Restriction map for
pFN21AB6201
(Linear) (Six-base) MAPSORT of: KIBB6201.seq Check: 1775 from: 1 to: 1989
KIBB6201.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 315 1552 1989
Size: 315 1237 437
Fragments arranged by size:
1237 437 315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 287 1581 1715 1795 1989
Size: 100 187 1294 134 80 194
Fragments arranged by size:
1294 194 187 134 100 80
AfeI AGC'GCT
Cuts at: 0 1362 1989
Size: 1362 627
AflIII A'CryG_T
Cuts at: 0 1329 1989
Size: 1329 660
AleI CACnn'nnGTG
Cuts at: 0 839 1178 1989
Size: 839 339 811
Fragments arranged by size:
839 811 339
AlwNI CAG_nnn'CTG
Cuts at: 0 10 387 712 1989
Size: 10 377 325 1277
Fragments arranged by size:
1277 377 325 10
ApaI G_GGCC'C
Cuts at: 0 377 1989
Size: 377 1612
ApaLI G'TGCA_C
Cuts at: 0 253 1989
Size: 253 1736
ApoI r'AATT_y
Cuts at: 0 660 1617 1989
Size: 660 957 372
Fragments arranged by size:
957 660 372
AvaI C'yCGr_G
Cuts at: 0 55 544 1989
Size: 55 489 1445
Fragments arranged by size:
1445 489 55
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1500 1533 1989
Size: 1500 33 456
Fragments arranged by size:
1500 456 33
BanII G_rGCy'C
Cuts at: 0 130 377 1989
Size: 130 247 1612
Fragments arranged by size:
1612 247 130
BbsI GAAGACnn'nnnn_
Cuts at: 0 500 1389 1989
Size: 500 889 600
Fragments arranged by size:
889 600 500
BbvCI CC'TCA_GC
Cuts at: 0 1850 1989
Size: 1850 139
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1828 1862 1989
Size: 1828 34 127
Fragments arranged by size:
1828 127 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 421 1989
Size: 421 1568
BclI T'GATC_A
Cuts at: 0 536 1989
Size: 536 1453
BglII A'GATC_T
Cuts at: 0 14 1989
Size: 14 1975
BlpI GC'TnA_GC
Cuts at: 0 298 1989
Size: 298 1691
Bme1580I G_kGCm'C
Cuts at: 0 257 377 1989
Size: 257 120 1612
Fragments arranged by size:
1612 257 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 1103 1989
Size: 1103 886
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 171 444 1989
Size: 171 273 1545
Fragments arranged by size:
1545 273 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 1547 1989
Size: 202 1345 442
Fragments arranged by size:
1345 442 202
Bpu10I CC'TnA_GC
Cuts at: 0 1850 1989
Size: 1850 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 1989
Size: 385 1604
BsaAI yAC'GTr
Cuts at: 0 1330 1989
Size: 1330 659
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 117 147 968 998 1627 1657 1989
Size: 117 30 821 30 629 30 332
Fragments arranged by size:
821 629 332 117 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 232 484 1867 1960 1989
Size: 100 132 252 1383 93 29
Fragments arranged by size:
1383 252 132 100 93 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 343 1143 1666 1989
Size: 126 217 800 523 323
Fragments arranged by size:
800 523 323 217 126
BsiHKAI G_wGCw'C
Cuts at: 0 257 1989
Size: 257 1732
BsiWI C'GTAC_G
Cuts at: 0 883 1989
Size: 883 1106
BsmI GAATG_Cn'
Cuts at: 0 1201 1989
Size: 1201 788
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1829 1989
Size: 1829 160
Bsp1286I G_dGCh'C
Cuts at: 0 130 257 377 1989
Size: 130 127 120 1612
Fragments arranged by size:
1612 130 127 120
BspHI T'CATG_A
Cuts at: 0 1011 1989
Size: 1011 978
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 315 1552 1688 1989
Size: 315 1237 136 301
Fragments arranged by size:
1237 315 301 136
BsrBI CCG'CTC
Cuts at: 0 1229 1507 1989
Size: 1229 278 482
Fragments arranged by size:
1229 482 278
BsrDI GCAATG_nn'
Cuts at: 0 1307 1989
Size: 1307 682
BstAPI GCAn_nnn'nTGC
Cuts at: 0 252 387 1469 1989
Size: 252 135 1082 520
Fragments arranged by size:
1082 520 252 135
BstYI r'GATC_y
Cuts at: 0 14 1989
Size: 14 1975
BtsI GCAGTG_nn'
Cuts at: 0 400 1467 1989
Size: 400 1067 522
Fragments arranged by size:
1067 522 400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1290 1325 1989
Size: 1290 35 664
Fragments arranged by size:
1290 664 35
DraI TTT'AAA
Cuts at: 0 1026 1989
Size: 1026 963
EaeI y'GGCC_r
Cuts at: 0 401 485 1989
Size: 401 84 1504
Fragments arranged by size:
1504 401 84
EarI CTCTTCn'nnn_
Cuts at: 0 545 801 1444 1989
Size: 545 256 643 545
Fragments arranged by size:
643 545 545 256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 202 287 1547 1581 1715 1795 1989
Size: 100 102 85 1260 34 134 80 194
Fragments arranged by size:
1260 194 134 102 100 85 80 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 82 1989
Size: 82 1907
EcoO109I rG'GnC_Cy
Cuts at: 0 161 373 374 932 1989
Size: 161 212 1 558 1057
Fragments arranged by size:
1057 558 212 161 1
FspI TGC'GCA
Cuts at: 0 1342 1989
Size: 1342 647
HaeII r_GCGC'y
Cuts at: 0 999 1077 1104 1364 1989
Size: 999 78 27 260 625
Fragments arranged by size:
999 625 260 78 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 968 1000 1989
Size: 968 32 989
Fragments arranged by size:
989 968 32
HincII GTy'rAC
Cuts at: 0 912 1989
Size: 912 1077
HpaI GTT'AAC
Cuts at: 0 912 1989
Size: 912 1077
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1011 1593 1989
Size: 119 892 582 396
Fragments arranged by size:
892 582 396 119
MscI TGG'CCA
Cuts at: 0 403 487 1989
Size: 403 84 1502
Fragments arranged by size:
1502 403 84
MslI CAynn'nnrTG
Cuts at: 0 776 839 1010 1178 1442 1989
Size: 776 63 171 168 264 547
Fragments arranged by size:
776 547 264 171 168 63
MspA1I CmG'CkG
Cuts at: 0 697 1670 1989
Size: 697 973 319
Fragments arranged by size:
973 697 319
NdeI CA'TA_TG
Cuts at: 0 824 1337 1499 1989
Size: 824 513 162 490
Fragments arranged by size:
824 513 490 162
NspI r_CATG'y
Cuts at: 0 789 1354 1989
Size: 789 565 635
Fragments arranged by size:
789 635 565
PasI CC'CwG_GG
Cuts at: 0 349 1989
Size: 349 1640
PflMI CCAn_nnn'nTGG
Cuts at: 0 573 1989
Size: 573 1416
PfoI T'CCnGG_A
Cuts at: 0 167 179 1989
Size: 167 12 1810
Fragments arranged by size:
1810 167 12
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1438 1470 1989
Size: 1438 32 519
Fragments arranged by size:
1438 519 32
PspOMI G'GGCC_C
Cuts at: 0 373 1989
Size: 373 1616
PspXI vC'TCGA_Gb
Cuts at: 0 55 544 1989
Size: 55 489 1445
Fragments arranged by size:
1445 489 55
PstI C_TGCA'G
Cuts at: 0 248 392 1256 1368 1474 1912 1989
Size: 248 144 864 112 106 438 77
Fragments arranged by size:
864 438 248 144 112 106 77
PvuII CAG'CTG
Cuts at: 0 697 1989
Size: 697 1292
SapI GCTCTTCn'nnn_
Cuts at: 0 545 1989
Size: 545 1444
SfcI C'TryA_G
Cuts at: 0 244 388 692 1252 1364 1470 1908 1989
Size: 244 144 304 560 112 106 438 81
Fragments arranged by size:
560 438 304 244 144 112 106 81
SmlI C'TyrA_G
Cuts at: 0 55 364 544 1989
Size: 55 309 180 1445
Fragments arranged by size:
1445 309 180 55
SphI G_CATG'C
Cuts at: 0 1354 1989
Size: 1354 635
StuI AGG'CCT
Cuts at: 0 77 1989
Size: 77 1912
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 931 1237 1989
Size: 931 306 752
Fragments arranged by size:
931 752 306
TatI w'GTAC_w
Cuts at: 0 230 425 1989
Size: 230 195 1564
Fragments arranged by size:
1564 230 195
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 117 149 1989
Size: 117 32 1840
Fragments arranged by size:
1840 117 32
Tth111I GACn'n_nGTC
Cuts at: 0 175 1989
Size: 175 1814
XbaI T'CTAG_A
Cuts at: 0 465 799 1989
Size: 465 334 1190
Fragments arranged by size:
1190 465 334
XhoI C'TCGA_G
Cuts at: 0 55 544 1989
Size: 55 489 1445
Fragments arranged by size:
1445 489 55
XmnI GAAnn'nnTTC
Cuts at: 0 644 877 1453 1537 1989
Size: 644 233 576 84 452
Fragments arranged by size:
644 576 452 233 84
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BaeI BanII BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaXI BseRI BsgI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstYI
BtsI CspCI DraI EaeI EarI Eco57MI EcoNI EcoO109I
FspI HaeII Hin4I HincII HpaI MmeI MscI MslI
MspA1I NdeI NspI PasI PflMI PfoI PpiI PspOMI
PspXI PstI PvuII SapI SfcI SmlI SphI StuI
TaqII TatI TstI Tth111I XbaI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BamHI BanI BbeI
BfrBI BglI BmgBI BmtI BsaI BsaBI BsaHI BsaWI
BseYI BsiEI BspEI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspAI HindIII KasI KpnI MfeI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpuMI PshAI PsiI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StyI SwaI XcmI XmaI ZraI