(Linear) (Six-base) MAPSORT of: KIEE4162.seq Check: 1194 from: 1 to: 1821 KIEE4162.orf With 182 enzymes: SgfI * March 22, 2012 17:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 225 1821 Size: 225 1596 AccI GT'mk_AC Cuts at: 0 1160 1821 Size: 1160 661 Acc65I G'GTAC_C Cuts at: 0 90 1433 1821 Size: 90 1343 388 Fragments arranged by size: 1343 388 90 AclI AA'CG_TT Cuts at: 0 1469 1821 Size: 1469 352 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 307 489 703 1399 1821 Size: 307 182 214 696 422 Fragments arranged by size: 696 422 307 214 182 AflIII A'CryG_T Cuts at: 0 11 1821 Size: 11 1810 AhdI GACnn_n'nnGTC Cuts at: 0 111 1821 Size: 111 1710 AleI CACnn'nnGTG Cuts at: 0 359 1821 Size: 359 1462 AlwNI CAG_nnn'CTG Cuts at: 0 357 409 1821 Size: 357 52 1412 Fragments arranged by size: 1412 357 52 ApaI G_GGCC'C Cuts at: 0 232 1821 Size: 232 1589 ApoI r'AATT_y Cuts at: 0 1343 1821 Size: 1343 478 AvaI C'yCGr_G Cuts at: 0 145 163 1144 1821 Size: 145 18 981 677 Fragments arranged by size: 981 677 145 18 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 558 591 1821 Size: 558 33 1230 Fragments arranged by size: 1230 558 33 BanI G'GyrC_C Cuts at: 0 90 695 1433 1821 Size: 90 605 738 388 Fragments arranged by size: 738 605 388 90 BanII G_rGCy'C Cuts at: 0 232 923 1353 1786 1821 Size: 232 691 430 433 35 Fragments arranged by size: 691 433 430 232 35 BbsI GAAGACnn'nnnn_ Cuts at: 0 1547 1821 Size: 1547 274 BbvCI CC'TCA_GC Cuts at: 0 715 758 1821 Size: 715 43 1063 Fragments arranged by size: 1063 715 43 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 16 50 1569 1603 1821 Size: 16 34 1519 34 218 Fragments arranged by size: 1519 218 34 34 16 BciVI GTATCCnnnnn_n' Cuts at: 0 559 1508 1821 Size: 559 949 313 Fragments arranged by size: 949 559 313 BclI T'GATC_A Cuts at: 0 384 1821 Size: 384 1437 BlpI GC'TnA_GC Cuts at: 0 1112 1821 Size: 1112 709 Bme1580I G_kGCm'C Cuts at: 0 216 232 698 1821 Size: 216 16 466 1123 Fragments arranged by size: 1123 466 216 16 BmrI ACTGGGnnnn_n' Cuts at: 0 43 1626 1821 Size: 43 1583 195 Fragments arranged by size: 1583 195 43 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1708 1821 Size: 1708 113 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 280 337 649 661 1207 1216 1283 Size: 76 204 57 312 12 546 9 67 Cuts at: 1283 1337 1821 Size: 54 484 Fragments arranged by size: 546 484 312 204 76 67 57 54 12 9 Bpu10I CC'TnA_GC Cuts at: 0 715 758 1821 Size: 715 43 1063 Fragments arranged by size: 1063 715 43 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1678 1821 Size: 1678 143 BsaI GGTCTCn'nnnn_ Cuts at: 0 1168 1536 1821 Size: 1168 368 285 Fragments arranged by size: 1168 368 285 BsaBI GATnn'nnATC Cuts at: 0 1235 1821 Size: 1235 586 BsaHI Gr'CG_yC Cuts at: 0 29 1821 Size: 29 1792 BsaWI w'CCGG_w Cuts at: 0 42 1550 1821 Size: 42 1508 271 Fragments arranged by size: 1508 271 42 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 357 664 742 889 955 1697 1746 1821 Size: 357 307 78 147 66 742 49 75 Fragments arranged by size: 742 357 307 147 78 75 66 49 BseYI C'CCAG_C Cuts at: 0 134 1396 1821 Size: 134 1262 425 Fragments arranged by size: 1262 425 134 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 561 1009 1821 Size: 561 448 812 Fragments arranged by size: 812 561 448 BsiEI CG_ry'CG Cuts at: 0 204 297 1821 Size: 204 93 1524 Fragments arranged by size: 1524 204 93 BsiHKAI G_wGCw'C Cuts at: 0 746 923 1353 1611 1703 1821 Size: 746 177 430 258 92 118 Fragments arranged by size: 746 430 258 177 118 92 BsmBI CGTCTCn'nnnn_ Cuts at: 0 676 1821 Size: 676 1145 Bsp1286I G_dGCh'C Cuts at: 0 216 232 698 746 923 1353 1611 1703 Size: 216 16 466 48 177 430 258 92 Cuts at: 1703 1786 1821 Size: 83 35 Fragments arranged by size: 466 430 258 216 177 92 83 48 35 16 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 225 1821 Size: 225 1596 BsrBI CCG'CTC Cuts at: 0 1754 1821 Size: 1754 67 BsrFI r'CCGG_y Cuts at: 0 61 87 306 1275 1821 Size: 61 26 219 969 546 Fragments arranged by size: 969 546 219 61 26 BssSI C'ACGA_G Cuts at: 0 1610 1821 Size: 1610 211 BstAPI GCAn_nnn'nTGC Cuts at: 0 903 1821 Size: 903 918 BstEII G'GTnAC_C Cuts at: 0 658 1278 1821 Size: 658 620 543 Fragments arranged by size: 658 620 543 BstXI CCAn_nnnn'nTGG Cuts at: 0 272 655 1821 Size: 272 383 1166 Fragments arranged by size: 1166 383 272 BtgI C'CryG_G Cuts at: 0 198 1821 Size: 198 1623 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 738 1821 Size: 738 1083 BtsI GCAGTG_nn' Cuts at: 0 82 265 549 1106 1821 Size: 82 183 284 557 715 Fragments arranged by size: 715 557 284 183 82 EaeI y'GGCC_r Cuts at: 0 1008 1821 Size: 1008 813 EarI CTCTTCn'nnn_ Cuts at: 0 508 1058 1418 1821 Size: 508 550 360 403 Fragments arranged by size: 550 508 403 360 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1720 1821 Size: 1720 101 EcoICRI GAG'CTC Cuts at: 0 921 1351 1821 Size: 921 430 470 Fragments arranged by size: 921 470 430 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 280 307 337 489 649 661 703 Size: 76 204 27 30 152 160 12 42 Cuts at: 703 1207 1216 1283 1337 1399 1821 Size: 504 9 67 54 62 422 Fragments arranged by size: 504 422 204 160 152 76 67 62 54 42 30 27 12 9 EcoO109I rG'GnC_Cy Cuts at: 0 153 160 1821 Size: 153 7 1661 Fragments arranged by size: 1661 153 7 EcoRI G'AATT_C Cuts at: 0 1343 1821 Size: 1343 478 FseI GG_CCGG'CC Cuts at: 0 65 1821 Size: 65 1756 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 97 129 1057 1089 1821 Size: 97 32 928 32 732 Fragments arranged by size: 928 732 97 32 32 HincII GTy'rAC Cuts at: 0 312 1140 1821 Size: 312 828 681 Fragments arranged by size: 828 681 312 KpnI G_GTAC'C Cuts at: 0 94 1437 1821 Size: 94 1343 384 Fragments arranged by size: 1343 384 94 MfeI C'AATT_G Cuts at: 0 901 1821 Size: 901 920 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1514 1736 1821 Size: 1514 222 85 Fragments arranged by size: 1514 222 85 MscI TGG'CCA Cuts at: 0 1010 1821 Size: 1010 811 MslI CAynn'nnrTG Cuts at: 0 359 1634 1821 Size: 359 1275 187 Fragments arranged by size: 1275 359 187 MspA1I CmG'CkG Cuts at: 0 96 200 655 681 954 1194 1789 1821 Size: 96 104 455 26 273 240 595 32 Fragments arranged by size: 595 455 273 240 104 96 32 26 NaeI GCC'GGC Cuts at: 0 63 1821 Size: 63 1758 NgoMIV G'CCGG_C Cuts at: 0 61 1821 Size: 61 1760 NspI r_CATG'y Cuts at: 0 583 1821 Size: 583 1238 PasI CC'CwG_GG Cuts at: 0 691 1821 Size: 691 1130 PflMI CCAn_nnn'nTGG Cuts at: 0 357 1821 Size: 357 1464 PpuMI rG'GwC_Cy Cuts at: 0 153 160 1821 Size: 153 7 1661 Fragments arranged by size: 1661 153 7 PshAI GACnn'nnGTC Cuts at: 0 158 1821 Size: 158 1663 PspOMI G'GGCC_C Cuts at: 0 228 1821 Size: 228 1593 PspXI vC'TCGA_Gb Cuts at: 0 1144 1821 Size: 1144 677 PstI C_TGCA'G Cuts at: 0 179 917 953 1073 1821 Size: 179 738 36 120 748 Fragments arranged by size: 748 738 179 120 36 PvuII CAG'CTG Cuts at: 0 655 681 954 1194 1821 Size: 655 26 273 240 627 Fragments arranged by size: 655 627 273 240 26 SacI G_AGCT'C Cuts at: 0 923 1353 1821 Size: 923 430 468 Fragments arranged by size: 923 468 430 SacII CC_GC'GG Cuts at: 0 201 1821 Size: 201 1620 SapI GCTCTTCn'nnn_ Cuts at: 0 1058 1821 Size: 1058 763 SfcI C'TryA_G Cuts at: 0 175 342 913 949 1069 1821 Size: 175 167 571 36 120 752 Fragments arranged by size: 752 571 175 167 120 36 SmaI CCC'GGG Cuts at: 0 147 1821 Size: 147 1674 SmlI C'TyrA_G Cuts at: 0 1144 1693 1821 Size: 1144 549 128 Fragments arranged by size: 1144 549 128 StuI AGG'CCT Cuts at: 0 1747 1821 Size: 1747 74 StyI C'CwwG_G Cuts at: 0 476 1821 Size: 476 1345 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 349 989 1821 Size: 349 640 832 Fragments arranged by size: 832 640 349 TatI w'GTAC_w Cuts at: 0 1418 1821 Size: 1418 403 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1482 1514 1693 1725 1821 Size: 1482 32 179 32 96 Fragments arranged by size: 1482 179 96 32 32 XbaI T'CTAG_A Cuts at: 0 999 1821 Size: 999 822 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 127 849 1821 Size: 127 722 972 Fragments arranged by size: 972 722 127 XhoI C'TCGA_G Cuts at: 0 1144 1821 Size: 1144 677 XmaI C'CCGG_G Cuts at: 0 145 1821 Size: 145 1676 XmnI GAAnn'nnTTC Cuts at: 0 13 1343 1471 1821 Size: 13 1330 128 350 Fragments arranged by size: 1330 350 128 13 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AhdI AleI AlwNI ApaI ApoI AvaI BaeI BanI BanII BbsI BbvCI BcgI BciVI BclI BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstEII BstXI BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI Hin4I HincII KpnI MfeI MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI PflMI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII SapI SfcI SmaI SmlI StuI StyI TaqII TatI TstI XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AgeI AlfI AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BfrBI BglI BglII BmgBI BmtI BsaAI BsaXI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRV FalI FspI FspAI HaeII HindIII HpaI KasI MluI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PsiI PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I ZraI