################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## Acc65I AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbsI BbvCI BciVI BclI BfaI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HincII HindII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 532 GACGTC GACGT^C AccI 322 GTCTAC GT^[AC][GT]AC AccII 91 CGCG CG^CG AcyI 532 GACGTC G[GA]^CG[CT]C AlwNI 282 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 260 GGGCCC GGGCC^C AspHI 268 GAGCAC G[AT]GC[AT]^C BanI 372 GGTGCC G^G[CT][GA]CC BbrPI 291 CACGTG CAC^GTG BglI 253 GCCGAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 121 AGATCT A^GATCT BsaAI 291 CACGTG [CT]AC^GT[GA] BsaHI 532 GACGTC G[GA]^CG[CT]C BseRI 163 GAGGAG GAGGAG(10/8) BsiEI 251 CGGCCG CG[GA][CT]^CG BsiHKAI 268 GAGCAC G[AT]GC[AT]^C BspMI 679 ACCTGC ACCTGC(4/8) BsrI 69 CCAGT ACTGG(1/-1) BstUI 91 CGCG CG^CG BstYI 121 AGATCT [GA]^GATC[CT] EagI 251 CGGCCG C^GGCCG EclXI 251 CGGCCG C^GGCCG Eco52I 251 CGGCCG C^GGCCG EcoNI 693 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 284 GGCGCT [GA]GCGC^[CT] Hin1I 532 GACGTC G[GA]^CG[CT]C HindIII 402 AAGCTT A^AGCTT HphI 299 TCACC GGTGA(8/7) MaeIII 37 GTGAC ^GT[ACGT]AC MflI 121 AGATCT [GA]^GATC[CT] MspA1I 687 CCGCTG C[AC]G^C[GT]G MvnI 91 CGCG CG^CG NlaIII 645 CATG CATG^ PleI 659 GACTC GAGTC(4/5) PmaCI 291 CACGTG CAC^GTG PmlI 291 CACGTG CAC^GTG SfaNI 79 GATGC GCATC(5/9) SfiI 252 GGCCGAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 500 AGGCCT AGG^CCT Tsp45I 37 GTGAC ^GT[GC]AC Tsp509I 544 AATT ^AATT XhoII 121 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwI 278 GATCC GGATC(4/5) 350 GGATC AvaII 217 GGTCC G^G[AT]CC 600 GGACC BcgI 203 CGACAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 653 GCAGTGGACTCG BsgI 288 CTGCAC GTGCAG(16/14) 508 CTGCAC BsmAI 142 GAGAC GTCTC(1/5) 166 GAGAC BstF5I 634 CATCC GGATG(2/0) 78 GGATG DdeI 125 CTGAG C^T[ACGT]AG 266 CTGAG EaeI 251 CGGCCG [CT]^GGCC[GA] 460 CGGCCA EarI 139 GAAGAG CTCTTC(1/4) 574 GAAGAG FauI 686 CCCGC CCCGC(4/6) 424 GCGGG FokI 634 CATCC GGATG(9/13) 78 GGATG Ksp632I 139 GAAGAG CTCTTC(1/4) 574 GAAGAG MaeII 292 ACGT A^CGT 533 ACGT MboII 139 GAAGA GAAGA(8/7) 574 GAAGA PflMI 220 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 522 CCAAACTCTGG TfiI 53 GATTC G^A[AT]TC 441 GAATC Van91I 220 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 522 CCAAACTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AluI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DpnII DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MnlI MspI MvaI MwoI NciI NdeII NlaIV PstI Sau3AI Sau96I ScrFI SfcI TseI TspRI