(Linear) (Six-base) MAPSORT of: KIBB7040.seq  Check: 9288  from: 1  to: 1386



KIBB7040.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614     974    1386 
   Size:        614     360     412

  Fragments arranged by size: 

                 614     412     360
 
ApaLI G'TGCA_C

Cuts at:      0     539    1386 
   Size:        539     847
 
ApoI r'AATT_y

Cuts at:      0     103     633    1386 
   Size:        103     530     753

  Fragments arranged by size: 

                 753     530     103
 
BamHI G'GATC_C

Cuts at:      0     565     745    1386 
   Size:        565     180     641

  Fragments arranged by size: 

                 641     565     180
 
BanI G'GyrC_C

Cuts at:      0    1167    1386 
   Size:       1167     219
 
BanII G_rGCy'C

Cuts at:      0     779    1386 
   Size:        779     607
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     936    1116    1386 
   Size:        936     180     270

  Fragments arranged by size: 

                 936     270     180
 
BclI T'GATC_A

Cuts at:      0     222    1386 
   Size:        222    1164
 
Bme1580I G_kGCm'C

Cuts at:      0     543    1386 
   Size:        543     843
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     992    1386 
   Size:        992     394
 
Bpu10I CC'TnA_GC

Cuts at:      0    1178    1386 
   Size:       1178     208
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413    1386 
   Size:        413     973
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     335    1386 
   Size:        335    1051
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     302     332    1386 
   Size:        302      30    1054

  Fragments arranged by size: 

                1054     302      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25    1386 
   Size:         25    1361
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     934    1114    1386 
   Size:        934     180     272

  Fragments arranged by size: 

                 934     272     180
 
BsiEI CG_ry'CG

Cuts at:      0    1368    1386 
   Size:       1368      18
 
BsiHKAI G_wGCw'C

Cuts at:      0     543     779    1386 
   Size:        543     236     607

  Fragments arranged by size: 

                 607     543     236
 
BsmI GAATG_Cn'

Cuts at:      0     267    1386 
   Size:        267    1119
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     537    1386 
   Size:        537     849
 
Bsp1286I G_dGCh'C

Cuts at:      0     543     779    1386 
   Size:        543     236     607

  Fragments arranged by size: 

                 607     543     236
 
BspHI T'CATG_A

Cuts at:      0     225    1257    1386 
   Size:        225    1032     129

  Fragments arranged by size: 

                1032     225     129
 
BstYI r'GATC_y

Cuts at:      0     565     745    1285    1386 
   Size:        565     180     540     101

  Fragments arranged by size: 

                 565     540     180     101
 
BtgI C'CryG_G

Cuts at:      0    1189    1386 
   Size:       1189     197
 
EaeI y'GGCC_r

Cuts at:      0     837    1221    1386 
   Size:        837     384     165

  Fragments arranged by size: 

                 837     384     165
 
EarI CTCTTCn'nnn_

Cuts at:      0     590     950    1386 
   Size:        590     360     436

  Fragments arranged by size: 

                 590     436     360
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     578    1386 
   Size:        578     808
 
EcoICRI GAG'CTC

Cuts at:      0     777    1386 
   Size:        777     609
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     614     974    1386 
   Size:        614     360     412

  Fragments arranged by size: 

                 614     412     360
 
FspI TGC'GCA

Cuts at:      0     722    1386 
   Size:        722     664
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     405     437     798     830    1386 
   Size:        405      32     361      32     556

  Fragments arranged by size: 

                 556     405     361      32      32
 
HincII GTy'rAC

Cuts at:      0     310    1386 
   Size:        310    1076
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     762    1386 
   Size:        762     624
 
MscI TGG'CCA

Cuts at:      0    1223    1386 
   Size:       1223     163
 
MslI CAynn'nnrTG

Cuts at:      0     212     299    1280    1386 
   Size:        212      87     981     106

  Fragments arranged by size: 

                 981     212     106      87
 
MspA1I CmG'CkG

Cuts at:      0      58    1386 
   Size:         58    1328
 
NcoI C'CATG_G

Cuts at:      0    1189    1386 
   Size:       1189     197
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     666    1195    1386 
   Size:        666     529     191

  Fragments arranged by size: 

                 666     529     191
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1232    1264    1386 
   Size:       1232      32     122

  Fragments arranged by size: 

                1232     122      32
 
PvuII CAG'CTG

Cuts at:      0      58    1386 
   Size:         58    1328
 
SacI G_AGCT'C

Cuts at:      0     779    1386 
   Size:        779     607
 
SapI GCTCTTCn'nnn_

Cuts at:      0     590    1386 
   Size:        590     796
 
SmlI C'TyrA_G

Cuts at:      0     428    1386 
   Size:        428     958
 
SspI AAT'ATT

Cuts at:      0     475    1386 
   Size:        475     911
 
StyI C'CwwG_G

Cuts at:      0    1189    1386 
   Size:       1189     197
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1382    1386 
   Size:       1382       4
 
TatI w'GTAC_w

Cuts at:      0      21     699     874    1386 
   Size:         21     678     175     512

  Fragments arranged by size: 

                 678     512     175      21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     142    1386 
   Size:        142    1244
 

 Enzymes that do cut:

     AcuI    ApaLI     ApoI    BamHI     BanI    BanII    BciVI     BclI
 Bme1580I     BmrI   Bpu10I    BpuEI     BsaI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BstYI     BtgI
     EaeI     EarI     EciI  EcoICRI  Eco57MI     FspI    Hin4I   HincII
     MmeI     MscI     MslI   MspA1I     NcoI    PflMI     PsrI    PvuII
     SacI     SapI     SmlI     SspI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI     BbsI    BbvCI
     BcgI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
     BpmI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI    BsiWI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PstI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI