################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssSI Bst1107I BstAPI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DpnII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI HphI KpnI Ksp632I MaeII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MunI NaeI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AlwNI 259 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG AseI 324 ATTAAT AT^TAAT AsnI 324 ATTAAT AT^TAAT AvaI 47 CCCGGG C^[CT]CG[GA]G BmyI 514 GTGCCC G[AGT]GC[ACT]^C BpmI 560 CTCCAG CTGGAG(16/14) BseRI 235 GAGGAG GAGGAG(10/8) BsgI 228 CTGCAC GTGCAG(16/14) BsmAI 508 GTCTC GTCTC(1/5) BsoBI 47 CCCGGG C^[CT]CG[GA]G Bsp1286I 514 GTGCCC G[AGT]GC[ACT]^C BsrDI 305 CATTGC GCAATG(2/0) BsrI 484 CCAGT ACTGG(1/-1) BssHII 160 GCGCGC G^CGCGC BstBI 536 TTCGAA TT^CGAA DraII 185 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 185 AGGACCC [GA]G^G[ACGT]CC[CT] EcoT14I 332 CCTTGG C^C[AT][AT]GG KspI 174 CCGCGG CCGC^GG MaeIII 255 GTCAC ^GT[ACGT]AC MslI 348 CATGGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 371 ACATGC [GA]CATG^[CT] NspV 536 TTCGAA TT^CGAA PleI 406 GAGTC GAGTC(4/5) PpuMI 185 AGGACCC [GA]G^G[AT]CC[CT] PshBI 324 ATTAAT AT^TAAT SacII 174 CCGCGG CCGC^GG SfuI 536 TTCGAA TT^CGAA SmaI 47 CCCGGG CCC^GGG StyI 332 CCTTGG C^C[AT][AT]GG TaqI 537 TCGA T^CGA Tsp45I 255 GTCAC ^GT[GC]AC Tsp509I 327 AATT ^AATT TthHB8I 537 TCGA T^CGA XcmI 65 CCACCTGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 47 CCCGGG C^CCGGG XmaI 47 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 415 GTAC GT^AC 443 GTAC BanI 43 GGCGCC G^G[CT][GA]CC 79 GGCGCC BbeI 43 GGCGCC GGCGC^C 79 GGCGCC BfaI 136 CTAG C^TAG 505 CTAG DraI 400 TTTAAA TTT^AAA 553 TTTAAA DsaI 174 CCGCGG C^C[GA][CT]GG 200 CCACGG EaeI 2 TGGCCG [CT]^GGCC[GA] 177 CGGCCA HaeII 43 GGCGCC [GA]GCGC^[CT] 79 GGCGCC HgaI 139 GACGC GACGC(5/10) 221 GCGTC KasI 43 GGCGCC G^GCGCC 79 GGCGCC MaeI 136 CTAG C^TAG 505 CTAG NarI 43 GGCGCC GG^CGCC 79 GGCGCC NlaIII 348 CATG CATG^ 372 CATG PvuII 225 CAGCTG CAG^CTG 563 CAGCTG RsaI 415 GTAC GT^AC 443 GTAC SfoI 43 GGCGCC GGC^GCC 79 GGCGCC TfiI 317 GAATC G^A[AT]TC 491 GAATC TspRI 291 CACTG CA[GC]TG[ACGT][ACGT]^ 485 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AccII AciI AcyI AluI AvaII BbvI BcnI BsaHI BsaJI BsiYI BslI BsmFI BssKI BstNI BstUI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII ItaI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI Tru9I TseI