################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AvrII BbeI BbrPI BbsI BbvCI BcgI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiWI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FokI FseI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MaeIII MamI MfeI MluI MroI MspI MunI MvnI MwoI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tsp45I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AciI 563 GCGG CCGC(-3/-1) Alw44I 539 GTGCAC G^TGCAC ApaLI 539 GTGCAC G^TGCAC AviII 720 TGCGCA TGC^GCA BalI 1221 TGGCCA TGG^CCA BanI 1167 GGCACC G^G[CT][GA]CC BanII 775 GAGCTC G[GA]GC[CT]^C BbvI 55 GCAGC GCAGC(8/12) BclI 222 TGATCA T^GATCA BsaI 341 GAGACC GGTCTC(1/5) BsaJI 1189 CCATGG C^C[ACGT][ACGT]GG BseRI 34 CTCCTC GAGGAG(10/8) BsiEI 1365 CGACCG CG[GA][CT]^CG BsmBI 531 CGTCTC CGTCTC(1/5) BsmI 261 GAATGC GAATGC(1/-1) BssKI 1164 CCAGG ^CC[ACGT]GG BstNI 1164 CCAGG CC^[AT]GG CfoI 721 GCGC GCG^C Cfr13I 132 GGGCC G^G[ACGT]CC DsaI 1189 CCATGG C^C[GA][CT]GG EcoRII 1164 CCAGG ^CC[AT]GG EcoT14I 1189 CCATGG C^C[AT][AT]GG FbaI 222 TGATCA T^GATCA Fnu4HI 55 GCAGC GC^[ACGT]GC FspI 720 TGCGCA TGC^GCA HhaI 721 GCGC GCG^C HinP1I 721 GCGC G^CGC HincII 308 GTCAAC GT[CT]^[GA]AC HindII 308 GTCAAC GT[CT]^[GA]AC HphI 1198 GGTGA GGTGA(8/7) ItaI 55 GCAGC GC^[ACGT]GC MaeII 621 ACGT A^CGT MluNI 1221 TGGCCA TGG^CCA MscI 1221 TGGCCA TGG^CCA MseI 1358 TTAA T^TAA MspA1I 56 CAGCTG C[AC]G^C[GT]G MvaI 1164 CCAGG CC^[AT]GG NcoI 1189 CCATGG C^CATGG PvuII 56 CAGCTG CAG^CTG SacI 775 GAGCTC GAGCT^C SapI 595 GAAGAGC GCTCTTC(1/4) Sau96I 132 GGGCC G^G[ACGT]CC ScrFI 1164 CCAGG CC^[ACGT]GG SfaNI 214 GATGC GCATC(5/9) SmlI 428 CTCAAG C^T[CT][GA]AG SspI 473 AATATT AAT^ATT StyI 1189 CCATGG C^C[AT][AT]GG Tru9I 1358 TTAA T^TAA TseI 55 GCAGC G^C[AT]GC TspRI 1332 CAGTG CA[GC]TG[ACGT][ACGT]^ XcmI 135 CCAGCACTATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 103 GAATTT [GA]^AATT[CT] 633 AAATTC ApoI 103 GAATTT [GA]^AATT[CT] 633 AAATTC AspHI 539 GTGCAC G[AT]GC[AT]^C 775 GAGCTC BamHI 565 GGATCC G^GATCC 745 GGATCC BciVI 925 GTATCC GTATCC(6/5) 1105 GTATCC BmyI 539 GTGCAC G[AGT]GC[ACT]^C 775 GAGCTC BsgI 913 GTGCAG GTGCAG(16/14) 1093 GTGCAG BsiHKAI 539 GTGCAC G[AT]GC[AT]^C 775 GAGCTC Bsp1286I 539 GTGCAC G[AGT]GC[ACT]^C 775 GAGCTC BspHI 225 TCATGA T^CATGA 1257 TCATGA EaeI 837 TGGCCG [CT]^GGCC[GA] 1221 TGGCCA EarI 595 GAAGAG CTCTTC(1/4) 955 GAAGAG Ksp632I 595 GAAGAG CTCTTC(1/4) 955 GAAGAG PflMI 660 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1189 CCATGGCATGG RcaI 225 TCATGA T^CATGA 1257 TCATGA TfiI 172 GAATC G^A[AT]TC 795 GAATC Van91I 660 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1189 CCATGGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AfaI AluI AlwI BfaI BsiYI BslI BsmAI BsmFI BsrI BstYI Cac8I DdeI DpnII HaeIII HinfI MaeI MboI MboII MflI MnlI MslI NdeII NlaIII NlaIV PleI RsaI Sau3AI Tsp509I XhoII