################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII AccIII AflII AgeI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspI AviII AvrII BamHI BbeI BbrPI BfrI BglI BglII BlnI BsaAI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstYI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FspI HaeII HindIII HpaI KasI MflI MluI MroI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XhoII ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccI 1159 GTAGAC GT^[AC][GT]AC AclI 1468 AACGTT AA^CGTT AcsI 1343 GAATTC [GA]^AATT[CT] AcyI 28 GACGCC G[GA]^CG[CT]C AflIII 11 ACGTGT A^C[GA][CT]GT AhdI 106 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 228 GGGCCC GGGCC^C ApoI 1343 GAATTC [GA]^AATT[CT] AspEI 106 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1008 TGGCCA TGG^CCA BbsI 1554 GTCTTC GAAGAC(2/6) BclI 384 TGATCA T^GATCA BlpI 1111 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1111 GCTAAGC GC^T[ACGT]AGC BpuAI 1554 GTCTTC GAAGAC(2/6) BsaBI 1231 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 28 GACGCC G[GA]^CG[CT]C BsmBI 670 CGTCTC CGTCTC(1/5) BspMI 234 GCAGGT ACCTGC(4/8) BsrBI 1752 CCGCTC CCGCTC(-3/-3) BssSI 1610 CTCGTG CACGAG(-5/-1) BstAPI 897 GCAGCAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1111 GCTAAGC GC^T[ACGT]AGC DsaI 198 CCGCGG C^C[GA][CT]GG EaeI 1008 TGGCCA [CT]^GGCC[GA] Eam1105I 106 GACAACGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1343 GAATTC G^AATTC EcoT14I 476 CCAAGG C^C[AT][AT]GG FbaI 384 TGATCA T^GATCA FseI 60 GGCCGGCC GGCCGG^CC Hin1I 28 GACGCC G[GA]^CG[CT]C KspI 198 CCGCGG CCGC^GG MamI 1231 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 901 CAATTG C^AATTG MluNI 1008 TGGCCA TGG^CCA MscI 1008 TGGCCA TGG^CCA MseI 1301 TTAA T^TAA MunI 901 CAATTG C^AATTG NaeI 61 GCCGGC GCC^GGC NgoMIV 61 GCCGGC G^CCGGC NspI 579 GCATGT [GA]CATG^[CT] PaeR7I 1144 CTCGAG C^TCGAG PflMI 351 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 154 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1468 AACGTT AA^CGTT SacII 198 CCGCGG CCGC^GG SapI 1063 GAAGAGC GCTCTTC(1/4) SmaI 145 CCCGGG CCC^GGG StuI 1745 AGGCCT AGG^CCT StyI 476 CCAAGG C^C[AT][AT]GG Tru9I 1301 TTAA T^TAA Van91I 351 CCAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 999 TCTAGA T^CTAGA XhoI 1144 CTCGAG C^TCGAG XmaCI 145 CCCGGG C^CCGGG XmaI 145 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 90 GGTACC G^GTACC 1433 GGTACC AlwNI 352 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG 404 CAGAAGCTG Asp718I 90 GGTACC G^GTACC 1433 GGTACC BbvCI 714 CCTCAGC CCTCAGC(-5/-2) 757 GCTGAGG BcgI 27 CGACGCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1580 CGAACCCTGTGC BciVI 565 GGATAC GTATCC(6/5) 1497 GTATCC BfaI 1000 CTAG C^TAG 1633 CTAG BsaI 1174 GAGACC GGTCTC(1/5) 1542 GAGACC BsaWI 42 ACCGGA [AT]^CCGG[AT] 1550 TCCGGT BsgI 540 GTGCAG GTGCAG(16/14) 988 GTGCAG BsiEI 201 CGGTCG CG[GA][CT]^CG 294 CGACCG BstEII 658 GGTTACC G^GT[ACGT]ACC 1278 GGTCACC BstPI 658 GGTTACC G^GT[ACGT]ACC 1278 GGTCACC BstXI 265 CCACTGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 648 CCAAGCAGCTGG DraII 152 AGGACCC [GA]G^G[ACGT]CC[CT] 159 AGGTCCC EcoO109I 152 AGGACCC [GA]G^G[ACGT]CC[CT] 159 AGGTCCC EcoO65I 658 GGTTACC G^GT[ACGT]ACC 1278 GGTCACC HincII 310 GTCAAC GT[CT]^[GA]AC 1138 GTCAAC HindII 310 GTCAAC GT[CT]^[GA]AC 1138 GTCAAC KpnI 90 GGTACC GGTAC^C 1433 GGTACC MaeI 1000 CTAG C^TAG 1633 CTAG MslI 355 CACCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1630 CACCTAGATG PleI 1054 GACTC GAGTC(4/5) 1410 GACTC PpuMI 152 AGGACCC [GA]G^G[AT]CC[CT] 159 AGGTCCC SacI 919 GAGCTC GAGCT^C 1349 GAGCTC SmlI 1144 CTCGAG C^T[CT][GA]AG 1693 CTCAAG TfiI 471 GATTC G^A[AT]TC 732 GATTC Tsp45I 1279 GTCAC ^GT[GC]AC 1613 GTGAC XcmI 120 CCAAGTCCGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 842 CCAGCAAGCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccII AciI AfaI AluI AlwI Asp700I AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp509I TspRI TthHB8I XmnI