################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI AocI AscI AseI AsnI Asp718I AspEI AvrII BamHI BanI BbeI BbrPI BfrI BglI BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiEI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI Hin1I HindIII KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TfiI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccII 1827 CGCG CG^CG AflIII 1329 ACGTGT A^C[GA][CT]GT Alw44I 253 GTGCAC G^TGCAC Aor51HI 1360 AGCGCT AGC^GCT ApaI 373 GGGCCC GGGCC^C ApaLI 253 GTGCAC G^TGCAC AspHI 253 GTGCAC G[AT]GC[AT]^C AspI 172 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1232 GGACC G^G[AT]CC AviII 1340 TGCGCA TGC^GCA BbvCI 1849 GCTGAGG CCTCAGC(-5/-2) BcgI 1839 CGAAGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 410 GTATCC GTATCC(6/5) BclI 536 TGATCA T^GATCA BcnI 377 CCCGG CC^[GC]GG BglII 14 AGATCT A^GATCT BlpI 297 GCTGAGC GC^T[ACGT]AGC Bpu1102I 297 GCTGAGC GC^T[ACGT]AGC BsaAI 1328 TACGTG [CT]AC^GT[GA] BsiHKAI 253 GTGCAC G[AT]GC[AT]^C BsiWI 883 CGTACG C^GTACG BsmBI 1835 GAGACG CGTCTC(1/5) BsmI 1195 GAATGC GAATGC(1/-1) BspHI 1011 TCATGA T^CATGA BsrDI 1308 CATTGC GCAATG(2/0) BstUI 1827 CGCG CG^CG BstYI 14 AGATCT [GA]^GATC[CT] CelII 297 GCTGAGC GC^T[ACGT]AGC DraI 1024 TTTAAA TTT^AAA Eco47III 1360 AGCGCT AGC^GCT EcoNI 78 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 536 TGATCA T^GATCA FspI 1340 TGCGCA TGC^GCA HgaI 176 GCGTC GACGC(5/10) HincII 910 GTTAAC GT[CT]^[GA]AC HindII 910 GTTAAC GT[CT]^[GA]AC HinfI 1928 GAGTC G^A[ACGT]TC HpaI 910 GTTAAC GTT^AAC MflI 14 AGATCT [GA]^GATC[CT] MvnI 1827 CGCG CG^CG NciI 377 CCCGG CC^[GC]GG PflFI 172 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 567 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1928 GAGTC GAGTC(4/5) PvuII 695 CAGCTG CAG^CTG RcaI 1011 TCATGA T^CATGA SapI 550 GAAGAGC GCTCTTC(1/4) SphI 1350 GCATGC GCATG^C StuI 75 AGGCCT AGG^CCT Tth111I 172 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 567 CCAGTTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 14 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcsI 660 AAATTC [GA]^AATT[CT] 1617 AAATTT ApoI 660 AAATTC [GA]^AATT[CT] 1617 AAATTT AvaI 55 CTCGAG C^[CT]CG[GA]G 544 CTCGAG BalI 401 TGGCCA TGG^CCA 485 TGGCCA BanII 126 GAGCCC G[GA]GC[CT]^C 373 GGGCCC BbsI 493 GAAGAC GAAGAC(2/6) 1396 GTCTTC BpmI 181 CTGGAG CTGGAG(16/14) 1526 CTGGAG BpuAI 493 GAAGAC GAAGAC(2/6) 1396 GTCTTC BsmAI 1139 GAGAC GTCTC(1/5) 1835 GAGAC BsoBI 55 CTCGAG C^[CT]CG[GA]G 544 CTCGAG BsrBI 1505 CCGCTC CCGCTC(-3/-3) 1227 GAGCGG EaeI 401 TGGCCA [CT]^GGCC[GA] 485 TGGCCA HapII 67 CCGG C^CGG 378 CCGG HpaII 67 CCGG C^CGG 378 CCGG MaeII 1329 ACGT A^CGT 1494 ACGT MluNI 401 TGGCCA TGG^CCA 485 TGGCCA MscI 401 TGGCCA TGG^CCA 485 TGGCCA MspA1I 695 CAGCTG C[AC]G^C[GT]G 1668 CCGCTG MspI 67 CCGG C^CGG 378 CCGG NspI 785 GCATGT [GA]CATG^[CT] 1350 GCATGC PaeR7I 55 CTCGAG C^TCGAG 544 CTCGAG TaqI 56 TCGA T^CGA 545 TCGA TthHB8I 56 TCGA T^CGA 545 TCGA XbaI 465 TCTAGA T^CTAGA 799 TCTAGA XhoI 55 CTCGAG C^TCGAG 544 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI Asp700I BbvI BfaI BmyI BsaJI BseRI BsgI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MvaI MwoI NdeI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI Tru9I TseI Tsp45I Tsp509I TspRI XmnI