(Circular) (Six-base) MAPSORT of: pFN21AE4128.seq Check: 7076 from: 1 to: 6283 pFN21AE4128 6283 bp With 182 enzymes: SgfI * March 28, 2012 13:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2379 3019 2379 Size: 640 5643 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4093 278 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3626 1937 Size: 1689 4594 Acc65I G'GTAC_C Cuts at: 3604 3604 Size: 6283 AclI AA'CG_TT Cuts at: 1563 4410 4783 1563 Size: 2847 373 3063 Fragments arranged by size: 3063 2847 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2653 4346 5730 5998 1632 Size: 1021 1693 1384 268 1917 Fragments arranged by size: 1917 1693 1384 1021 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6086 AflIII A'CryG_T Cuts at: 1203 5182 6159 1203 Size: 3979 977 1327 Fragments arranged by size: 3979 1327 977 AgeI A'CCGG_T Cuts at: 5114 5114 Size: 6283 AhdI GACnn_n'nnGTC Cuts at: 2935 5012 2935 Size: 2077 4206 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6283 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3041 3158 1184 Size: 1857 117 4309 Fragments arranged by size: 4309 1857 117 AlwNI CAG_nnn'CTG Cuts at: 4026 5598 4026 Size: 1572 4711 ApaI G_GGCC'C Cuts at: 2120 2120 Size: 6283 ApaLI G'TGCA_C Cuts at: 4340 5496 4340 Size: 1156 5127 ApoI r'AATT_y Cuts at: 1493 2306 3592 3818 3970 6079 1493 Size: 813 1286 226 152 2109 1697 Fragments arranged by size: 2109 1697 1286 813 226 152 AscI GG'CGCG_CC Cuts at: 3071 3071 Size: 6283 AseI AT'TA_AT Cuts at: 160 1032 4837 160 Size: 872 3805 1606 Fragments arranged by size: 3805 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 AvaI C'yCGr_G Cuts at: 1870 1943 2113 2218 2326 2641 2732 2828 3504 Size: 73 170 105 108 315 91 96 676 Cuts at: 3504 3608 1870 Size: 104 4545 Fragments arranged by size: 4545 676 315 170 108 105 104 96 91 73 AvrII C'CTAG_G Cuts at: 2074 2431 3331 2074 Size: 357 900 5026 Fragments arranged by size: 5026 900 357 BamHI G'GATC_C Cuts at: 2130 3613 3980 2130 Size: 1483 367 4433 Fragments arranged by size: 4433 1483 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2271 2322 2884 3286 3399 Size: 325 217 627 484 51 562 402 113 Cuts at: 3399 3604 5059 618 Size: 205 1455 1842 Fragments arranged by size: 1842 1455 627 562 484 402 325 217 205 113 51 BanII G_rGCy'C Cuts at: 1391 2120 3602 1391 Size: 729 1482 4072 Fragments arranged by size: 4072 1482 729 BbeI G_GCGC'C Cuts at: 2275 2888 3403 2275 Size: 613 515 5155 Fragments arranged by size: 5155 613 515 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2389 928 Size: 971 490 4822 Fragments arranged by size: 4822 971 490 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2986 3020 4474 4508 1903 Size: 34 50 34 965 34 1454 34 3678 Fragments arranged by size: 3678 1454 965 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4175 5385 4175 Size: 1210 5073 BclI T'GATC_A Cuts at: 1548 1548 Size: 6283 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2750 2765 2796 Size: 107 122 71 631 605 1078 15 31 Cuts at: 2796 3015 4894 136 Size: 219 1879 1525 Fragments arranged by size: 1879 1525 1078 631 605 219 122 107 71 31 15 BglII A'GATC_T Cuts at: 2553 3535 6278 2553 Size: 982 2743 2558 Fragments arranged by size: 2743 2558 982 BlpI GC'TnA_GC Cuts at: 2192 3461 3700 2192 Size: 1269 239 4775 Fragments arranged by size: 4775 1269 239 Bme1580I G_kGCm'C Cuts at: 1790 2120 2225 2546 3251 4344 5500 1790 Size: 330 105 321 705 1093 1156 2573 Fragments arranged by size: 2573 1156 1093 705 330 321 105 BmgBI CAC'GTC Cuts at: 1318 2563 3211 1318 Size: 1245 648 4390 Fragments arranged by size: 4390 1245 648 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2434 2883 2990 Size: 416 53 83 399 24 1017 449 107 Cuts at: 2990 3549 4014 4972 442 Size: 559 465 958 1753 Fragments arranged by size: 1753 1017 958 559 465 449 416 399 107 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6283 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3125 3125 Size: 6283 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2495 3434 3515 4943 1239 Size: 1256 939 81 1428 2579 Fragments arranged by size: 2579 1428 1256 939 81 Bpu10I CC'TnA_GC Cuts at: 2339 2339 Size: 6283 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3047 3095 4408 5273 5571 3047 Size: 48 1313 865 298 3759 Fragments arranged by size: 3759 1313 865 298 48 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4946 882 Size: 617 3447 2219 Fragments arranged by size: 3447 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2377 4074 493 Size: 711 1173 1697 2702 Fragments arranged by size: 2702 1697 1173 711 BsaBI GATnn'nnATC Cuts at: 6277 6277 Size: 6283 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2252 2272 2824 2885 Size: 53 83 186 936 719 20 552 61 Cuts at: 2885 3400 4090 4472 5107 275 Size: 515 690 382 635 1451 Fragments arranged by size: 1451 936 719 690 635 552 515 382 186 83 61 53 20 BsaWI w'CCGG_w Cuts at: 2046 4716 5114 5388 5535 5946 2046 Size: 2670 398 274 147 411 2383 Fragments arranged by size: 2670 2383 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2540 2570 3368 3398 3512 Size: 30 165 30 1137 30 798 30 114 Cuts at: 3512 3542 1178 Size: 30 3919 Fragments arranged by size: 3919 1137 798 165 114 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2098 2273 3218 1186 Size: 912 175 945 4251 Fragments arranged by size: 4251 945 912 175 BseYI C'CCAG_C Cuts at: 2230 2490 3097 3229 5486 5982 2230 Size: 260 607 132 2257 496 2531 Fragments arranged by size: 2531 2257 607 496 260 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2333 2357 2585 3047 1163 Size: 573 597 24 228 462 4399 Fragments arranged by size: 4399 597 573 462 228 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2320 2760 3733 4494 5522 1540 Size: 275 186 319 440 973 761 1028 2301 Fragments arranged by size: 2301 1028 973 761 440 319 275 186 BsiHKAI G_wGCw'C Cuts at: 2090 2382 2697 4344 4429 5500 2090 Size: 292 315 1647 85 1071 2873 Fragments arranged by size: 2873 1647 1071 315 292 85 BsmI GAATG_Cn' Cuts at: 3794 3887 3794 Size: 93 6190 BsmBI CGTCTCn'nnnn_ Cuts at: 2683 2683 Size: 6283 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2090 2120 2225 2382 2546 2697 3251 Size: 399 300 30 105 157 164 151 554 Cuts at: 3251 3602 4344 4429 5500 1391 Size: 351 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 554 399 351 300 164 157 151 105 85 30 BspEI T'CCGG_A Cuts at: 5946 5946 Size: 6283 BspHI T'CATG_A Cuts at: 4172 5902 4172 Size: 1730 4553 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2379 3019 3638 844 Size: 1535 640 619 3489 Fragments arranged by size: 3489 1535 640 619 BsrBI CCG'CTC Cuts at: 1394 1823 2917 4170 1394 Size: 429 1094 1253 3507 Fragments arranged by size: 3507 1253 1094 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4778 4952 65 Size: 1183 3530 174 1396 Fragments arranged by size: 3530 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2319 3396 4927 5114 1700 Size: 110 509 1077 1531 187 2869 Fragments arranged by size: 2869 1531 1077 509 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6283 BssHII G'CGCG_C Cuts at: 1925 2196 2198 2200 2210 2300 2463 2465 2579 Size: 271 2 2 10 90 163 2 114 Cuts at: 2579 2594 3071 1925 Size: 15 477 5137 Fragments arranged by size: 5137 477 271 163 114 90 15 10 2 2 2 BssSI C'ACGA_G Cuts at: 4343 5355 4343 Size: 1012 5271 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6283 BstBI TT'CG_AA Cuts at: 5087 5087 Size: 6283 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6283 BstXI CCAn_nnnn'nTGG Cuts at: 2689 3237 2689 Size: 548 5735 BstYI r'GATC_y Cuts at: 1972 2130 2553 3535 3613 3980 4365 4382 5834 Size: 158 423 982 78 367 385 17 1452 Cuts at: 5834 5920 5932 6278 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 982 423 385 367 346 158 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 3319 3319 Size: 6283 BtgI C'CryG_G Cuts at: 513 1065 2247 2631 2763 2778 3267 513 Size: 552 1182 384 132 15 489 3529 Fragments arranged by size: 3529 1182 552 489 384 132 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2689 2827 487 Size: 683 56 1463 138 3943 Fragments arranged by size: 3943 1463 683 138 56 BtsI GCAGTG_nn' Cuts at: 3799 4593 4613 3799 Size: 794 20 5469 Fragments arranged by size: 5469 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6283 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6248 DraI TTT'AAA Cuts at: 3587 3587 Size: 6283 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5290 809 Size: 507 3974 1802 Fragments arranged by size: 3974 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2365 2407 2602 Size: 54 1425 50 275 18 535 42 195 Cuts at: 2602 2757 2775 3007 3730 4619 6248 8 Size: 155 18 232 723 889 1629 43 Fragments arranged by size: 1629 1425 889 723 535 275 232 195 155 54 50 43 42 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2757 3730 1537 Size: 275 945 973 4090 Fragments arranged by size: 4090 973 945 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2672 4213 1353 Size: 1319 1541 3423 Fragments arranged by size: 3423 1541 1319 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3026 4870 5244 5390 387 Size: 1064 138 1437 1844 374 146 1280 Fragments arranged by size: 1844 1437 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2495 2653 3434 3515 4346 4943 5730 Size: 393 863 158 781 81 831 597 787 Cuts at: 5730 5998 1239 Size: 268 1524 Fragments arranged by size: 1524 863 831 787 781 597 393 268 158 81 EcoNI CCTnn'n_nnAGG Cuts at: 3017 3017 Size: 6283 EcoO109I rG'GnC_Cy Cuts at: 2117 2428 3550 2117 Size: 311 1122 4850 Fragments arranged by size: 4850 1122 311 EcoRI G'AATT_C Cuts at: 2306 3592 3970 2306 Size: 1286 378 4619 Fragments arranged by size: 4619 1286 378 FspI TGC'GCA Cuts at: 3047 3428 4789 3047 Size: 381 1361 4541 Fragments arranged by size: 4541 1361 381 FspAI rTGC'GCAy Cuts at: 3047 3047 Size: 6283 HaeII r_GCGC'y Cuts at: 1678 2159 2275 2888 3403 5430 1678 Size: 481 116 613 515 2027 2531 Fragments arranged by size: 2531 2027 613 515 481 116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2540 2572 2852 2884 2921 Size: 32 135 32 968 32 280 32 37 Cuts at: 2921 2953 3464 3496 3512 3544 4924 4956 4998 Size: 32 511 32 16 32 1380 32 42 Cuts at: 4998 5030 1373 Size: 32 2626 Fragments arranged by size: 2626 1380 968 511 280 135 42 37 32 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 3627 3873 669 Size: 988 281 1689 246 3079 Fragments arranged by size: 3079 1689 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6283 HpaI GTT'AAC Cuts at: 3873 3873 Size: 6283 KasI G'GCGC_C Cuts at: 2271 2884 3399 2271 Size: 613 515 5155 Fragments arranged by size: 5155 613 515 KpnI G_GTAC'C Cuts at: 3608 3608 Size: 6283 MfeI C'AATT_G Cuts at: 3882 3882 Size: 6283 MluI A'CGCG_T Cuts at: 6159 6159 Size: 6283 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2235 2583 3462 5397 5581 1305 Size: 930 348 879 1935 184 2007 Fragments arranged by size: 2007 1935 930 879 348 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2367 2409 10 Size: 54 1425 343 535 42 3884 Fragments arranged by size: 3884 1425 535 343 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2687 3344 4241 4600 4759 518 Size: 648 843 678 657 897 359 159 2042 Fragments arranged by size: 2042 897 843 678 657 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2136 2191 2249 2673 2780 3194 3699 4376 Size: 530 55 58 424 107 414 505 677 Cuts at: 4376 5524 5769 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 530 505 424 414 245 107 58 55 NarI GG'CG_CC Cuts at: 2272 2885 3400 2272 Size: 613 515 5155 Fragments arranged by size: 5155 613 515 NcoI C'CATG_G Cuts at: 513 1065 2631 513 Size: 552 1566 4165 Fragments arranged by size: 4165 1566 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6283 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6283 NotI GC'GGCC_GC Cuts at: 2757 3730 2757 Size: 973 5310 NspI r_CATG'y Cuts at: 1132 1229 3641 5186 1132 Size: 97 2412 1545 2229 Fragments arranged by size: 2412 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6283 PciI A'CATG_T Cuts at: 5182 5182 Size: 6283 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6283 PmeI GTTT'AAAC Cuts at: 3587 3587 Size: 6283 PmlI CAC'GTG Cuts at: 2377 2377 Size: 6283 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4352 4384 5865 5897 4352 Size: 32 1481 32 4738 Fragments arranged by size: 4738 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6283 PsiI TTA'TAA Cuts at: 3853 3853 Size: 6283 PspOMI G'GGCC_C Cuts at: 2116 2116 Size: 6283 PspXI vC'TCGA_Gb Cuts at: 2828 2828 Size: 6283 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2630 2662 2630 Size: 32 6251 PstI C_TGCA'G Cuts at: 830 2244 3535 3635 830 Size: 1414 1291 100 3478 Fragments arranged by size: 3478 1414 1291 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6283 PvuII CAG'CTG Cuts at: 2191 2673 3194 2191 Size: 482 521 5280 Fragments arranged by size: 5280 521 482 SacII CC_GC'GG Cuts at: 2250 2781 2250 Size: 531 5752 SalI G'TCGA_C Cuts at: 1936 3625 1936 Size: 1689 4594 SapI GCTCTTCn'nnn_ Cuts at: 2672 2672 Size: 6283 SbfI CC_TGCA'GG Cuts at: 2244 3635 2244 Size: 1391 4892 ScaI AGT'ACT Cuts at: 2834 4531 6243 2834 Size: 1697 1712 2874 Fragments arranged by size: 2874 1712 1697 SfcI C'TryA_G Cuts at: 826 1045 2240 3531 3631 4766 5447 5638 826 Size: 219 1195 1291 100 1135 681 191 1471 Fragments arranged by size: 1471 1291 1195 1135 681 219 191 100 SfoI GGC'GCC Cuts at: 2273 2886 3401 2273 Size: 613 515 5155 Fragments arranged by size: 5155 613 515 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6283 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6283 SmaI CCC'GGG Cuts at: 1872 2115 2220 2328 2643 3610 1872 Size: 243 105 108 315 967 4545 Fragments arranged by size: 4545 967 315 243 108 105 SmlI C'TyrA_G Cuts at: 820 1017 1943 2828 3062 3110 4387 5288 5550 Size: 197 926 885 234 48 1277 901 262 Cuts at: 5550 820 Size: 1553 Fragments arranged by size: 1553 1277 926 901 885 262 234 197 48 SnaBI TAC'GTA Cuts at: 493 4074 493 Size: 3581 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6283 SphI G_CATG'C Cuts at: 1132 3641 1132 Size: 2509 3774 SspI AAT'ATT Cuts at: 5 52 4207 5 Size: 47 4155 2081 Fragments arranged by size: 4155 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6283 StyI C'CwwG_G Cuts at: 513 1065 2074 2431 2631 3331 3722 513 Size: 552 1009 357 200 700 391 3074 Fragments arranged by size: 3074 1009 700 552 391 357 200 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2162 3149 4310 4327 4480 4665 5110 Size: 1255 582 987 1161 17 153 185 445 Cuts at: 5110 325 Size: 1498 Fragments arranged by size: 1498 1255 1161 987 582 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 4529 6241 Size: 274 80 33 51 1443 855 1697 1712 Cuts at: 6241 96 Size: 138 Fragments arranged by size: 1712 1697 1443 855 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6243 6275 6243 Size: 32 6251 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6283 XbaI T'CTAG_A Cuts at: 3538 3619 3538 Size: 81 6202 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3237 3237 Size: 6283 XhoI C'TCGA_G Cuts at: 1943 2828 1943 Size: 885 5398 XmaI C'CCGG_G Cuts at: 1870 2113 2218 2326 2641 3608 1870 Size: 243 105 108 315 967 4545 Fragments arranged by size: 4545 967 315 243 108 105 XmnI GAAnn'nnTTC Cuts at: 4412 4412 Size: 6283 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4091 276 Size: 53 83 186 936 2557 2468 Fragments arranged by size: 2557 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI RsrII SacI SanDI SexAI SfiI SrfI SwaI