(Circular) (Six-base) MAPSORT of: pFN21AE5015.seq Check: 3612 from: 1 to: 5695 pFN21AE5015 5695 bp With 182 enzymes: SgfI * December 8, 2014 13:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3505 278 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 1937 Size: 1101 4594 Acc65I G'GTAC_C Cuts at: 3016 3016 Size: 5695 AclI AA'CG_TT Cuts at: 1563 3822 4195 1563 Size: 2259 373 3063 Fragments arranged by size: 3063 2259 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2656 3758 5142 5410 1632 Size: 1024 1102 1384 268 1917 Fragments arranged by size: 1917 1384 1102 1024 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5498 AflIII A'CryG_T Cuts at: 1203 4594 5571 1203 Size: 3391 977 1327 Fragments arranged by size: 3391 1327 977 AgeI A'CCGG_T Cuts at: 4526 4526 Size: 5695 AhdI GACnn_n'nnGTC Cuts at: 2126 4424 2126 Size: 2298 3397 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5695 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5695 AlwNI CAG_nnn'CTG Cuts at: 2122 2386 3438 5010 2122 Size: 264 1052 1572 2807 Fragments arranged by size: 2807 1572 1052 264 ApaI G_GGCC'C Cuts at: 2980 2980 Size: 5695 ApaLI G'TGCA_C Cuts at: 2044 2882 3752 4908 2044 Size: 838 870 1156 2831 Fragments arranged by size: 2831 1156 870 838 ApoI r'AATT_y Cuts at: 1493 3004 3230 3382 5491 1493 Size: 1511 226 152 2109 1697 Fragments arranged by size: 2109 1697 1511 226 152 AseI AT'TA_AT Cuts at: 160 1032 4249 160 Size: 872 3217 1606 Fragments arranged by size: 3217 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2522 2765 2800 2810 2932 2938 Size: 73 147 432 243 35 10 122 6 Cuts at: 2938 3020 1870 Size: 82 4545 Fragments arranged by size: 4545 432 243 147 122 82 73 35 10 6 BamHI G'GATC_C Cuts at: 3025 3392 3025 Size: 367 5328 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2415 2711 2806 3016 4471 Size: 325 217 627 628 296 95 210 1455 Cuts at: 4471 618 Size: 1842 Fragments arranged by size: 1842 1455 628 627 325 296 217 210 95 BanII G_rGCy'C Cuts at: 1391 2217 2700 2861 2939 2980 3014 1391 Size: 826 483 161 78 41 34 4072 Fragments arranged by size: 4072 826 483 161 78 41 34 BbeI G_GCGC'C Cuts at: 2419 2715 2810 2419 Size: 296 95 5304 Fragments arranged by size: 5304 296 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2071 2320 2863 928 Size: 971 172 249 543 3760 Fragments arranged by size: 3760 971 543 249 172 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2756 2790 3886 3920 1903 Size: 34 50 34 735 34 1096 34 3678 Fragments arranged by size: 3678 1096 735 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3587 4797 3587 Size: 1210 4485 BclI T'GATC_A Cuts at: 1548 1548 Size: 5695 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2516 2525 4306 Size: 107 122 71 631 605 844 9 1781 Cuts at: 4306 136 Size: 1525 Fragments arranged by size: 1781 1525 844 631 605 122 107 71 9 BglII A'GATC_T Cuts at: 5690 5690 Size: 5695 BlpI GC'TnA_GC Cuts at: 2211 3112 2211 Size: 901 4794 Bme1580I G_kGCm'C Cuts at: 1790 2048 2886 2980 3756 4912 1790 Size: 258 838 94 776 1156 2573 Fragments arranged by size: 2573 1156 838 776 258 94 BmgBI CAC'GTC Cuts at: 1318 2460 1318 Size: 1142 4553 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3426 4384 442 Size: 416 53 83 399 24 2009 958 1753 Fragments arranged by size: 2009 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5695 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2210 2912 4355 1239 Size: 950 21 702 1443 2579 Fragments arranged by size: 2579 1443 950 702 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3820 4685 4983 3820 Size: 865 298 4532 Fragments arranged by size: 4532 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4358 882 Size: 617 2859 2219 Fragments arranged by size: 2859 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2665 2881 3486 493 Size: 711 1461 216 605 2702 Fragments arranged by size: 2702 1461 711 605 216 BsaBI GATnn'nnATC Cuts at: 5689 5689 Size: 5695 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2416 2712 2807 3502 Size: 53 83 186 936 883 296 95 695 Cuts at: 3502 3884 4519 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 883 695 635 382 296 186 95 83 53 BsaWI w'CCGG_w Cuts at: 2148 4128 4526 4800 4947 5358 2148 Size: 1980 398 274 147 411 2485 Fragments arranged by size: 2485 1980 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5470 Fragments arranged by size: 5470 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5695 BseYI C'CCAG_C Cuts at: 2786 4898 5394 2786 Size: 2112 496 3087 Fragments arranged by size: 3087 2112 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2031 2113 2172 2230 3145 3906 Size: 275 186 30 82 59 58 915 761 Cuts at: 3906 4934 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 915 761 275 186 82 59 58 30 BsiHKAI G_wGCw'C Cuts at: 2048 2886 3756 3841 4912 2048 Size: 838 870 85 1071 2831 Fragments arranged by size: 2831 1071 870 838 85 BsmI GAATG_Cn' Cuts at: 3206 3299 3206 Size: 93 5602 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2217 2700 2861 2886 2939 2980 Size: 399 258 169 483 161 25 53 41 Cuts at: 2980 3014 3756 3841 4912 1391 Size: 34 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 483 399 258 169 161 85 53 41 34 25 BspEI T'CCGG_A Cuts at: 2148 5358 2148 Size: 3210 2485 BspHI T'CATG_A Cuts at: 3584 5314 3584 Size: 1730 3965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2067 3050 844 Size: 1223 983 3489 Fragments arranged by size: 3489 1223 983 BsrBI CCG'CTC Cuts at: 1394 1823 2634 2850 3582 1394 Size: 429 811 216 732 3507 Fragments arranged by size: 3507 811 732 429 216 BsrDI GCAATG_nn' Cuts at: 65 1248 4190 4364 65 Size: 1183 2942 174 1396 Fragments arranged by size: 2942 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2225 4339 4526 1700 Size: 110 415 2114 187 2869 Fragments arranged by size: 2869 2114 415 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5695 BssHII G'CGCG_C Cuts at: 1925 2023 2770 1925 Size: 98 747 4850 Fragments arranged by size: 4850 747 98 BssSI C'ACGA_G Cuts at: 3755 4767 3755 Size: 1012 4683 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5695 BstBI TT'CG_AA Cuts at: 4499 4499 Size: 5695 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5695 BstYI r'GATC_y Cuts at: 1972 3025 3392 3777 3794 5246 5332 5344 5690 Size: 1053 367 385 17 1452 86 12 346 Cuts at: 5690 1972 Size: 1977 Fragments arranged by size: 1977 1452 1053 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2469 2818 2853 513 Size: 552 1404 349 35 3355 Fragments arranged by size: 3355 1404 552 349 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4956 Fragments arranged by size: 4956 683 56 BtsI GCAGTG_nn' Cuts at: 2624 3211 4005 4025 2624 Size: 587 794 20 4294 Fragments arranged by size: 4294 794 587 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5695 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5660 DraI TTT'AAA Cuts at: 2999 2999 Size: 5695 DraIII CAC_nnn'GTG Cuts at: 2052 2052 Size: 5695 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2464 4702 809 Size: 507 1148 2238 1802 Fragments arranged by size: 2238 1802 1148 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2110 2169 Size: 54 1425 50 275 18 198 82 59 Cuts at: 2169 2227 2293 2430 2487 3142 4031 5660 8 Size: 58 66 137 57 655 889 1629 43 Fragments arranged by size: 1629 1425 889 655 275 198 137 82 66 59 58 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2028 2110 2169 2227 3142 1537 Size: 275 216 82 59 58 915 4090 Fragments arranged by size: 4090 915 275 216 82 59 58 EarI CTCTTCn'nnn_ Cuts at: 1353 3625 1353 Size: 2272 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2117 2851 4282 4656 4802 387 Size: 1064 138 528 734 1431 374 146 1280 Fragments arranged by size: 1431 1280 1064 734 528 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2210 2656 2912 3758 4355 5142 Size: 393 557 21 446 256 846 597 787 Cuts at: 5142 5410 1239 Size: 268 1524 Fragments arranged by size: 1524 846 787 597 557 446 393 268 256 21 EcoO109I rG'GnC_Cy Cuts at: 2976 2976 Size: 5695 EcoRI G'AATT_C Cuts at: 3004 3382 3004 Size: 378 5317 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2179 2179 Size: 5695 FspI TGC'GCA Cuts at: 4201 4201 Size: 5695 HaeII r_GCGC'y Cuts at: 1678 2073 2145 2197 2204 2419 2645 2715 2757 Size: 395 72 52 7 215 226 70 42 Cuts at: 2757 2810 4842 1678 Size: 53 2032 2531 Fragments arranged by size: 2531 2032 395 226 215 72 70 53 52 42 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2112 2144 4336 4368 4410 Size: 32 135 32 540 32 2192 32 42 Cuts at: 4410 4442 1373 Size: 32 2626 Fragments arranged by size: 2626 2192 540 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3039 3285 669 Size: 988 281 1101 246 3079 Fragments arranged by size: 3079 1101 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5695 HpaI GTT'AAC Cuts at: 3285 3285 Size: 5695 KasI G'GCGC_C Cuts at: 2415 2711 2806 2415 Size: 296 95 5304 Fragments arranged by size: 5304 296 95 KpnI G_GTAC'C Cuts at: 3020 3020 Size: 5695 MfeI C'AATT_G Cuts at: 3294 3294 Size: 5695 MluI A'CGCG_T Cuts at: 5571 5571 Size: 5695 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4809 4993 1305 Size: 3504 184 2007 Fragments arranged by size: 3504 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3873 Fragments arranged by size: 3873 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3653 4012 4171 518 Size: 648 2487 359 159 2042 Fragments arranged by size: 2487 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2362 3111 3788 4936 5181 1606 Size: 756 749 677 1148 245 2120 Fragments arranged by size: 2120 1148 756 749 677 245 NaeI GCC'GGC Cuts at: 2227 2227 Size: 5695 NarI GG'CG_CC Cuts at: 2416 2712 2807 2416 Size: 296 95 5304 Fragments arranged by size: 5304 296 95 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5143 NdeI CA'TA_TG Cuts at: 387 387 Size: 5695 NgoMIV G'CCGG_C Cuts at: 2225 2225 Size: 5695 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5695 NotI GC'GGCC_GC Cuts at: 2110 3142 2110 Size: 1032 4663 NspI r_CATG'y Cuts at: 1132 1229 2338 3053 4598 1132 Size: 97 1109 715 1545 2229 Fragments arranged by size: 2229 1545 1109 715 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5695 PciI A'CATG_T Cuts at: 4594 4594 Size: 5695 PflMI CCAn_nnn'nTGG Cuts at: 1108 2386 1108 Size: 1278 4417 PmeI GTTT'AAAC Cuts at: 2999 2999 Size: 5695 PmlI CAC'GTG Cuts at: 2881 2881 Size: 5695 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3764 3796 5277 5309 3764 Size: 32 1481 32 4150 Fragments arranged by size: 4150 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2369 1613 Size: 756 4939 PsiI TTA'TAA Cuts at: 3265 3265 Size: 5695 PspOMI G'GGCC_C Cuts at: 2976 2976 Size: 5695 PspXI vC'TCGA_Gb Cuts at: 2090 2932 2090 Size: 842 4853 PstI C_TGCA'G Cuts at: 830 2631 2649 3047 830 Size: 1801 18 398 3478 Fragments arranged by size: 3478 1801 398 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5695 SalI G'TCGA_C Cuts at: 1936 3037 1936 Size: 1101 4594 SbfI CC_TGCA'GG Cuts at: 3047 3047 Size: 5695 ScaI AGT'ACT Cuts at: 3943 5655 3943 Size: 1712 3983 SexAI A'CCwGG_T Cuts at: 2478 2724 2478 Size: 246 5449 SfcI C'TryA_G Cuts at: 826 1045 2627 2645 3043 4178 4859 5050 826 Size: 219 1582 18 398 1135 681 191 1471 Fragments arranged by size: 1582 1471 1135 681 398 219 191 18 SfiI GGCCn_nnn'nGGCC Cuts at: 2525 2525 Size: 5695 SfoI GGC'GCC Cuts at: 2417 2713 2808 2417 Size: 296 95 5304 Fragments arranged by size: 5304 296 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5695 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5695 SmaI CCC'GGG Cuts at: 1872 2802 2940 3022 1872 Size: 930 138 82 4545 Fragments arranged by size: 4545 930 138 82 SmlI C'TyrA_G Cuts at: 820 1017 1943 2090 2932 3799 4700 4962 820 Size: 197 926 147 842 867 901 262 1553 Fragments arranged by size: 1553 926 901 867 842 262 197 147 SnaBI TAC'GTA Cuts at: 493 3486 493 Size: 2993 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5695 SphI G_CATG'C Cuts at: 1132 3053 1132 Size: 1921 3774 SspI AAT'ATT Cuts at: 5 52 3619 5 Size: 47 3567 2081 Fragments arranged by size: 3567 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5695 StyI C'CwwG_G Cuts at: 513 1065 3134 513 Size: 552 2069 3074 Fragments arranged by size: 3074 2069 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2800 3722 3739 3892 4077 4522 325 Size: 1255 1220 922 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1220 922 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3941 5653 96 Size: 274 80 33 51 1443 1964 1712 138 Fragments arranged by size: 1964 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5655 5687 5655 Size: 32 5663 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5695 XbaI T'CTAG_A Cuts at: 3031 3031 Size: 5695 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2440 2476 2440 Size: 36 5659 XhoI C'TCGA_G Cuts at: 1943 2090 2932 1943 Size: 147 842 4706 Fragments arranged by size: 4706 842 147 XmaI C'CCGG_G Cuts at: 1870 2800 2938 3020 1870 Size: 930 138 82 4545 Fragments arranged by size: 4545 930 138 82 XmnI GAAnn'nnTTC Cuts at: 3824 3824 Size: 5695 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3503 276 Size: 53 83 186 936 1969 2468 Fragments arranged by size: 2468 1969 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PpuMI PsrI PvuII RsrII SacI SacII SanDI SapI SrfI SwaI