Restriction map for
pFN21AE1966
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BclI
BcnI BfrI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BsrFI BsrGI BssHII BssKI
BssSI Bst1107I BstBI BstEII BstNI
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRII EcoRV FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI HphI
KasI KpnI KspI MamI MluI
MvaI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI ScrFI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 1565 GTAGAC GT^[AC][GT]AC
AccIII 298 TCCGGA T^CCGGA
AflIII 1271 ACATGT A^C[GA][CT]GT
AlwNI 1549 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 439 CCTGAGG CC^T[ACGT]AGG
BalI 642 TGGCCA TGG^CCA
BglI 910 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 298 TCCGGA [AT]^CCGG[AT]
BseAI 298 TCCGGA T^CCGGA
BseRI 1355 GAGGAG GAGGAG(10/8)
BsiYI 292 CCTTACTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 292 CCTTACTCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 176 GAGAC GTCTC(1/5)
BspEI 298 TCCGGA T^CCGGA
BspHI 132 TCATGA T^CATGA
BspLU11I 1271 ACATGT A^CATGT
BspMI 1460 GCAGGT ACCTGC(4/8)
BsrBI 246 CCGCTC CCGCTC(-3/-3)
BstYI 803 GGATCT [GA]^GATC[CT]
Bsu36I 439 CCTGAGG CC^T[ACGT]AGG
Eco81I 439 CCTGAGG CC^T[ACGT]AGG
EcoRI 488 GAATTC G^AATTC
EcoT22I 1448 ATGCAT ATGCA^T
HapII 299 CCGG C^CGG
HincII 1375 GTTGAC GT[CT]^[GA]AC
HindII 1375 GTTGAC GT[CT]^[GA]AC
HindIII 1205 AAGCTT A^AGCTT
HpaII 299 CCGG C^CGG
MaeIII 976 GTTAC ^GT[ACGT]AC
MfeI 1421 CAATTG C^AATTG
MflI 803 GGATCT [GA]^GATC[CT]
MluNI 642 TGGCCA TGG^CCA
MroI 298 TCCGGA T^CCGGA
MscI 642 TGGCCA TGG^CCA
MslI 1366 CATATTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 299 CCGG C^CGG
MunI 1421 CAATTG C^AATTG
NdeI 1493 CATATG CA^TATG
NsiI 1448 ATGCAT ATGCA^T
NspI 1271 ACATGT [GA]CATG^[CT]
PleI 898 GACTC GAGTC(4/5)
RcaI 132 TCATGA T^CATGA
XcmI 911 CCAAAAAGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 803 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
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AlwI 866 GATCC GGATC(4/5)
803 GGATC
AvaII 301 GGACC G^G[AT]CC
742 GGACC
BanII 405 GAGCCC G[GA]GC[CT]^C
762 GGGCTC
BciVI 1308 GGATAC GTATCC(6/5)
833 GTATCC
Cfr13I 301 GGACC G^G[ACGT]CC
742 GGACC
EaeI 78 CGGCCA [CT]^GGCC[GA]
642 TGGCCA
EarI 1502 CTCTTC CTCTTC(1/4)
508 GAAGAG
EcoT14I 496 CCAAGG C^C[AT][AT]GG
869 CCAAGG
Ksp632I 1502 CTCTTC CTCTTC(1/4)
508 GAAGAG
Sau96I 301 GGACC G^G[ACGT]CC
742 GGACC
SmlI 31 CTTGAG C^T[CT][GA]AG
249 CTCAAG
StyI 496 CCAAGG C^C[AT][AT]GG
869 CCAAGG
TfiI 1110 GATTC G^A[AT]TC
1531 GATTC
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbsI BbvI BfaI BmyI BpuAI
BsaJI Bsp1286I BsrDI BsrI BstAPI
BstF5I Cac8I DdeI DpnII Fnu4HI
FokI HaeIII HinfI ItaI MaeI
MaeII MboI MboII MnlI MseI
MspA1I MwoI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI SfaNI
SfcI TaqI Tru9I TseI Tsp509I
TspRI TthHB8I