################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AviII AvrII BbeI BbrPI BbvCI BclI BfaI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI KpnI KspI MaeI MamI MfeI MroI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SacII ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 78 CGCG CG^CG AflII 961 CTTAAG C^TTAAG AflIII 77 ACGCGT A^C[GA][CT]GT Alw44I 1572 GTGCAC G^TGCAC AlwNI 1451 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 1572 GTGCAC G^TGCAC BalI 176 TGGCCA TGG^CCA BamHI 504 GGATCC G^GATCC BanII 1491 GGGCTC G[GA]GC[CT]^C BciVI 1723 GTATCC GTATCC(6/5) BfrI 961 CTTAAG C^TTAAG BglI 643 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 1268 CTGCAC GTGCAG(16/14) BspHI 860 TCATGA T^CATGA BspMI 1068 ACCTGC ACCTGC(4/8) BstAPI 262 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 4 GGTAACC G^GT[ACGT]ACC BstPI 4 GGTAACC G^GT[ACGT]ACC BstUI 78 CGCG CG^CG BstXI 999 CCAAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 504 GGATCC [GA]^GATC[CT] DsaI 1586 CCACGG C^C[GA][CT]GG EaeI 176 TGGCCA [CT]^GGCC[GA] EarI 1536 GAAGAG CTCTTC(1/4) EcoO65I 4 GGTAACC G^GT[ACGT]ACC EcoRI 1014 GAATTC G^AATTC HgaI 79 GCGTC GACGC(5/10) HindIII 358 AAGCTT A^AGCTT Ksp632I 1536 GAAGAG CTCTTC(1/4) MaeII 315 ACGT A^CGT MflI 504 GGATCC [GA]^GATC[CT] MluI 77 ACGCGT A^CGCGT MluNI 176 TGGCCA TGG^CCA MscI 176 TGGCCA TGG^CCA MvnI 78 CGCG CG^CG NlaIII 861 CATG CATG^ PshAI 1343 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 860 TCATGA T^CATGA SapI 1536 GAAGAGC GCTCTTC(1/4) SbfI 712 CCTGCAGG CCTGCA^GG Sse8387I 712 CCTGCAGG CCTGCA^GG StuI 1674 AGGCCT AGG^CCT XcmI 400 CCAAGTGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 504 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 111 GTCGAC GT^[AC][GT]AC 142 GTCGAC AcsI 586 AAATTC [GA]^AATT[CT] 1014 GAATTC AfaI 307 GTAC GT^AC 317 GTAC ApoI 586 AAATTC [GA]^AATT[CT] 1014 GAATTC AspHI 1469 GAGCAC G[AT]GC[AT]^C 1572 GTGCAC AspI 481 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1153 GACTCAGTC AvaI 190 CCCGAG C^[CT]CG[GA]G 256 CCCGAG BbsI 34 GAAGAC GAAGAC(2/6) 919 GAAGAC BcgI 1257 CGAAGGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1598 CGAACTCACTGC BcnI 436 CCGGG CC^[GC]GG 471 CCCGG BpuAI 34 GAAGAC GAAGAC(2/6) 919 GAAGAC BsaWI 319 ACCGGA [AT]^CCGG[AT] 420 TCCGGT BsiHKAI 1469 GAGCAC G[AT]GC[AT]^C 1572 GTGCAC BsoBI 190 CCCGAG C^[CT]CG[GA]G 256 CCCGAG DraI 871 TTTAAA TTT^AAA 1669 TTTAAA EcoNI 304 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 523 CCTGAGACAGG NciI 436 CCGGG CC^[GC]GG 471 CCCGG PflFI 481 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1153 GACTCAGTC PstI 713 CTGCAG CTGCA^G 1606 CTGCAG RsaI 307 GTAC GT^AC 317 GTAC SalI 111 GTCGAC G^TCGAC 142 GTCGAC SmlI 961 CTTAAG C^T[CT][GA]AG 1703 CTCAAG Tth111I 481 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1153 GACTCAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AhdI AluI AlwI AspEI AvaII BanI BbvI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII Eam1105I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIV PleI PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I