################################## # NO CUT ENZYMES ( 192 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DpnII DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MboI MboII MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NciI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfcI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AfaI 39 GTAC GT^AC AluI 295 AGCT AG^CT Alw44I 84 GTGCAC G^TGCAC ApaLI 84 GTGCAC G^TGCAC BanII 294 GAGCTC G[GA]GC[CT]^C BbvI 302 GCAGC GCAGC(8/12) BcgI 360 CGAGACCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 184 GGATAC GTATCC(6/5) BglI 92 GCCCCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 189 CTGGAG CTGGAG(16/14) BsmFI 273 GGGAC GGGAC(10/14) BspMI 145 GCAGGT ACCTGC(4/8) BsrI 188 ACTGG ACTGG(1/-1) BstF5I 380 GGATG GGATG(2/0) BstXI 69 CCATGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 236 GCTGGC GC[ACGT]^[ACGT]GC CfoI 234 GCGC GCG^C DraII 90 AGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 69 CCATGG C^C[GA][CT]GG EcoO109I 90 AGGCCCC [GA]G^G[ACGT]CC[CT] FauI 44 GCGGG CCCGC(4/6) FokI 380 GGATG GGATG(9/13) HaeII 233 GGCGCT [GA]GCGC^[CT] HapII 288 CCGG C^CGG HgaI 275 GACGC GACGC(5/10) HhaI 234 GCGC GCG^C HinP1I 234 GCGC G^CGC HinfI 284 GAATC G^A[ACGT]TC HpaII 288 CCGG C^CGG MslI 70 CATGGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 288 CCGG C^CGG NcoI 69 CCATGG C^CATGG RsaI 39 GTAC GT^AC SacI 294 GAGCTC GAGCT^C SfiI 91 GGCCCCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 297 CTCAAG C^T[CT][GA]AG TaqI 17 TCGA T^CGA TfiI 284 GAATC G^A[AT]TC TseI 302 GCAGC G^C[AT]GC TthHB8I 17 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AspHI 84 GTGCAC G[AT]GC[AT]^C 294 GAGCTC BsaI 223 GAGACC GGTCTC(1/5) 361 GAGACC BsiHKAI 84 GTGCAC G[AT]GC[AT]^C 294 GAGCTC BsmAI 223 GAGAC GTCTC(1/5) 361 GAGAC Fnu4HI 11 GCCGC GC^[ACGT]GC 302 GCAGC ItaI 11 GCCGC GC^[ACGT]GC 302 GCAGC MspA1I 165 CAGCGG C[AC]G^C[GT]G 303 CAGCGG MwoI 92 GCCCCAAGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 296 GCTCAAGCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI BanI BmyI BsaJI Bsp1286I BssKI BstNI Cfr13I EcoRII EcoT14I HaeIII MnlI MvaI NlaIII NlaIV Sau96I ScrFI StyI