(Linear) (Six-base) MAPSORT of: KIEE4128.seq Check: 565 from: 1 to: 1578 KIEE4128.orf With 182 enzymes: SgfI * March 22, 2012 17:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 374 1014 1578 Size: 374 640 564 Fragments arranged by size: 640 564 374 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 648 1578 Size: 648 930 AhdI GACnn_n'nnGTC Cuts at: 0 930 1578 Size: 930 648 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1036 1153 1578 Size: 1036 117 425 Fragments arranged by size: 1036 425 117 ApaI G_GGCC'C Cuts at: 0 115 1578 Size: 115 1463 ApoI r'AATT_y Cuts at: 0 301 1578 Size: 301 1277 AscI GG'CGCG_CC Cuts at: 0 1066 1578 Size: 1066 512 AvaI C'yCGr_G Cuts at: 0 108 213 321 636 727 823 1499 1578 Size: 108 105 108 315 91 96 676 79 Fragments arranged by size: 676 315 108 108 105 96 91 79 AvrII C'CTAG_G Cuts at: 0 69 426 1326 1578 Size: 69 357 900 252 Fragments arranged by size: 900 357 252 69 BamHI G'GATC_C Cuts at: 0 125 1578 Size: 125 1453 BanI G'GyrC_C Cuts at: 0 266 317 879 1281 1394 1578 Size: 266 51 562 402 113 184 Fragments arranged by size: 562 402 266 184 113 51 BanII G_rGCy'C Cuts at: 0 115 1578 Size: 115 1463 BbeI G_GCGC'C Cuts at: 0 270 883 1398 1578 Size: 270 613 515 180 Fragments arranged by size: 613 515 270 180 BbsI GAAGACnn'nnnn_ Cuts at: 0 384 1578 Size: 384 1194 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 981 1015 1578 Size: 981 34 563 Fragments arranged by size: 981 563 34 BglI GCCn_nnn'nGGC Cuts at: 0 745 760 791 1010 1578 Size: 745 15 31 219 568 Fragments arranged by size: 745 568 219 31 15 BglII A'GATC_T Cuts at: 0 548 1530 1578 Size: 548 982 48 Fragments arranged by size: 982 548 48 BlpI GC'TnA_GC Cuts at: 0 187 1456 1578 Size: 187 1269 122 Fragments arranged by size: 1269 187 122 Bme1580I G_kGCm'C Cuts at: 0 115 220 541 1246 1578 Size: 115 105 321 705 332 Fragments arranged by size: 705 332 321 115 105 BmgBI CAC'GTC Cuts at: 0 558 1206 1578 Size: 558 648 372 Fragments arranged by size: 648 558 372 BmrI ACTGGGnnnn_n' Cuts at: 0 429 878 985 1544 1578 Size: 429 449 107 559 34 Fragments arranged by size: 559 449 429 107 34 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1120 1578 Size: 1120 458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 490 1429 1510 1578 Size: 490 939 81 68 Fragments arranged by size: 939 490 81 68 Bpu10I CC'TnA_GC Cuts at: 0 334 1578 Size: 334 1244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1042 1090 1578 Size: 1042 48 488 Fragments arranged by size: 1042 488 48 BsaAI yAC'GTr Cuts at: 0 372 1578 Size: 372 1206 BsaHI Gr'CG_yC Cuts at: 0 247 267 819 880 1395 1578 Size: 247 20 552 61 515 183 Fragments arranged by size: 552 515 247 183 61 20 BsaWI w'CCGG_w Cuts at: 0 41 1578 Size: 41 1537 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 535 565 1363 1393 1507 1537 1578 Size: 535 30 798 30 114 30 41 Fragments arranged by size: 798 535 114 41 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 93 268 1213 1578 Size: 93 175 945 365 Fragments arranged by size: 945 365 175 93 BseYI C'CCAG_C Cuts at: 0 225 485 1092 1224 1578 Size: 225 260 607 132 354 Fragments arranged by size: 607 354 260 225 132 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 328 352 580 1042 1578 Size: 328 24 228 462 536 Fragments arranged by size: 536 462 328 228 24 BsiEI CG_ry'CG Cuts at: 0 315 755 1578 Size: 315 440 823 Fragments arranged by size: 823 440 315 BsiHKAI G_wGCw'C Cuts at: 0 85 377 692 1578 Size: 85 292 315 886 Fragments arranged by size: 886 315 292 85 BsmBI CGTCTCn'nnnn_ Cuts at: 0 678 1578 Size: 678 900 Bsp1286I G_dGCh'C Cuts at: 0 85 115 220 377 541 692 1246 1578 Size: 85 30 105 157 164 151 554 332 Fragments arranged by size: 554 332 164 157 151 105 85 30 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 374 1014 1578 Size: 374 640 564 Fragments arranged by size: 640 564 374 BsrBI CCG'CTC Cuts at: 0 912 1578 Size: 912 666 BsrFI r'CCGG_y Cuts at: 0 314 1391 1578 Size: 314 1077 187 Fragments arranged by size: 1077 314 187 BssHII G'CGCG_C Cuts at: 0 191 193 195 205 295 458 460 574 Size: 191 2 2 10 90 163 2 114 Cuts at: 574 589 1066 1578 Size: 15 477 512 Fragments arranged by size: 512 477 191 163 114 90 15 10 2 2 2 BstXI CCAn_nnnn'nTGG Cuts at: 0 684 1232 1578 Size: 684 548 346 Fragments arranged by size: 684 548 346 BstYI r'GATC_y Cuts at: 0 125 548 1530 1578 Size: 125 423 982 48 Fragments arranged by size: 982 423 125 48 Bsu36I CC'TnA_GG Cuts at: 0 1314 1578 Size: 1314 264 BtgI C'CryG_G Cuts at: 0 242 626 758 773 1262 1578 Size: 242 384 132 15 489 316 Fragments arranged by size: 489 384 316 242 132 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 684 822 1578 Size: 684 138 756 Fragments arranged by size: 756 684 138 EaeI y'GGCC_r Cuts at: 0 360 402 597 752 770 1002 1578 Size: 360 42 195 155 18 232 576 Fragments arranged by size: 576 360 232 195 155 42 18 EagI C'GGCC_G Cuts at: 0 752 1578 Size: 752 826 EarI CTCTTCn'nnn_ Cuts at: 0 667 1578 Size: 667 911 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1021 1578 Size: 1021 557 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 490 648 1429 1510 1578 Size: 490 158 781 81 68 Fragments arranged by size: 781 490 158 81 68 EcoNI CCTnn'n_nnAGG Cuts at: 0 1012 1578 Size: 1012 566 EcoO109I rG'GnC_Cy Cuts at: 0 112 423 1545 1578 Size: 112 311 1122 33 Fragments arranged by size: 1122 311 112 33 EcoRI G'AATT_C Cuts at: 0 301 1578 Size: 301 1277 FspI TGC'GCA Cuts at: 0 1042 1423 1578 Size: 1042 381 155 Fragments arranged by size: 1042 381 155 FspAI rTGC'GCAy Cuts at: 0 1042 1578 Size: 1042 536 HaeII r_GCGC'y Cuts at: 0 154 270 883 1398 1578 Size: 154 116 613 515 180 Fragments arranged by size: 613 515 180 154 116 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 535 567 847 879 916 948 1459 1491 Size: 535 32 280 32 37 32 511 32 Cuts at: 1491 1507 1539 1578 Size: 16 32 39 Fragments arranged by size: 535 511 280 39 37 32 32 32 32 32 16 KasI G'GCGC_C Cuts at: 0 266 879 1394 1578 Size: 266 613 515 184 Fragments arranged by size: 613 515 266 184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 230 578 1457 1578 Size: 230 348 879 121 Fragments arranged by size: 879 348 230 121 MscI TGG'CCA Cuts at: 0 362 404 1578 Size: 362 42 1174 Fragments arranged by size: 1174 362 42 MslI CAynn'nnrTG Cuts at: 0 682 1339 1578 Size: 682 657 239 Fragments arranged by size: 682 657 239 MspA1I CmG'CkG Cuts at: 0 131 186 244 668 775 1189 1578 Size: 131 55 58 424 107 414 389 Fragments arranged by size: 424 414 389 131 107 58 55 NarI GG'CG_CC Cuts at: 0 267 880 1395 1578 Size: 267 613 515 183 Fragments arranged by size: 613 515 267 183 NcoI C'CATG_G Cuts at: 0 626 1578 Size: 626 952 NotI GC'GGCC_GC Cuts at: 0 752 1578 Size: 752 826 PmlI CAC'GTG Cuts at: 0 372 1578 Size: 372 1206 PspOMI G'GGCC_C Cuts at: 0 111 1578 Size: 111 1467 PspXI vC'TCGA_Gb Cuts at: 0 823 1578 Size: 823 755 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 625 657 1578 Size: 625 32 921 Fragments arranged by size: 921 625 32 PstI C_TGCA'G Cuts at: 0 239 1530 1578 Size: 239 1291 48 Fragments arranged by size: 1291 239 48 PvuII CAG'CTG Cuts at: 0 186 668 1189 1578 Size: 186 482 521 389 Fragments arranged by size: 521 482 389 186 SacII CC_GC'GG Cuts at: 0 245 776 1578 Size: 245 531 802 Fragments arranged by size: 802 531 245 SapI GCTCTTCn'nnn_ Cuts at: 0 667 1578 Size: 667 911 SbfI CC_TGCA'GG Cuts at: 0 239 1578 Size: 239 1339 ScaI AGT'ACT Cuts at: 0 829 1578 Size: 829 749 SfcI C'TryA_G Cuts at: 0 235 1526 1578 Size: 235 1291 52 Fragments arranged by size: 1291 235 52 SfoI GGC'GCC Cuts at: 0 268 881 1396 1578 Size: 268 613 515 182 Fragments arranged by size: 613 515 268 182 SmaI CCC'GGG Cuts at: 0 110 215 323 638 1578 Size: 110 105 108 315 940 Fragments arranged by size: 940 315 110 108 105 SmlI C'TyrA_G Cuts at: 0 823 1057 1105 1578 Size: 823 234 48 473 Fragments arranged by size: 823 473 234 48 StyI C'CwwG_G Cuts at: 0 69 426 626 1326 1578 Size: 69 357 200 700 252 Fragments arranged by size: 700 357 252 200 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 157 1144 1578 Size: 157 987 434 Fragments arranged by size: 987 434 157 TatI w'GTAC_w Cuts at: 0 827 1578 Size: 827 751 XbaI T'CTAG_A Cuts at: 0 1533 1578 Size: 1533 45 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1232 1578 Size: 1232 346 XhoI C'TCGA_G Cuts at: 0 823 1578 Size: 823 755 XmaI C'CCGG_G Cuts at: 0 108 213 321 636 1578 Size: 108 105 108 315 942 Fragments arranged by size: 942 315 108 108 105 Enzymes that do cut: AarI AcuI AhdI AlfI ApaI ApoI AscI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstXI BstYI Bsu36I BtgI BtgZI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI NotI PmlI PspOMI PspXI PsrI PstI PvuII SacII SapI SbfI ScaI SfcI SfoI SmaI SmlI StyI TaqII TatI XbaI XcmI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AloI AlwNI ApaLI AseI AsiSI BaeI BbvCI BciVI BclI BfrBI BmtI BsaI BsaBI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRV FalI FseI HincII HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PvuI RsrII SacI SalI SanDI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XmnI ZraI