Restriction map for
pFN21AE5417
(Circular) (Six-base) MAPSORT of: pFN21AE5417.seq Check: 5918 from: 1 to: 5692
pFN21AE5417 5692 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2131 2131
Size: 5692
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3502 278
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3035 1937
Size: 1098 4594
Acc65I G'GTAC_C
Cuts at: 3013 3013
Size: 5692
AclI AA'CG_TT
Cuts at: 1563 3819 4192 1563
Size: 2256 373 3063
Fragments arranged by size:
3063 2256 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2891 3755 5139 5407 1632
Size: 1259 864 1384 268 1917
Fragments arranged by size:
1917 1384 1259 864 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5495
AflIII A'CryG_T
Cuts at: 1203 4591 5568 1203
Size: 3388 977 1327
Fragments arranged by size:
3388 1327 977
AgeI A'CCGG_T
Cuts at: 4523 4523
Size: 5692
AhdI GACnn_n'nnGTC
Cuts at: 4421 4421
Size: 5692
AleI CACnn'nnGTG
Cuts at: 1166 2642 1166
Size: 1476 4216
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5692
AlwNI CAG_nnn'CTG
Cuts at: 2192 3435 5007 2192
Size: 1243 1572 2877
Fragments arranged by size:
2877 1572 1243
ApaLI G'TGCA_C
Cuts at: 3749 4905 3749
Size: 1156 4536
ApoI r'AATT_y
Cuts at: 1493 3001 3227 3379 5488 1493
Size: 1508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4246 160
Size: 872 3214 1606
Fragments arranged by size:
3214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
AvaI C'yCGr_G
Cuts at: 1870 1943 2655 3017 1870
Size: 73 712 362 4545
Fragments arranged by size:
4545 712 362 73
BamHI G'GATC_C
Cuts at: 3022 3389 3022
Size: 367 5325
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2684 3013 4468 618
Size: 325 217 627 897 329 1455 1842
Fragments arranged by size:
1842 1455 897 627 329 325 217
BanII G_rGCy'C
Cuts at: 1391 3011 1391
Size: 1620 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2251 928
Size: 971 352 4369
Fragments arranged by size:
4369 971 352
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3883 3917 1903
Size: 34 1946 34 3678
Fragments arranged by size:
3678 1946 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3584 4794 3584
Size: 1210 4482
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5692
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2598 4303 136
Size: 107 122 71 631 605 926 1705 1525
Fragments arranged by size:
1705 1525 926 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5687 5687
Size: 5692
BlpI GC'TnA_GC
Cuts at: 2763 3109 2763
Size: 346 5346
Bme1580I G_kGCm'C
Cuts at: 1790 2476 2687 3753 4909 1790
Size: 686 211 1066 1156 2573
Fragments arranged by size:
2573 1156 1066 686 211
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5692
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3423 4381 442
Size: 416 53 83 399 24 2006 958 1753
Fragments arranged by size:
2006 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5692
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2390 4352 1239
Size: 1151 1962 2579
Fragments arranged by size:
2579 1962 1151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3817 4682 4980 3817
Size: 865 298 4529
Fragments arranged by size:
4529 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4355 882
Size: 617 2856 2219
Fragments arranged by size:
2856 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3483 493
Size: 711 2279 2702
Fragments arranged by size:
2702 2279 711
BsaBI GATnn'nnATC
Cuts at: 5686 5686
Size: 5692
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2022 3499 3881 4516
Size: 53 83 186 936 489 1477 382 635
Cuts at: 4516 275
Size: 1451
Fragments arranged by size:
1477 1451 936 635 489 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4125 4523 4797 4944 5355 4125
Size: 398 274 147 411 4462
Fragments arranged by size:
4462 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2391 2421 1178
Size: 30 165 30 988 30 4449
Fragments arranged by size:
4449 988 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 2258 2336 2798 2831 1186
Size: 1015 57 78 462 33 4047
Fragments arranged by size:
4047 1015 462 78 57 33
BseYI C'CCAG_C
Cuts at: 2042 2071 2231 2614 4895 5391 2042
Size: 29 160 383 2281 496 2343
Fragments arranged by size:
2343 2281 496 383 160 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2165 1163
Size: 573 429 4690
Fragments arranged by size:
4690 573 429
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2542 3142 3903 4931 1540
Size: 275 186 541 600 761 1028 2301
Fragments arranged by size:
2301 1028 761 600 541 275 186
BsiHKAI G_wGCw'C
Cuts at: 3753 3838 4909 3753
Size: 85 1071 4536
Fragments arranged by size:
4536 1071 85
BsmI GAATG_Cn'
Cuts at: 3203 3296 3203
Size: 93 5599
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2476 2687 3011 3753 3838 4909 1391
Size: 399 686 211 324 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 686 399 324 211 85
BspEI T'CCGG_A
Cuts at: 5355 5355
Size: 5692
BspHI T'CATG_A
Cuts at: 3581 5311 3581
Size: 1730 3962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2131 3047 844
Size: 1287 916 3489
Fragments arranged by size:
3489 1287 916
BsrBI CCG'CTC
Cuts at: 1394 1823 2163 3579 1394
Size: 429 340 1416 3507
Fragments arranged by size:
3507 1416 429 340
BsrDI GCAATG_nn'
Cuts at: 65 1248 4187 4361 65
Size: 1183 2939 174 1396
Fragments arranged by size:
2939 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2136 2552 4336 4523 1700
Size: 110 326 416 1784 187 2869
Fragments arranged by size:
2869 1784 416 326 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5692
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5692
BssSI C'ACGA_G
Cuts at: 3752 4764 3752
Size: 1012 4680
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2776 794
Size: 1982 3710
BstBI TT'CG_AA
Cuts at: 4496 4496
Size: 5692
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5692
BstXI CCAn_nnnn'nTGG
Cuts at: 2203 2203
Size: 5692
BstYI r'GATC_y
Cuts at: 1972 2188 2206 2247 2651 2866 3022 3389 3774
Size: 216 18 41 404 215 156 367 385
Cuts at: 3774 3791 5243 5329 5341 5687 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 404 385 367 346 216 215
156 86 41 18 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2033 2202 513
Size: 552 939 29 169 4003
Fragments arranged by size:
4003 939 552 169 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4953
Fragments arranged by size:
4953 683 56
BtsI GCAGTG_nn'
Cuts at: 3208 4002 4022 3208
Size: 794 20 4878
Fragments arranged by size:
4878 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5692
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5657
DraI TTT'AAA
Cuts at: 2996 2996
Size: 5692
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4699 809
Size: 507 3383 1802
Fragments arranged by size:
3383 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2449 2838 3139
Size: 54 1425 50 275 18 619 389 301
Cuts at: 3139 4028 5657 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 619 389 301 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3139 1537
Size: 275 1327 4090
Fragments arranged by size:
4090 1327 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3622 1353
Size: 2269 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4279 4653 4799 387
Size: 1064 138 2690 374 146 1280
Fragments arranged by size:
2690 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2390 2891 3755 4352 5139 5407 1239
Size: 393 758 501 864 597 787 268 1524
Fragments arranged by size:
1524 864 787 758 597 501 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2372 2813 2372
Size: 441 5251
EcoO109I rG'GnC_Cy
Cuts at: 2039 2600 2611 2830 2039
Size: 561 11 219 4901
Fragments arranged by size:
4901 561 219 11
EcoRI G'AATT_C
Cuts at: 3001 3379 3001
Size: 378 5314
FspI TGC'GCA
Cuts at: 4198 4198
Size: 5692
HaeII r_GCGC'y
Cuts at: 1678 4839 1678
Size: 3161 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2682 2714 4333 4365 4407
Size: 32 135 32 1110 32 1619 32 42
Cuts at: 4407 4439 1373
Size: 32 2626
Fragments arranged by size:
2626 1619 1110 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3036 3282 669
Size: 988 281 1098 246 3079
Fragments arranged by size:
3079 1098 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5692
HpaI GTT'AAC
Cuts at: 3282 3282
Size: 5692
KpnI G_GTAC'C
Cuts at: 3017 3017
Size: 5692
MfeI C'AATT_G
Cuts at: 3291 3291
Size: 5692
MluI A'CGCG_T
Cuts at: 5568 5568
Size: 5692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2339 4806 4990 1305
Size: 1034 2467 184 2007
Fragments arranged by size:
2467 2007 1034 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3870
Fragments arranged by size:
3870 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2201 2642 3650 4009 4168 518
Size: 648 1035 441 1008 359 159 2042
Fragments arranged by size:
2042 1035 1008 648 441 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2046 2090 2098 2149 2341 2735 3108
Size: 429 11 44 8 51 192 394 373
Cuts at: 3108 3785 4933 5178 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 429 394 373 245 192
51 44 11 8
NaeI GCC'GGC
Cuts at: 2554 2554
Size: 5692
NcoI C'CATG_G
Cuts at: 513 1065 2004 2202 513
Size: 552 939 198 4003
Fragments arranged by size:
4003 939 552 198
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5692
NgoMIV G'CCGG_C
Cuts at: 2552 2552
Size: 5692
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5692
NotI GC'GGCC_GC
Cuts at: 3139 3139
Size: 5692
NspI r_CATG'y
Cuts at: 1132 1229 2779 3050 4595 1132
Size: 97 1550 271 1545 2229
Fragments arranged by size:
2229 1550 1545 271 97
PasI CC'CwG_GG
Cuts at: 1348 2671 1348
Size: 1323 4369
PciI A'CATG_T
Cuts at: 4591 4591
Size: 5692
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5692
PfoI T'CCnGG_A
Cuts at: 2346 2434 2518 2826 2906 2346
Size: 88 84 308 80 5132
Fragments arranged by size:
5132 308 88 84 80
PmeI GTTT'AAAC
Cuts at: 2996 2996
Size: 5692
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3761 3793 5274 5306 3761
Size: 32 1481 32 4147
Fragments arranged by size:
4147 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2039 2611 2830 2039
Size: 572 219 4901
Fragments arranged by size:
4901 572 219
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5692
PsiI TTA'TAA
Cuts at: 3262 3262
Size: 5692
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2261 2293 2261
Size: 32 5660
PstI C_TGCA'G
Cuts at: 830 2229 3044 830
Size: 1399 815 3478
Fragments arranged by size:
3478 1399 815
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5692
PvuII CAG'CTG
Cuts at: 2098 2341 2735 2098
Size: 243 394 5055
Fragments arranged by size:
5055 394 243
SacII CC_GC'GG
Cuts at: 2036 2036
Size: 5692
SalI G'TCGA_C
Cuts at: 1936 3034 1936
Size: 1098 4594
SanDI GG'GwC_CC
Cuts at: 2039 2611 2039
Size: 572 5120
SbfI CC_TGCA'GG
Cuts at: 3044 3044
Size: 5692
ScaI AGT'ACT
Cuts at: 3940 5652 3940
Size: 1712 3980
SfcI C'TryA_G
Cuts at: 826 1045 2225 3040 4175 4856 5047 826
Size: 219 1180 815 1135 681 191 1471
Fragments arranged by size:
1471 1180 1135 815 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5692
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5692
SmaI CCC'GGG
Cuts at: 1872 3019 1872
Size: 1147 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2655 3796 4697 4959 820
Size: 197 926 712 1141 901 262 1553
Fragments arranged by size:
1553 1141 926 901 712 262 197
SnaBI TAC'GTA
Cuts at: 493 3483 493
Size: 2990 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5692
SphI G_CATG'C
Cuts at: 1132 3050 1132
Size: 1918 3774
SspI AAT'ATT
Cuts at: 5 52 3616 5
Size: 47 3564 2081
Fragments arranged by size:
3564 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5692
StyI C'CwwG_G
Cuts at: 513 1065 2004 2114 2202 3131 513
Size: 552 939 110 88 929 3074
Fragments arranged by size:
3074 939 929 552 110 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2727 3719 3736 3889 4074 4519 325
Size: 1255 1147 992 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1147 992 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2268 3938 5650
Size: 274 80 33 51 1443 291 1670 1712
Cuts at: 5650 96
Size: 138
Fragments arranged by size:
1712 1670 1443 291 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5652 5684 5652
Size: 32 5660
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5692
XbaI T'CTAG_A
Cuts at: 3028 3028
Size: 5692
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2644 2668 2644
Size: 24 5668
XhoI C'TCGA_G
Cuts at: 1943 2655 1943
Size: 712 4980
XmaI C'CCGG_G
Cuts at: 1870 3017 1870
Size: 1147 4545
XmnI GAAnn'nnTTC
Cuts at: 2279 2586 3821 2279
Size: 307 1235 4150
Fragments arranged by size:
4150 1235 307
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3500 276
Size: 53 83 186 936 1966 2468
Fragments arranged by size:
2468 1966 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacI SapI
SexAI SfiI SfoI SrfI SwaI