Restriction map for
pFN21AE5634
(Circular) (Six-base) MAPSORT of: pFN21AE5634.seq Check: 1840 from: 1 to: 6457
pFN21AE5634 6457 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4267 278
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2117 2148 3800 1937
Size: 180 31 1652 4594
Fragments arranged by size:
4594 1652 180 31
Acc65I G'GTAC_C
Cuts at: 3778 3778
Size: 6457
AclI AA'CG_TT
Cuts at: 1563 4584 4957 1563
Size: 3021 373 3063
Fragments arranged by size:
3063 3021 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2940 4520 5904 6172 1632
Size: 1308 1580 1384 268 1917
Fragments arranged by size:
1917 1580 1384 1308 268
AflII C'TTAA_G
Cuts at: 820 1017 2966 820
Size: 197 1949 4311
Fragments arranged by size:
4311 1949 197
AflIII A'CryG_T
Cuts at: 1203 2082 5356 6333 1203
Size: 879 3274 977 1327
Fragments arranged by size:
3274 1327 977 879
AgeI A'CCGG_T
Cuts at: 5288 5288
Size: 6457
AhdI GACnn_n'nnGTC
Cuts at: 2083 2968 3514 5186 2083
Size: 885 546 1672 3354
Fragments arranged by size:
3354 1672 885 546
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6457
AlwNI CAG_nnn'CTG
Cuts at: 3461 4200 5772 3461
Size: 739 1572 4146
Fragments arranged by size:
4146 1572 739
ApaLI G'TGCA_C
Cuts at: 3577 4514 5670 3577
Size: 937 1156 4364
Fragments arranged by size:
4364 1156 937
ApoI r'AATT_y
Cuts at: 1493 2591 3019 3766 3992 4144 6253 1493
Size: 1098 428 747 226 152 2109 1697
Fragments arranged by size:
2109 1697 1098 747 428 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5011 160
Size: 872 3979 1606
Fragments arranged by size:
3979 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
AvaI C'yCGr_G
Cuts at: 1870 1943 2195 2261 3782 1870
Size: 73 252 66 1521 4545
Fragments arranged by size:
4545 1521 252 73 66
BamHI G'GATC_C
Cuts at: 2509 3787 4154 2509
Size: 1278 367 4812
Fragments arranged by size:
4812 1278 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2266 2623 3086 3778 5233
Size: 325 217 627 479 357 463 692 1455
Cuts at: 5233 618
Size: 1842
Fragments arranged by size:
1842 1455 692 627 479 463 357 325
217
BanII G_rGCy'C
Cuts at: 1391 3500 3776 1391
Size: 2109 276 4072
Fragments arranged by size:
4072 2109 276
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2046 2931 928
Size: 971 147 885 4454
Fragments arranged by size:
4454 971 885 147
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3251 3285 3592 3626 4648 4682 1903
Size: 34 1314 34 307 34 1022 34 3678
Fragments arranged by size:
3678 1314 1022 307 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3739 4349 5559 3739
Size: 610 1210 4637
Fragments arranged by size:
4637 1210 610
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6457
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2654 5068 136
Size: 107 122 71 631 605 982 2414 1525
Fragments arranged by size:
2414 1525 982 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6452 6452
Size: 6457
BlpI GC'TnA_GC
Cuts at: 3874 3874
Size: 6457
Bme1580I G_kGCm'C
Cuts at: 1790 2472 3048 3581 4518 5674 1790
Size: 682 576 533 937 1156 2573
Fragments arranged by size:
2573 1156 937 682 576 533
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3568 3719 4188
Size: 416 53 83 399 24 2151 151 469
Cuts at: 4188 5146 442
Size: 958 1753
Fragments arranged by size:
2151 1753 958 469 416 399 151 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3517 3517
Size: 6457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2373 2560 2828 3394 5117 1239
Size: 1134 187 268 566 1723 2579
Fragments arranged by size:
2579 1723 1134 566 268 187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3693 4582 5447 5745 3693
Size: 889 865 298 4405
Fragments arranged by size:
4405 889 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2322 2993 3631 5120 882
Size: 617 823 671 638 1489 2219
Fragments arranged by size:
2219 1489 823 671 638 617
BsaAI yAC'GTr
Cuts at: 493 1204 4248 493
Size: 711 3044 2702
Fragments arranged by size:
3044 2702 711
BsaBI GATnn'nnATC
Cuts at: 6451 6451
Size: 6457
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4264 4646 5281 275
Size: 53 83 186 936 2731 382 635 1451
Fragments arranged by size:
2731 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2324 2425 4890 5288 5562 5709 6120 2324
Size: 101 2465 398 274 147 411 2661
Fragments arranged by size:
2661 2465 411 398 274 147 101
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6232
Fragments arranged by size:
6232 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2562 3194 3490 1186
Size: 1376 632 296 4153
Fragments arranged by size:
4153 1376 632 296
BseYI C'CCAG_C
Cuts at: 5660 6156 5660
Size: 496 5961
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3258 1163
Size: 573 1522 4362
Fragments arranged by size:
4362 1522 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3907 4668 5696 1540
Size: 275 186 1906 761 1028 2301
Fragments arranged by size:
2301 1906 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3478 3581 4518 4603 5674 3478
Size: 103 937 85 1071 4261
Fragments arranged by size:
4261 1071 937 103 85
BsmI GAATG_Cn'
Cuts at: 3968 4061 3968
Size: 93 6364
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2472 3048 3478 3500 3581 3776 4518
Size: 399 682 576 430 22 81 195 742
Cuts at: 4518 4603 5674 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 682 576 430 399 195
85 81 22
BspEI T'CCGG_A
Cuts at: 6120 6120
Size: 6457
BspHI T'CATG_A
Cuts at: 2865 4346 6076 2865
Size: 1481 1730 3246
Fragments arranged by size:
3246 1730 1481
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3082 3812 844
Size: 2238 730 3489
Fragments arranged by size:
3489 2238 730
BsrBI CCG'CTC
Cuts at: 1394 1823 4344 1394
Size: 429 2521 3507
Fragments arranged by size:
3507 2521 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4952 5126 65
Size: 1183 3704 174 1396
Fragments arranged by size:
3704 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5101 5288 1700
Size: 110 3291 187 2869
Fragments arranged by size:
3291 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6457
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6457
BssSI C'ACGA_G
Cuts at: 4517 5529 4517
Size: 1012 5445
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2273 794
Size: 1479 4978
BstBI TT'CG_AA
Cuts at: 5261 5261
Size: 6457
BstEII G'GTnAC_C
Cuts at: 1184 2009 1184
Size: 825 5632
BstXI CCAn_nnnn'nTGG
Cuts at: 3011 3011
Size: 6457
BstYI r'GATC_y
Cuts at: 1972 2509 3787 4154 4539 4556 6008 6094 6106
Size: 537 1278 367 385 17 1452 86 12
Cuts at: 6106 6452 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1278 537 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3591 513
Size: 552 939 1587 3379
Fragments arranged by size:
3379 1587 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5718
Fragments arranged by size:
5718 683 56
BtsI GCAGTG_nn'
Cuts at: 3608 3973 4767 4787 3608
Size: 365 794 20 5278
Fragments arranged by size:
5278 794 365 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6457
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6422
DraI TTT'AAA
Cuts at: 2878 3676 3761 2878
Size: 798 85 5574
Fragments arranged by size:
5574 798 85
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5464 809
Size: 507 4148 1802
Fragments arranged by size:
4148 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2181 3904 4793
Size: 54 1425 50 275 18 351 1723 889
Cuts at: 4793 6422 8
Size: 1629 43
Fragments arranged by size:
1723 1629 1425 889 351 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3904 1537
Size: 275 2092 4090
Fragments arranged by size:
4090 2092 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3536 4387 1353
Size: 2183 851 3423
Fragments arranged by size:
3423 2183 851
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5044 5418 5564 387
Size: 1064 138 3455 374 146 1280
Fragments arranged by size:
3455 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2373 2560 2828 2940 3394 4520 5117
Size: 393 741 187 268 112 454 1126 597
Cuts at: 5117 5904 6172 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1126 787 741 597 454 393 268
268 187 112
EcoNI CCTnn'n_nnAGG
Cuts at: 2313 2532 2313
Size: 219 6238
EcoO109I rG'GnC_Cy
Cuts at: 2491 2794 2917 3104 2491
Size: 303 123 187 5844
Fragments arranged by size:
5844 303 187 123
EcoRI G'AATT_C
Cuts at: 3019 3766 4144 3019
Size: 747 378 5332
Fragments arranged by size:
5332 747 378
FspI TGC'GCA
Cuts at: 4963 4963
Size: 6457
HaeII r_GCGC'y
Cuts at: 1678 5604 1678
Size: 3926 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 2287 2319 2360
Size: 32 135 32 497 32 186 32 41
Cuts at: 2360 2392 2954 2986 3500 3532 3590 3622 3713
Size: 32 562 32 514 32 58 32 91
Cuts at: 3713 3745 5098 5130 5172 5204 1373
Size: 32 1353 32 42 32 2626
Fragments arranged by size:
2626 1353 562 514 497 186 135 91
58 42 41 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2118 2149 2737 3801 4047 669
Size: 988 281 180 31 588 1064 246 3079
Fragments arranged by size:
3079 1064 988 588 281 246 180 31
HindIII A'AGCT_T
Cuts at: 748 2363 748
Size: 1615 4842
HpaI GTT'AAC
Cuts at: 4047 4047
Size: 6457
KpnI G_GTAC'C
Cuts at: 3782 3782
Size: 6457
MfeI C'AATT_G
Cuts at: 4056 4056
Size: 6457
MluI A'CGCG_T
Cuts at: 2082 6333 2082
Size: 4251 2206
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3681 5571 5755 1305
Size: 2376 1890 184 2007
Fragments arranged by size:
2376 2007 1890 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2183 10
Size: 54 1425 343 351 4284
Fragments arranged by size:
4284 1425 351 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4415 4774 4933 518
Size: 648 3249 359 159 2042
Fragments arranged by size:
3249 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2273 2435 2960 3414 3873 4550 5698 5943
Size: 667 162 525 454 459 677 1148 245
Cuts at: 5943 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 667 525 459 454 245
162
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4966
Fragments arranged by size:
4966 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6457
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6457
NotI GC'GGCC_GC
Cuts at: 3904 3904
Size: 6457
NspI r_CATG'y
Cuts at: 1132 1229 3815 5360 1132
Size: 97 2586 1545 2229
Fragments arranged by size:
2586 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2465 2789 1348
Size: 1117 324 5016
Fragments arranged by size:
5016 1117 324
PciI A'CATG_T
Cuts at: 5356 5356
Size: 6457
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6457
PmeI GTTT'AAAC
Cuts at: 3761 3761
Size: 6457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4526 4558 6039 6071 4526
Size: 32 1481 32 4912
Fragments arranged by size:
4912 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2491 2794 2917 3104 2491
Size: 303 123 187 5844
Fragments arranged by size:
5844 303 187 123
PshAI GACnn'nnGTC
Cuts at: 1613 3352 1613
Size: 1739 4718
PsiI TTA'TAA
Cuts at: 3215 4027 3215
Size: 812 5645
PstI C_TGCA'G
Cuts at: 830 2722 3615 3809 830
Size: 1892 893 194 3478
Fragments arranged by size:
3478 1892 893 194
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6457
PvuII CAG'CTG
Cuts at: 2273 2435 2960 3414 2273
Size: 162 525 454 5316
Fragments arranged by size:
5316 525 454 162
SalI G'TCGA_C
Cuts at: 1936 2116 2147 3799 1936
Size: 180 31 1652 4594
Fragments arranged by size:
4594 1652 180 31
SanDI GG'GwC_CC
Cuts at: 2794 2794
Size: 6457
SapI GCTCTTCn'nnn_
Cuts at: 3536 3536
Size: 6457
SbfI CC_TGCA'GG
Cuts at: 2722 3809 2722
Size: 1087 5370
ScaI AGT'ACT
Cuts at: 4705 6417 4705
Size: 1712 4745
SfcI C'TryA_G
Cuts at: 826 1045 2718 2841 3611 3805 4940 5621 5812
Size: 219 1673 123 770 194 1135 681 191
Cuts at: 5812 826
Size: 1471
Fragments arranged by size:
1673 1471 1135 770 681 219 194 191
123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6457
SmaI CCC'GGG
Cuts at: 1872 3784 1872
Size: 1912 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2966 3708 4561 5462 5724 820
Size: 197 926 1023 742 853 901 262 1553
Fragments arranged by size:
1553 1023 926 901 853 742 262 197
SnaBI TAC'GTA
Cuts at: 493 4248 493
Size: 3755 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6457
SphI G_CATG'C
Cuts at: 1132 3815 1132
Size: 2683 3774
SspI AAT'ATT
Cuts at: 5 52 4381 5
Size: 47 4329 2081
Fragments arranged by size:
4329 2081 47
StuI AGG'CCT
Cuts at: 1517 3681 1517
Size: 2164 4293
StyI C'CwwG_G
Cuts at: 513 1065 2004 2350 2568 3004 3090 3222 3661
Size: 552 939 346 218 436 86 132 439
Cuts at: 3661 3753 3896 513
Size: 92 143 3074
Fragments arranged by size:
3074 939 552 439 436 346 218 143
132 92 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2962 3395 4484 4501 4654 4839 5284
Size: 1255 1382 433 1089 17 153 185 445
Cuts at: 5284 325
Size: 1498
Fragments arranged by size:
1498 1382 1255 1089 445 433 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4703 6415 96
Size: 274 80 33 51 1443 2726 1712 138
Fragments arranged by size:
2726 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3038 3070 6417 6449 3038
Size: 32 3347 32 3046
Fragments arranged by size:
3347 3046 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2489 3161 1316
Size: 1173 672 4612
Fragments arranged by size:
4612 1173 672
XbaI T'CTAG_A
Cuts at: 3793 3793
Size: 6457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2412 2412
Size: 6457
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6457
XmaI C'CCGG_G
Cuts at: 1870 3782 1870
Size: 1912 4545
XmnI GAAnn'nnTTC
Cuts at: 4586 4586
Size: 6457
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4265 276
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI
SwaI