy Cuts at: 0 391 1677 Size: 391 1286 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 389 1677 Size: 389 1288 HaeII r_GCGC'y Cuts at: 0 283 901 1153 1677 Size: 283 618 252 524 Fragments arranged by size: 618 524 283 252 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 159 191 478 510 1280 1312 1677 Size: 159 32 287 32 770 32 365 Fragments arranged by size: 770 365 287 159 32 32 32 HindIII A'AGCT_T Cuts at: 0 1318 1444 1677 Size: 1318 126 233 Fragments arranged by size: 1318 233 126 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 410 1677 Size: 410 1267 MscI TGG'CCA Cuts at: 0 205 475 904 1677 Size: 205 270 429 773 Fragments arranged by size: 773 429 270 205 MslI CAynn'nnrTG Cuts at: 0 1188 1677 Size: 1188 489 MspA1I CmG'CkG Cuts at: 0 97 520 1677 Size: 97 423 1157 Fragments arranged by size: 1157 423 97 NaeI GCC'GGC Cuts at: 0 300 1677 Size: 300 1377 NgoMIV G'CCGG_C Cuts at: 0 298 1677 Size: 298 1379 NsiI A_TGCA'T Cuts at: 0 1644 1677 Size: 1644 33 NspI r_CATG'y Cuts at: 0 1642 1677 Size: 1642 35 PasI CC'CwG_GG Cuts at: 0 210 1677 Size: 210 1467 PpuMI rG'GwC_Cy Cuts at: 0 391 1677 Size: 391 1286 PvuII CAG'CTG Cuts at: 0 520 1677 Size: 520 1157 SacI G_AGCT'C Cuts at: 0 323 611 1205 1677 Size: 323 288 594 472 Fragments arranged by size: 594 472 323 288 SacII CC_GC'GG Cuts at: 0 98 1677 Size: 98 1579 SapI GCTCTTCn'nnn_ Cuts at: 0 1349 1677 Size: 1349 328 SexAI A'CCwGG_T Cuts at: 0 488 1677 Size: 488 1189 StyI C'CwwG_G Cuts at: 0 470 716 1677 Size: 470 246 961 Fragments arranged by size: 961 470 246 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 432 729 837 1677 Size: 432 297 108 840 Fragments arranged by size: 840 432 297 108 TatI w'GTAC_w Cuts at: 0 402 1613 1677 Size: 402 1211 64 Fragments arranged by size: 1211 402 64 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 816 848 1677 Size: 816 32 829 Fragments arranged by size: 829 816 32 Tth111I GACn'n_nGTC Cuts at: 0 273 490 1677 Size: 273 217 1187 Fragments arranged by size: 1187 273 217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 622 1677 Size: 622 1055 XmnI GAAnn'nnTTC Cuts at: 0 1261 1677 Size: 1261 416 Enzymes that do cut: AcuI AfeI AlfI AlwNI ApoI BanII BbsI BbvCI BclI BfrBI BlpI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HindIII MmeI MscI MslI MspA1I NaeI NgoMIV NsiI NspI PasI PpuMI PvuII SacI SacII SapI SexAI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI BciVI BglI BglII Bme1580I BmgBI BmtI BpuEI BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrDI BssHII BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI NarI NcoI NdeI NheI NotI NruI PacI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI XmaI ZraI