################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MluI MroI MseI MslI MunI NarI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccII 376 CGCG CG^CG AlwNI 360 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 76 CCTGAGG CC^T[ACGT]AGG AspHI 379 GTGCTC G[AT]GC[AT]^C AvaII 257 GGACC G^G[AT]CC BanI 309 GGCACC G^G[CT][GA]CC BbsI 37 GTCTTC GAAGAC(2/6) BcnI 254 CCCGG CC^[GC]GG BglII 211 AGATCT A^GATCT BlpI 381 GCTCAGC GC^T[ACGT]AGC BmyI 379 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 381 GCTCAGC GC^T[ACGT]AGC BpuAI 37 GTCTTC GAAGAC(2/6) BsaJI 253 CCCCGG C^C[ACGT][ACGT]GG BsiHKAI 379 GTGCTC G[AT]GC[AT]^C BsmAI 148 GTCTC GTCTC(1/5) BsmFI 139 GTCCC GGGAC(10/14) Bsp1286I 379 GTGCTC G[AGT]GC[ACT]^C BspMI 68 ACCTGC ACCTGC(4/8) BsrFI 306 GCCGGC [GA]^CCGG[CT] BstNI 21 CCTGG CC^[AT]GG BstUI 376 CGCG CG^CG BstXI 26 CCACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 211 AGATCT [GA]^GATC[CT] Bsu36I 76 CCTGAGG CC^T[ACGT]AGG CelII 381 GCTCAGC GC^T[ACGT]AGC Cfr10I 306 GCCGGC [GA]^CCGG[CT] Eco81I 76 CCTGAGG CC^T[ACGT]AGG EcoRII 21 CCTGG ^CC[AT]GG HphI 8 TCACC GGTGA(8/7) MaeIII 7 GTCAC ^GT[ACGT]AC MflI 211 AGATCT [GA]^GATC[CT] MspA1I 294 CAGCTG C[AC]G^C[GT]G MvaI 21 CCTGG CC^[AT]GG MvnI 376 CGCG CG^CG NaeI 306 GCCGGC GCC^GGC NciI 254 CCCGG CC^[GC]GG NgoMIV 306 GCCGGC G^CCGGC PvuII 294 CAGCTG CAG^CTG TaqI 232 TCGA T^CGA Tsp45I 7 GTCAC ^GT[GC]AC TthHB8I 232 TCGA T^CGA XhoII 211 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AluI 86 AGCT AG^CT 295 AGCT BalI 23 TGGCCA TGG^CCA 281 TGGCCA BseRI 117 CTCCTC GAGGAG(10/8) 79 GAGGAG BsrI 132 ACTGG ACTGG(1/-1) 342 CCAGT BssKI 21 CCTGG ^CC[ACGT]GG 254 CCCGG BstAPI 290 GCAACAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 359 GCAGCGCCTGC BstF5I 3 GGATG GGATG(2/0) 135 GGATG Cfr13I 251 GGCCC G^G[ACGT]CC 257 GGACC DpnII 183 GATC ^GATC 212 GATC EaeI 23 TGGCCA [CT]^GGCC[GA] 281 TGGCCA FokI 3 GGATG GGATG(9/13) 135 GGATG HaeII 159 GGCGCT [GA]GCGC^[CT] 361 AGCGCC HapII 255 CCGG C^CGG 307 CCGG HpaII 255 CCGG C^CGG 307 CCGG MboI 183 GATC ^GATC 212 GATC MluNI 23 TGGCCA TGG^CCA 281 TGGCCA MscI 23 TGGCCA TGG^CCA 281 TGGCCA MspI 255 CCGG C^CGG 307 CCGG NdeII 183 GATC ^GATC 212 GATC Sau3AI 183 GATC ^GATC 212 GATC Sau96I 251 GGCCC G^G[ACGT]CC 257 GGACC ScrFI 21 CCTGG CC^[ACGT]GG 254 CCCGG TspRI 54 CACTG CA[GC]TG[ACGT][ACGT]^ 343 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AciI BbvI Cac8I CfoI DdeI FauI Fnu4HI HaeIII HhaI HinP1I ItaI MboII MnlI MwoI NlaIV TseI