(Circular) (Six-base) MAPSORT of: pFN21AE1966.seq Check: 7295 from: 1 to: 6277 pFN21AE1966 6277 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3456 3456 Size: 6277 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3571 3620 1937 Size: 1634 49 4594 Fragments arranged by size: 4594 1634 49 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2811 3059 3198 3228 4340 5724 5992 1632 Size: 1179 248 139 30 1112 1384 268 1917 Fragments arranged by size: 1917 1384 1179 1112 268 248 139 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 3276 5176 6153 1203 Size: 2073 1900 977 1327 Fragments arranged by size: 2073 1900 1327 977 AgeI A'CCGG_T Cuts at: 5108 5108 Size: 6277 AhdI GACnn_n'nnGTC Cuts at: 5006 5006 Size: 6277 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6277 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6277 AlwNI CAG_nnn'CTG Cuts at: 3559 4020 5592 3559 Size: 461 1572 4244 Fragments arranged by size: 4244 1572 461 ApaLI G'TGCA_C Cuts at: 4334 5490 4334 Size: 1156 5121 ApoI r'AATT_y Cuts at: 1493 2312 2493 2569 3388 3586 3812 3964 6073 Size: 819 181 76 819 198 226 152 2109 Cuts at: 6073 1493 Size: 1697 Fragments arranged by size: 2109 1697 819 819 226 198 181 152 76 AseI AT'TA_AT Cuts at: 160 1032 4831 160 Size: 872 3799 1606 Fragments arranged by size: 3799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 3602 1870 Size: 73 1659 4545 Fragments arranged by size: 4545 1659 73 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3598 5053 618 Size: 325 217 627 1811 1455 1842 Fragments arranged by size: 1842 1811 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2414 2771 3596 1391 Size: 1023 357 825 4072 Fragments arranged by size: 4072 1023 825 357 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2461 2965 3065 928 Size: 971 562 504 100 4140 Fragments arranged by size: 4140 971 562 504 100 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4468 4502 1903 Size: 34 2531 34 3678 Fragments arranged by size: 3678 2531 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2849 3307 4169 5379 2849 Size: 458 862 1210 3747 Fragments arranged by size: 3747 1210 862 458 BclI T'GATC_A Cuts at: 1548 1548 Size: 6277 BfrBI ATG'CAT Cuts at: 3455 3455 Size: 6277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2921 4888 136 Size: 107 122 71 631 605 1249 1967 1525 Fragments arranged by size: 1967 1525 1249 631 605 122 107 71 BglII A'GATC_T Cuts at: 6272 6272 Size: 6277 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 3422 4338 5494 1790 Size: 1632 916 1156 2573 Fragments arranged by size: 2573 1632 1156 916 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4008 4966 442 Size: 416 53 83 399 24 2591 958 1753 Fragments arranged by size: 2591 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2539 2539 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4937 1239 Size: 3698 2579 Bpu10I CC'TnA_GC Cuts at: 3396 3396 Size: 6277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2057 2239 4402 5267 5565 2057 Size: 182 2163 865 298 2769 Fragments arranged by size: 2769 2163 865 298 182 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4940 882 Size: 617 3441 2219 Fragments arranged by size: 3441 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4068 493 Size: 711 2864 2702 Fragments arranged by size: 2864 2702 711 BsaBI GATnn'nnATC Cuts at: 6271 6271 Size: 6277 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4084 4466 5101 275 Size: 53 83 186 936 2551 382 635 1451 Fragments arranged by size: 2551 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2303 4710 5108 5382 5529 5940 2303 Size: 2407 398 274 147 411 2640 Fragments arranged by size: 2640 2407 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6052 Fragments arranged by size: 6052 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3375 1186 Size: 2189 4088 BseYI C'CCAG_C Cuts at: 3198 5480 5976 3198 Size: 2282 496 3499 Fragments arranged by size: 3499 2282 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5704 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3727 4488 5516 1540 Size: 275 186 1726 761 1028 2301 Fragments arranged by size: 2301 1726 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4338 4423 5494 4338 Size: 85 1071 5121 Fragments arranged by size: 5121 1071 85 BsmI GAATG_Cn' Cuts at: 3788 3881 3788 Size: 93 6184 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2414 2771 3422 3596 4338 4423 5494 Size: 399 624 357 651 174 742 85 1071 Cuts at: 5494 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 651 624 399 357 174 85 BspEI T'CCGG_A Cuts at: 2303 5940 2303 Size: 3637 2640 BspHI T'CATG_A Cuts at: 2137 4166 5896 2137 Size: 2029 1730 2518 Fragments arranged by size: 2518 2029 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3456 3632 844 Size: 2612 176 3489 Fragments arranged by size: 3489 2612 176 BsrBI CCG'CTC Cuts at: 1394 1823 2253 4164 1394 Size: 429 430 1911 3507 Fragments arranged by size: 3507 1911 430 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2037 2721 3409 3455 4772 4946 65 Size: 1183 789 684 688 46 1317 174 1396 Fragments arranged by size: 1396 1317 1183 789 688 684 174 46 BsrFI r'CCGG_y Cuts at: 1700 1810 4921 5108 1700 Size: 110 3111 187 2869 Fragments arranged by size: 3111 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 4337 5349 4337 Size: 1012 5265 BstAPI GCAn_nnn'nTGC Cuts at: 794 3092 3167 3440 794 Size: 2298 75 273 3631 Fragments arranged by size: 3631 2298 273 75 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6277 BstYI r'GATC_y Cuts at: 1972 2808 3607 3974 4359 4376 5828 5914 5926 Size: 836 799 367 385 17 1452 86 12 Cuts at: 5926 6272 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 836 799 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2445 2445 Size: 6277 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4786 Fragments arranged by size: 4786 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5538 Fragments arranged by size: 5538 683 56 BtsI GCAGTG_nn' Cuts at: 2080 3793 4587 4607 2080 Size: 1713 794 20 3750 Fragments arranged by size: 3750 1713 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6277 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6242 DraI TTT'AAA Cuts at: 3581 3581 Size: 6277 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5284 809 Size: 507 3968 1802 Fragments arranged by size: 3968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2083 2647 3724 Size: 54 1425 50 275 18 253 564 1077 Cuts at: 3724 4613 6242 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1077 889 564 275 253 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2508 3513 4207 1353 Size: 1155 1005 694 3423 Fragments arranged by size: 3423 1155 1005 694 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3108 4864 5238 5384 387 Size: 1064 138 1519 1756 374 146 1280 Fragments arranged by size: 1756 1519 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2811 3059 3198 3228 4340 4937 5724 Size: 393 1179 248 139 30 1112 597 787 Cuts at: 5724 5992 1239 Size: 268 1524 Fragments arranged by size: 1524 1179 1112 787 597 393 268 248 139 30 EcoRI G'AATT_C Cuts at: 2493 3586 3964 2493 Size: 1093 378 4806 Fragments arranged by size: 4806 1093 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2051 2816 2051 Size: 765 5512 FspI TGC'GCA Cuts at: 4783 4783 Size: 6277 HaeII r_GCGC'y Cuts at: 1678 5424 1678 Size: 3746 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2113 2145 2603 2635 4918 Size: 32 135 32 541 32 458 32 2283 Cuts at: 4918 4950 4992 5024 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2283 541 458 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3382 3621 3867 669 Size: 988 281 1444 239 246 3079 Fragments arranged by size: 3079 1444 988 281 246 239 HindIII A'AGCT_T Cuts at: 748 3210 748 Size: 2462 3815 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3426 3876 3426 Size: 450 5827 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3286 3289 3536 5391 5575 1305 Size: 1981 3 247 1855 184 2007 Fragments arranged by size: 2007 1981 1855 247 184 3 MscI TGG'CCA Cuts at: 10 64 1489 1832 2649 10 Size: 54 1425 343 817 3638 Fragments arranged by size: 3638 1425 817 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3375 4235 4594 4753 518 Size: 648 2209 860 359 159 2042 Fragments arranged by size: 2209 2042 860 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2482 2845 3230 3693 4370 5518 5763 1606 Size: 876 363 385 463 677 1148 245 2120 Fragments arranged by size: 2120 1148 876 677 463 385 363 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4786 Fragments arranged by size: 4786 939 552 NdeI CA'TA_TG Cuts at: 387 3499 387 Size: 3112 3165 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NsiI A_TGCA'T Cuts at: 3457 3457 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 3280 3635 5180 1132 Size: 97 2051 355 1545 2229 Fragments arranged by size: 2229 2051 1545 355 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6277 PciI A'CATG_T Cuts at: 3276 5176 3276 Size: 1900 4377 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6277 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2995 3027 4346 4378 5859 5891 2995 Size: 32 1319 32 1481 32 3381 Fragments arranged by size: 3381 1481 1319 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6277 PsiI TTA'TAA Cuts at: 3847 3847 Size: 6277 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2523 2555 2523 Size: 32 6245 PstI C_TGCA'G Cuts at: 830 2713 3229 3436 3629 830 Size: 1883 516 207 193 3478 Fragments arranged by size: 3478 1883 516 207 193 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 2482 2845 3230 2482 Size: 363 385 5529 Fragments arranged by size: 5529 385 363 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 4525 6237 4525 Size: 1712 4565 SfcI C'TryA_G Cuts at: 826 1045 2287 2526 2709 3140 3225 3432 3625 Size: 219 1242 239 183 431 85 207 193 Cuts at: 3625 4760 5441 5632 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1242 1135 681 431 239 219 207 193 191 183 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 3604 1872 Size: 1732 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2036 2254 4381 5282 5544 820 Size: 197 926 93 218 2127 901 262 1553 Fragments arranged by size: 2127 1553 926 901 262 218 197 93 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SspI AAT'ATT Cuts at: 5 52 4201 5 Size: 47 4149 2081 Fragments arranged by size: 4149 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6277 StyI C'CwwG_G Cuts at: 513 1065 2004 2501 2874 3716 513 Size: 552 939 497 373 842 3074 Fragments arranged by size: 3074 939 842 552 497 373 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4304 4321 4474 4659 5104 325 Size: 1255 2724 17 153 185 445 1498 Fragments arranged by size: 2724 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2677 4523 6235 Size: 274 80 33 51 1443 700 1846 1712 Cuts at: 6235 96 Size: 138 Fragments arranged by size: 1846 1712 1443 700 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3246 3278 6237 6269 3246 Size: 32 2959 32 3254 Fragments arranged by size: 3254 2959 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6277 XbaI T'CTAG_A Cuts at: 3613 3613 Size: 6277 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2923 2923 Size: 6277 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 3602 1870 Size: 1732 4545 XmnI GAAnn'nnTTC Cuts at: 4406 4406 Size: 6277 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI