(Circular) (Six-base) MAPSORT of: pFN21AE9616.seq Check: 1634 from: 1 to: 5773 pFN21AE9616 5773 bp With 182 enzymes: SgfI * March 28, 2012 12:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3046 3046 Size: 5773 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3583 278 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3116 1937 Size: 1179 4594 Acc65I G'GTAC_C Cuts at: 3094 3094 Size: 5773 AclI AA'CG_TT Cuts at: 1563 3900 4273 1563 Size: 2337 373 3063 Fragments arranged by size: 3063 2337 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1995 2207 2464 2541 3005 3836 5220 5488 Size: 363 212 257 77 464 831 1384 268 Cuts at: 5488 1632 Size: 1917 Fragments arranged by size: 1917 1384 831 464 363 268 257 212 77 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5576 AflIII A'CryG_T Cuts at: 1203 4672 5649 1203 Size: 3469 977 1327 Fragments arranged by size: 3469 1327 977 AgeI A'CCGG_T Cuts at: 4604 4604 Size: 5773 AhdI GACnn_n'nnGTC Cuts at: 4502 4502 Size: 5773 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5773 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5773 AlwNI CAG_nnn'CTG Cuts at: 3516 5088 3516 Size: 1572 4201 ApaLI G'TGCA_C Cuts at: 3830 4986 3830 Size: 1156 4617 ApoI r'AATT_y Cuts at: 1493 2160 3082 3308 3460 5569 1493 Size: 667 922 226 152 2109 1697 Fragments arranged by size: 2109 1697 922 667 226 152 AseI AT'TA_AT Cuts at: 160 1032 4327 160 Size: 872 3295 1606 Fragments arranged by size: 3295 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 AvaI C'yCGr_G Cuts at: 1870 1943 3098 1870 Size: 73 1155 4545 Fragments arranged by size: 4545 1155 73 BamHI G'GATC_C Cuts at: 3103 3470 3103 Size: 367 5406 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3094 4549 618 Size: 325 217 627 1307 1455 1842 Fragments arranged by size: 1842 1455 1307 627 325 217 BanII G_rGCy'C Cuts at: 1391 2364 3092 1391 Size: 973 728 4072 Fragments arranged by size: 4072 973 728 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2781 928 Size: 971 882 3920 Fragments arranged by size: 3920 971 882 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3964 3998 1903 Size: 34 2027 34 3678 Fragments arranged by size: 3678 2027 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3665 4875 3665 Size: 1210 4563 BclI T'GATC_A Cuts at: 1548 2068 1548 Size: 520 5253 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4384 136 Size: 107 122 71 631 605 2712 1525 Fragments arranged by size: 2712 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5768 5768 Size: 5773 BlpI GC'TnA_GC Cuts at: 2363 3190 2363 Size: 827 4946 Bme1580I G_kGCm'C Cuts at: 1790 3834 4990 1790 Size: 2044 1156 2573 Fragments arranged by size: 2573 2044 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2929 3504 4462 Size: 416 53 83 399 24 1512 575 958 Cuts at: 4462 442 Size: 1753 Fragments arranged by size: 1753 1512 958 575 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5773 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2150 4433 1239 Size: 911 2283 2579 Fragments arranged by size: 2579 2283 911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2235 3898 4763 5061 2235 Size: 1663 865 298 2947 Fragments arranged by size: 2947 1663 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4436 882 Size: 617 2937 2219 Fragments arranged by size: 2937 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3564 493 Size: 711 2360 2702 Fragments arranged by size: 2702 2360 711 BsaBI GATnn'nnATC Cuts at: 3055 3061 5767 3055 Size: 6 2706 3061 Fragments arranged by size: 3061 2706 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3580 3962 4597 275 Size: 53 83 186 936 2047 382 635 1451 Fragments arranged by size: 2047 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4206 4604 4878 5025 5436 4206 Size: 398 274 147 411 4543 Fragments arranged by size: 4543 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5548 Fragments arranged by size: 5548 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2238 1186 Size: 1052 4721 BseYI C'CCAG_C Cuts at: 4976 5472 4976 Size: 496 5277 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5200 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3223 3984 5012 1540 Size: 275 186 1222 761 1028 2301 Fragments arranged by size: 2301 1222 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2364 3834 3919 4990 2364 Size: 1470 85 1071 3147 Fragments arranged by size: 3147 1470 1071 85 BsmI GAATG_Cn' Cuts at: 3284 3377 3284 Size: 93 5680 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2364 3092 3834 3919 4990 1391 Size: 399 574 728 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 728 574 399 85 BspEI T'CCGG_A Cuts at: 5436 5436 Size: 5773 BspHI T'CATG_A Cuts at: 2065 3662 5392 2065 Size: 1597 1730 2446 Fragments arranged by size: 2446 1730 1597 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3046 3128 844 Size: 2202 82 3489 Fragments arranged by size: 3489 2202 82 BsrBI CCG'CTC Cuts at: 1394 1823 3660 1394 Size: 429 1837 3507 Fragments arranged by size: 3507 1837 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4268 4442 65 Size: 1183 3020 174 1396 Fragments arranged by size: 3020 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2319 2367 4417 4604 1700 Size: 110 509 48 2050 187 2869 Fragments arranged by size: 2869 2050 509 187 110 48 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5773 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5773 BssSI C'ACGA_G Cuts at: 3833 4845 3833 Size: 1012 4761 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5773 BstBI TT'CG_AA Cuts at: 4577 4577 Size: 5773 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5773 BstYI r'GATC_y Cuts at: 1972 2989 3050 3056 3062 3103 3470 3855 3872 Size: 1017 61 6 6 41 367 385 17 Cuts at: 3872 5324 5410 5422 5768 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1017 385 367 346 86 61 41 17 12 6 6 Bsu36I CC'TnA_GG Cuts at: 2462 2462 Size: 5773 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5034 Fragments arranged by size: 5034 683 56 BtsI GCAGTG_nn' Cuts at: 3289 4083 4103 3289 Size: 794 20 4959 Fragments arranged by size: 4959 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5738 DraI TTT'AAA Cuts at: 3077 3077 Size: 5773 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4780 809 Size: 507 3464 1802 Fragments arranged by size: 3464 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3220 4109 5738 Size: 54 1425 50 275 18 1390 889 1629 Cuts at: 5738 8 Size: 43 Fragments arranged by size: 1629 1425 1390 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3220 1537 Size: 275 1408 4090 Fragments arranged by size: 4090 1408 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3703 1353 Size: 2350 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2338 4360 4734 4880 387 Size: 1064 138 749 2022 374 146 1280 Fragments arranged by size: 2022 1280 1064 749 374 146 138 EcoICRI GAG'CTC Cuts at: 2362 2362 Size: 5773 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1995 2150 2207 2464 2541 3005 3836 Size: 393 363 155 57 257 77 464 831 Cuts at: 3836 4433 5220 5488 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 831 787 597 464 393 363 268 257 155 77 57 EcoNI CCTnn'n_nnAGG Cuts at: 2184 2184 Size: 5773 EcoRI G'AATT_C Cuts at: 2160 3082 3460 2160 Size: 922 378 4473 Fragments arranged by size: 4473 922 378 FspI TGC'GCA Cuts at: 4279 4279 Size: 5773 HaeII r_GCGC'y Cuts at: 1678 4920 1678 Size: 3242 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2013 2045 2053 2085 2665 Size: 32 135 32 441 32 8 32 580 Cuts at: 2665 2697 2708 2740 4414 4446 4488 4520 1373 Size: 32 11 32 1674 32 42 32 2626 Fragments arranged by size: 2626 1674 580 441 135 42 32 32 32 32 32 32 32 32 11 8 HincII GTy'rAC Cuts at: 669 1657 1938 3117 3363 669 Size: 988 281 1179 246 3079 Fragments arranged by size: 3079 1179 988 281 246 HindIII A'AGCT_T Cuts at: 748 3017 748 Size: 2269 3504 HpaI GTT'AAC Cuts at: 3363 3363 Size: 5773 KpnI G_GTAC'C Cuts at: 3098 3098 Size: 5773 MfeI C'AATT_G Cuts at: 2075 3372 2075 Size: 1297 4476 MluI A'CGCG_T Cuts at: 5649 5649 Size: 5773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4887 5071 1305 Size: 3582 184 2007 Fragments arranged by size: 3582 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3951 Fragments arranged by size: 3951 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3731 4090 4249 518 Size: 648 2565 359 159 2042 Fragments arranged by size: 2565 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2908 3189 3866 5014 5259 1606 Size: 1302 281 677 1148 245 2120 Fragments arranged by size: 2120 1302 1148 677 281 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5221 NdeI CA'TA_TG Cuts at: 387 387 Size: 5773 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5773 NotI GC'GGCC_GC Cuts at: 3220 3220 Size: 5773 NspI r_CATG'y Cuts at: 1132 1229 2980 3131 4676 1132 Size: 97 1751 151 1545 2229 Fragments arranged by size: 2229 1751 1545 151 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5773 PciI A'CATG_T Cuts at: 4672 4672 Size: 5773 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5773 PmeI GTTT'AAAC Cuts at: 3077 3077 Size: 5773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3842 3874 5355 5387 3842 Size: 32 1481 32 4228 Fragments arranged by size: 4228 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2224 1613 Size: 611 5162 PsiI TTA'TAA Cuts at: 3343 3343 Size: 5773 PstI C_TGCA'G Cuts at: 830 2913 3125 830 Size: 2083 212 3478 Fragments arranged by size: 3478 2083 212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5773 PvuII CAG'CTG Cuts at: 2908 2908 Size: 5773 SacI G_AGCT'C Cuts at: 2364 2364 Size: 5773 SalI G'TCGA_C Cuts at: 1936 3115 1936 Size: 1179 4594 SbfI CC_TGCA'GG Cuts at: 3125 3125 Size: 5773 ScaI AGT'ACT Cuts at: 4021 5733 4021 Size: 1712 4061 SfcI C'TryA_G Cuts at: 826 1045 2909 3026 3121 4256 4937 5128 826 Size: 219 1864 117 95 1135 681 191 1471 Fragments arranged by size: 1864 1471 1135 681 219 191 117 95 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5773 SmaI CCC'GGG Cuts at: 1872 3100 1872 Size: 1228 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2250 3877 4778 5040 820 Size: 197 926 307 1627 901 262 1553 Fragments arranged by size: 1627 1553 926 901 307 262 197 SnaBI TAC'GTA Cuts at: 493 3564 493 Size: 3071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5773 SphI G_CATG'C Cuts at: 1132 2980 3131 1132 Size: 1848 151 3774 Fragments arranged by size: 3774 1848 151 SspI AAT'ATT Cuts at: 5 52 2835 3697 5 Size: 47 2783 862 2081 Fragments arranged by size: 2783 2081 862 47 StuI AGG'CCT Cuts at: 1517 2915 1517 Size: 1398 4375 StyI C'CwwG_G Cuts at: 513 1065 2167 3212 513 Size: 552 1102 1045 3074 Fragments arranged by size: 3074 1102 1045 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3800 3817 3970 4155 4600 325 Size: 1255 2220 17 153 185 445 1498 Fragments arranged by size: 2220 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2956 4019 5731 Size: 274 80 33 51 1443 979 1063 1712 Cuts at: 5731 96 Size: 138 Fragments arranged by size: 1712 1443 1063 979 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5733 5765 5733 Size: 32 5741 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5773 XbaI T'CTAG_A Cuts at: 3109 3109 Size: 5773 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5773 XmaI C'CCGG_G Cuts at: 1870 3098 1870 Size: 1228 4545 XmnI GAAnn'nnTTC Cuts at: 2160 2220 3902 2160 Size: 60 1682 4031 Fragments arranged by size: 4031 1682 60 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3581 276 Size: 53 83 186 936 2047 2468 Fragments arranged by size: 2468 2047 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI