(Circular) (Six-base) MAPSORT of: pFN21AE4162.seq Check: 3317 from: 1 to: 6526 pFN21AE4162 6526 bp With 182 enzymes: SgfI * March 28, 2012 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2230 2230 Size: 6526 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4336 278 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3165 3869 1937 Size: 1228 704 4594 Fragments arranged by size: 4594 1228 704 Acc65I G'GTAC_C Cuts at: 2095 3438 3847 2095 Size: 1343 409 4774 Fragments arranged by size: 4774 1343 409 AclI AA'CG_TT Cuts at: 1563 3474 4653 5026 1563 Size: 1911 1179 373 3063 Fragments arranged by size: 3063 1911 1179 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2312 2494 2708 3404 4589 5973 6241 1632 Size: 680 182 214 696 1185 1384 268 1917 Fragments arranged by size: 1917 1384 1185 696 680 268 214 182 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6329 AflIII A'CryG_T Cuts at: 1203 2016 5425 6402 1203 Size: 813 3409 977 1327 Fragments arranged by size: 3409 1327 977 813 AgeI A'CCGG_T Cuts at: 5357 5357 Size: 6526 AhdI GACnn_n'nnGTC Cuts at: 2116 5255 2116 Size: 3139 3387 AleI CACnn'nnGTG Cuts at: 1166 2364 1166 Size: 1198 5328 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6526 AlwNI CAG_nnn'CTG Cuts at: 2362 2414 4269 5841 2362 Size: 52 1855 1572 3047 Fragments arranged by size: 3047 1855 1572 52 ApaI G_GGCC'C Cuts at: 2237 2237 Size: 6526 ApaLI G'TGCA_C Cuts at: 4583 5739 4583 Size: 1156 5370 ApoI r'AATT_y Cuts at: 1493 3348 3835 4061 4213 6322 1493 Size: 1855 487 226 152 2109 1697 Fragments arranged by size: 2109 1855 1697 487 226 152 AseI AT'TA_AT Cuts at: 160 1032 5080 160 Size: 872 4048 1606 Fragments arranged by size: 4048 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 AvaI C'yCGr_G Cuts at: 1870 1943 2150 2168 3149 3851 1870 Size: 73 207 18 981 702 4545 Fragments arranged by size: 4545 981 702 207 73 18 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2563 2596 2563 Size: 33 6493 BamHI G'GATC_C Cuts at: 3856 4223 3856 Size: 367 6159 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2095 2700 3438 3847 5302 Size: 325 217 627 308 605 738 409 1455 Cuts at: 5302 618 Size: 1842 Fragments arranged by size: 1842 1455 738 627 605 409 325 308 217 BanII G_rGCy'C Cuts at: 1391 2237 2928 3358 3791 3845 1391 Size: 846 691 430 433 54 4072 Fragments arranged by size: 4072 846 691 433 430 54 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3552 928 Size: 971 1653 3902 Fragments arranged by size: 3902 1653 971 BbvCI CC'TCA_GC Cuts at: 2720 2763 2720 Size: 43 6483 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2021 2055 3574 3608 4717 4751 1903 Size: 34 84 34 1519 34 1109 34 3678 Fragments arranged by size: 3678 1519 1109 84 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2564 3513 4418 5628 2564 Size: 949 905 1210 3462 Fragments arranged by size: 3462 1210 949 905 BclI T'GATC_A Cuts at: 1548 2389 1548 Size: 841 5685 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5137 136 Size: 107 122 71 631 605 337 3128 1525 Fragments arranged by size: 3128 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6521 6521 Size: 6526 BlpI GC'TnA_GC Cuts at: 3117 3943 3117 Size: 826 5700 Bme1580I G_kGCm'C Cuts at: 1790 2221 2237 2703 4587 5743 1790 Size: 431 16 466 1884 1156 2573 Fragments arranged by size: 2573 1884 1156 466 431 16 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6526 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2048 3631 4257 Size: 416 53 83 399 24 631 1583 626 Cuts at: 4257 5215 442 Size: 958 1753 Fragments arranged by size: 1753 1583 958 631 626 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6526 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3713 3713 Size: 6526 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2285 2342 2654 2666 3212 3221 3288 Size: 842 204 57 312 12 546 9 67 Cuts at: 3288 3342 5186 1239 Size: 54 1844 2579 Fragments arranged by size: 2579 1844 842 546 312 204 67 57 54 12 9 Bpu10I CC'TnA_GC Cuts at: 2720 2763 2720 Size: 43 6483 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3683 4651 5516 5814 3683 Size: 968 865 298 4395 Fragments arranged by size: 4395 968 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3173 3541 5189 882 Size: 617 1674 368 1648 2219 Fragments arranged by size: 2219 1674 1648 617 368 BsaAI yAC'GTr Cuts at: 493 1204 4317 493 Size: 711 3113 2702 Fragments arranged by size: 3113 2702 711 BsaBI GATnn'nnATC Cuts at: 3240 6520 3240 Size: 3280 3246 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 4333 4715 5350 Size: 53 83 186 936 501 2299 382 635 Cuts at: 5350 275 Size: 1451 Fragments arranged by size: 2299 1451 936 635 501 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2047 3555 4959 5357 5631 5778 6189 2047 Size: 1508 1404 398 274 147 411 2384 Fragments arranged by size: 2384 1508 1404 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6301 Fragments arranged by size: 6301 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2362 2669 2747 2894 2960 3702 3751 1186 Size: 1176 307 78 147 66 742 49 3961 Fragments arranged by size: 3961 1176 742 307 147 78 66 49 BseYI C'CCAG_C Cuts at: 2139 3401 5729 6225 2139 Size: 1262 2328 496 2440 Fragments arranged by size: 2440 2328 1262 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2566 3014 1163 Size: 573 830 448 4675 Fragments arranged by size: 4675 830 573 448 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2209 2302 3976 4737 5765 1540 Size: 275 186 208 93 1674 761 1028 2301 Fragments arranged by size: 2301 1674 1028 761 275 208 186 93 BsiHKAI G_wGCw'C Cuts at: 2751 2928 3358 3616 3708 4587 4672 5743 2751 Size: 177 430 258 92 879 85 1071 3534 Fragments arranged by size: 3534 1071 879 430 258 177 92 85 BsmI GAATG_Cn' Cuts at: 4037 4130 4037 Size: 93 6433 BsmBI CGTCTCn'nnnn_ Cuts at: 2681 2681 Size: 6526 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2221 2237 2703 2751 2928 3358 3616 Size: 399 431 16 466 48 177 430 258 Cuts at: 3616 3708 3791 3845 4587 4672 5743 1391 Size: 92 83 54 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 466 431 430 399 258 177 92 85 83 54 48 16 BspEI T'CCGG_A Cuts at: 6189 6189 Size: 6526 BspHI T'CATG_A Cuts at: 4415 6145 4415 Size: 1730 4796 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2230 3881 844 Size: 1386 1651 3489 Fragments arranged by size: 3489 1651 1386 BsrBI CCG'CTC Cuts at: 1394 1823 3759 4413 1394 Size: 429 1936 654 3507 Fragments arranged by size: 3507 1936 654 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5021 5195 65 Size: 1183 3773 174 1396 Fragments arranged by size: 3773 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 2092 2311 3280 5170 5357 1700 Size: 110 256 26 219 969 1890 187 2869 Fragments arranged by size: 2869 1890 969 256 219 187 110 26 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6526 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6526 BssSI C'ACGA_G Cuts at: 3615 4586 5598 3615 Size: 971 1012 4543 Fragments arranged by size: 4543 1012 971 BstAPI GCAn_nnn'nTGC Cuts at: 794 2908 794 Size: 2114 4412 BstBI TT'CG_AA Cuts at: 5330 5330 Size: 6526 BstEII G'GTnAC_C Cuts at: 1184 2663 3283 1184 Size: 1479 620 4427 Fragments arranged by size: 4427 1479 620 BstXI CCAn_nnnn'nTGG Cuts at: 2277 2660 2277 Size: 383 6143 BstYI r'GATC_y Cuts at: 1972 3856 4223 4608 4625 6077 6163 6175 6521 Size: 1884 367 385 17 1452 86 12 346 Cuts at: 6521 1972 Size: 1977 Fragments arranged by size: 1977 1884 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2203 513 Size: 552 939 199 4836 Fragments arranged by size: 4836 939 552 199 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2743 487 Size: 683 56 1517 4270 Fragments arranged by size: 4270 1517 683 56 BtsI GCAGTG_nn' Cuts at: 2087 2270 2554 3111 4042 4836 4856 2087 Size: 183 284 557 931 794 20 3757 Fragments arranged by size: 3757 931 794 557 284 183 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6526 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6491 DraI TTT'AAA Cuts at: 3830 3830 Size: 6526 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5533 809 Size: 507 4217 1802 Fragments arranged by size: 4217 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3013 3973 4862 Size: 54 1425 50 275 18 1183 960 889 Cuts at: 4862 6491 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1183 960 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3973 1537 Size: 275 2161 4090 Fragments arranged by size: 4090 2161 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2513 3063 3423 4456 1353 Size: 1160 550 360 1033 3423 Fragments arranged by size: 3423 1160 1033 550 360 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3725 5113 5487 5633 387 Size: 1064 138 2136 1388 374 146 1280 Fragments arranged by size: 2136 1388 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2926 3356 2926 Size: 430 6096 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2285 2312 2342 2494 2654 2666 Size: 393 449 204 27 30 152 160 12 Cuts at: 2666 2708 3212 3221 3288 3342 3404 4589 5186 Size: 42 504 9 67 54 62 1185 597 Cuts at: 5186 5973 6241 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1185 787 597 504 449 393 268 204 160 152 67 62 54 42 30 27 12 9 EcoO109I rG'GnC_Cy Cuts at: 2158 2165 2158 Size: 7 6519 EcoRI G'AATT_C Cuts at: 3348 3835 4213 3348 Size: 487 378 5661 Fragments arranged by size: 5661 487 378 FseI GG_CCGG'CC Cuts at: 2070 2070 Size: 6526 FspI TGC'GCA Cuts at: 5032 5032 Size: 6526 HaeII r_GCGC'y Cuts at: 1678 5673 1678 Size: 3995 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 3062 3094 5167 Size: 32 135 32 530 32 928 32 2073 Cuts at: 5167 5199 5241 5273 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2073 928 530 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2317 3145 3870 4116 669 Size: 988 281 379 828 725 246 3079 Fragments arranged by size: 3079 988 828 725 379 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6526 HpaI GTT'AAC Cuts at: 4116 4116 Size: 6526 KpnI G_GTAC'C Cuts at: 2099 3442 3851 2099 Size: 1343 409 4774 Fragments arranged by size: 4774 1343 409 MfeI C'AATT_G Cuts at: 2906 4125 2906 Size: 1219 5307 MluI A'CGCG_T Cuts at: 6402 6402 Size: 6526 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3519 3741 5640 5824 1305 Size: 2214 222 1899 184 2007 Fragments arranged by size: 2214 2007 1899 222 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3015 10 Size: 54 1425 343 1183 3521 Fragments arranged by size: 3521 1425 1183 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2364 3639 4484 4843 5002 518 Size: 648 1198 1275 845 359 159 2042 Fragments arranged by size: 2042 1275 1198 845 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2101 2205 2660 2686 2959 3199 3794 3942 Size: 495 104 455 26 273 240 595 148 Cuts at: 3942 4619 5767 6012 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 595 495 455 273 245 240 148 104 26 NaeI GCC'GGC Cuts at: 2068 2068 Size: 6526 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5035 Fragments arranged by size: 5035 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6526 NgoMIV G'CCGG_C Cuts at: 2066 2066 Size: 6526 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6526 NotI GC'GGCC_GC Cuts at: 3973 3973 Size: 6526 NspI r_CATG'y Cuts at: 1132 1229 2588 3884 5429 1132 Size: 97 1359 1296 1545 2229 Fragments arranged by size: 2229 1545 1359 1296 97 PasI CC'CwG_GG Cuts at: 1348 2696 1348 Size: 1348 5178 PciI A'CATG_T Cuts at: 5425 5425 Size: 6526 PflMI CCAn_nnn'nTGG Cuts at: 1108 2362 1108 Size: 1254 5272 PmeI GTTT'AAAC Cuts at: 3830 3830 Size: 6526 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4595 4627 6108 6140 4595 Size: 32 1481 32 4981 Fragments arranged by size: 4981 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2158 2165 2158 Size: 7 6519 PshAI GACnn'nnGTC Cuts at: 1613 2163 1613 Size: 550 5976 PsiI TTA'TAA Cuts at: 4096 4096 Size: 6526 PspOMI G'GGCC_C Cuts at: 2233 2233 Size: 6526 PspXI vC'TCGA_Gb Cuts at: 3149 3149 Size: 6526 PstI C_TGCA'G Cuts at: 830 2184 2922 2958 3078 3878 830 Size: 1354 738 36 120 800 3478 Fragments arranged by size: 3478 1354 800 738 120 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6526 PvuII CAG'CTG Cuts at: 2660 2686 2959 3199 2660 Size: 26 273 240 5987 Fragments arranged by size: 5987 273 240 26 SacI G_AGCT'C Cuts at: 2928 3358 2928 Size: 430 6096 SacII CC_GC'GG Cuts at: 2206 2206 Size: 6526 SalI G'TCGA_C Cuts at: 1936 3868 1936 Size: 1932 4594 SapI GCTCTTCn'nnn_ Cuts at: 3063 3063 Size: 6526 SbfI CC_TGCA'GG Cuts at: 3878 3878 Size: 6526 ScaI AGT'ACT Cuts at: 4774 6486 4774 Size: 1712 4814 SfcI C'TryA_G Cuts at: 826 1045 2180 2347 2918 2954 3074 3874 5009 Size: 219 1135 167 571 36 120 800 1135 Cuts at: 5009 5690 5881 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1135 800 681 571 219 191 167 120 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6526 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6526 SmaI CCC'GGG Cuts at: 1872 2152 3853 1872 Size: 280 1701 4545 Fragments arranged by size: 4545 1701 280 SmlI C'TyrA_G Cuts at: 820 1017 1943 3149 3698 4630 5531 5793 820 Size: 197 926 1206 549 932 901 262 1553 Fragments arranged by size: 1553 1206 932 926 901 549 262 197 SnaBI TAC'GTA Cuts at: 493 4317 493 Size: 3824 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6526 SphI G_CATG'C Cuts at: 1132 3884 1132 Size: 2752 3774 SspI AAT'ATT Cuts at: 5 52 4450 5 Size: 47 4398 2081 Fragments arranged by size: 4398 2081 47 StuI AGG'CCT Cuts at: 1517 3752 1517 Size: 2235 4291 StyI C'CwwG_G Cuts at: 513 1065 2004 2481 3965 513 Size: 552 939 477 1484 3074 Fragments arranged by size: 3074 1484 939 552 477 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 2994 4553 4570 4723 4908 5353 Size: 1255 774 640 1559 17 153 185 445 Cuts at: 5353 325 Size: 1498 Fragments arranged by size: 1559 1498 1255 774 640 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3423 4772 6484 Size: 274 80 33 51 1443 1446 1349 1712 Cuts at: 6484 96 Size: 138 Fragments arranged by size: 1712 1446 1443 1349 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3487 3519 3698 3730 6486 6518 3487 Size: 32 179 32 2756 32 3495 Fragments arranged by size: 3495 2756 179 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6526 XbaI T'CTAG_A Cuts at: 3004 3862 3004 Size: 858 5668 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2132 2854 2132 Size: 722 5804 XhoI C'TCGA_G Cuts at: 1943 3149 1943 Size: 1206 5320 XmaI C'CCGG_G Cuts at: 1870 2150 3851 1870 Size: 280 1701 4545 Fragments arranged by size: 4545 1701 280 XmnI GAAnn'nnTTC Cuts at: 2018 3348 3476 4655 2018 Size: 1330 128 1179 3889 Fragments arranged by size: 3889 1330 1179 128 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4334 276 Size: 53 83 186 936 2800 2468 Fragments arranged by size: 2800 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI