(Circular) (Six-base) MAPSORT of: pFN21AE5087.seq Check: 476 from: 1 to: 4942 pFN21AE5087 4942 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2752 278 Size: 53 83 186 936 1216 2468 Fragments arranged by size: 2468 1216 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2285 1937 Size: 348 4594 Acc65I G'GTAC_C Cuts at: 2263 2263 Size: 4942 AclI AA'CG_TT Cuts at: 1563 3069 3442 1563 Size: 1506 373 3063 Fragments arranged by size: 3063 1506 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3005 4389 4657 1632 Size: 1373 1384 268 1917 Fragments arranged by size: 1917 1384 1373 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4745 AflIII A'CryG_T Cuts at: 1203 3841 4818 1203 Size: 2638 977 1327 Fragments arranged by size: 2638 1327 977 AgeI A'CCGG_T Cuts at: 3773 3773 Size: 4942 AhdI GACnn_n'nnGTC Cuts at: 3671 3671 Size: 4942 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4942 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4942 AlwNI CAG_nnn'CTG Cuts at: 2685 4257 2685 Size: 1572 3370 ApaLI G'TGCA_C Cuts at: 2999 4155 2999 Size: 1156 3786 ApoI r'AATT_y Cuts at: 1493 2251 2477 2629 4738 1493 Size: 758 226 152 2109 1697 Fragments arranged by size: 2109 1697 758 226 152 AseI AT'TA_AT Cuts at: 160 1032 3496 160 Size: 872 2464 1606 Fragments arranged by size: 2464 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4942 AvaI C'yCGr_G Cuts at: 1870 1943 2267 1870 Size: 73 324 4545 Fragments arranged by size: 4545 324 73 BamHI G'GATC_C Cuts at: 2272 2639 2272 Size: 367 4575 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2117 2198 2263 3718 618 Size: 325 217 627 330 81 65 1455 1842 Fragments arranged by size: 1842 1455 627 330 325 217 81 65 BanII G_rGCy'C Cuts at: 1391 2261 1391 Size: 870 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3133 3167 1903 Size: 34 50 34 1112 34 3678 Fragments arranged by size: 3678 1112 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2834 4044 2834 Size: 1210 3732 BclI T'GATC_A Cuts at: 1548 1548 Size: 4942 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3553 136 Size: 107 122 71 631 605 1881 1525 Fragments arranged by size: 1881 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4937 4937 Size: 4942 BlpI GC'TnA_GC Cuts at: 2359 2359 Size: 4942 Bme1580I G_kGCm'C Cuts at: 1790 3003 4159 1790 Size: 1213 1156 2573 Fragments arranged by size: 2573 1213 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4942 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2673 3631 442 Size: 416 53 83 399 24 1256 958 1753 Fragments arranged by size: 1753 1256 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4942 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3602 1239 Size: 2363 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3067 3932 4230 3067 Size: 865 298 3779 Fragments arranged by size: 3779 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3605 882 Size: 617 2106 2219 Fragments arranged by size: 2219 2106 617 BsaAI yAC'GTr Cuts at: 493 1204 2733 493 Size: 711 1529 2702 Fragments arranged by size: 2702 1529 711 BsaBI GATnn'nnATC Cuts at: 2231 4936 2231 Size: 2705 2237 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2749 3131 3766 275 Size: 53 83 186 936 1216 382 635 1451 Fragments arranged by size: 1451 1216 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3375 3773 4047 4194 4605 3375 Size: 398 274 147 411 3712 Fragments arranged by size: 3712 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4717 Fragments arranged by size: 4717 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4942 BseYI C'CCAG_C Cuts at: 2037 4145 4641 2037 Size: 2108 496 2338 Fragments arranged by size: 2338 2108 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4369 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2056 2103 2392 3153 4181 1540 Size: 275 186 55 47 289 761 1028 2301 Fragments arranged by size: 2301 1028 761 289 275 186 55 47 BsiHKAI G_wGCw'C Cuts at: 3003 3088 4159 3003 Size: 85 1071 3786 Fragments arranged by size: 3786 1071 85 BsmI GAATG_Cn' Cuts at: 2148 2453 2546 2148 Size: 305 93 4544 Fragments arranged by size: 4544 305 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2261 3003 3088 4159 1391 Size: 399 471 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 471 399 85 BspEI T'CCGG_A Cuts at: 4605 4605 Size: 4942 BspHI T'CATG_A Cuts at: 2831 4561 2831 Size: 1730 3212 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2297 844 Size: 1453 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2829 1394 Size: 429 1006 3507 Fragments arranged by size: 3507 1006 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3437 3611 65 Size: 1183 2189 174 1396 Fragments arranged by size: 2189 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2008 3586 3773 1700 Size: 110 198 1578 187 2869 Fragments arranged by size: 2869 1578 198 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4942 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4942 BssSI C'ACGA_G Cuts at: 3002 4014 3002 Size: 1012 3930 BstAPI GCAn_nnn'nTGC Cuts at: 794 2238 794 Size: 1444 3498 BstBI TT'CG_AA Cuts at: 3746 3746 Size: 4942 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4942 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2227 2011 Size: 216 4726 BstYI r'GATC_y Cuts at: 1972 2272 2639 3024 3041 4493 4579 4591 4937 Size: 300 367 385 17 1452 86 12 346 Cuts at: 4937 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 300 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2016 2088 513 Size: 552 951 72 3367 Fragments arranged by size: 3367 951 552 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4203 Fragments arranged by size: 4203 683 56 BtsI GCAGTG_nn' Cuts at: 2458 3252 3272 2458 Size: 794 20 4128 Fragments arranged by size: 4128 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4942 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4907 DraI TTT'AAA Cuts at: 2246 2246 Size: 4942 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3949 809 Size: 507 2633 1802 Fragments arranged by size: 2633 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2100 2217 Size: 54 1425 50 275 18 183 87 117 Cuts at: 2217 2389 3278 4907 8 Size: 172 889 1629 43 Fragments arranged by size: 1629 1425 889 275 183 172 117 87 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2100 2389 1537 Size: 275 288 289 4090 Fragments arranged by size: 4090 289 288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2117 2872 1353 Size: 764 755 3423 Fragments arranged by size: 3423 764 755 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3529 3903 4049 387 Size: 1064 138 1940 374 146 1280 Fragments arranged by size: 1940 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3005 3602 4389 4657 1239 Size: 393 1373 597 787 268 1524 Fragments arranged by size: 1524 1373 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2131 2131 Size: 4942 EcoO109I rG'GnC_Cy Cuts at: 2021 2021 Size: 4942 EcoRI G'AATT_C Cuts at: 2251 2629 2251 Size: 378 4564 FspI TGC'GCA Cuts at: 3448 3448 Size: 4942 HaeII r_GCGC'y Cuts at: 1678 2111 4089 1678 Size: 433 1978 2531 Fragments arranged by size: 2531 1978 433 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3583 3615 3657 3689 1373 Size: 32 135 32 2011 32 42 32 2626 Fragments arranged by size: 2626 2011 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2286 2532 669 Size: 988 281 348 246 3079 Fragments arranged by size: 3079 988 348 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4942 HpaI GTT'AAC Cuts at: 2532 2532 Size: 4942 KpnI G_GTAC'C Cuts at: 2267 2267 Size: 4942 MfeI C'AATT_G Cuts at: 2541 2541 Size: 4942 MluI A'CGCG_T Cuts at: 4818 4818 Size: 4942 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4056 4240 1305 Size: 2751 184 2007 Fragments arranged by size: 2751 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2219 10 Size: 54 1425 343 387 2733 Fragments arranged by size: 2733 1425 387 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2213 2225 2900 3259 3418 518 Size: 648 843 204 12 675 359 159 2042 Fragments arranged by size: 2042 843 675 648 359 204 159 12 MspA1I CmG'CkG Cuts at: 1606 2358 3035 4183 4428 1606 Size: 752 677 1148 245 2120 Fragments arranged by size: 2120 1148 752 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4390 NdeI CA'TA_TG Cuts at: 387 387 Size: 4942 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4942 NotI GC'GGCC_GC Cuts at: 2389 2389 Size: 4942 NspI r_CATG'y Cuts at: 1132 1229 2300 3845 1132 Size: 97 1071 1545 2229 Fragments arranged by size: 2229 1545 1071 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4942 PciI A'CATG_T Cuts at: 3841 3841 Size: 4942 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4942 PmeI GTTT'AAAC Cuts at: 2246 2246 Size: 4942 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3011 3043 4524 4556 3011 Size: 32 1481 32 3397 Fragments arranged by size: 3397 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2021 2021 Size: 4942 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4942 PsiI TTA'TAA Cuts at: 2512 2512 Size: 4942 PstI C_TGCA'G Cuts at: 830 2294 830 Size: 1464 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4942 SalI G'TCGA_C Cuts at: 1936 2284 1936 Size: 348 4594 SanDI GG'GwC_CC Cuts at: 2021 2021 Size: 4942 SapI GCTCTTCn'nnn_ Cuts at: 2117 2117 Size: 4942 SbfI CC_TGCA'GG Cuts at: 2294 2294 Size: 4942 ScaI AGT'ACT Cuts at: 3190 4902 3190 Size: 1712 3230 SfcI C'TryA_G Cuts at: 826 1045 2290 3425 4106 4297 826 Size: 219 1245 1135 681 191 1471 Fragments arranged by size: 1471 1245 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4942 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4942 SmaI CCC'GGG Cuts at: 1872 2269 1872 Size: 397 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3046 3947 4209 820 Size: 197 926 1103 901 262 1553 Fragments arranged by size: 1553 1103 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2733 493 Size: 2240 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4942 SphI G_CATG'C Cuts at: 1132 2300 1132 Size: 1168 3774 SspI AAT'ATT Cuts at: 5 52 2866 5 Size: 47 2814 2081 Fragments arranged by size: 2814 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4942 StyI C'CwwG_G Cuts at: 513 1065 2381 513 Size: 552 1316 3074 Fragments arranged by size: 3074 1316 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2969 2986 3139 3324 3769 325 Size: 1255 1389 17 153 185 445 1498 Fragments arranged by size: 1498 1389 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3188 4900 96 Size: 274 80 33 51 1443 1211 1712 138 Fragments arranged by size: 1712 1443 1211 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4902 4934 4902 Size: 32 4910 Tth111I GACn'n_nGTC Cuts at: 1316 2057 1316 Size: 741 4201 XbaI T'CTAG_A Cuts at: 2278 2278 Size: 4942 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4942 XmaI C'CCGG_G Cuts at: 1870 2267 1870 Size: 397 4545 XmnI GAAnn'nnTTC Cuts at: 3071 3071 Size: 4942 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2750 276 Size: 53 83 186 936 1216 2468 Fragments arranged by size: 2468 1216 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI