(Linear) (Six-base) MAPSORT of: KIEE4125.seq Check: 2293 from: 1 to: 1539 KIEE4125.orf With 182 enzymes: SgfI * March 22, 2012 17:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 340 1539 Size: 340 1199 AatII G_ACGT'C Cuts at: 0 329 1539 Size: 329 1210 AflIII A'CryG_T Cuts at: 0 308 1539 Size: 308 1231 AlwNI CAG_nnn'CTG Cuts at: 0 135 1095 1539 Size: 135 960 444 Fragments arranged by size: 960 444 135 ApaI G_GGCC'C Cuts at: 0 595 1539 Size: 595 944 AscI GG'CGCG_CC Cuts at: 0 1069 1539 Size: 1069 470 AvaI C'yCGr_G Cuts at: 0 172 360 663 1216 1305 1539 Size: 172 188 303 553 89 234 Fragments arranged by size: 553 303 234 188 172 89 BanI G'GyrC_C Cuts at: 0 867 958 1539 Size: 867 91 581 Fragments arranged by size: 867 581 91 BanII G_rGCy'C Cuts at: 0 155 595 898 1103 1136 1409 1539 Size: 155 440 303 205 33 273 130 Fragments arranged by size: 440 303 273 205 155 130 33 BbeI G_GCGC'C Cuts at: 0 871 962 1539 Size: 871 91 577 Fragments arranged by size: 871 577 91 BbsI GAAGACnn'nnnn_ Cuts at: 0 795 1539 Size: 795 744 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 273 307 666 700 1539 Size: 273 34 359 34 839 Fragments arranged by size: 839 359 273 34 34 BlpI GC'TnA_GC Cuts at: 0 46 1474 1539 Size: 46 1428 65 Fragments arranged by size: 1428 65 46 Bme1580I G_kGCm'C Cuts at: 0 595 1539 Size: 595 944 BmrI ACTGGGnnnn_n' Cuts at: 0 468 1357 1539 Size: 468 889 182 Fragments arranged by size: 889 468 182 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 862 1423 1539 Size: 862 561 116 Fragments arranged by size: 862 561 116 Bpu10I CC'TnA_GC Cuts at: 0 1324 1336 1539 Size: 1324 12 203 Fragments arranged by size: 1324 203 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 259 1393 1539 Size: 259 1134 146 Fragments arranged by size: 1134 259 146 BsaAI yAC'GTr Cuts at: 0 1056 1539 Size: 1056 483 BsaBI GATnn'nnATC Cuts at: 0 196 1539 Size: 196 1343 BsaHI Gr'CG_yC Cuts at: 0 62 326 868 959 1539 Size: 62 264 542 91 580 Fragments arranged by size: 580 542 264 91 62 BsaWI w'CCGG_w Cuts at: 0 1436 1539 Size: 1436 103 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 439 469 805 835 1372 1402 1539 Size: 439 30 336 30 537 30 137 Fragments arranged by size: 537 439 336 137 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 460 535 775 778 781 1506 1539 Size: 460 75 240 3 3 725 33 Fragments arranged by size: 725 460 240 75 33 3 3 BseYI C'CCAG_C Cuts at: 0 748 871 1095 1539 Size: 748 123 224 444 Fragments arranged by size: 748 444 224 123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 267 1101 1539 Size: 36 231 834 438 Fragments arranged by size: 834 438 231 36 BsiEI CG_ry'CG Cuts at: 0 142 354 599 1539 Size: 142 212 245 940 Fragments arranged by size: 940 245 212 142 BsiHKAI G_wGCw'C Cuts at: 0 704 1303 1409 1539 Size: 704 599 106 130 Fragments arranged by size: 704 599 130 106 Bsp1286I G_dGCh'C Cuts at: 0 155 595 704 898 1103 1136 1303 1409 Size: 155 440 109 194 205 33 167 106 Cuts at: 1409 1539 Size: 130 Fragments arranged by size: 440 205 194 167 155 130 109 106 33 BspEI T'CCGG_A Cuts at: 0 1436 1539 Size: 1436 103 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 340 515 1539 Size: 340 175 1024 Fragments arranged by size: 1024 340 175 BsrBI CCG'CTC Cuts at: 0 543 1340 1394 1539 Size: 543 797 54 145 Fragments arranged by size: 797 543 145 54 BssHII G'CGCG_C Cuts at: 0 41 393 428 1069 1539 Size: 41 352 35 641 470 Fragments arranged by size: 641 470 352 41 35 BstAPI GCAn_nnn'nTGC Cuts at: 0 135 1539 Size: 135 1404 Bsu36I CC'TnA_GG Cuts at: 0 754 1539 Size: 754 785 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 329 450 1146 1539 Size: 329 121 696 393 Fragments arranged by size: 696 393 329 121 DrdI GACnn_nn'nnGTC Cuts at: 0 325 1539 Size: 325 1214 EaeI y'GGCC_r Cuts at: 0 139 596 920 1157 1205 1539 Size: 139 457 324 237 48 334 Fragments arranged by size: 457 334 324 237 139 48 EagI C'GGCC_G Cuts at: 0 139 596 1539 Size: 139 457 943 Fragments arranged by size: 943 457 139 EarI CTCTTCn'nnn_ Cuts at: 0 545 709 1150 1174 1539 Size: 545 164 441 24 365 Fragments arranged by size: 545 441 365 164 24 EcoICRI GAG'CTC Cuts at: 0 1407 1539 Size: 1407 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 862 1423 1539 Size: 862 561 116 Fragments arranged by size: 862 561 116 EcoNI CCTnn'n_nnAGG Cuts at: 0 757 1006 1539 Size: 757 249 533 Fragments arranged by size: 757 533 249 EcoO109I rG'GnC_Cy Cuts at: 0 365 591 1539 Size: 365 226 948 Fragments arranged by size: 948 365 226 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 900 1539 Size: 900 639 FspI TGC'GCA Cuts at: 0 1045 1539 Size: 1045 494 HaeII r_GCGC'y Cuts at: 0 96 501 624 871 962 1042 1539 Size: 96 405 123 247 91 80 497 Fragments arranged by size: 497 405 247 123 96 91 80 HincII GTy'rAC Cuts at: 0 726 1539 Size: 726 813 KasI G'GCGC_C Cuts at: 0 867 958 1539 Size: 867 91 581 Fragments arranged by size: 867 581 91 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 169 1539 Size: 169 1370 MscI TGG'CCA Cuts at: 0 922 1159 1539 Size: 922 237 380 Fragments arranged by size: 922 380 237 MslI CAynn'nnrTG Cuts at: 0 1261 1539 Size: 1261 278 MspA1I CmG'CkG Cuts at: 0 132 255 900 1115 1473 1539 Size: 132 123 645 215 358 66 Fragments arranged by size: 645 358 215 132 123 66 NarI GG'CG_CC Cuts at: 0 868 959 1539 Size: 868 91 580 Fragments arranged by size: 868 580 91 NotI GC'GGCC_GC Cuts at: 0 139 1539 Size: 139 1400 NspI r_CATG'y Cuts at: 0 630 1539 Size: 630 909 PasI CC'CwG_GG Cuts at: 0 1080 1539 Size: 1080 459 PflMI CCAn_nnn'nTGG Cuts at: 0 1080 1317 1539 Size: 1080 237 222 Fragments arranged by size: 1080 237 222 PfoI T'CCnGG_A Cuts at: 0 440 1539 Size: 440 1099 PspOMI G'GGCC_C Cuts at: 0 591 1539 Size: 591 948 PstI C_TGCA'G Cuts at: 0 512 1091 1539 Size: 512 579 448 Fragments arranged by size: 579 512 448 PvuII CAG'CTG Cuts at: 0 1473 1539 Size: 1473 66 SacI G_AGCT'C Cuts at: 0 1409 1539 Size: 1409 130 SapI GCTCTTCn'nnn_ Cuts at: 0 709 1150 1539 Size: 709 441 389 Fragments arranged by size: 709 441 389 ScaI AGT'ACT Cuts at: 0 220 1539 Size: 220 1319 SexAI A'CCwGG_T Cuts at: 0 914 1539 Size: 914 625 SfcI C'TryA_G Cuts at: 0 36 508 1087 1539 Size: 36 472 579 452 Fragments arranged by size: 579 472 452 36 SfoI GGC'GCC Cuts at: 0 869 960 1539 Size: 869 91 579 Fragments arranged by size: 869 579 91 SmaI CCC'GGG Cuts at: 0 362 1307 1539 Size: 362 945 232 Fragments arranged by size: 945 362 232 SmlI C'TyrA_G Cuts at: 0 274 1408 1539 Size: 274 1134 131 Fragments arranged by size: 1134 274 131 SphI G_CATG'C Cuts at: 0 630 1539 Size: 630 909 StuI AGG'CCT Cuts at: 0 280 1539 Size: 280 1259 TatI w'GTAC_w Cuts at: 0 218 455 1539 Size: 218 237 1084 Fragments arranged by size: 1084 237 218 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 805 837 1372 1404 1539 Size: 805 32 535 32 135 Fragments arranged by size: 805 535 135 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1288 1539 Size: 1288 251 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1269 1539 Size: 1269 270 XmaI C'CCGG_G Cuts at: 0 360 1305 1539 Size: 360 945 234 Fragments arranged by size: 945 360 234 ZraI GAC'GTC Cuts at: 0 327 1539 Size: 327 1212 Enzymes that do cut: AarI AatII AflIII AlwNI ApaI AscI AvaI BanI BanII BbeI BbsI BcgI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BssHII BstAPI Bsu36I BtgZI DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII HincII KasI MmeI MscI MslI MspA1I NarI NotI NspI PasI PflMI PfoI PspOMI PstI PvuII SacI SapI ScaI SexAI SfcI SfoI SmaI SmlI SphI StuI TatI TstI Tth111I XcmI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglI BglII BmgBI BmtI BplI BsaI BsiWI BsmI BsmBI BspHI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I BtgI BtsI ClaI CspCI DraI DraIII EciI EcoRI EcoRV FseI FspAI Hin4I HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI StyI SwaI TaqII XbaI XhoI XmnI