Restriction map for
pFN21AE4125
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BbrPI BbvCI
BciVI BclI BfaI BfrI BglI
BglII BlnI BsaI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrFI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DsaI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HindIII HpaI KpnI KspI
MaeI MfeI MflI MluI MseI
MunI NaeI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI StyI SwaI
TfiI Tru9I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 325 GACGTC GACGT^C
AccIII 1436 TCCGGA T^CCGGA
AflIII 308 ACGTGT A^C[GA][CT]GT
AlwI 191 GGATC GGATC(4/5)
AocI 753 CCTGAGG CC^T[ACGT]AGG
ApaI 591 GGGCCC GGGCC^C
AscI 1068 GGCGCGCC GG^CGCGCC
AspI 1285 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1043 TGCGCA TGC^GCA
BbsI 802 GTCTTC GAAGAC(2/6)
BpuAI 802 GTCTTC GAAGAC(2/6)
BsaAI 1054 TACGTG [CT]AC^GT[GA]
BsaBI 192 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1436 TCCGGA [AT]^CCGG[AT]
BseAI 1436 TCCGGA T^CCGGA
BspEI 1436 TCCGGA T^CCGGA
BstAPI 129 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 753 CCTGAGG CC^T[ACGT]AGG
DrdI 319 GACTACGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 753 CCTGAGG CC^T[ACGT]AGG
FspI 1043 TGCGCA TGC^GCA
HincII 724 GTCAAC GT[CT]^[GA]AC
HindII 724 GTCAAC GT[CT]^[GA]AC
HinfI 814 GAGTC G^A[ACGT]TC
MamI 192 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1436 TCCGGA T^CCGGA
MslI 1257 CATCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 138 GCGGCCGC GC^GGCCGC
NspI 626 GCATGC [GA]CATG^[CT]
PflFI 1285 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 814 GAGTC GAGTC(4/5)
PvuII 1471 CAGCTG CAG^CTG
SacI 1405 GAGCTC GAGCT^C
ScaI 218 AGTACT AGT^ACT
SexAI 914 ACCTGGT A^CC[AT]GGT
SphI 626 GCATGC GCATG^C
StuI 278 AGGCCT AGG^CCT
Tsp509I 22 AATT ^AATT
Tth111I 1285 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1262 CCATGACGACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AlwNI 130 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1090 CAGACGCTG
BalI 920 TGGCCA TGG^CCA
1157 TGGCCA
BanI 867 GGCGCC G^G[CT][GA]CC
958 GGCGCC
BbeI 867 GGCGCC GGCGC^C
958 GGCGCC
BcgI 284 GCACCAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
677 GCAAGGTGTTCG
BlpI 45 GCTGAGC GC^T[ACGT]AGC
1473 GCTGAGC
BpmI 841 CTGGAG CTGGAG(16/14)
1402 CTGGAG
Bpu1102I 45 GCTGAGC GC^T[ACGT]AGC
1473 GCTGAGC
BsmAI 17 GAGAC GTCTC(1/5)
1529 GAGAC
BsmFI 1291 GTCCC GGGAC(10/14)
1482 GTCCC
BspMI 331 ACCTGC ACCTGC(4/8)
506 ACCTGC
CelII 45 GCTGAGC GC^T[ACGT]AGC
1473 GCTGAGC
DpnII 192 GATC ^GATC
909 GATC
DraII 364 GGGGCCT [GA]G^G[ACGT]CC[CT]
590 AGGGCCC
EagI 139 CGGCCG C^GGCCG
596 CGGCCG
EclXI 139 CGGCCG C^GGCCG
596 CGGCCG
Eco52I 139 CGGCCG C^GGCCG
596 CGGCCG
EcoNI 753 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1002 CCTGGACAAGG
EcoO109I 364 GGGGCCT [GA]G^G[ACGT]CC[CT]
590 AGGGCCC
KasI 867 GGCGCC G^GCGCC
958 GGCGCC
MboI 192 GATC ^GATC
909 GATC
MluNI 920 TGGCCA TGG^CCA
1157 TGGCCA
MscI 920 TGGCCA TGG^CCA
1157 TGGCCA
NarI 867 GGCGCC GG^CGCC
958 GGCGCC
NdeII 192 GATC ^GATC
909 GATC
PflMI 1074 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1311 CCAGGTAGTGG
PstI 508 CTGCAG CTGCA^G
1087 CTGCAG
SapI 702 GCTCTTC GCTCTTC(1/4)
1143 GCTCTTC
Sau3AI 192 GATC ^GATC
909 GATC
SfoI 867 GGCGCC GGC^GCC
958 GGCGCC
SmaI 360 CCCGGG CCC^GGG
1305 CCCGGG
SmlI 274 CTCAAG C^T[CT][GA]AG
1408 CTCAAG
TspRI 778 CACTG CA[GC]TG[ACGT][ACGT]^
1491 CAGTG
Van91I 1074 CCACTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1311 CCAGGTAGTGG
XmaCI 360 CCCGGG C^CCGGG
1305 CCCGGG
XmaI 360 CCCGGG C^CCGGG
1305 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BsrBI BsrI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI EaeI EarI
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI Ksp632I
MaeII MaeIII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI SfcI TaqI TseI Tsp45I
TthHB8I