Restriction map for
pFN21AE9616
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CfoI ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRV EcoT22I
FauI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI KpnI Ksp632I KspI
MluI MluNI MroI MscI MslI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 155 GAATTC [GA]^AATT[CT]
AocI 456 CCTAAGG CC^T[ACGT]AGG
ApoI 155 GAATTC [GA]^AATT[CT]
AspHI 355 GAGCTC G[AT]GC[AT]^C
AvaII 40 GGACC G^G[AT]CC
BanII 355 GAGCTC G[GA]GC[CT]^C
BbsI 783 GTCTTC GAAGAC(2/6)
BclI 63 TGATCA T^GATCA
BlpI 357 GCTCAGC GC^T[ACGT]AGC
BmyI 355 GAGCTC G[AGT]GC[ACT]^C
BpmI 124 CTGGAG CTGGAG(16/14)
Bpu1102I 357 GCTCAGC GC^T[ACGT]AGC
BpuAI 783 GTCTTC GAAGAC(2/6)
BseRI 242 CTCCTC GAGGAG(10/8)
BsiHKAI 355 GAGCTC G[AT]GC[AT]^C
Bsp1286I 355 GAGCTC G[AGT]GC[ACT]^C
BspHI 60 TCATGA T^CATGA
BspMI 1032 ACCTGC ACCTGC(4/8)
Bsu36I 456 CCTAAGG CC^T[ACGT]AGG
CelII 357 GCTCAGC GC^T[ACGT]AGC
Cfr13I 40 GGACC G^G[ACGT]CC
Eco81I 456 CCTAAGG CC^T[ACGT]AGG
EcoNI 175 CCTAAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 155 GAATTC G^AATTC
EcoT14I 162 CCTTGG C^C[AT][AT]GG
FbaI 63 TGATCA T^GATCA
HindIII 1012 AAGCTT A^AGCTT
MaeII 663 ACGT A^CGT
MaeIII 10 GTGAC ^GT[ACGT]AC
MfeI 70 CAATTG C^AATTG
MseI 803 TTAA T^TAA
MspA1I 901 CAGCTG C[AC]G^C[GT]G
MunI 70 CAATTG C^AATTG
NlaIV 762 GGAACC GG[ACGT]^[ACGT]CC
NspI 971 GCATGC [GA]CATG^[CT]
PleI 57 GACTC GAGTC(4/5)
PshAI 215 GACTTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 904 CTGCAG CTGCA^G
PvuII 901 CAGCTG CAG^CTG
RcaI 60 TCATGA T^CATGA
SacI 355 GAGCTC GAGCT^C
Sau96I 40 GGACC G^G[ACGT]CC
SmlI 245 CTCAAG C^T[CT][GA]AG
SphI 971 GCATGC GCATG^C
SspI 828 AATATT AAT^ATT
StuI 908 AGGCCT AGG^CCT
StyI 162 CCTTGG C^C[AT][AT]GG
Tru9I 803 TTAA T^TAA
Tsp45I 10 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 288 GCGG CCGC(-3/-1)
318 GCGG
AfaI 926 GTAC GT^AC
952 GTAC
Asp700I 151 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
211 GAAAGACTTC
BbvI 327 GCTGC GCAGC(8/12)
903 GCTGC
BfaI 14 CTAG C^TAG
170 CTAG
BsaBI 1046 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1052 GATCTGGATC
BsiYI 175 CCTAAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
696 CCAGCAGGAGG
BslI 175 CCTAAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
696 CCAGCAGGAGG
BsmFI 461 GGGAC GGGAC(10/14)
228 GTCCC
BsrFI 314 ACCGGC [GA]^CCGG[CT]
362 GCCGGT
Cfr10I 314 ACCGGC [GA]^CCGG[CT]
362 GCCGGT
HaeIII 738 GGCC GG^CC
909 GGCC
HapII 315 CCGG C^CGG
363 CCGG
HpaII 315 CCGG C^CGG
363 CCGG
MaeI 14 CTAG C^TAG
170 CTAG
MamI 1046 GATCTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1052 GATCTGGATC
MspI 315 CCGG C^CGG
363 CCGG
RsaI 926 GTAC GT^AC
952 GTAC
SfcI 904 CTGCAG C^T[GA][CT]AG
1021 CTACAG
TseI 327 GCTGC G^C[AT]GC
903 GCTGC
XmnI 151 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
211 GAAAGACTTC
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# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI BsaJI BsmAI BsrI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII EcoRII Fnu4HI FokI
HinfI HphI ItaI MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIII Sau3AI ScrFI SfaNI TaqI
TfiI Tsp509I TspRI TthHB8I XhoII