Restriction map for
pFN21AB7040
(Circular) (Six-base) MAPSORT of: pFN21AB7040.seq Check: 6638 from: 1 to: 6091
pFN21AB7040 6091 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3901 278
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3434 1937
Size: 1497 4594
Acc65I G'GTAC_C
Cuts at: 3412 3412
Size: 6091
AclI AA'CG_TT
Cuts at: 1563 4218 4591 1563
Size: 2655 373 3063
Fragments arranged by size:
3063 2655 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2619 2979 4154 5538 5806 1632
Size: 987 360 1175 1384 268 1917
Fragments arranged by size:
1917 1384 1175 987 360 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5894
AflIII A'CryG_T
Cuts at: 1203 4990 5967 1203
Size: 3787 977 1327
Fragments arranged by size:
3787 1327 977
AgeI A'CCGG_T
Cuts at: 4922 4922
Size: 6091
AhdI GACnn_n'nnGTC
Cuts at: 4820 4820
Size: 6091
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6091
AlwNI CAG_nnn'CTG
Cuts at: 3834 5406 3834
Size: 1572 4519
ApaLI G'TGCA_C
Cuts at: 2544 4148 5304 2544
Size: 1604 1156 3331
Fragments arranged by size:
3331 1604 1156
ApoI r'AATT_y
Cuts at: 1493 2108 2638 3400 3626 3778 5887 1493
Size: 615 530 762 226 152 2109 1697
Fragments arranged by size:
2109 1697 762 615 530 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4645 160
Size: 872 3613 1606
Fragments arranged by size:
3613 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
AvaI C'yCGr_G
Cuts at: 1870 1943 3416 1870
Size: 73 1473 4545
Fragments arranged by size:
4545 1473 73
BamHI G'GATC_C
Cuts at: 2570 2750 3421 3788 2570
Size: 180 671 367 4873
Fragments arranged by size:
4873 671 367 180
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3172 3412 4867 618
Size: 325 217 627 1385 240 1455 1842
Fragments arranged by size:
1842 1455 1385 627 325 240 217
BanII G_rGCy'C
Cuts at: 1391 2784 3410 1391
Size: 1393 626 4072
Fragments arranged by size:
4072 1393 626
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4282 4316 1903
Size: 34 2345 34 3678
Fragments arranged by size:
3678 2345 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2941 3121 3983 5193 2941
Size: 180 862 1210 3839
Fragments arranged by size:
3839 1210 862 180
BclI T'GATC_A
Cuts at: 1548 2227 1548
Size: 679 5412
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4702 136
Size: 107 122 71 631 605 3030 1525
Fragments arranged by size:
3030 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6086 6086
Size: 6091
BlpI GC'TnA_GC
Cuts at: 3508 3508
Size: 6091
Bme1580I G_kGCm'C
Cuts at: 1790 2548 4152 5308 1790
Size: 758 1604 1156 2573
Fragments arranged by size:
2573 1604 1156 758
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6091
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2997 3822 4780
Size: 416 53 83 399 24 1580 825 958
Cuts at: 4780 442
Size: 1753
Fragments arranged by size:
1753 1580 958 825 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4751 1239
Size: 3512 2579
Bpu10I CC'TnA_GC
Cuts at: 3183 3183
Size: 6091
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2418 4216 5081 5379 2418
Size: 1798 865 298 3130
Fragments arranged by size:
3130 1798 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2340 4754 882
Size: 617 841 2414 2219
Fragments arranged by size:
2414 2219 841 617
BsaAI yAC'GTr
Cuts at: 493 1204 3882 493
Size: 711 2678 2702
Fragments arranged by size:
2702 2678 711
BsaBI GATnn'nnATC
Cuts at: 6085 6085
Size: 6091
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3898 4280 4915 275
Size: 53 83 186 936 2365 382 635 1451
Fragments arranged by size:
2365 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4524 4922 5196 5343 5754 4524
Size: 398 274 147 411 4861
Fragments arranged by size:
4861 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2307 2337 1178
Size: 30 165 30 904 30 4932
Fragments arranged by size:
4932 904 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2030 1186
Size: 844 5247
BseYI C'CCAG_C
Cuts at: 5294 5790 5294
Size: 496 5595
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2939 3119 1163
Size: 573 1203 180 4135
Fragments arranged by size:
4135 1203 573 180
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 3541 4302 5330 1540
Size: 275 186 1372 168 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186 168
BsiHKAI G_wGCw'C
Cuts at: 2548 2784 4152 4237 5308 2548
Size: 236 1368 85 1071 3331
Fragments arranged by size:
3331 1368 1071 236 85
BsmI GAATG_Cn'
Cuts at: 2272 3602 3695 2272
Size: 1330 93 4668
Fragments arranged by size:
4668 1330 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2542 2542
Size: 6091
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2548 2784 3410 4152 4237 5308 1391
Size: 399 758 236 626 742 85 1071 2174
Fragments arranged by size:
2174 1071 758 742 626 399 236 85
BspEI T'CCGG_A
Cuts at: 5754 5754
Size: 6091
BspHI T'CATG_A
Cuts at: 2230 3262 3980 5710 2230
Size: 1032 718 1730 2611
Fragments arranged by size:
2611 1730 1032 718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3446 844
Size: 2602 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3978 1394
Size: 429 2155 3507
Fragments arranged by size:
3507 2155 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4586 4760 65
Size: 1183 3338 174 1396
Fragments arranged by size:
3338 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4735 4922 1700
Size: 110 2925 187 2869
Fragments arranged by size:
2925 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6091
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6091
BssSI C'ACGA_G
Cuts at: 4151 5163 4151
Size: 1012 5079
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6091
BstBI TT'CG_AA
Cuts at: 4895 4895
Size: 6091
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6091
BstYI r'GATC_y
Cuts at: 1972 2570 2750 3290 3421 3788 4173 4190 5642
Size: 598 180 540 131 367 385 17 1452
Cuts at: 5642 5728 5740 6086 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 598 540 385 367 346 180
131 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3194 513
Size: 552 2129 3410
Fragments arranged by size:
3410 2129 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5352
Fragments arranged by size:
5352 683 56
BtsI GCAGTG_nn'
Cuts at: 3607 4401 4421 3607
Size: 794 20 5277
Fragments arranged by size:
5277 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6091
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6056
DraI TTT'AAA
Cuts at: 3395 3395
Size: 6091
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5098 809
Size: 507 3782 1802
Fragments arranged by size:
3782 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2842 3226 3538
Size: 54 1425 50 275 18 1012 384 312
Cuts at: 3538 4427 6056 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1012 889 384 312 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3538 1537
Size: 275 1726 4090
Fragments arranged by size:
4090 1726 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2595 2955 4021 1353
Size: 1242 360 1066 3423
Fragments arranged by size:
3423 1242 1066 360
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2583 4678 5052 5198 387
Size: 1064 138 994 2095 374 146 1280
Fragments arranged by size:
2095 1280 1064 994 374 146 138
EcoICRI GAG'CTC
Cuts at: 2782 2782
Size: 6091
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2619 2979 4154 4751 5538 5806 1239
Size: 393 987 360 1175 597 787 268 1524
Fragments arranged by size:
1524 1175 987 787 597 393 360 268
EcoRI G'AATT_C
Cuts at: 3400 3778 3400
Size: 378 5713
FspI TGC'GCA
Cuts at: 2727 4597 2727
Size: 1870 4221
HaeII r_GCGC'y
Cuts at: 1678 5238 1678
Size: 3560 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2410 2442 2803 2835 4732
Size: 32 135 32 838 32 361 32 1897
Cuts at: 4732 4764 4806 4838 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1897 838 361 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2315 3435 3681 669
Size: 988 281 377 1120 246 3079
Fragments arranged by size:
3079 1120 988 377 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6091
HpaI GTT'AAC
Cuts at: 3681 3681
Size: 6091
KpnI G_GTAC'C
Cuts at: 3416 3416
Size: 6091
MfeI C'AATT_G
Cuts at: 3690 3690
Size: 6091
MluI A'CGCG_T
Cuts at: 5967 5967
Size: 6091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2767 5205 5389 1305
Size: 1462 2438 184 2007
Fragments arranged by size:
2438 2007 1462 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3228 10
Size: 54 1425 343 1396 2873
Fragments arranged by size:
2873 1425 1396 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2217 2304 3285 4049 4408 4567 518
Size: 648 1051 87 981 764 359 159 2042
Fragments arranged by size:
2042 1051 981 764 648 359 159 87
MspA1I CmG'CkG
Cuts at: 1606 2063 3507 4184 5332 5577 1606
Size: 457 1444 677 1148 245 2120
Fragments arranged by size:
2120 1444 1148 677 457 245
NcoI C'CATG_G
Cuts at: 513 1065 3194 513
Size: 552 2129 3410
Fragments arranged by size:
3410 2129 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6091
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6091
NotI GC'GGCC_GC
Cuts at: 3538 3538
Size: 6091
NspI r_CATG'y
Cuts at: 1132 1229 3449 4994 1132
Size: 97 2220 1545 2229
Fragments arranged by size:
2229 2220 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6091
PciI A'CATG_T
Cuts at: 4990 4990
Size: 6091
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2671 3200 1108
Size: 1563 529 3999
Fragments arranged by size:
3999 1563 529
PmeI GTTT'AAAC
Cuts at: 3395 3395
Size: 6091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4160 4192 5673 5705 4160
Size: 32 1481 32 4546
Fragments arranged by size:
4546 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6091
PsiI TTA'TAA
Cuts at: 3661 3661
Size: 6091
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3237 3269 3237
Size: 32 6059
PstI C_TGCA'G
Cuts at: 830 3443 830
Size: 2613 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6091
PvuII CAG'CTG
Cuts at: 2063 2063
Size: 6091
SacI G_AGCT'C
Cuts at: 2784 2784
Size: 6091
SalI G'TCGA_C
Cuts at: 1936 3433 1936
Size: 1497 4594
SapI GCTCTTCn'nnn_
Cuts at: 2595 2595
Size: 6091
SbfI CC_TGCA'GG
Cuts at: 3443 3443
Size: 6091
ScaI AGT'ACT
Cuts at: 4339 6051 4339
Size: 1712 4379
SfcI C'TryA_G
Cuts at: 826 1045 3439 4574 5255 5446 826
Size: 219 2394 1135 681 191 1471
Fragments arranged by size:
2394 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6091
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6091
SmaI CCC'GGG
Cuts at: 1872 3418 1872
Size: 1546 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2433 4195 5096 5358 820
Size: 197 926 490 1762 901 262 1553
Fragments arranged by size:
1762 1553 926 901 490 262 197
SnaBI TAC'GTA
Cuts at: 493 3882 493
Size: 3389 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6091
SphI G_CATG'C
Cuts at: 1132 3449 1132
Size: 2317 3774
SspI AAT'ATT
Cuts at: 5 52 2480 4015 5
Size: 47 2428 1535 2081
Fragments arranged by size:
2428 2081 1535 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6091
StyI C'CwwG_G
Cuts at: 513 1065 3194 3530 513
Size: 552 2129 336 3074
Fragments arranged by size:
3074 2129 552 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3387 4118 4135 4288 4473 4918 325
Size: 1255 1807 731 17 153 185 445 1498
Fragments arranged by size:
1807 1498 1255 731 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2026 2704 2879
Size: 274 80 33 51 1443 49 678 175
Cuts at: 2879 4337 6049 96
Size: 1458 1712 138
Fragments arranged by size:
1712 1458 1443 678 274 175 138 80
51 49 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6051 6083 6051
Size: 32 6059
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6091
XbaI T'CTAG_A
Cuts at: 3427 3427
Size: 6091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2147 2147
Size: 6091
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6091
XmaI C'CCGG_G
Cuts at: 1870 3416 1870
Size: 1546 4545
XmnI GAAnn'nnTTC
Cuts at: 4220 4220
Size: 6091
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3899 276
Size: 53 83 186 936 2365 2468
Fragments arranged by size:
2468 2365 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI