Restriction map for
pFN21AE6464
(Circular) (Six-base) MAPSORT of: pFN21AE6464.seq Check: 2125 from: 1 to: 6328
pFN21AE6464 6328 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2533 2533
Size: 6328
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4138 278
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3671 1937
Size: 1734 4594
Acc65I G'GTAC_C
Cuts at: 2480 3649 2480
Size: 1169 5159
AclI AA'CG_TT
Cuts at: 1563 4455 4828 1563
Size: 2892 373 3063
Fragments arranged by size:
3063 2892 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2653 2761 4391 5775 6043 1632
Size: 1021 108 1630 1384 268 1917
Fragments arranged by size:
1917 1630 1384 1021 268 108
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6131
AflIII A'CryG_T
Cuts at: 1203 2370 2502 5227 6204 1203
Size: 1167 132 2725 977 1327
Fragments arranged by size:
2725 1327 1167 977 132
AgeI A'CCGG_T
Cuts at: 5159 5159
Size: 6328
AhdI GACnn_n'nnGTC
Cuts at: 5057 5057
Size: 6328
AleI CACnn'nnGTG
Cuts at: 1166 3244 1166
Size: 2078 4250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2270 3262 1184
Size: 1086 992 4250
Fragments arranged by size:
4250 1086 992
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2132 2164 2132
Size: 32 6296
AlwNI CAG_nnn'CTG
Cuts at: 4071 5643 4071
Size: 1572 4756
ApaI G_GGCC'C
Cuts at: 2636 3198 2636
Size: 562 5766
ApaLI G'TGCA_C
Cuts at: 4385 5541 4385
Size: 1156 5172
ApoI r'AATT_y
Cuts at: 1493 3637 3863 4015 6124 1493
Size: 2144 226 152 2109 1697
Fragments arranged by size:
2144 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4882 160
Size: 872 3850 1606
Fragments arranged by size:
3850 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
AvaI C'yCGr_G
Cuts at: 1870 1943 2240 2635 3210 3653 1870
Size: 73 297 395 575 443 4545
Fragments arranged by size:
4545 575 443 395 297 73
BamHI G'GATC_C
Cuts at: 2801 3658 4025 2801
Size: 857 367 5104
Fragments arranged by size:
5104 857 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2480 3416 3649 5104
Size: 325 217 627 309 384 936 233 1455
Cuts at: 5104 618
Size: 1842
Fragments arranged by size:
1842 1455 936 627 384 325 309 233
217
BanII G_rGCy'C
Cuts at: 1391 2179 2636 2899 3182 3198 3647 1391
Size: 788 457 263 283 16 449 4072
Fragments arranged by size:
4072 788 457 449 283 263 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5357
BbvCI CC'TCA_GC
Cuts at: 2543 2806 3154 3308 2543
Size: 263 348 154 5563
Fragments arranged by size:
5563 348 263 154
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2932 2966 3599 3633 4519 4553 1903
Size: 34 995 34 633 34 886 34 3678
Fragments arranged by size:
3678 995 886 633 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4220 5430 4220
Size: 1210 5118
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6328
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2816 2924 4939
Size: 107 122 71 631 605 1144 108 2015
Cuts at: 4939 136
Size: 1525
Fragments arranged by size:
2015 1525 1144 631 605 122 108 107
71
BglII A'GATC_T
Cuts at: 3140 3315 3612 6323 3140
Size: 175 297 2711 3145
Fragments arranged by size:
3145 2711 297 175
BlpI GC'TnA_GC
Cuts at: 2129 3745 2129
Size: 1616 4712
Bme1580I G_kGCm'C
Cuts at: 1790 2101 2636 2677 2920 2970 3003 3198 4389
Size: 311 535 41 243 50 33 195 1191
Cuts at: 4389 5545 1790
Size: 1156 2573
Fragments arranged by size:
2573 1191 1156 535 311 243 195 50
41 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3379 4059 5017
Size: 416 53 83 399 24 1962 680 958
Cuts at: 5017 442
Size: 1753
Fragments arranged by size:
1962 1753 958 680 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2909 1056
Size: 1853 4475
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2591 3051 3165 4988 1239
Size: 1352 460 114 1823 2579
Fragments arranged by size:
2579 1823 1352 460 114
Bpu10I CC'TnA_GC
Cuts at: 2543 2806 3154 3176 3308 3560 2543
Size: 263 348 22 132 252 5311
Fragments arranged by size:
5311 348 263 252 132 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4453 5318 5616 4453
Size: 865 298 5165
Fragments arranged by size:
5165 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3141 4991 882
Size: 617 1642 1850 2219
Fragments arranged by size:
2219 1850 1642 617
BsaAI yAC'GTr
Cuts at: 493 1204 2371 4119 493
Size: 711 1167 1748 2702
Fragments arranged by size:
2702 1748 1167 711
BsaBI GATnn'nnATC
Cuts at: 6322 6322
Size: 6328
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4135 4517 5152 275
Size: 53 83 186 936 2602 382 635 1451
Fragments arranged by size:
2602 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4761 5159 5433 5580 5991 4761
Size: 398 274 147 411 5098
Fragments arranged by size:
5098 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2132 2162 2394 2424 1178
Size: 30 165 30 729 30 232 30 5082
Fragments arranged by size:
5082 729 232 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2171 2338 2540 3046 3153 1186
Size: 985 167 202 506 107 4361
Fragments arranged by size:
4361 985 506 202 167 107
BseYI C'CCAG_C
Cuts at: 2053 2511 2629 5531 6027 2053
Size: 458 118 2902 496 2354
Fragments arranged by size:
2902 2354 496 458 118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2505 2904 1163
Size: 573 769 399 4587
Fragments arranged by size:
4587 769 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3778 4539 5567 1540
Size: 275 186 1777 761 1028 2301
Fragments arranged by size:
2301 1777 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2724 3182 4389 4474 5545 2724
Size: 458 1207 85 1071 3507
Fragments arranged by size:
3507 1207 1071 458 85
BsmI GAATG_Cn'
Cuts at: 3839 3932 3839
Size: 93 6235
BsmBI CGTCTCn'nnnn_
Cuts at: 3541 3541
Size: 6328
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2179 2636 2677 2724 2899 2920
Size: 399 311 78 457 41 47 175 21
Cuts at: 2920 2970 3003 3182 3198 3647 4389 4474 5545
Size: 50 33 179 16 449 742 85 1071
Cuts at: 5545 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 457 449 399 311 179
175 85 78 50 47 41 33 21
16
BspEI T'CCGG_A
Cuts at: 5991 5991
Size: 6328
BspHI T'CATG_A
Cuts at: 4217 5947 4217
Size: 1730 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2533 3683 844
Size: 1689 1150 3489
Fragments arranged by size:
3489 1689 1150
BsrBI CCG'CTC
Cuts at: 1394 1823 3209 4215 1394
Size: 429 1386 1006 3507
Fragments arranged by size:
3507 1386 1006 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4823 4997 65
Size: 1183 3575 174 1396
Fragments arranged by size:
3575 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4972 5159 1700
Size: 110 3162 187 2869
Fragments arranged by size:
3162 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6328
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6328
BssSI C'ACGA_G
Cuts at: 4388 5400 4388
Size: 1012 5316
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3223 794
Size: 2429 3899
BstBI TT'CG_AA
Cuts at: 5132 5132
Size: 6328
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6328
BstXI CCAn_nnnn'nTGG
Cuts at: 2872 3297 2872
Size: 425 5903
BstYI r'GATC_y
Cuts at: 1972 2801 3140 3315 3612 3658 4025 4410 4427
Size: 829 339 175 297 46 367 385 17
Cuts at: 4427 5879 5965 5977 6323 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 829 385 367 346 339 297
175 86 46 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2352 2390 2436 2645 2794 2865
Size: 552 939 348 38 46 209 149 71
Cuts at: 2865 2970 513
Size: 105 3871
Fragments arranged by size:
3871 939 552 348 209 149 105 71
46 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5589
Fragments arranged by size:
5589 683 56
BtsI GCAGTG_nn'
Cuts at: 2108 2517 2844 3111 3221 3239 3844 4638 4658
Size: 409 327 267 110 18 605 794 20
Cuts at: 4658 2108
Size: 3778
Fragments arranged by size:
3778 794 605 409 327 267 110 20
18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6328
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6293
DraI TTT'AAA
Cuts at: 3632 3632
Size: 6328
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5335 809
Size: 507 4019 1802
Fragments arranged by size:
4019 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3375 3568 3775
Size: 54 1425 50 275 18 1545 193 207
Cuts at: 3775 4664 6293 8
Size: 889 1629 43
Fragments arranged by size:
1629 1545 1425 889 275 207 193 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3775 1537
Size: 275 1963 4090
Fragments arranged by size:
4090 1963 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2558 2672 2729 3334 3389 3539 4258 1353
Size: 1205 114 57 605 55 150 719 3423
Fragments arranged by size:
3423 1205 719 605 150 114 57 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4915 5289 5435 387
Size: 1064 138 3326 374 146 1280
Fragments arranged by size:
3326 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3180 3180
Size: 6328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2591 2653 2761 3051 3165 4391 4988
Size: 393 959 62 108 290 114 1226 597
Cuts at: 4988 5775 6043 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1226 959 787 597 393 290 268
114 108 62
EcoNI CCTnn'n_nnAGG
Cuts at: 2420 3047 2420
Size: 627 5701
EcoO109I rG'GnC_Cy
Cuts at: 2593 2620 3195 2593
Size: 27 575 5726
Fragments arranged by size:
5726 575 27
EcoRI G'AATT_C
Cuts at: 3637 4015 3637
Size: 378 5950
FspI TGC'GCA
Cuts at: 4834 4834
Size: 6328
HaeII r_GCGC'y
Cuts at: 1678 5475 1678
Size: 3797 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4969 5001 5043 5075 1373
Size: 32 135 32 3397 32 42 32 2626
Fragments arranged by size:
3397 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3672 3918 669
Size: 988 281 1734 246 3079
Fragments arranged by size:
3079 1734 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6328
HpaI GTT'AAC
Cuts at: 3918 3918
Size: 6328
KpnI G_GTAC'C
Cuts at: 2484 3653 2484
Size: 1169 5159
MfeI C'AATT_G
Cuts at: 3927 3927
Size: 6328
MluI A'CGCG_T
Cuts at: 6204 6204
Size: 6328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5442 5626 1305
Size: 4137 184 2007
Fragments arranged by size:
4137 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3570 10
Size: 54 1425 343 1738 2768
Fragments arranged by size:
2768 1738 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2435 3244 4286 4645 4804 518
Size: 648 1269 809 1042 359 159 2042
Fragments arranged by size:
2042 1269 1042 809 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2398 3461 3744 4421 5569 5814 1606
Size: 792 1063 283 677 1148 245 2120
Fragments arranged by size:
2120 1148 1063 792 677 283 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2352 2794 2865 2970 513
Size: 552 939 348 442 71 105 3871
Fragments arranged by size:
3871 939 552 442 348 105 71
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6328
NheI G'CTAG_C
Cuts at: 1052 2905 1052
Size: 1853 4475
NotI GC'GGCC_GC
Cuts at: 3775 3775
Size: 6328
NspI r_CATG'y
Cuts at: 1132 1229 2506 2689 3221 3686 5231 1132
Size: 97 1277 183 532 465 1545 2229
Fragments arranged by size:
2229 1545 1277 532 465 183 97
PasI CC'CwG_GG
Cuts at: 1348 2278 2487 3191 1348
Size: 930 209 704 4485
Fragments arranged by size:
4485 930 704 209
PciI A'CATG_T
Cuts at: 2502 5227 2502
Size: 2725 3603
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3517 1108
Size: 2409 3919
PfoI T'CCnGG_A
Cuts at: 3431 3431
Size: 6328
PmeI GTTT'AAAC
Cuts at: 3632 3632
Size: 6328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2132 2164 4397 4429 5910 5942 2132
Size: 32 2233 32 1481 32 2518
Fragments arranged by size:
2518 2233 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2593 2593
Size: 6328
PshAI GACnn'nnGTC
Cuts at: 1613 3461 1613
Size: 1848 4480
PsiI TTA'TAA
Cuts at: 3898 3898
Size: 6328
PspOMI G'GGCC_C
Cuts at: 2632 3194 2632
Size: 562 5766
PstI C_TGCA'G
Cuts at: 830 2115 3051 3680 830
Size: 1285 936 629 3478
Fragments arranged by size:
3478 1285 936 629
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6328
SacI G_AGCT'C
Cuts at: 3182 3182
Size: 6328
SalI G'TCGA_C
Cuts at: 1936 3670 1936
Size: 1734 4594
SapI GCTCTTCn'nnn_
Cuts at: 2729 3334 2729
Size: 605 5723
SbfI CC_TGCA'GG
Cuts at: 3680 3680
Size: 6328
ScaI AGT'ACT
Cuts at: 4576 6288 4576
Size: 1712 4616
SfcI C'TryA_G
Cuts at: 826 1045 2111 3047 3676 4811 5492 5683 826
Size: 219 1066 936 629 1135 681 191 1471
Fragments arranged by size:
1471 1135 1066 936 681 629 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6328
SmaI CCC'GGG
Cuts at: 1872 2637 3655 1872
Size: 765 1018 4545
Fragments arranged by size:
4545 1018 765
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4432 5333 5595 820
Size: 197 926 2489 901 262 1553
Fragments arranged by size:
2489 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4119 493
Size: 3626 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6328
SphI G_CATG'C
Cuts at: 1132 3221 3686 1132
Size: 2089 465 3774
Fragments arranged by size:
3774 2089 465
SspI AAT'ATT
Cuts at: 5 52 4252 5
Size: 47 4200 2081
Fragments arranged by size:
4200 2081 47
StuI AGG'CCT
Cuts at: 1517 2949 3024 1517
Size: 1432 75 4821
Fragments arranged by size:
4821 1432 75
StyI C'CwwG_G
Cuts at: 513 1065 2004 2352 2794 2865 2970 3074 3767
Size: 552 939 348 442 71 105 104 693
Cuts at: 3767 513
Size: 3074
Fragments arranged by size:
3074 939 693 552 442 348 105 104
71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 4355 4372 4525 4710 5155 325
Size: 1255 465 2310 17 153 185 445 1498
Fragments arranged by size:
2310 1498 1255 465 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4574 6286 96
Size: 274 80 33 51 1443 2597 1712 138
Fragments arranged by size:
2597 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2394 2426 2514 2546 6288 6320 2394
Size: 32 88 32 3742 32 2402
Fragments arranged by size:
3742 2402 88 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6328
XbaI T'CTAG_A
Cuts at: 3664 3664
Size: 6328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2707 2872 3140 2707
Size: 165 268 5895
Fragments arranged by size:
5895 268 165
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6328
XmaI C'CCGG_G
Cuts at: 1870 2635 3653 1870
Size: 765 1018 4545
Fragments arranged by size:
4545 1018 765
XmnI GAAnn'nnTTC
Cuts at: 4457 4457
Size: 6328
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4136 276
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI