################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AlwI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BbvCI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BseRI BsgI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I ClaI CpoI DpnII DraI EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MluNI MscI MseI MslI MunI NcoI NdeI NdeII NheI NruI NsiI NspV PacI PflFI PinAI PmeI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XhoII XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccIII 143 TCCGGA T^CCGGA AhdI 116 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 971 GGGCCC GGGCC^C AspEI 116 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 874 CACGTG CAC^GTG BcgI 762 CGAGCGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 205 GCTGAGC GC^T[ACGT]AGC Bpu1102I 205 GCTGAGC GC^T[ACGT]AGC BsaWI 143 TCCGGA [AT]^CCGG[AT] BseAI 143 TCCGGA T^CCGGA BspEI 143 TCCGGA T^CCGGA BspMI 53 ACCTGC ACCTGC(4/8) BsrFI 220 GCCGGC [GA]^CCGG[CT] CelII 205 GCTGAGC GC^T[ACGT]AGC Cfr10I 220 GCCGGC [GA]^CCGG[CT] DdeI 206 CTGAG C^T[ACGT]AG DraII 970 GGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 42 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 453 GACGTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 116 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 970 GGGGCCC [GA]G^G[ACGT]CC[CT] MroI 143 TCCGGA T^CCGGA MspA1I 355 CCGCTG C[AC]G^C[GT]G NaeI 220 GCCGGC GCC^GGC NgoMIV 220 GCCGGC G^CCGGC NlaIII 330 CATG CATG^ NotI 104 GCGGCCGC GC^GGCCGC NspI 329 GCATGT [GA]CATG^[CT] PflMI 375 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 874 CACGTG CAC^GTG PmlI 874 CACGTG CAC^GTG PshAI 360 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfaNI 350 GCATC GCATC(5/9) SfiI 513 GGCCCTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Tsp45I 367 GTCAC ^GT[GC]AC Van91I 375 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AfaI 285 GTAC GT^AC 305 GTAC Alw44I 39 GTGCAC G^TGCAC 877 GTGCAC AlwNI 112 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG 376 CAGAGCCTG ApaLI 39 GTGCAC G^TGCAC 877 GTGCAC AspHI 39 GTGCAC G[AT]GC[AT]^C 877 GTGCAC BglI 505 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 514 GCCCTCGGGGC BsaAI 658 TACGTG [CT]AC^GT[GA] 874 CACGTG BsiHKAI 39 GTGCAC G[AT]GC[AT]^C 877 GTGCAC BsrBI 843 CCGCTC CCGCTC(-3/-3) 627 GAGCGG BsrI 755 ACTGG ACTGG(1/-1) 228 CCAGT BssHII 18 GCGCGC G^CGCGC 765 GCGCGC BstF5I 351 CATCC GGATG(2/0) 681 CATCC FokI 351 CATCC GGATG(9/13) 681 CATCC HgaI 79 GACGC GACGC(5/10) 360 GACGC HinfI 88 GAGTC G^A[ACGT]TC 310 GACTC MaeIII 50 GTTAC ^GT[ACGT]AC 367 GTCAC PaeR7I 85 CTCGAG C^TCGAG 927 CTCGAG PleI 310 GACTC GAGTC(4/5) 88 GAGTC PstI 622 CTGCAG CTGCA^G 640 CTGCAG RsaI 285 GTAC GT^AC 305 GTAC SexAI 473 ACCTGGT A^CC[AT]GGT 719 ACCTGGT SfcI 622 CTGCAG C^T[GA][CT]AG 640 CTGCAG SmaI 795 CCCGGG CCC^GGG 933 CCCGGG SmlI 85 CTCGAG C^T[CT][GA]AG 927 CTCGAG TspRI 229 CAGTG CA[GC]TG[ACGT][ACGT]^ 620 CACTG XcmI 428 CCAAGTGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 464 CCACGGAGAACCTGG XhoI 85 CTCGAG C^TCGAG 927 CTCGAG XmaCI 795 CCCGGG C^CCGGG 933 CCCGGG XmaI 795 CCCGGG C^CCGGG 933 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AcyI AluI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaJI BsiEI BsiYI BslI BsoBI Bsp1286I BssKI BstNI BstUI Cac8I CfoI Cfr13I DsaI EaeI EagI EclXI Eco52I EcoRII FauI Fnu4HI HaeII HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI KasI MaeII MboII MnlI MspI MvaI MvnI MwoI NarI NciI NlaIV Sau96I ScrFI SfoI TaqI TseI TthHB8I