################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AlwNI Aor51HI ApaLI AseI AsnI Asp700I Asp718I AspI BbvCI BciVI BclI BfrI BsaBI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eco47III EcoO65I EcoRV EcoT22I FbaI FseI HincII HindII HindIII HinfI HpaI KpnI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tth111I Van91I XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AcsI 301 GAATTC [GA]^AATT[CT] AhdI 925 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1313 CCTCAGG CC^T[ACGT]AGG ApaI 111 GGGCCC GGGCC^C ApoI 301 GAATTC [GA]^AATT[CT] AscI 1065 GGCGCGCC GG^CGCGCC AspEI 925 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 125 GGATCC G^GATCC BanII 111 GGGCCC G[GA]GC[CT]^C BbrPI 370 CACGTG CAC^GTG BbsI 391 GTCTTC GAAGAC(2/6) BcgI 992 GCAAGGAGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 391 GTCTTC GAAGAC(2/6) BsaAI 370 CACGTG [CT]AC^GT[GA] BsaWI 41 TCCGGT [AT]^CCGG[AT] BsmBI 672 CGTCTC CGTCTC(1/5) BsrBI 910 CCGCTC CCGCTC(-3/-3) Bsu36I 1313 CCTCAGG CC^T[ACGT]AGG EagI 752 CGGCCG C^GGCCG Eam1105I 925 GACATTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 661 CTCTTC CTCTTC(1/4) EclXI 752 CGGCCG C^GGCCG Eco52I 752 CGGCCG C^GGCCG Eco81I 1313 CCTCAGG CC^T[ACGT]AGG EcoNI 1008 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 301 GAATTC G^AATTC HphI 1358 TCACC GGTGA(8/7) Ksp632I 661 CTCTTC CTCTTC(1/4) MaeIII 1288 GTGAC ^GT[ACGT]AC MseI 1214 TTAA T^TAA NcoI 626 CCATGG C^CATGG NotI 751 GCGGCCGC GC^GGCCGC PaeR7I 823 CTCGAG C^TCGAG PmaCI 370 CACGTG CAC^GTG PmlI 370 CACGTG CAC^GTG SapI 660 GCTCTTC GCTCTTC(1/4) SbfI 234 CCTGCAGG CCTGCA^GG ScaI 827 AGTACT AGT^ACT Sse8387I 234 CCTGCAGG CCTGCA^GG Tru9I 1214 TTAA T^TAA Tsp45I 1288 GTGAC ^GT[GC]AC Tsp509I 302 AATT ^AATT XbaI 1533 TCTAGA T^CTAGA XcmI 1225 CCAGCCTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 823 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwI 126 GATCC GGATC(4/5) 125 GGATC AvaII 15 GGTCC G^G[AT]CC 531 GGACC AviII 1040 TGCGCA TGC^GCA 1421 TGCGCA BalI 360 TGGCCA TGG^CCA 402 TGGCCA BglII 548 AGATCT A^GATCT 1530 AGATCT BlpI 186 GCTGAGC GC^T[ACGT]AGC 1455 GCTGAGC Bpu1102I 186 GCTGAGC GC^T[ACGT]AGC 1455 GCTGAGC BsiEI 312 CGACCG CG[GA][CT]^CG 752 CGGCCG BsmAI 1492 GAGAC GTCTC(1/5) 673 GTCTC BsmFI 500 GGGAC GGGAC(10/14) 1518 GGGAC BspMI 365 ACCTGC ACCTGC(4/8) 1023 GCAGGT BsrFI 314 ACCGGC [GA]^CCGG[CT] 1391 ACCGGC BstXI 677 CCATCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1225 CCAGCCTGCTGG CelII 186 GCTGAGC GC^T[ACGT]AGC 1455 GCTGAGC Cfr10I 314 ACCGGC [GA]^CCGG[CT] 1391 ACCGGC FspI 1040 TGCGCA TGC^GCA 1421 TGCGCA KspI 242 CCGCGG CCGC^GG 773 CCGCGG MluNI 360 TGGCCA TGG^CCA 402 TGGCCA MscI 360 TGGCCA TGG^CCA 402 TGGCCA MslI 678 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1335 CATCCTGGTG PstI 235 CTGCAG CTGCA^G 1526 CTGCAG SacII 242 CCGCGG CCGC^GG 773 CCGCGG SfcI 235 CTGCAG C^T[GA][CT]AG 1526 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaI AvrII BanI BbeI BbvI BcnI BfaI BglI BlnI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsoBI Bsp1286I BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI MaeI MaeII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfoI SmaI SmlI StyI TaqI TseI TspRI TthHB8I XhoII XmaCI XmaI