(Circular) (Six-base) MAPSORT of: pFN21AE4125.seq Check: 7316 from: 1 to: 6244 pFN21AE4125 6244 bp With 182 enzymes: SgfI * March 28, 2012 13:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2345 2345 Size: 6244 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2334 4054 278 Size: 53 83 186 936 798 1720 2468 Fragments arranged by size: 2468 1720 936 798 186 83 53 AccI GT'mk_AC Cuts at: 1937 3587 1937 Size: 1650 4594 Acc65I G'GTAC_C Cuts at: 3565 3565 Size: 6244 AclI AA'CG_TT Cuts at: 1563 3543 4371 4744 1563 Size: 1980 828 373 3063 Fragments arranged by size: 3063 1980 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4307 5691 5959 1632 Size: 2675 1384 268 1917 Fragments arranged by size: 2675 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6047 AflIII A'CryG_T Cuts at: 1203 2313 5143 6120 1203 Size: 1110 2830 977 1327 Fragments arranged by size: 2830 1327 1110 977 AgeI A'CCGG_T Cuts at: 5075 5075 Size: 6244 AhdI GACnn_n'nnGTC Cuts at: 4973 4973 Size: 6244 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6244 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6244 AlwNI CAG_nnn'CTG Cuts at: 2140 3100 3987 5559 2140 Size: 960 887 1572 2825 Fragments arranged by size: 2825 1572 960 887 ApaI G_GGCC'C Cuts at: 2600 2600 Size: 6244 ApaLI G'TGCA_C Cuts at: 4301 5457 4301 Size: 1156 5088 ApoI r'AATT_y Cuts at: 1493 3553 3779 3931 6040 1493 Size: 2060 226 152 2109 1697 Fragments arranged by size: 2109 2060 1697 226 152 AscI GG'CGCG_CC Cuts at: 3074 3074 Size: 6244 AseI AT'TA_AT Cuts at: 160 1032 4798 160 Size: 872 3766 1606 Fragments arranged by size: 3766 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 AvaI C'yCGr_G Cuts at: 1870 1943 2177 2365 2668 3221 3310 3569 1870 Size: 73 234 188 303 553 89 259 4545 Fragments arranged by size: 4545 553 303 259 234 188 89 73 BamHI G'GATC_C Cuts at: 3574 3941 3574 Size: 367 5877 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2872 2963 3565 5020 618 Size: 325 217 627 1085 91 602 1455 1842 Fragments arranged by size: 1842 1455 1085 627 602 325 217 91 BanII G_rGCy'C Cuts at: 1391 2160 2600 2903 3108 3141 3414 3563 1391 Size: 769 440 303 205 33 273 149 4072 Fragments arranged by size: 4072 769 440 303 273 205 149 33 BbeI G_GCGC'C Cuts at: 2876 2967 2876 Size: 91 6153 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2800 928 Size: 971 901 4372 Fragments arranged by size: 4372 971 901 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2278 2312 2671 2705 4435 4469 1903 Size: 34 341 34 359 34 1730 34 3678 Fragments arranged by size: 3678 1730 359 341 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4136 5346 4136 Size: 1210 5034 BclI T'GATC_A Cuts at: 1548 1548 Size: 6244 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4855 136 Size: 107 122 71 631 605 3183 1525 Fragments arranged by size: 3183 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6239 6239 Size: 6244 BlpI GC'TnA_GC Cuts at: 2051 3479 3661 2051 Size: 1428 182 4634 Fragments arranged by size: 4634 1428 182 Bme1580I G_kGCm'C Cuts at: 1790 2600 4305 5461 1790 Size: 810 1705 1156 2573 Fragments arranged by size: 2573 1705 1156 810 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6244 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2473 3362 3975 Size: 416 53 83 399 24 1056 889 613 Cuts at: 3975 4933 442 Size: 958 1753 Fragments arranged by size: 1753 1056 958 889 613 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6244 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2867 3428 4904 1239 Size: 1628 561 1476 2579 Fragments arranged by size: 2579 1628 1476 561 Bpu10I CC'TnA_GC Cuts at: 3329 3341 3329 Size: 12 6232 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2264 3398 4369 5234 5532 2264 Size: 1134 971 865 298 2976 Fragments arranged by size: 2976 1134 971 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4907 882 Size: 617 3408 2219 Fragments arranged by size: 3408 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3061 4035 493 Size: 711 1857 974 2702 Fragments arranged by size: 2702 1857 974 711 BsaBI GATnn'nnATC Cuts at: 2201 6238 2201 Size: 4037 2207 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2331 2873 2964 Size: 53 83 186 936 534 264 542 91 Cuts at: 2964 4051 4433 5068 275 Size: 1087 382 635 1451 Fragments arranged by size: 1451 1087 936 635 542 534 382 264 186 91 83 53 BsaWI w'CCGG_w Cuts at: 3441 4677 5075 5349 5496 5907 3441 Size: 1236 398 274 147 411 3778 Fragments arranged by size: 3778 1236 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2444 2474 2810 2840 3377 Size: 30 165 30 1041 30 336 30 537 Cuts at: 3377 3407 1178 Size: 30 4015 Fragments arranged by size: 4015 1041 537 336 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2465 2540 2780 2783 2786 3511 1186 Size: 1279 75 240 3 3 725 3919 Fragments arranged by size: 3919 1279 725 240 75 3 3 BseYI C'CCAG_C Cuts at: 2753 2876 3100 5447 5943 2753 Size: 123 224 2347 496 3054 Fragments arranged by size: 3054 2347 496 224 123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2041 2272 3106 1163 Size: 573 305 231 834 4301 Fragments arranged by size: 4301 834 573 305 231 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2147 2359 2604 3694 4455 5483 Size: 275 186 146 212 245 1090 761 1028 Cuts at: 5483 1540 Size: 2301 Fragments arranged by size: 2301 1090 1028 761 275 245 212 186 146 BsiHKAI G_wGCw'C Cuts at: 2709 3308 3414 4305 4390 5461 2709 Size: 599 106 891 85 1071 3492 Fragments arranged by size: 3492 1071 891 599 106 85 BsmI GAATG_Cn' Cuts at: 3755 3848 3755 Size: 93 6151 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2600 2709 2903 3108 3141 3308 Size: 399 370 440 109 194 205 33 167 Cuts at: 3308 3414 3563 4305 4390 5461 1391 Size: 106 149 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 440 399 370 205 194 167 149 109 106 85 33 BspEI T'CCGG_A Cuts at: 3441 5907 3441 Size: 2466 3778 BspHI T'CATG_A Cuts at: 4133 5863 4133 Size: 1730 4514 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2345 2520 3599 844 Size: 1501 175 1079 3489 Fragments arranged by size: 3489 1501 1079 175 BsrBI CCG'CTC Cuts at: 1394 1823 2548 3345 3399 4131 1394 Size: 429 725 797 54 732 3507 Fragments arranged by size: 3507 797 732 725 429 54 BsrDI GCAATG_nn' Cuts at: 65 1248 4739 4913 65 Size: 1183 3491 174 1396 Fragments arranged by size: 3491 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4888 5075 1700 Size: 110 3078 187 2869 Fragments arranged by size: 3078 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6244 BssHII G'CGCG_C Cuts at: 1925 2046 2398 2433 3074 1925 Size: 121 352 35 641 5095 Fragments arranged by size: 5095 641 352 121 35 BssSI C'ACGA_G Cuts at: 4304 5316 4304 Size: 1012 5232 BstAPI GCAn_nnn'nTGC Cuts at: 794 2140 794 Size: 1346 4898 BstBI TT'CG_AA Cuts at: 5048 5048 Size: 6244 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6244 BstYI r'GATC_y Cuts at: 1972 3574 3941 4326 4343 5795 5881 5893 6239 Size: 1602 367 385 17 1452 86 12 346 Cuts at: 6239 1972 Size: 1977 Fragments arranged by size: 1977 1602 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2759 2759 Size: 6244 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5692 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2334 2455 3151 487 Size: 683 56 1108 121 696 3580 Fragments arranged by size: 3580 1108 696 683 121 56 BtsI GCAGTG_nn' Cuts at: 3760 4554 4574 3760 Size: 794 20 5430 Fragments arranged by size: 5430 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6244 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6209 DraI TTT'AAA Cuts at: 3548 3548 Size: 6244 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2330 5251 809 Size: 507 1014 2921 1802 Fragments arranged by size: 2921 1802 1014 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2144 2601 2925 Size: 54 1425 50 275 18 314 457 324 Cuts at: 2925 3162 3210 3691 4580 6209 8 Size: 237 48 481 889 1629 43 Fragments arranged by size: 1629 1425 889 481 457 324 314 275 237 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2144 2601 3691 1537 Size: 275 332 457 1090 4090 Fragments arranged by size: 4090 1090 457 332 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2550 2714 3155 3179 4174 1353 Size: 1197 164 441 24 995 3423 Fragments arranged by size: 3423 1197 995 441 164 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4831 5205 5351 387 Size: 1064 138 3242 374 146 1280 Fragments arranged by size: 3242 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3412 3412 Size: 6244 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2867 3428 4307 4904 5691 5959 1239 Size: 393 1235 561 879 597 787 268 1524 Fragments arranged by size: 1524 1235 879 787 597 561 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2762 3011 2762 Size: 249 5995 EcoO109I rG'GnC_Cy Cuts at: 2370 2596 2370 Size: 226 6018 EcoRI G'AATT_C Cuts at: 3553 3931 3553 Size: 378 5866 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2905 2905 Size: 6244 FspI TGC'GCA Cuts at: 3050 4750 3050 Size: 1700 4544 HaeII r_GCGC'y Cuts at: 1678 2101 2506 2629 2876 2967 3047 5391 1678 Size: 423 405 123 247 91 80 2344 2531 Fragments arranged by size: 2531 2344 423 405 247 123 91 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4885 4917 4959 4991 1373 Size: 32 135 32 3313 32 42 32 2626 Fragments arranged by size: 3313 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2731 3588 3834 669 Size: 988 281 793 857 246 3079 Fragments arranged by size: 3079 988 857 793 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6244 HpaI GTT'AAC Cuts at: 3834 3834 Size: 6244 KasI G'GCGC_C Cuts at: 2872 2963 2872 Size: 91 6153 KpnI G_GTAC'C Cuts at: 3569 3569 Size: 6244 MfeI C'AATT_G Cuts at: 3843 3843 Size: 6244 MluI A'CGCG_T Cuts at: 6120 6120 Size: 6244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2174 5358 5542 1305 Size: 869 3184 184 2007 Fragments arranged by size: 3184 2007 869 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2927 3164 10 Size: 54 1425 343 1095 237 3090 Fragments arranged by size: 3090 1425 1095 343 237 54 MslI CAynn'nnrTG Cuts at: 518 1166 3266 4202 4561 4720 518 Size: 648 2100 936 359 159 2042 Fragments arranged by size: 2100 2042 936 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2137 2260 2905 3120 3478 3660 4337 5485 Size: 531 123 645 215 358 182 677 1148 Cuts at: 5485 5730 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 645 531 358 245 215 182 123 NarI GG'CG_CC Cuts at: 2873 2964 2873 Size: 91 6153 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5692 NdeI CA'TA_TG Cuts at: 387 387 Size: 6244 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6244 NotI GC'GGCC_GC Cuts at: 2144 3691 2144 Size: 1547 4697 NspI r_CATG'y Cuts at: 1132 1229 2635 3602 5147 1132 Size: 97 1406 967 1545 2229 Fragments arranged by size: 2229 1545 1406 967 97 PasI CC'CwG_GG Cuts at: 1348 3085 1348 Size: 1737 4507 PciI A'CATG_T Cuts at: 5143 5143 Size: 6244 PflMI CCAn_nnn'nTGG Cuts at: 1108 3085 3322 1108 Size: 1977 237 4030 Fragments arranged by size: 4030 1977 237 PfoI T'CCnGG_A Cuts at: 2445 2445 Size: 6244 PmeI GTTT'AAAC Cuts at: 3548 3548 Size: 6244 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4313 4345 5826 5858 4313 Size: 32 1481 32 4699 Fragments arranged by size: 4699 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6244 PsiI TTA'TAA Cuts at: 3814 3814 Size: 6244 PspOMI G'GGCC_C Cuts at: 2596 2596 Size: 6244 PstI C_TGCA'G Cuts at: 830 2517 3096 3596 830 Size: 1687 579 500 3478 Fragments arranged by size: 3478 1687 579 500 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6244 PvuII CAG'CTG Cuts at: 3478 3478 Size: 6244 SacI G_AGCT'C Cuts at: 3414 3414 Size: 6244 SalI G'TCGA_C Cuts at: 1936 3586 1936 Size: 1650 4594 SapI GCTCTTCn'nnn_ Cuts at: 2714 3155 2714 Size: 441 5803 SbfI CC_TGCA'GG Cuts at: 3596 3596 Size: 6244 ScaI AGT'ACT Cuts at: 2225 4492 6204 2225 Size: 2267 1712 2265 Fragments arranged by size: 2267 2265 1712 SexAI A'CCwGG_T Cuts at: 2919 2919 Size: 6244 SfcI C'TryA_G Cuts at: 826 1045 2041 2513 3092 3592 4727 5408 5599 Size: 219 996 472 579 500 1135 681 191 Cuts at: 5599 826 Size: 1471 Fragments arranged by size: 1471 1135 996 681 579 500 472 219 191 SfoI GGC'GCC Cuts at: 2874 2965 2874 Size: 91 6153 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6244 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6244 SmaI CCC'GGG Cuts at: 1872 2367 3312 3571 1872 Size: 495 945 259 4545 Fragments arranged by size: 4545 945 495 259 SmlI C'TyrA_G Cuts at: 820 1017 1943 2279 3413 4348 5249 5511 820 Size: 197 926 336 1134 935 901 262 1553 Fragments arranged by size: 1553 1134 935 926 901 336 262 197 SnaBI TAC'GTA Cuts at: 493 4035 493 Size: 3542 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6244 SphI G_CATG'C Cuts at: 1132 2635 3602 1132 Size: 1503 967 3774 Fragments arranged by size: 3774 1503 967 SspI AAT'ATT Cuts at: 5 52 4168 5 Size: 47 4116 2081 Fragments arranged by size: 4116 2081 47 StuI AGG'CCT Cuts at: 1517 2285 1517 Size: 768 5476 StyI C'CwwG_G Cuts at: 513 1065 3683 513 Size: 552 2618 3074 Fragments arranged by size: 3074 2618 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4271 4288 4441 4626 5071 325 Size: 1255 2691 17 153 185 445 1498 Fragments arranged by size: 2691 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2223 2460 4490 Size: 274 80 33 51 1443 246 237 2030 Cuts at: 4490 6202 96 Size: 1712 138 Fragments arranged by size: 2030 1712 1443 274 246 237 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2810 2842 3377 3409 6204 6236 2810 Size: 32 535 32 2795 32 2818 Fragments arranged by size: 2818 2795 535 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3293 1316 Size: 1977 4267 XbaI T'CTAG_A Cuts at: 3580 3580 Size: 6244 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3274 3274 Size: 6244 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6244 XmaI C'CCGG_G Cuts at: 1870 2365 3310 3569 1870 Size: 495 945 259 4545 Fragments arranged by size: 4545 945 495 259 XmnI GAAnn'nnTTC Cuts at: 4373 4373 Size: 6244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2332 4052 276 Size: 53 83 186 936 798 1720 2468 Fragments arranged by size: 2468 1720 936 798 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI