################################## # NO CUT ENZYMES ( 188 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstYI BstZ17I Bsu36I Cac8I CelII ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI KspI MaeI MaeII MaeIII MfeI MflI MluI MnlI MroI MseI MspA1I MunI MvaI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp45I Tsp509I TspRI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AlwI 131 GGATC GGATC(4/5) AspI 49 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 16 GGTCC G^G[AT]CC BalI 212 TGGCCA TGG^CCA BbvI 41 GCTGC GCAGC(8/12) BcnI 98 CCGGG CC^[GC]GG BsaBI 222 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiYI 122 CCTGTCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 122 CCTGTCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 125 GTCTC GTCTC(1/5) BsmI 137 GAATGC GAATGC(1/-1) BsrFI 3 GCCGGT [GA]^CCGG[CT] BssKI 98 CCGGG ^CC[ACGT]GG BstAPI 227 GCATCCGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 215 CCATTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 3 GCCGGT [GA]^CCGG[CT] Cfr13I 16 GGTCC G^G[ACGT]CC DdeI 127 CTCAG C^T[ACGT]AG DpnII 132 GATC ^GATC DraII 15 GGGTCCC [GA]G^G[ACGT]CC[CT] EagI 95 CGGCCG C^GGCCG EarI 106 CTCTTC CTCTTC(1/4) EclXI 95 CGGCCG C^GGCCG Eco52I 95 CGGCCG C^GGCCG EcoNI 122 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 15 GGGTCCC [GA]G^G[ACGT]CC[CT] FauI 141 GCGGG CCCGC(4/6) HaeII 102 GGCGCT [GA]GCGC^[CT] Ksp632I 106 CTCTTC CTCTTC(1/4) MamI 222 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 132 GATC ^GATC MboII 107 TCTTC GAAGA(8/7) MluNI 212 TGGCCA TGG^CCA MscI 212 TGGCCA TGG^CCA NciI 98 CCGGG CC^[GC]GG NdeII 132 GATC ^GATC PflFI 49 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 15 GGGTCCC [GA]G^G[AT]CC[CT] SapI 105 GCTCTTC GCTCTTC(1/4) Sau3AI 132 GATC ^GATC Sau96I 16 GGTCC G^G[ACGT]CC ScrFI 98 CCGGG CC^[ACGT]GG TseI 41 GCTGC G^C[AT]GC Tth111I 49 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AluI 40 AGCT AG^CT 152 AGCT BanI 112 GGCACC G^G[CT][GA]CC 193 GGCACC BsiEI 48 CGACCG CG[GA][CT]^CG 95 CGGCCG BsmFI 17 GTCCC GGGAC(10/14) 30 GTCCC BstF5I 228 CATCC GGATG(2/0) 221 GGATG CfoI 80 GCGC GCG^C 103 GCGC DsaI 11 CCGTGG C^C[GA][CT]GG 83 CCGTGG FokI 228 CATCC GGATG(9/13) 221 GGATG HapII 4 CCGG C^CGG 98 CCGG HhaI 80 GCGC GCG^C 103 GCGC HinP1I 80 GCGC G^CGC 103 GCGC HpaII 4 CCGG C^CGG 98 CCGG MslI 204 CACATTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 216 CATTGGGATG MspI 4 CCGG C^CGG 98 CCGG TaqI 47 TCGA T^CGA 147 TCGA TthHB8I 47 TCGA T^CGA 147 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AciI BsaJI EaeI Fnu4HI HaeIII ItaI MwoI NlaIII NlaIV SfaNI