################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfaI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI Hin1I HindIII HinfI HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MflI MluNI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AfaI 384 GTAC GT^AC AflII 88 CTTAAG C^TTAAG AlwI 524 GATCC GGATC(4/5) ApaI 131 GGGCCC GGGCC^C AvaI 328 CTCGGG C^[CT]CG[GA]G AvaII 459 GGACC G^G[AT]CC AviII 53 TGCGCA TGC^GCA BciVI 335 GTATCC GTATCC(6/5) BcnI 129 CCGGG CC^[GC]GG BfrI 88 CTTAAG C^TTAAG BpmI 178 CTCCAG CTGGAG(16/14) BsaI 259 GGTCTC GGTCTC(1/5) BsmAI 260 GTCTC GTCTC(1/5) BsmI 310 GCATTC GAATGC(1/-1) BsoBI 328 CTCGGG C^[CT]CG[GA]G BspHI 149 TCATGA T^CATGA BspLU11I 380 ACATGT A^CATGT BsrDI 578 GCAATG GCAATG(2/0) BstF5I 441 CATCC GGATG(2/0) EaeI 80 TGGCCG [CT]^GGCC[GA] EarI 160 CTCTTC CTCTTC(1/4) EcoNI 564 CCTCTATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FokI 441 CATCC GGATG(9/13) FspI 53 TGCGCA TGC^GCA HaeII 59 AGCGCC [GA]GCGC^[CT] HgaI 398 GCGTC GACGC(5/10) HincII 40 GTCAAC GT[CT]^[GA]AC HindII 40 GTCAAC GT[CT]^[GA]AC Ksp632I 160 CTCTTC CTCTTC(1/4) MaeIII 393 GTCAC ^GT[ACGT]AC MboII 161 TCTTC GAAGA(8/7) MluI 396 ACGCGT A^CGCGT MseI 89 TTAA T^TAA MslI 477 CACTGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 469 CCGCTG C[AC]G^C[GT]G NciI 129 CCGGG CC^[GC]GG NspI 380 ACATGT [GA]CATG^[CT] PpuMI 458 GGGACCC [GA]G^G[AT]CC[CT] RcaI 149 TCATGA T^CATGA RsaI 384 GTAC GT^AC SmlI 88 CTTAAG C^T[CT][GA]AG Tru9I 89 TTAA T^TAA Tsp45I 393 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 141 CGCG CG^CG 397 CGCG AccIII 98 TCCGGA T^CCGGA 164 TCCGGA AflIII 380 ACATGT A^C[GA][CT]GT 396 ACGCGT AluI 17 AGCT AG^CT 188 AGCT BanII 23 GGGCTC G[GA]GC[CT]^C 131 GGGCCC BsaJI 327 CCTCGG C^C[ACGT][ACGT]GG 451 CCCAGG BsaWI 98 TCCGGA [AT]^CCGG[AT] 164 TCCGGA BseAI 98 TCCGGA T^CCGGA 164 TCCGGA BsmFI 458 GGGAC GGGAC(10/14) 626 GTCCC BspEI 98 TCCGGA T^CCGGA 164 TCCGGA BstUI 141 CGCG CG^CG 397 CGCG Cac8I 56 GCAAGC GC[ACGT]^[ACGT]GC 204 GCAGGC DpnII 375 GATC ^GATC 524 GATC DraII 131 GGGCCCC [GA]G^G[ACGT]CC[CT] 458 GGGACCC EcoO109I 131 GGGCCCC [GA]G^G[ACGT]CC[CT] 458 GGGACCC FauI 9 CCCGC CCCGC(4/6) 468 CCCGC HphI 248 TCACC GGTGA(8/7) 519 TCACC MboI 375 GATC ^GATC 524 GATC MroI 98 TCCGGA T^CCGGA 164 TCCGGA MvnI 141 CGCG CG^CG 397 CGCG NdeII 375 GATC ^GATC 524 GATC Sau3AI 375 GATC ^GATC 524 GATC TspRI 47 CACTG CA[GC]TG[ACGT][ACGT]^ 477 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI BbvI BmyI BsiYI BslI Bsp1286I BssKI BstNI CfoI Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspI MvaI MwoI NlaIII NlaIV PstI Sau96I ScrFI SfcI TseI