################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I MaeIII MamI MfeI MluI MluNI MroI MscI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 29 CGCG CG^CG AcyI 16 GACGCC G[GA]^CG[CT]C AfaI 264 GTAC GT^AC AlwNI 182 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 650 CTCGAG C^[CT]CG[GA]G BanI 679 GGCACC G^G[CT][GA]CC BbsI 253 GTCTTC GAAGAC(2/6) BfaI 871 CTAG C^TAG BglI 587 GCCGGGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 757 GCTGAGC GC^T[ACGT]AGC BpmI 364 CTGGAG CTGGAG(16/14) Bpu1102I 757 GCTGAGC GC^T[ACGT]AGC BpuAI 253 GTCTTC GAAGAC(2/6) BsaHI 16 GACGCC G[GA]^CG[CT]C BsgI 139 GTGCAG GTGCAG(16/14) BsiEI 534 CGACCG CG[GA][CT]^CG BsoBI 650 CTCGAG C^[CT]CG[GA]G BspMI 135 GCAGGT ACCTGC(4/8) BsrBI 156 CCGCTC CCGCTC(-3/-3) BstAPI 765 GCATCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 29 CGCG CG^CG BstXI 191 CCATCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 757 GCTGAGC GC^T[ACGT]AGC CfoI 80 GCGC GCG^C DdeI 758 CTGAG C^T[ACGT]AG HhaI 80 GCGC GCG^C Hin1I 16 GACGCC G[GA]^CG[CT]C HinP1I 80 GCGC G^CGC KspI 28 CCGCGG CCGC^GG MaeI 871 CTAG C^TAG MaeII 296 ACGT A^CGT MseI 719 TTAA T^TAA MvnI 29 CGCG CG^CG NaeI 547 GCCGGC GCC^GGC NcoI 197 CCATGG C^CATGG NgoMIV 547 GCCGGC G^CCGGC NspI 770 ACATGC [GA]CATG^[CT] PaeR7I 650 CTCGAG C^TCGAG PstI 220 CTGCAG CTGCA^G RsaI 264 GTAC GT^AC SacII 28 CCGCGG CCGC^GG SfcI 220 CTGCAG C^T[GA][CT]AG SmlI 650 CTCGAG C^T[CT][GA]AG Tru9I 719 TTAA T^TAA XhoI 650 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Asp700I 270 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 577 GAACCAATTC BmyI 467 GTGCCC G[AGT]GC[ACT]^C 678 GGGCAC BsmAI 302 GAGAC GTCTC(1/5) 934 GAGAC Bsp1286I 467 GTGCCC G[AGT]GC[ACT]^C 678 GGGCAC BsrFI 131 ACCGGC [GA]^CCGG[CT] 547 GCCGGC Cfr10I 131 ACCGGC [GA]^CCGG[CT] 547 GCCGGC DsaI 28 CCGCGG C^C[GA][CT]GG 197 CCATGG EaeI 444 TGGCCG [CT]^GGCC[GA] 833 CGGCCA EcoNI 363 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 804 CCTCATCGAGG EcoT14I 109 CCTTGG C^C[AT][AT]GG 197 CCATGG HgaI 16 GACGC GACGC(5/10) 325 GACGC HinfI 625 GAATC G^A[ACGT]TC 692 GAATC MslI 192 CATCTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 633 CACCTTTGTG StyI 109 CCTTGG C^C[AT][AT]GG 197 CCATGG TfiI 625 GAATC G^A[AT]TC 692 GAATC Tsp509I 582 AATT ^AATT 878 AATT XcmI 632 CCACCTTTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 656 CCAACAAAGCCCTGG XmnI 270 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 577 GAACCAATTC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BcnI BsaJI BseRI BsiYI BslI BsmFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HpaII ItaI MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PvuII Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I XhoII