(Linear) (Six-base) MAPSORT of: KIEE6464.seq Check: 9892 from: 1 to: 1623 KIEE6464.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 528 1623 Size: 528 1095 Acc65I G'GTAC_C Cuts at: 0 475 1623 Size: 475 1148 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 648 756 1623 Size: 648 108 867 Fragments arranged by size: 867 648 108 AflIII A'CryG_T Cuts at: 0 365 497 1623 Size: 365 132 1126 Fragments arranged by size: 1126 365 132 AleI CACnn'nnGTG Cuts at: 0 1239 1623 Size: 1239 384 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 265 1257 1623 Size: 265 992 366 Fragments arranged by size: 992 366 265 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 127 159 1623 Size: 127 32 1464 Fragments arranged by size: 1464 127 32 ApaI G_GGCC'C Cuts at: 0 631 1193 1623 Size: 631 562 430 Fragments arranged by size: 631 562 430 AvaI C'yCGr_G Cuts at: 0 235 630 1205 1623 Size: 235 395 575 418 Fragments arranged by size: 575 418 395 235 BamHI G'GATC_C Cuts at: 0 796 1623 Size: 796 827 BanI G'GyrC_C Cuts at: 0 91 475 1411 1623 Size: 91 384 936 212 Fragments arranged by size: 936 384 212 91 BanII G_rGCy'C Cuts at: 0 174 631 894 1177 1193 1623 Size: 174 457 263 283 16 430 Fragments arranged by size: 457 430 283 263 174 16 BbvCI CC'TCA_GC Cuts at: 0 538 801 1149 1303 1623 Size: 538 263 348 154 320 Fragments arranged by size: 538 348 320 263 154 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 927 961 1594 1623 Size: 927 34 633 29 Fragments arranged by size: 927 633 34 29 BglI GCCn_nnn'nGGC Cuts at: 0 811 919 1623 Size: 811 108 704 Fragments arranged by size: 811 704 108 BglII A'GATC_T Cuts at: 0 1135 1310 1607 1623 Size: 1135 175 297 16 Fragments arranged by size: 1135 297 175 16 BlpI GC'TnA_GC Cuts at: 0 124 1623 Size: 124 1499 Bme1580I G_kGCm'C Cuts at: 0 96 631 672 915 965 998 1193 1623 Size: 96 535 41 243 50 33 195 430 Fragments arranged by size: 535 430 243 195 96 50 41 33 BmrI ACTGGGnnnn_n' Cuts at: 0 1374 1623 Size: 1374 249 BmtI G_CTAG'C Cuts at: 0 904 1623 Size: 904 719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 586 1046 1160 1623 Size: 586 460 114 463 Fragments arranged by size: 586 463 460 114 Bpu10I CC'TnA_GC Cuts at: 0 538 801 1149 1171 1303 1555 1623 Size: 538 263 348 22 132 252 68 Fragments arranged by size: 538 348 263 252 132 68 22 BsaI GGTCTCn'nnnn_ Cuts at: 0 1136 1623 Size: 1136 487 BsaAI yAC'GTr Cuts at: 0 366 1623 Size: 366 1257 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 127 157 389 419 1623 Size: 127 30 232 30 1204 Fragments arranged by size: 1204 232 127 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 166 333 535 1041 1148 1623 Size: 166 167 202 506 107 475 Fragments arranged by size: 506 475 202 167 166 107 BseYI C'CCAG_C Cuts at: 0 48 506 624 1623 Size: 48 458 118 999 Fragments arranged by size: 999 458 118 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 500 899 1623 Size: 500 399 724 Fragments arranged by size: 724 500 399 BsiHKAI G_wGCw'C Cuts at: 0 719 1177 1623 Size: 719 458 446 Fragments arranged by size: 719 458 446 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1536 1623 Size: 1536 87 Bsp1286I G_dGCh'C Cuts at: 0 96 174 631 672 719 894 915 965 Size: 96 78 457 41 47 175 21 50 Cuts at: 965 998 1177 1193 1623 Size: 33 179 16 430 Fragments arranged by size: 457 430 179 175 96 78 50 47 41 33 21 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 528 1623 Size: 528 1095 BsrBI CCG'CTC Cuts at: 0 1204 1623 Size: 1204 419 BstAPI GCAn_nnn'nTGC Cuts at: 0 1218 1623 Size: 1218 405 BstXI CCAn_nnnn'nTGG Cuts at: 0 867 1292 1623 Size: 867 425 331 Fragments arranged by size: 867 425 331 BstYI r'GATC_y Cuts at: 0 796 1135 1310 1607 1623 Size: 796 339 175 297 16 Fragments arranged by size: 796 339 297 175 16 BtgI C'CryG_G Cuts at: 0 347 385 431 640 789 860 965 1623 Size: 347 38 46 209 149 71 105 658 Fragments arranged by size: 658 347 209 149 105 71 46 38 BtsI GCAGTG_nn' Cuts at: 0 103 512 839 1106 1216 1234 1623 Size: 103 409 327 267 110 18 389 Fragments arranged by size: 409 389 327 267 110 103 18 EaeI y'GGCC_r Cuts at: 0 1370 1563 1623 Size: 1370 193 60 Fragments arranged by size: 1370 193 60 EarI CTCTTCn'nnn_ Cuts at: 0 553 667 724 1329 1384 1534 1623 Size: 553 114 57 605 55 150 89 Fragments arranged by size: 605 553 150 114 89 57 55 EcoICRI GAG'CTC Cuts at: 0 1175 1623 Size: 1175 448 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 586 648 756 1046 1160 1623 Size: 586 62 108 290 114 463 Fragments arranged by size: 586 463 290 114 108 62 EcoNI CCTnn'n_nnAGG Cuts at: 0 415 1042 1623 Size: 415 627 581 Fragments arranged by size: 627 581 415 EcoO109I rG'GnC_Cy Cuts at: 0 588 615 1190 1623 Size: 588 27 575 433 Fragments arranged by size: 588 575 433 27 KpnI G_GTAC'C Cuts at: 0 479 1623 Size: 479 1144 MscI TGG'CCA Cuts at: 0 1565 1623 Size: 1565 58 MslI CAynn'nnrTG Cuts at: 0 430 1239 1623 Size: 430 809 384 Fragments arranged by size: 809 430 384 MspA1I CmG'CkG Cuts at: 0 393 1456 1623 Size: 393 1063 167 Fragments arranged by size: 1063 393 167 NcoI C'CATG_G Cuts at: 0 347 789 860 965 1623 Size: 347 442 71 105 658 Fragments arranged by size: 658 442 347 105 71 NheI G'CTAG_C Cuts at: 0 900 1623 Size: 900 723 NspI r_CATG'y Cuts at: 0 501 684 1216 1623 Size: 501 183 532 407 Fragments arranged by size: 532 501 407 183 PasI CC'CwG_GG Cuts at: 0 273 482 1186 1623 Size: 273 209 704 437 Fragments arranged by size: 704 437 273 209 PciI A'CATG_T Cuts at: 0 497 1623 Size: 497 1126 PflMI CCAn_nnn'nTGG Cuts at: 0 1512 1623 Size: 1512 111 PfoI T'CCnGG_A Cuts at: 0 1426 1623 Size: 1426 197 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 127 159 1623 Size: 127 32 1464 Fragments arranged by size: 1464 127 32 PpuMI rG'GwC_Cy Cuts at: 0 588 1623 Size: 588 1035 PshAI GACnn'nnGTC Cuts at: 0 1456 1623 Size: 1456 167 PspOMI G'GGCC_C Cuts at: 0 627 1189 1623 Size: 627 562 434 Fragments arranged by size: 627 562 434 PstI C_TGCA'G Cuts at: 0 110 1046 1623 Size: 110 936 577 Fragments arranged by size: 936 577 110 SacI G_AGCT'C Cuts at: 0 1177 1623 Size: 1177 446 SapI GCTCTTCn'nnn_ Cuts at: 0 724 1329 1623 Size: 724 605 294 Fragments arranged by size: 724 605 294 SfcI C'TryA_G Cuts at: 0 106 1042 1623 Size: 106 936 581 Fragments arranged by size: 936 581 106 SmaI CCC'GGG Cuts at: 0 632 1623 Size: 632 991 SphI G_CATG'C Cuts at: 0 1216 1623 Size: 1216 407 StuI AGG'CCT Cuts at: 0 944 1019 1623 Size: 944 75 604 Fragments arranged by size: 944 604 75 StyI C'CwwG_G Cuts at: 0 347 789 860 965 1069 1623 Size: 347 442 71 105 104 554 Fragments arranged by size: 554 442 347 105 104 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 40 1623 Size: 40 1583 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 389 421 509 541 1623 Size: 389 32 88 32 1082 Fragments arranged by size: 1082 389 88 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 702 867 1135 1623 Size: 702 165 268 488 Fragments arranged by size: 702 488 268 165 XmaI C'CCGG_G Cuts at: 0 630 1623 Size: 630 993 Enzymes that do cut: AarI Acc65I AcuI AflIII AleI AlfI AloI ApaI AvaI BamHI BanI BanII BbvCI BcgI BglI BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BstAPI BstXI BstYI BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I KpnI MscI MslI MspA1I NcoI NheI NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI SacI SapI SfcI SmaI SphI StuI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BciVI BclI BfrBI BmgBI BplI BpuEI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PmeI PmlI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmnI ZraI