################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraI DraII DraIII DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I TspRI Tth111I Van91I XbaI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AciI 64 GCGG CCGC(-3/-1) AhdI 48 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 89 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG AspEI 48 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 139 CTCGAG C^[CT]CG[GA]G BbvI 206 GCAGC GCAGC(8/12) BpmI 116 CTGGAG CTGGAG(16/14) BsaJI 78 CCAGGG C^C[ACGT][ACGT]GG BsaWI 44 ACCGGA [AT]^CCGG[AT] BsmI 60 GAATGC GAATGC(1/-1) BsoBI 139 CTCGAG C^[CT]CG[GA]G BssKI 78 CCAGG ^CC[ACGT]GG BstF5I 75 CATCC GGATG(2/0) BstNI 78 CCAGG CC^[AT]GG CfoI 170 GCGC GCG^C DdeI 87 CTCAG C^T[ACGT]AG DrdI 216 GACCACCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 48 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRII 78 CCAGG ^CC[AT]GG Fnu4HI 206 GCAGC GC^[ACGT]GC FokI 75 CATCC GGATG(9/13) HaeII 169 AGCGCC [GA]GCGC^[CT] HaeIII 52 GGCC GG^CC HapII 45 CCGG C^CGG HhaI 170 GCGC GCG^C HinP1I 170 GCGC G^CGC HpaII 45 CCGG C^CGG ItaI 206 GCAGC GC^[ACGT]GC MaeIII 41 GTGAC ^GT[ACGT]AC MspI 45 CCGG C^CGG MvaI 78 CCAGG CC^[AT]GG NlaIII 123 CATG CATG^ PaeR7I 139 CTCGAG C^TCGAG ScrFI 78 CCAGG CC^[ACGT]GG SmlI 139 CTCGAG C^T[CT][GA]AG TseI 206 GCAGC G^C[AT]GC Tsp45I 41 GTGAC ^GT[GC]AC XcmI 201 CCAGAGCAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 139 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AvaII 66 GGTCC G^G[AT]CC 215 GGACC BsiYI 38 CCTGTGACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 174 CCCAGACAAGG BslI 38 CCTGTGACCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 174 CCCAGACAAGG BsmAI 83 GAGAC GTCTC(1/5) 119 GAGAC BsmFI 214 GGGAC GGGAC(10/14) 67 GTCCC Cfr13I 66 GGTCC G^G[ACGT]CC 215 GGACC HinfI 13 GAGTC G^A[ACGT]TC 85 GACTC NlaIV 66 GGTCCC GG[ACGT]^[ACGT]CC 214 GGGACC PleI 85 GACTC GAGTC(4/5) 13 GAGTC Sau96I 66 GGTCC G^G[ACGT]CC 215 GGACC TaqI 11 TCGA T^CGA 140 TCGA TthHB8I 11 TCGA T^CGA 140 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### AluI Cac8I MnlI