################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbrPI BbsI BciVI BclI BfrI BlnI BpuAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 475 GGTACC G^GTACC Asp718I 475 GGTACC G^GTACC BalI 1563 TGGCCA TGG^CCA BamHI 796 GGATCC G^GATCC BfaI 901 CTAG C^TAG BlpI 123 GCTAAGC GC^T[ACGT]AGC Bpu1102I 123 GCTAAGC GC^T[ACGT]AGC BsaAI 364 TACGTG [CT]AC^GT[GA] BsaI 1142 GAGACC GGTCTC(1/5) BsmBI 1542 GAGACG CGTCTC(1/5) BsmFI 408 GTCCC GGGAC(10/14) BspLU11I 497 ACATGT A^CATGT BspMI 519 ACCTGC ACCTGC(4/8) BsrBI 1202 CCGCTC CCGCTC(-3/-3) BstAPI 1212 GCATGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 123 GCTAAGC GC^T[ACGT]AGC KpnI 475 GGTACC GGTAC^C MaeI 901 CTAG C^TAG MaeIII 1288 GTGAC ^GT[ACGT]AC MluNI 1563 TGGCCA TGG^CCA MscI 1563 TGGCCA TGG^CCA NheI 900 GCTAGC G^CTAGC PflMI 1506 CCAGTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 587 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1452 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 1173 GAGCTC GAGCT^C SmaI 630 CCCGGG CCC^GGG SphI 1212 GCATGC GCATG^C Tsp45I 1288 GTGAC ^GT[GC]AC Van91I 1506 CCAGTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 630 CCCGGG C^CCGGG XmaI 630 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AccII 313 CGCG CG^CG 673 CGCG AflIII 365 ACGTGT A^C[GA][CT]GT 497 ACATGT AlwI 797 GATCC GGATC(4/5) 796 GGATC ApaI 627 GGGCCC GGGCC^C 1189 GGGCCC AspHI 715 GTGCTC G[AT]GC[AT]^C 1173 GAGCTC BcgI 1605 CGAGATCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 938 GCATAGGCCTCG BglI 805 GCCGTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 913 GCCCTCATGGC BsgI 515 CTGCAC GTGCAG(16/14) 878 GTGCAG BsiHKAI 715 GTGCTC G[AT]GC[AT]^C 1173 GAGCTC BstUI 313 CGCG CG^CG 673 CGCG BstXI 860 CCATGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1285 CCAGTGACCTGG EaeI 1370 CGGCCA [CT]^GGCC[GA] 1563 TGGCCA EcoNI 411 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1038 CCTGCTGCAGG FauI 1201 CCCGC CCCGC(4/6) 393 GCGGG MaeII 365 ACGT A^CGT 746 ACGT MslI 426 CATCACCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1235 CACTGCTGTG MspA1I 391 CAGCGG C[AC]G^C[GT]G 1454 CCGCTG MvnI 313 CGCG CG^CG 673 CGCG PstI 106 CTGCAG CTGCA^G 1042 CTGCAG SapI 1334 GAAGAGC GCTCTTC(1/4) 717 GCTCTTC SfcI 106 CTGCAG C^T[GA][CT]AG 1042 CTGCAG StuI 942 AGGCCT AGG^CCT 1017 AGGCCT TaqI 1400 TCGA T^CGA 1604 TCGA TthHB8I 1400 TCGA T^CGA 1604 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AciI AfaI AluI AvaI AvaII BanI BanII BbvCI BbvI BcnI BglII BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MboI MboII MflI MnlI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI TspRI XcmI XhoII