Restriction map for
pFN21AB6201
(Circular) (Six-base) MAPSORT of: pFN21AB6201.seq Check: 1915 from: 1 to: 6694
pFN21AB6201 6694 bp
With 182 enzymes: SgfI *
December 8, 2014 13:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2320 3557 2320
Size: 1237 5457
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4504 278
Size: 53 83 186 936 2968 2468
Fragments arranged by size:
2968 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4037 1937
Size: 2100 4594
Acc65I G'GTAC_C
Cuts at: 4015 4015
Size: 6694
AclI AA'CG_TT
Cuts at: 1563 4821 5194 1563
Size: 3258 373 3063
Fragments arranged by size:
3258 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2105 2292 3586 3720 3800 4757 6141 6409
Size: 473 187 1294 134 80 957 1384 268
Cuts at: 6409 1632
Size: 1917
Fragments arranged by size:
1917 1384 1294 957 473 268 187 134
80
AfeI AGC'GCT
Cuts at: 3367 3367
Size: 6694
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6497
AflIII A'CryG_T
Cuts at: 1203 3334 5593 6570 1203
Size: 2131 2259 977 1327
Fragments arranged by size:
2259 2131 1327 977
AgeI A'CCGG_T
Cuts at: 5525 5525
Size: 6694
AhdI GACnn_n'nnGTC
Cuts at: 5423 5423
Size: 6694
AleI CACnn'nnGTG
Cuts at: 1166 2844 3183 1166
Size: 1678 339 4677
Fragments arranged by size:
4677 1678 339
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6694
AlwNI CAG_nnn'CTG
Cuts at: 2015 2392 2717 4437 6009 2015
Size: 377 325 1720 1572 2700
Fragments arranged by size:
2700 1720 1572 377 325
ApaI G_GGCC'C
Cuts at: 2382 2382
Size: 6694
ApaLI G'TGCA_C
Cuts at: 2258 4751 5907 2258
Size: 2493 1156 3045
Fragments arranged by size:
3045 2493 1156
ApoI r'AATT_y
Cuts at: 1493 2665 3622 4003 4229 4381 6490 1493
Size: 1172 957 381 226 152 2109 1697
Fragments arranged by size:
2109 1697 1172 957 381 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5248 160
Size: 872 4216 1606
Fragments arranged by size:
4216 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
AvaI C'yCGr_G
Cuts at: 1870 1943 2060 2549 4019 1870
Size: 73 117 489 1470 4545
Fragments arranged by size:
4545 1470 489 117 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3505 3538 3505
Size: 33 6661
BamHI G'GATC_C
Cuts at: 4024 4391 4024
Size: 367 6327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4015 5470 618
Size: 325 217 627 2228 1455 1842
Fragments arranged by size:
2228 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2135 2382 4013 1391
Size: 744 247 1631 4072
Fragments arranged by size:
4072 1631 744 247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2505 3394 928
Size: 971 606 889 4228
Fragments arranged by size:
4228 971 889 606
BbvCI CC'TCA_GC
Cuts at: 3855 3855
Size: 6694
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3833 3867 4885 4919 1903
Size: 34 1896 34 1018 34 3678
Fragments arranged by size:
3678 1896 1018 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2426 4586 5796 2426
Size: 2160 1210 3324
Fragments arranged by size:
3324 2160 1210
BclI T'GATC_A
Cuts at: 1548 2541 1548
Size: 993 5701
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5305 136
Size: 107 122 71 631 605 3633 1525
Fragments arranged by size:
3633 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2019 6689 2019
Size: 4670 2024
BlpI GC'TnA_GC
Cuts at: 2303 4111 2303
Size: 1808 4886
Bme1580I G_kGCm'C
Cuts at: 1790 2262 2382 4755 5911 1790
Size: 472 120 2373 1156 2573
Fragments arranged by size:
2573 2373 1156 472 120
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6694
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3108 4425 5383
Size: 416 53 83 399 24 1691 1317 958
Cuts at: 5383 442
Size: 1753
Fragments arranged by size:
1753 1691 1317 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6694
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2449 2176
Size: 273 6421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 3552 5354 1239
Size: 968 1345 1802 2579
Fragments arranged by size:
2579 1802 1345 968
Bpu10I CC'TnA_GC
Cuts at: 3855 3855
Size: 6694
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2390 4819 5684 5982 2390
Size: 2429 865 298 3102
Fragments arranged by size:
3102 2429 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5357 882
Size: 617 3858 2219
Fragments arranged by size:
3858 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3335 4485 493
Size: 711 2131 1150 2702
Fragments arranged by size:
2702 2131 1150 711
BsaBI GATnn'nnATC
Cuts at: 6688 6688
Size: 6694
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4501 4883 5518 275
Size: 53 83 186 936 2968 382 635 1451
Fragments arranged by size:
2968 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5127 5525 5799 5946 6357 5127
Size: 398 274 147 411 5464
Fragments arranged by size:
5464 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2122 2152 2973 3003 3632
Size: 30 165 30 719 30 821 30 629
Cuts at: 3632 3662 1178
Size: 30 4210
Fragments arranged by size:
4210 821 719 629 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2105 2237 2489 3872 3965 1186
Size: 919 132 252 1383 93 3915
Fragments arranged by size:
3915 1383 919 252 132 93
BseYI C'CCAG_C
Cuts at: 5897 6393 5897
Size: 496 6198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2131 2348 3148 3671 1163
Size: 573 395 217 800 523 4186
Fragments arranged by size:
4186 800 573 523 395 217
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4144 4905 5933 1540
Size: 275 186 2143 761 1028 2301
Fragments arranged by size:
2301 2143 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2262 4755 4840 5911 2262
Size: 2493 85 1071 3045
Fragments arranged by size:
3045 2493 1071 85
BsiWI C'GTAC_G
Cuts at: 2888 2888
Size: 6694
BsmI GAATG_Cn'
Cuts at: 3206 4205 4298 3206
Size: 999 93 5602
Fragments arranged by size:
5602 999 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3834 3834
Size: 6694
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2135 2262 2382 4013 4755 4840 5911
Size: 399 345 127 120 1631 742 85 1071
Cuts at: 5911 1391
Size: 2174
Fragments arranged by size:
2174 1631 1071 742 399 345 127 120
85
BspEI T'CCGG_A
Cuts at: 6357 6357
Size: 6694
BspHI T'CATG_A
Cuts at: 3016 4583 6313 3016
Size: 1567 1730 3397
Fragments arranged by size:
3397 1730 1567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2320 3557 3693 4049 844
Size: 1476 1237 136 356 3489
Fragments arranged by size:
3489 1476 1237 356 136
BsrBI CCG'CTC
Cuts at: 1394 1823 3234 3512 4581 1394
Size: 429 1411 278 1069 3507
Fragments arranged by size:
3507 1411 1069 429 278
BsrDI GCAATG_nn'
Cuts at: 65 1248 3312 5189 5363 65
Size: 1183 2064 1877 174 1396
Fragments arranged by size:
2064 1877 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5338 5525 1700
Size: 110 3528 187 2869
Fragments arranged by size:
3528 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6694
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6694
BssSI C'ACGA_G
Cuts at: 4754 5766 4754
Size: 1012 5682
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2257 2392 3474 794
Size: 1463 135 1082 4014
Fragments arranged by size:
4014 1463 1082 135
BstBI TT'CG_AA
Cuts at: 5498 5498
Size: 6694
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6694
BstYI r'GATC_y
Cuts at: 1972 2019 4024 4391 4776 4793 6245 6331 6343
Size: 47 2005 367 385 17 1452 86 12
Cuts at: 6343 6689 1972
Size: 346 1977
Fragments arranged by size:
2005 1977 1452 385 367 346 86 47
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5203
Fragments arranged by size:
5203 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5955
Fragments arranged by size:
5955 683 56
BtsI GCAGTG_nn'
Cuts at: 2405 3472 4210 5004 5024 2405
Size: 1067 738 794 20 4075
Fragments arranged by size:
4075 1067 794 738 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6694
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3295 3330 529
Size: 35 2731 35 3893
Fragments arranged by size:
3893 2731 35 35
DraI TTT'AAA
Cuts at: 3031 3998 3031
Size: 967 5727
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5701 809
Size: 507 4385 1802
Fragments arranged by size:
4385 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2406 2490 4141
Size: 54 1425 50 275 18 576 84 1651
Cuts at: 4141 5030 6659 8
Size: 889 1629 43
Fragments arranged by size:
1651 1629 1425 889 576 275 84 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4141 1537
Size: 275 2329 4090
Fragments arranged by size:
4090 2329 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2550 2806 3449 4624 1353
Size: 1197 256 643 1175 3423
Fragments arranged by size:
3423 1197 1175 643 256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5281 5655 5801 387
Size: 1064 138 3692 374 146 1280
Fragments arranged by size:
3692 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2207 2292 3552 3586 3720 3800
Size: 393 473 102 85 1260 34 134 80
Cuts at: 3800 4757 5354 6141 6409 1239
Size: 957 597 787 268 1524
Fragments arranged by size:
1524 1260 957 787 597 473 393 268
134 102 85 80 34
EcoNI CCTnn'n_nnAGG
Cuts at: 2087 2087
Size: 6694
EcoO109I rG'GnC_Cy
Cuts at: 2166 2378 2379 2937 2166
Size: 212 1 558 5923
Fragments arranged by size:
5923 558 212 1
EcoRI G'AATT_C
Cuts at: 4003 4381 4003
Size: 378 6316
FspI TGC'GCA
Cuts at: 3347 5200 3347
Size: 1853 4841
HaeII r_GCGC'y
Cuts at: 1678 3004 3082 3109 3369 5841 1678
Size: 1326 78 27 260 2472 2531
Fragments arranged by size:
2531 2472 1326 260 78 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2973 3005 5335 5367 5409
Size: 32 135 32 1401 32 2330 32 42
Cuts at: 5409 5441 1373
Size: 32 2626
Fragments arranged by size:
2626 2330 1401 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2917 4038 4284 669
Size: 988 281 979 1121 246 3079
Fragments arranged by size:
3079 1121 988 979 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6694
HpaI GTT'AAC
Cuts at: 2917 4284 2917
Size: 1367 5327
KpnI G_GTAC'C
Cuts at: 4019 4019
Size: 6694
MfeI C'AATT_G
Cuts at: 4293 4293
Size: 6694
MluI A'CGCG_T
Cuts at: 6570 6570
Size: 6694
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2124 3016 3598 5808 5992 1305
Size: 819 892 582 2210 184 2007
Fragments arranged by size:
2210 2007 892 819 582 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2408 2492 10
Size: 54 1425 343 576 84 4212
Fragments arranged by size:
4212 1425 576 343 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2781 2844 3015 3183 3447 4652 5011
Size: 648 1615 63 171 168 264 1205 359
Cuts at: 5011 5170 518
Size: 159 2042
Fragments arranged by size:
2042 1615 1205 648 359 264 171 168
159 63
MspA1I CmG'CkG
Cuts at: 1606 2702 3675 4110 4787 5935 6180 1606
Size: 1096 973 435 677 1148 245 2120
Fragments arranged by size:
2120 1148 1096 973 677 435 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5203
Fragments arranged by size:
5203 939 552
NdeI CA'TA_TG
Cuts at: 387 2829 3342 3504 387
Size: 2442 513 162 3577
Fragments arranged by size:
3577 2442 513 162
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6694
NotI GC'GGCC_GC
Cuts at: 4141 4141
Size: 6694
NspI r_CATG'y
Cuts at: 1132 1229 2794 3359 4052 5597 1132
Size: 97 1565 565 693 1545 2229
Fragments arranged by size:
2229 1565 1545 693 565 97
PasI CC'CwG_GG
Cuts at: 1348 2354 1348
Size: 1006 5688
PciI A'CATG_T
Cuts at: 5593 5593
Size: 6694
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2578 1108
Size: 1470 5224
PfoI T'CCnGG_A
Cuts at: 2172 2184 2172
Size: 12 6682
PmeI GTTT'AAAC
Cuts at: 3998 3998
Size: 6694
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3443 3475 4763 4795 6276 6308 3443
Size: 32 1288 32 1481 32 3829
Fragments arranged by size:
3829 1481 1288 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6694
PsiI TTA'TAA
Cuts at: 4264 4264
Size: 6694
PspOMI G'GGCC_C
Cuts at: 2378 2378
Size: 6694
PspXI vC'TCGA_Gb
Cuts at: 2060 2549 2060
Size: 489 6205
PstI C_TGCA'G
Cuts at: 830 2253 2397 3261 3373 3479 3917 4046 830
Size: 1423 144 864 112 106 438 129 3478
Fragments arranged by size:
3478 1423 864 438 144 129 112 106
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6694
PvuII CAG'CTG
Cuts at: 2702 2702
Size: 6694
SalI G'TCGA_C
Cuts at: 1936 4036 1936
Size: 2100 4594
SapI GCTCTTCn'nnn_
Cuts at: 2550 2550
Size: 6694
SbfI CC_TGCA'GG
Cuts at: 4046 4046
Size: 6694
ScaI AGT'ACT
Cuts at: 4942 6654 4942
Size: 1712 4982
SfcI C'TryA_G
Cuts at: 826 1045 2249 2393 2697 3257 3369 3475 3913
Size: 219 1204 144 304 560 112 106 438
Cuts at: 3913 4042 5177 5858 6049 826
Size: 129 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 681 560 438 304 219
191 144 129 112 106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6694
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6694
SmaI CCC'GGG
Cuts at: 1872 4021 1872
Size: 2149 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2060 2369 2549 4798 5699 5961
Size: 197 926 117 309 180 2249 901 262
Cuts at: 5961 820
Size: 1553
Fragments arranged by size:
2249 1553 926 901 309 262 197 180
117
SnaBI TAC'GTA
Cuts at: 493 4485 493
Size: 3992 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6694
SphI G_CATG'C
Cuts at: 1132 3359 4052 1132
Size: 2227 693 3774
Fragments arranged by size:
3774 2227 693
SspI AAT'ATT
Cuts at: 5 52 4618 5
Size: 47 4566 2081
Fragments arranged by size:
4566 2081 47
StuI AGG'CCT
Cuts at: 1517 2082 1517
Size: 565 6129
StyI C'CwwG_G
Cuts at: 513 1065 2004 4133 513
Size: 552 939 2129 3074
Fragments arranged by size:
3074 2129 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2936 3242 4721 4738 4891 5076 5521
Size: 1255 1356 306 1479 17 153 185 445
Cuts at: 5521 325
Size: 1498
Fragments arranged by size:
1498 1479 1356 1255 445 306 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 2430 4940
Size: 274 80 33 51 1443 258 195 2510
Cuts at: 4940 6652 96
Size: 1712 138
Fragments arranged by size:
2510 1712 1443 274 258 195 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2122 2154 6654 6686 2122
Size: 32 4500 32 2130
Fragments arranged by size:
4500 2130 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2180 1316
Size: 864 5830
XbaI T'CTAG_A
Cuts at: 2470 2804 4030 2470
Size: 334 1226 5134
Fragments arranged by size:
5134 1226 334
XhoI C'TCGA_G
Cuts at: 1943 2060 2549 1943
Size: 117 489 6088
Fragments arranged by size:
6088 489 117
XmaI C'CCGG_G
Cuts at: 1870 4019 1870
Size: 2149 4545
XmnI GAAnn'nnTTC
Cuts at: 2649 2882 3458 3542 4823 2649
Size: 233 576 84 1281 4520
Fragments arranged by size:
4520 1281 576 233 84
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4502 276
Size: 53 83 186 936 2968 2468
Fragments arranged by size:
2968 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BbeI BfrBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PsrI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI XcmI